Abstract:
:A bacterial strain capable of utilizing 3,6-dimethylphenanthrene (3,6-DMP) as its sole source of carbon and energy was isolated from a creosote-contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS1. Utilization of 3,6-DMP was demonstrated by an increase in bacterial biomass concomitant with a decrease in 3,6-DMP in a liquid mineral medium with this compound as its sole source of carbon and energy. Strain JS1 showed a high specificity in the use of the most abundant alkylderivatives of crude oils, such as alkylnaphthalenes and other alkylphenanthrenes, as the sole source of carbon and energy. It can also use several polycyclic aromatic hydrocarbons of three and four rings and their alkylated derivatives as growth substrates or transform them. The identification of several intermediate metabolites points to extensive metabolic activity, including the following: (i) aromatic ring oxidation and cleavage, (ii) methyl group oxidations, and (iii) methylenic oxidations. The metabolic actions of Sphingomonas sp. JS1 on the aromatic fraction extracted from a creosote-contaminated soil are also examined.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Sabaté J,Viñas M,Bayona JM,Solanas AMdoi
10.1139/w03-018subject
Has Abstractpub_date
2003-02-01 00:00:00pages
120-9issue
2eissn
0008-4166issn
1480-3275pii
w03-018journal_volume
49pub_type
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