Abstract：
:Sera from mice which have been vaccinated with BCG and challenged with old tuberculin contain gamma interferon. These same sera also express antibacterial activity. Using Staphylococcus aureus we demonstrated that its growth was inhibited at dilutions of sera as high as 1:320. A 4% concentration of sera reduced the growth rate of the S. aureus from 1.6 to 0.6 doubling times per hour. The activity was stable at 56 degrees C but destroyed by 80 degrees C. It was nondialysable and destroyed by acid conditions (pH 2.0) and by the proteolytic enzymes trypsin and chymotrypsin. Antibodies to gamma interferon neutralized the antiviral activity but not the antibacterial activity. Mitogen-induced and virus-induced interferons did not have activity. We subsequently demonstrated that the factor could be induced in mice using BCG without the secondary old tuberculin challenge. No gamma interferon was found in the sera of mice given BCG without old tuberculin. These findings indicate that the antibacterial activity of these sera is not dependent on the presence of gamma interferon. We will continue to work to characterize and identify the antibacterial component in these sera.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Moulton RG,Gerner GDdoi
10.1139/m86-084subject
Has Abstractpub_date
1986-05-01 00:00:00pages
442-5issue
5eissn
0008-4166issn
1480-3275journal_volume
32pub_type
杂志文章abstract：:Five strains of Haemophilus pleuropneumoniae, out of eight strains tested, produced extracellular haemolysin(s) when grown in liquid culture in the presence, but not in the absence, of RNA. The haemolysin produced by the neotype strain was unstable, heat labile, and sensitive to degradation by pronase, trypsin, and ch...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-085
更新日期：1985-05-01 00:00:00
abstract：:Epiphytic tracer bacteris (a Pseudomonas sp. and an Arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. Of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. Seed from two of these pods also carried tracers. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-064
更新日期：1976-03-01 00:00:00
abstract：:Five media and procedures were compared for their recovery of fecal streptococci on membrane filters. Growth and numbers of a variety of pure cultures were compared, and recoveries from raw sewage and sewage effluent were determined on the five media. A total of 512 isolates were identified and the specificity of each...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-219
更新日期：1980-11-01 00:00:00
abstract：:Fine hair-like appendages on the cell walls of the another smut Ustilago violacea are described. These hairs are termed fimbriae because of their close similarity to the fimbriae (pili) found on certain Gram-negative bacteria. Cells of U. violacea may carry more than 200 fimbriae varying in length from about 0.5 mum t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-076
更新日期：1975-04-01 00:00:00
abstract：:Members of the genus Bacillus and related spore-forming genera are ubiquitous. However, Bacillus-like species isolated from marine sediments have attracted less interest than their terrestrial relatives. Here, we investigated the diversity of Bacillus-like bacterial communities in the sediments of the Bamenwan mangrov...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0449
更新日期：2017-03-01 00:00:00
abstract：:Enzymes induced by two Rhizobium trifolii bacteriophages caused depolymerization of exopolysaccharides from most R. trifolii and R. leguminosarum strains tested, but did not, in general, attack the exopolysaccharides of R. meliloti, the slow-growing rhizobia, or Agrobacterium. Ca2+ and (or) Mg2+ were required for enzy...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-239
更新日期：1975-10-01 00:00:00
abstract：:Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Auxotrophs in three different races of Pyricularia oryzae were obtained following ultraviolet mutagenesis, and tested for complementarity on minimal medium. Prototrophic growth resulted between combinations of different auxotrophs of single strains, but not between strains. The growth did not result form crossfeeding,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-079
更新日期：1976-04-01 00:00:00
abstract：:This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjec...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-083
更新日期：2007-10-01 00:00:00
abstract：:The presence of other soil microorganisms might influence the ability of rhizobacterial inoculants to promote plant growth either by reducing contact between the inoculant and the plant root or by interfering with the mechanism(s) involved in rhizobacterially mediated growth promotion. We conducted the following exper...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-080
更新日期：2001-09-01 00:00:00
abstract：:The poly(3-hydroxybutyrate) (PHB) depolymerase structural gene of Comamonas sp. (phaZCsp) was cloned in Escherichia coli and identified by halo formation on PHB-containing solid medium. The nucleotide sequence of a 1719 base pair MboI fragment was determined and contained one large open reading frame (ORF1, 1542 base ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-183
更新日期：1995-01-01 00:00:00
abstract：:The search for new antimicrobial compounds involves finding novel sources of chemotherapeutic compounds or manipulating and combining structures from existing molecules. Small antimicrobial peptides (AMPs) are components of innate immune defenses characterized in greatest detail in insect-derived AMPs. We have generat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-001
