Abstract：
:Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipid, supplied by the alpha globulin, were required for maximal growth of these two oral treponemes. The lipid component was investigated by adding sodium salts of long-chain fatty acids to the basal medium supplemented with 0.4% delipified alpha globulin. The lipid component of alpha globulin was replaced by either oleic acid (cis-18:1(9)) or by elaidic acid (trans- 18:1 (9)0. No other saturated or unsaturated fatty acid tested could support good growth. Tween 80 (polysorbitan monooleate) was the only Tween compound able to support maximal growth of T. denticola. The cellular lipids of T. denticola, grown with oleate in broth supplemented with 0.4% delipified alpha globulin, were extracted and analyzed by gas chromatography. The principle fatty acids were myristic, pentadecanoic, and palmitic acids. Lesser amounts of oleic acid, eicosadienoic acid, and an unidentified fatty acid (retention time, 88 min) were also detected. Treponema denticola appears to be capable of limited synthesis of cellular fatty acids such as myristic, pentadecanoic, and palmitic acids from oleic acid.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Van Horn KG,Smibert RMdoi
10.1139/m82-051subject
Has Abstractpub_date
1982-03-01 00:00:00pages
344-50issue
3eissn
0008-4166issn
1480-3275journal_volume
28pub_type
杂志文章abstract：:The origin of the eukaryotic nucleus is difficult to reconstruct. While eukaryotic organelles (chloroplast, mitochondrion) are eubacterial endosymbionts, the source of nuclear genes has been obscured by multiple nucleotide substitutions. Using evolutionary parsimony, a newly developed rate-invariant treeing algorithm,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-017
更新日期：1989-01-01 00:00:00
abstract：:An active in vitro protein-synthesizing system has been developed from Rhodospirillum rubrum grown under either photosynthetic or heterotrophic conditions. A protease activity has been found in both of these systems, and this activity can be readily inactivated by treating the cells with KCl and phenylmethyl sulfonylf...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-042
更新日期：1976-02-01 00:00:00
abstract：:Pathogenic and nonpathogenic isolates of Venturia inaequalis were grown in liquid culture. Hyphae were treated with two types of fimbrial antiserum (AU- and AV-1) and examined by immunofluorescent microscopy, in order to establish the distribution of fimbrial epitopes in whole cell mounts. The AV-1 antiserum was speci...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-061
更新日期：2000-09-01 00:00:00
abstract：:A high molecular component of a saline extract derived from Listeria monocytogenes contained amino acids, carbohydrates, and phosphorus. The same fraction was capable of promoting both the in vitro mitogenic and adjuvant activities and the in vivo immunosuppressive activity displayed by the crude extract. The material...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-204
更新日期：1982-12-01 00:00:00
abstract：:Sera from mice which have been vaccinated with BCG and challenged with old tuberculin contain gamma interferon. These same sera also express antibacterial activity. Using Staphylococcus aureus we demonstrated that its growth was inhibited at dilutions of sera as high as 1:320. A 4% concentration of sera reduced the gr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-084
更新日期：1986-05-01 00:00:00
abstract：:Evidence has shown that individuals exposed to indoor toxic molds for extended periods of time have elevated risk of developing numerous respiratory illnesses. It is not clear at the cellular level what impact mold exposure has on the immune system. Herein, we show that 2 fungal volatiles (E)-2-octenal and oct-1-en-3-...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0708
更新日期：2014-01-01 00:00:00
abstract：:Species of Enterobacter and Serratia were examined for deoxyribonucleic acid relatedness to Klebsielleae, to atypical erwiniae, and to other members of Enterobacteriaceae. Deoxyribonucleic acid hybridization and then hydroxyapatite chromatography was the technique used to assess relatedness. Strains of Enterobacter cl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-018
更新日期：1976-02-01 00:00:00
abstract：:The addition of various concentrations of monensin (1,5, and 10 micrograms/mL) to the culture medium inhibits the fungal growth and perturbs exopolysaccharides secretion, provoking a decrease of production in Botrytis cinerea and an increase in Sclerotium rolfsii. The ionophore induces also modifications in both polym...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-124
更新日期：1996-09-01 00:00:00
abstract：:Despite the interactions known to occur between various lower respiratory tract pathogens and alveolar epithelial cells (AECs), few reports examine factors influencing the interplay between Mycobacterium tuberculosis bacilli and AECs during infection. Importantly, in vitro studies have demonstrated that the M. tubercu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0528
更新日期：2015-12-01 00:00:00
abstract：:The role the environment has on populations of Pythium and Mesocriconema spp. was investigated at 5 golf course locations in east central Alabama. Every 4 to 5 weeks soil samples were collected from 3 golf greens on each of the 5 golf courses. Environmental data, including air and soil temperature, pH and relative hum...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-005
更新日期：2005-04-01 00:00:00
abstract：:Several restriction systems were detected in different strains of Streptomyces antibioticus by using actinophages as biological indicators. Adsorption of phages to the bacteria, together with the study of the efficiency of plating gave an initial indication of restriction in three strains. The alternation of efficienc...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-177
更新日期：1985-10-01 00:00:00
abstract：:Separation of Vero cell membrane in a discontinuous sucrose gradient reveals five fractions. After infection a sixth fraction appears. It contains virioins but mostly modified membranes with subunits 5-6 nm in diameter, probably the hemagglutinin. None of the enzymes used was associated with this fraction. No modifica...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-103
