Abstract：
:Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for industrial-scale protein production exposes bacteria (harboring genes of interest) to agroinfiltration medium that is devoid of nutrients and carbon sources for prolonged periods of time (possibly upwards of 24 h). Such conditions may negatively influence bacterial viability, infectivity of plant cells, and target protein production. Here, we explored the role of timing in bacterial culture preparation for agroinfiltration using mass spectrometry-based proteomics to define changes in cellular processes. We observed distinct profiles associated with bacterial treatment conditions and exposure timing, including significant changes in proteins involved in pathogenesis, motility, and nutrient acquisition systems as the bacteria adapt to the new environment. These data suggest a progression towards increased cellular remodelling over time. In addition, we described changes in growth- and environment-specific processes over time, underscoring the interconnectivity of pathogenesis and chemotaxis-associated proteins with transport and metabolism. Overall, our results have important implications for the production of transiently expressed target protein products, as prolonged exposure to agroinfiltration medium suggests remodelling of the bacterial proteins towards enhanced infection of plant cells.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Prudhomme N,Pastora R,Muselius B,McLean MD,Cossar D,Geddes-McAlister Jdoi
10.1139/cjm-2020-0239subject
Has Abstractpub_date
2021-01-01 00:00:00pages
85-97issue
1eissn
0008-4166issn
1480-3275journal_volume
67pub_type
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journal_title：Canadian journal of microbiology
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更新日期：1980-10-01 00:00:00
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更新日期：1979-11-01 00:00:00