更新日期：2009-05-01 00:00:00
abstract：:Rhizobia are used exclusively in agricultural systems for enhancing the ability of legumes to fix atmospheric nitrogen. Knowledge about the indigenous population is necessary for the selection and application of inoculant strains. In this study, we have assessed the genetic diversity of Bradyrhizobium strains isolated...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-139
更新日期：2001-02-01 00:00:00
abstract：:The aim of this study was to determine the effect of the rhizosphere of maize on the diversity of denitrifying bacteria. Community structure comparison was performed by constructing a collection of isolates recovered from bulk and maize planted soil. A total of 3240 nitrate-reducing isolates were obtained and 188 of t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-037
更新日期：2004-07-01 00:00:00
abstract：:Attempts at improving chemical fixation for electron-microscopic observation of the capsule of Klebsiella pneumoniae were made. The capsule was preserved by using alcian blue - lanthanum and tris-(1-aziridinyl) phosphine oxide (TAPO) - aldehyde - osmium procedures. Despite the different retention of the overall capsul...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-102
更新日期：1977-06-01 00:00:00
abstract：:The morphology of Clostridium pasteurianum cells grown on 10(-2) M SO32- showed significant alteration in cell shape and the absence of the electron translucent reserve polysaccharide (amylopectin) when compared to sulfate-grown cells. At the lower sulfite concentrations (10(-3) and 10(-4)M SO32-) the cells showed the...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-036
更新日期：1976-02-01 00:00:00
abstract：:Shiga-toxin-producing Escherichia coli (STEC) represent a major concern for waterborne disease outbreaks associated with consumption of contaminated groundwater. Over 4 million people rely on private groundwater systems as their primary drinking water source in Canada; many of these systems do not meet current standar...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/cjm-2019-0329
更新日期：2020-05-01 00:00:00
abstract：:The acetylene-reduction assay was used for in situ and laboratory assessment of biological nitrogen fixation in the acidic, waterlogged, muskeg ecosystem of the southern James Bay area, in the region of Moosonee, Ontario. In situ assays and subsequent laboratory experimentation revealed that nitrogenase activity was p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-130
更新日期：1976-07-01 00:00:00
abstract：:Biological nitrogen fixation (BNF) can be improved by optimizing the interaction between the rhizobial inoculant and pea (Pisum sativum L.), leading to increased productivity and reduced nitrogen (N) fertilizer use. Eight Rhizobium leguminosarum bv. viciae strains were used to inoculate the super-nodulating pea mutant...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2017-0281
更新日期：2017-11-01 00:00:00
abstract：:Unicellular green alga represents not only a convenient model for its biochemical and physiological studies but also a sensitive system to test the effects of various environmental factors. Algae cells of two strains, SA-3 strain (exsymbiotic from Paramecium bursaria) and Chlorella vulgaris c-27, were asynchronously c...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-143
更新日期：2002-02-01 00:00:00
abstract：:Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-051
更新日期：1982-03-01 00:00:00
abstract：:Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-148
更新日期：1976-07-01 00:00:00
abstract：:A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-027
更新日期：1993-02-01 00:00:00
abstract：:Nodulation of soybeans by indigenous and inoculum strains of Bradyrhizobium japonicum was studied in field experiments in Wisconsin from 1983 to 86. Aqueous suspensions of bacteria were applied to seeds at the time of planting at levels of 7 × 10(7)-10(10) bacteria per 2.5-cm row. The predominant indigenous serogroup ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-136
更新日期：1990-11-01 00:00:00
abstract：:Listeria grayi is a nonpathogenic Gram-positive bacterium that demonstrates considerable similarities to other members in the genus Listeria, including the foodborne human pathogen Listeria monocytogenes and the animal pathogen Listeria ivanovii. A rapid diagnostic test to identify and diagnose listeriosis would be va...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-108
更新日期：2005-01-01 00:00:00
abstract：:Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-021
更新日期：1989-01-01 00:00:00
abstract：:A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w99-055
更新日期：1999-08-01 00:00:00
abstract：:The adherence of Streptococcus pneumoniae to epithelial (A549) lung cells was studied and the bacterial binding distribution was found to be nonrandom (non-Gaussian). Analysis of the dependency of bacterial binding on the cell cycle of A549 cells revealed that approximately 1.8 times more bacteria bind to G2 cells tha...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:Uptake of amino acids by Bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. A high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. All amino acids except proline we...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-180
更新日期：1979-10-01 00:00:00
abstract：:Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal comple...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-085
更新日期：2005-07-01 00:00:00
