The capsular network of Klebsiella pneumoniae.

Abstract:

:Attempts at improving chemical fixation for electron-microscopic observation of the capsule of Klebsiella pneumoniae were made. The capsule was preserved by using alcian blue - lanthanum and tris-(1-aziridinyl) phosphine oxide (TAPO) - aldehyde - osmium procedures. Despite the different retention of the overall capsular material and minor variations in morphological details, in both cases the interpretation of ultrastructural patterns suggested that the capsule be composed of a meshed network of thin polysaccharide fibrils radiating from the cell wall. This organization is in keeping with all recognized chemical properties of bacterial polysaccharide capsules or, at least, does not contradict them. Moreover, an effective preservation of bacterial structures other than capsule has been obtained, mostly in specimens fixed by the TAPO-aldehyde-osmium method, a fact which gives further reliability to the technical approach used for capsule visualization.

journal_name

Can J Microbiol

authors

Cassone A,Garaci E

doi

10.1139/m77-102

subject

Has Abstract

pub_date

1977-06-01 00:00:00

pages

684-9

issue

6

eissn

0008-4166

issn

1480-3275

journal_volume

23

pub_type

杂志文章
  • Discriminant analysis of microcalorimetric data of bacterial growth.

    abstract::In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-186

    authors: Bermúdez J,López D,Sanahuja A,Viñas M,Lorén JG

    更新日期:1988-09-01 00:00:00

  • A potential role of Escherichia coli pathobionts in the pathogenesis of pediatric inflammatory bowel disease.

    abstract::Through genomic analysis of mucosa-associated Escherichia coli strains, we found a close genetic association among isolates from pediatric inflammatory bowel disease (IBD) patients. A specific E. coli pathovar, adherent-invasive E. coli (AIEC), was found in Crohn's disease (CD) adult patients - this pathovar has enhan...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-007

    authors: Schippa S,Iebba V,Totino V,Santangelo F,Lepanto M,Alessandri C,Nuti F,Viola F,Di Nardo G,Cucchiara S,Longhi C,Conte MP

    更新日期:2012-04-01 00:00:00

  • A technique for the differential isolation of hop mosaic virus and hop latent virus.

    abstract::Two rod-shaped viruses of similar length occurring in hops were isolated from a mixed infection. One (hop mosaic virus) infected Nicotiana clevelandii, produced mosaic symptoms in mosaic-sensitive hop cultivars, and did not infect Chenopodium murale. The other (hop latent virus) infected C. murale but not N. cleveland...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-168

    authors: Probasco EG,Skotland CB

    更新日期:1976-08-01 00:00:00

  • Cytosolic and membrane-bound chitinases of two mucoraceous fungi: a comparative study.

    abstract::Chitinases isolated from membrane and cytosolic fractions of two mucoraceous fungi, Choanephora cucurbitarum and Phascolomyces articulosus, were investigated. The membrane-bound chitinase was isolated by Bio-Gel P-100 and DEAE Bio-Gel A chromatographic techniques. On SDS-PAGE the chitinase from both fungi migrated as ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-056

    authors: Balasubramanian R,Manocha MS

    更新日期:1992-04-01 00:00:00

  • Chemicals which promote survival of ultraviolet-irradiated lon and ruv mutants of Escherichia coli K12.

    abstract::The effects of postirradiation incubation with various chemicals structurally related to pantolactone on the survival of ultraviolet-irradiated Escherichia coli lon and ruv mutants were examined. Certain cyclic compounds with a butyrolactone, tetrahydrofuran, furan, tetrahydropyran, or 2-pyron ring showed substantial ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-254

    authors: Kato Y,Shiraki S,Hamada Y,Iyehara-Ogawa H,Nakayama H

    更新日期:1980-12-01 00:00:00

  • Overexpression of the dctA gene in Rhizobium meliloti: effect on transport of C4 dicarboxylates and symbiotic nitrogen fixation.

    abstract::Symbiotic nitrogen fixation may be limited by the transport of C4 dicarboxylates into bacteroids in the nodule for use as a carbon and energy source. In an attempt to increase dicarboxylate transport, a plasmid was constructed in which the Rhizobium meliloti structural transport gene dctA was fused to a tryptophan ope...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-091

    authors: Rastogi V,Labes M,Finan T,Watson R

    更新日期:1992-06-01 00:00:00

  • Biodegradation of carbazole by Ralstonia sp. RJGII.123 isolated from a hydrocarbon contaminated soil.

