Stress-survival responses of a carbon-starved p-nitrophenol-mineralizing Moraxella strain in river water.

Abstract:

:The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and 2-day carbon-starved Moraxella cultures survived about 150-, 200-, and 100-fold better than the non-starved cultures when exposed to 43.5 degrees C, 2.7 mol/L NaCl, and 500 micromol/L H2O2 for 4 h, respectively. A green fluorescent protein gene- (gfp) labelled derivative of the Moraxella strain was used to examine the stress-resistant responses of the bacterium in natural river water microcosms. The carbon-starved gfp-labelled Moraxella strain also showed stress-resistant responses against heat, osmotic, and oxidative stresses in the river water samples. Despite the stress-tolerant capability of the carbon-starved gfp-labelled Moraxella cells, they did not exhibit any survival advantage over their non-starved counterparts when inoculated into river water microcosms and incubated at 10 and 22 degrees C for 14 d.

journal_name

Can J Microbiol

authors

Moore M,Trevors J,Lee H,Leung KT

doi

10.1139/w04-131

subject

Has Abstract

pub_date

2005-03-01 00:00:00

pages

223-9

issue

3

eissn

0008-4166

issn

1480-3275

pii

w04-131

journal_volume

51

pub_type

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