更新日期：1975-05-01 00:00:00
abstract：:Fermentative hydrogen production was carried out by Enterobacter cloacae DM11, using glucose as the substrate. The effects of initial substrate concentration, initial medium pH, and temperature were investigated. Results showed that at an initial glucose concentration of 1.0% (m/v), the molar yield of hydrogen was 3.3...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-005
更新日期：2006-06-01 00:00:00
abstract：:The environmental distribution of Giardia spp. and Cryptosporidium spp. is dependent upon human, agricultural, and wildlife sources. The significance of each source with regard to the presence of parasites in the environment is unknown. This 2-year study examined parasite prevalence in human sewage influent, wildlife,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-047
更新日期：2002-06-01 00:00:00
abstract：:Incubation of 4'-chloro-4-biphenylol with a mushroom tyrosinase preparation gave the catechol, 4'-chloro-3,4-biphenyldiol as the sole in vitro metabolite. This compound was identical with the major rat urinary metabolite of 4'-chloro-4-biphenylol and thus confirms the structure assigned to the metabolite. This result ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-014
更新日期：1976-01-01 00:00:00
abstract：:In this work, we disrupted one of three putative phosphatidylinositol phospholipase C genes of Aspergillus nidulans and studied its effect on carbon source sensing linked to vegetative mitotic nuclear division. We showed that glucose does not affect nuclear division rates during early vegetative conidial germination (...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-034
更新日期：2011-07-01 00:00:00
abstract：:Fourteen Lactococcus lactis strains showing inhibitory activity against Listeria innocua SICC 4202 were isolated from different French raw milks and raw milk cheeses and screened for bacteriocin production by the triple layer method under conditions that eliminate the effects of lactic acid and hydrogen peroxide. Thre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-114
更新日期：1995-09-01 00:00:00
abstract：:The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC signific...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-018
更新日期：2009-06-01 00:00:00
abstract：:The cellular functions are strongly influenced by the composition of the environment. In particular, phenotypes of microbial strains are modulated by concentrations of ions in the culture medium, and differences in element levels may be responsible for a phenotypic variability observed when microbial strains are grown...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-012
更新日期：2007-04-01 00:00:00
abstract：:A baseline serotype distribution was established by age and region for 2058 invasive Streptococcus pneumoniae isolates collected during the implementation period of the 13-valent pneumococcal conjugate vaccine (PCV13) program in many parts of Canada in 2010. Serotypes 19A, 7F, and 3 were the most prevalent in all ag...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-073
更新日期：2012-08-01 00:00:00
abstract：:Caldibacillus debilis strains GB1 and Tf display distinct phenotypes. Caldibacillus debilis GB1 is capable of anaerobic growth and can synthesize ethanol while C. debilis Tf cannot. Comparison of the GB1 and Tf genome sequences revealed that the genomes were highly similar in gene content and showed a high level of sy...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0464
更新日期：2019-06-01 00:00:00
abstract：:The natural reservoirs and biological characteristics of pathogenic populations of both subspecies of Photobacterium damselae in aquatic habitants remain unclear because of difficulties in obtaining pathogenic strains from the environment. In the present study, we assessed the occurrence of Photobacterium damselae sub...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-021
更新日期：2011-05-01 00:00:00
abstract：:The physiological characteristics and fatty acid composition of 40 strains of Yarrowia lipolytica isolated from irradiated poultry meat, commercial chilled foods, the superficial water of lagoons of an Italian river delta, and commercial light butter were compared. Discriminant analysis of the results permitted the 40...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-008
更新日期：1994-01-01 00:00:00
abstract：:Strain-specific fluorescent antibodies were prepared for three isolates of Aereomonas hydrophila. These antisera were reacted with 255 other A. hydrophila isolates, and their immunofluorescence was measured quantitatively by photometric fluorescence microscopy. Only 27.5% of the isolates reacted with the prepared anti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-024
更新日期：1980-02-01 00:00:00
abstract：:3-O-Methyl-D-glucose (3-O-MeGlc) or a mixture of 3-O-MeGlc and glucose stimulate invertase, beta-glucosidase, alpha-glucosidase, and alpha-galactosidase production by Cryptococcus laurentii. They also increase invertase and alpha-glucosidase production by Saccharomyces cerevisiae. The stimulatory effect of 3-O-MeGlc i...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-215
更新日期：1980-11-01 00:00:00
abstract：:Heat activation (70 degrees C for 20 min) resulted in alteration in structural proteins and enzymes found in Bacillus cereus spore coats. The three notable changes were increased glycosylation of coat proteins, alteration in polypeptide pattern on sodium dodecyl sulfate - polyacrylamide gels, and an increase in free S...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-062
更新日期：1981-04-01 00:00:00
abstract：:Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0239
更新日期：2021-01-01 00:00:00
abstract：:The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-239
更新日期：1976-11-01 00:00:00
abstract：:A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-162
更新日期：1997-12-01 00:00:00
abstract：:Rabbit antisera prepared to meningococcal serogroups Y and W135 strains were compared with horse antisera using the antiserum agar method (ASA) for the serogroup identification of Neisseria meningitidis. Thirty-seven group Y strains formed immunoprecipitates with the Y rabbit serum only, whereas the same Y strains for...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-109
更新日期：1980-05-01 00:00:00