    abstract::The use of microorganisms for bioremediation of contaminated soils may be enhanced with an understanding of the pathways involved in their degradation of hazardous compounds. Ralstonia sp. strain RJGII.123 was isolated from soil located at a former coal gasification plant, based on its ability to mineralize carbazole,...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w99-131

    authors: Schneider J,Grosser RJ,Jayasimhulu K,Xue W,Kinkle B,Warshawsky D

    更新日期:2000-03-01 00:00:00

  • Changes in pattern of respiration and glucose utilisation in Candida utilis during the cell cycle: some variations with growth rate.

    abstract::The release of 14CO2 from 14C-labelled glucose(G-1(-14)C, G-3,4(-14)C, G-6(-14)C) was followed in phased cultures of Candida utilis grown in a glucose- mineral salts medium under altered conditions of carbon:nitrogen limitation at doubling times of 2,4 and 6, h. Changes in oxygen uptake and CO2 evolution were observe...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-150

    authors: Dawson PS,Westlake DW

    更新日期:1975-07-01 00:00:00

  • Characteristics of a nitropropanol-metabolizing bacterium isolated from the rumen.

    abstract::We report some characteristics of a ruminal bacterium (strain NPOH1) that metabolizes 3-nitropropanol, the toxic principle of various milk vetchs that are distributed worldwide. The gram-positive bacterium was nonmotile and did not produce spores. Growth of strain NPOH1 occurred under anaerobic conditions and was supp...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m97-088

    authors: Anderson RC,Rasmussen MA,DiSpirito AA,Allison MJ

    更新日期:1997-07-01 00:00:00

  • Plasmid specification of resistance to antibacterial compounds in environmental Citrobacter freundii.

    abstract::Citrobacter freundii from an environmental source displayed resistance to a wide variety of antibacterials. Some of these resistances could be transferred by conjugation with frequencies as high as 10(-2). The complexity of plasmid involvement in the antibacterial resistance of this species was demonstrated by isolati...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m81-052

    authors: Austen RA,Trust TJ

    更新日期:1981-03-01 00:00:00

  • Expression of ptxR and its effect on toxA and regA expression during the growth cycle of Pseudomonas aeruginosa strain PAO1.

    abstract::The expression of the toxA and regA genes in Pseudomonas aeruginosa is negatively regulated by iron at the transcriptional level. We have previously described ptxR, an exotoxin A regulatory gene which appears to enhance toxA expression through regA. In this study, we have tried to determine if ptxR expression correlat...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Colmer JA,Hamood AN

    更新日期:1999-12-01 00:00:00

  • Decrease in fungal biodiversity along an available phosphorous gradient in arable Andosol soils in Japan.

    abstract::Andosols comprise one of the most important soil groups for agricultural activities in Japan because they cover about 46.5% of arable upland fields. In this soil group, available phosphorus (P) is accumulated by application of excessive fertilizer, but little is known about the influence of increasing P availability o...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2012-0612

    authors: Bao Z,Matsushita Y,Morimoto S,Hoshino YT,Suzuki C,Nagaoka K,Takenaka M,Murakami H,Kuroyanagi Y,Urashima Y,Sekiguchi H,Kushida A,Toyota K,Saito M,Tsushima S

    更新日期:2013-06-01 00:00:00

  • Isolation and taxonomic and catabolic characterization of a 3,6-dimethylphenanthrene-utilizing strain of Sphingomonas sp.

    abstract::A bacterial strain capable of utilizing 3,6-dimethylphenanthrene (3,6-DMP) as its sole source of carbon and energy was isolated from a creosote-contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS1. Utilization of 3,6-DMP was demonstrated by an increase in bacteria...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-018

    authors: Sabaté J,Viñas M,Bayona JM,Solanas AM

    更新日期:2003-02-01 00:00:00

  • A technique for preserving aerial fungal structures for scanning electron microscopy.

    abstract::A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-106

    authors: King EJ,Brown MF

    更新日期:1983-06-01 00:00:00

  • Suitability of membrane-filter techniques to study the ultrastructure of Fusarium solani in soil.

    abstract::The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-239

    authors: Van Eck WH

    更新日期:1976-11-01 00:00:00

  • Physical, chemical, and microbiological characteristics of microbial mats (kopara) in the South Pacific atolls of French Polynesia.

    abstract::Microbial mats that develop in shallow brackish and hyposaline ponds in the rims of two French polynesian atolls (Rangiroa and Tetiaroa) were intensively investigated during the past three years. Comparative assessment of these mats (called kopara in polynesian language) showed remarkable similarities in their composi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Che LM,Andréfouët S,Bothorel V,Guezennec M,Rougeaux H,Guezennec J,Deslandes E,Trichet J,Matheron R,Le Campion T,Payri C,Caumette P

    更新日期:2001-11-01 00:00:00

  • The effects of pig manure application on the spread of tetracycline resistance in bulk and cucumber rhizosphere soils: a greenhouse experiment.

    abstract::It is important to understand the dynamics of tetracycline-resistant bacteria (TRB) and tetracycline resistance genes (TRGs) in bulk and rhizosphere soils for evaluating the spread of TRGs from pig manure to human. In this work, a greenhouse experiment was conducted to investigate the difference in abundance of TRB, t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2016-0767

    authors: Kang Y,Hao Y,Xia D,Shen M,Li Q,Hu J

    更新日期:2017-07-01 00:00:00

  • Effects of iron binding agents on Saccharomyces cerevisiae growth and cytochrome P450 content.

    abstract::The yeast Saccharomyces cerevisiae Y222 was studied in the presence of the following iron-binding agents: Desferal, dipyridyl, and human and bovine transferrins. We report that cell growth and lanosterol 14 alpha-demethylase cytochrome P450 are not affected by Desferal but that dipyridyl and serum transferrins decreas...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-156

    authors: Wright GD,Honek JF

    更新日期:1989-10-01 00:00:00

  • A single-bottle blood culture system: evaluation and comparison with two other systems.

    abstract::A commercially available single-bottle blood culture system was evaluated at Ben Taub General Hospital, a Harris County District Hospital. Blood cultures from 1010 patients were examined with the Lederle Diagnostics one-bottle blood culture medium-SPS, Columbia broth (E-Vac, Pfizer), and an in-house-prepared brain hea...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m77-155

    authors: Qadri SM,Wende RD

    更新日期:1977-08-01 00:00:00

  • A morphological study of anaerobic bacteria from the hypolimnia of two Michigan lakes.

    abstract::Dense populations of anaerobic bacteria were found sequentially layered below the thermocline in two eutrophic lakes in southwest Michigan. Phase and electron microscopy of whole cells and thin sections were used to reveal the in situ morphology of the dominant members of the community. The predominant chlorophyll-con...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-051

    authors: Caldwell DE,Tiedje JM

    更新日期:1975-03-01 00:00:00

  • Effects of culture conditions on yield of Shiga-like toxin-IIv from Escherichia coli.

    abstract::The effects of selected culture conditions on production of Shiga-like toxin-II variant by an edema disease strain of Escherichia coli (412) and E. coli TB1 (pCG6) containing the cloned genes for Shiga-like toxin-II variant were examined. Incubation time, culture media, incubation temperature, starting pH of the cultu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-100

    authors: MacLeod DL,Gyles CL

    更新日期:1989-06-01 00:00:00

  • DNA relatedness among aquatic yeasts of the genus Metschnikowia and proposal of the species Metschnikowia australis comb. nov.

    abstract::DNA hybridization studies were conducted to determine the taxonomic status of the aquatic group of Metschnikowia species and their varieties. Among the DNAs of the four varieties of Metschnikowia bicuspidata, that of Metschnikowia bicuspidata var. australis showed 37 to 51% relative binding with the DNAs of Metschniko...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m85-170

    authors: Mendonça-Hagler LC,Hagler AN,Phaff HJ,Tredick J

    更新日期:1985-10-01 00:00:00

  • Antifungal susceptibility and genotypical pattern of Microsporum canis strains.

    abstract::Dermatophytes are a group of fungi that are capable of invading keratinized tissues of humans and other animals. Antifungal susceptibility analysis and genetic studies by random amplification of polymorphic DNA (RAPD), have been used to detect polymorphism as well as determining the possible resistance of dermatophyte...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-024

    authors: Brilhante RS,Cordeiro RA,Medrano DJ,Monteiro AJ,Sidrim JJ,Rocha MF

    更新日期:2005-06-01 00:00:00

  • Measurement of dissolved carbon dioxide.

    abstract::Several probes for measuring dissolved carbon dioxide (CO2) concentration were installed in a 68-litre fermentor and their effectiveness compared. Submerged silastic rubber tubing gave reproducible results over a wide range of operating conditions and was generally superior to all other probes evaluated. The silastic ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-007

    authors: Alford JS Jr

    更新日期:1976-01-01 00:00:00

  • Cell wall proteins of Candida albicans.

    abstract::Proteins were solubilized from cell wall fractions of Candida albicans and separated by polyacrylamide gel electrophoresis. Cell walls were isolated from 25 and 37 degrees C growing and stationary phase yeast cultures and from germ tubes. The 42 protein bands detected by dye binding were observed in all wall extracts,...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-220

    authors: Chaffin WL,Stocco DM

    更新日期:1983-10-01 00:00:00

  • [Effect of monensin on the growth and secretion of exopolysaccharides in Botrytis cinerea Pers. and Sclerotium rolfsii Sacc].

    abstract::The addition of various concentrations of monensin (1,5, and 10 micrograms/mL) to the culture medium inhibits the fungal growth and perturbs exopolysaccharides secretion, provoking a decrease of production in Botrytis cinerea and an increase in Sclerotium rolfsii. The ionophore induces also modifications in both polym...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-124

    authors: Koulali Y,Talouizte A,Fonvieille JL,Dargent R

    更新日期:1996-09-01 00:00:00

  • Staphylococcus aureus strains differ in their in vitro responsiveness to human urokinase: evidence that methicillin-resistant strains are predominately nonresponsive to the growth-enhancing effects of urokinase.

    abstract::Clinical isolates of Staphylococcus aureus were found to exhibit strain-specific heterogeneity to the growth-enhancing effects of human urokinase (UK), a proteinase with plasminogen activator activity. Nine out of fourteen (64%) methicillin-sensitive strains of S. aureus were responsive to UK in "in vitro" cultures. I...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-131

    authors: Hart DA,Louie T,Krulicki W,Reno C

    更新日期:1996-10-01 00:00:00

  • Hydroxylase regulation in Candida tropicalis grown on alkanes.

    abstract::Candida tropicalis synthesizes a hydroxylase (3 to 5 nmol of product formed per minute per milligram of protein) and a cytochrome P-450 (0.10 to 0.13 nmol per milligram of protein) during growth on n-tetradecane. A three- to four-fold increase in the level of NADPH cytochrome c reductase is also observed in those cell...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-031

    authors: Gilewicz M,Zacek M,Bertrand JC,Azoulay E

    更新日期:1979-02-01 00:00:00

  • Factors influencing potent antagonistic effects of Escherichia coli and Bacteroides ovatus on Staphylococcus aureus in anaerobic continuous flow cultures.

    abstract::We examined factors related to the potent antagonistic effect of Escherichia coli and Bacteroides ovatus on Staphylococcus aureus in anaerobic continuous flow cultures. In the presence of sugars fermentable by E. coli alone or both E. coli and S. aureus, motile E. coli strains exerted a potent antagonistic effect and ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-106

    authors: Ushijima T,Ozaki Y

    更新日期:1988-05-01 00:00:00

  • Directed biosynthesis of novel derivatives of echinomycin. II. Purification and structure elucidation.

    abstract::New antibiotics produced by Streptomyces echinatus A8331 cultured in the presence of heterocyclic aromatic acids can be separated and purified by high-performance liquid chromatography using reversed phase columns. Natural quinoxaline antibiotics and certain quinoline derivatives can also be efficiently separated in n...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m84-112

    authors: Gauvreau D,Waring MJ

    更新日期:1984-06-01 00:00:00