Abstract：
:Roots of field-grown tropical maize, Panicum maximum Jacq. and Digitaria decumbens Stent., and of sorghum and wheat grown in monoxenic culture with the diazotroph Spirillum lipoferum (syn. Azospirillum spp.) were examined for tetrazolium-reducing bacteria following incubation of roots in a malate-phosphate buffer-2,3,5-triphenyltetrazolium chloride medium. Bacteria were observed between and in cells of the cortex, in intercellular spaces between the cortex and endodermis, in xylem cells, and in and between pith cells. In maize, colonization of the inner cortex and stele appears to occur in the absence of significant bacterial colonization or collapse of outerlying tissues. Bacteria in the stele remained viable after a 6-h treatment of roots with chloramine-t, indicating that the endodermis was intact. Infection of the inner cortex and stele appears to occur initially in branches, and then to spread longitudinally into main roots. Inter- and intra-cellular infections of the cortex were observed in monoxenic systems. Tetrazolium reduction and prominent crystal formation was not specific for diazotrophic bacteria, but S. lipoferum was isolated from surface-sterilized roots, and S. lipoferum-like organisms were observed in the endorhizosphere. A correlation of inner cortex and stele infections with the presence of branches appears to explain previous observations that excised roots of grasses exhibiting high nitrogenase activity are characteristically branched roots with an intact cortex.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Patriquin DG,Döbereiner Jdoi
10.1139/m78-122subject
Has Abstractpub_date
1978-06-01 00:00:00pages
734-42issue
6eissn
0008-4166issn
1480-3275journal_volume
24pub_type
杂志文章abstract：:Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0206
更新日期：2014-08-01 00:00:00
abstract：:The aim of this study was to assess the influence of sodium content on the microbiota on the surface of ready-to-eat (RTE) meat products purchased from the retail market in Canada. Products, including sliced and sausage-type deli meats, were analysed with culture-dependent and culture-independent methods. Bacteria wer...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0630
更新日期：2015-02-01 00:00:00
abstract：:Wild-type glucose uptake and glucose chemotaxis activities were restored in glucose transport defective Pseudomonas aeruginosa strains PFB360 and PFB362 after introduction of plasmid pPZ129, containing a 1.1-kilobase DNA fragment that is essential for the expression of the P. aeruginosa periplasmic glucose binding pro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-163
更新日期：1993-11-01 00:00:00
abstract：:Strain-specific fluorescent antibodies were prepared for three isolates of Aereomonas hydrophila. These antisera were reacted with 255 other A. hydrophila isolates, and their immunofluorescence was measured quantitatively by photometric fluorescence microscopy. Only 27.5% of the isolates reacted with the prepared anti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-024
更新日期：1980-02-01 00:00:00
abstract：:Cellobiose transport by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes was measured using randomly tritiated cellobiose. When assayed at the same concentration (1 mM), total cellobiose uptake was one-fourth to one-third that of total glucose uptake. The abilities of F. succinogenes to transpo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-021
更新日期：1991-02-01 00:00:00
abstract：:The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cyto...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0606
更新日期：2013-06-01 00:00:00
abstract：:Fungal cultures were grown by a new microslide method and by the traditional slide-culture method. Sporulation results were similar by both methods. The microslide method has the advantages of increased safety to the user and ease of storage. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-204
更新日期：1978-10-01 00:00:00
abstract：:Rhizobia are used exclusively in agricultural systems for enhancing the ability of legumes to fix atmospheric nitrogen. Knowledge about the indigenous population is necessary for the selection and application of inoculant strains. In this study, we have assessed the genetic diversity of Bradyrhizobium strains isolated...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-139
更新日期：2001-02-01 00:00:00
abstract：:A nystatin-resistant mutant of Aspergillus niger is described that has quantitative changes in phospholipid composition compared with the wild type. The proportion of linoleic acid in total phospholipids was elevated in the mutant from 38 to 56% and the proportion of oleic acid was lowered from 28 to 12%. Amino acid t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-076
更新日期：1990-06-01 00:00:00
abstract：:Responses of Rhizoctonia solani to herbicides in soil cultures were assessed by measuring soil enzyme activity and other growth-related factors. Both beta-galactosidase (EC 3.2.1.23) and phosphatase (EC 3.1.3.1.3.1.3.2) activities were highly correlated with amounts of mycelium in soil. Both enzyme activities were red...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-089
更新日期：1977-05-01 00:00:00
abstract：:Pleomorphic deterioration is a process where a fungal isolate loses the ability to produce conidia during repeated subculturing. We have previously isolated strains of the entomopathogenic fungus Metarhizium anisopliae that have irreversibly lost the ability to produce conidia and only produce mycelia when grown on ag...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-074
更新日期：2002-09-01 00:00:00
abstract：:A test is described for selective inhibition of Mycobacterium tuberculosis and M. bovis isolates in fluid medium. The method employs rho-nitro-alpha-acetyl-amino-beta-hydroxy-propiophenone (NAP) as an inhibitory agent for differentiation of mammalian tuberculosis strains from other Mycobacteria. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-124
更新日期：1978-06-01 00:00:00
abstract：:A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-059
更新日期：1997-05-01 00:00:00
abstract：:The goal of this study was to evaluate the antibiofilm and antimicrobial activities of Bacoside A, a formulation of phytochemicals from Bacopa monnieri, against Staphylococcus aureus and Pseudomonas aeruginosa, which are known to form biofilms as one of their virulence traits. The antimicrobial effects of Bacoside A w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0365
更新日期：2017-02-01 00:00:00
abstract：:Bacterial biofilms are commonly formed on medical devices and food processing surfaces. The antimicrobials used have limited efficacy against the biofilms; therefore, new strategies to prevent and remove these structures are needed. Here, the effectiveness of brief oxidative treatments, based on the combination of sod...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0747
更新日期：2015-05-01 00:00:00
abstract：:Approximately 20% of Canada's oil supply now comes from the extraction of bitumen from the oil sands deposits in northeastern Alberta. The oil sands are strip-mined, and the bitumen is typically separated from sand and clays by an alkaline hot water extraction process. The rapidly expanding oil sands industry has mill...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-129
更新日期：2002-01-01 00:00:00
abstract：:Subtyping Neisseria meningitidis by methods that rely on monoclonal antibody (mAb) reactivity results in an unusually high number of strains that are not subtypeable. To subtype 48 strains isolated (1993-1994) in the province of Quebec that were not subtypeable by mAb-based techniques, we used DNA sequencing of the va...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:3-O-Methyl-D-glucose (3-O-MeGlc) or a mixture of 3-O-MeGlc and glucose stimulate invertase, beta-glucosidase, alpha-glucosidase, and alpha-galactosidase production by Cryptococcus laurentii. They also increase invertase and alpha-glucosidase production by Saccharomyces cerevisiae. The stimulatory effect of 3-O-MeGlc i...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-215
更新日期：1980-11-01 00:00:00
abstract：:Absorption of 14C-labelled poliovirus-2 to sedimentable solids of primary sludge samples collected from a secondary treatment facility during a 6-month period averaged 94%; for anaerobically digested sludge, 99%. The extent of virus adsorption was influenced by the amount of solids. Maximal adsorption occurred at or a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-254
更新日期：1983-12-01 00:00:00
abstract：:Marine shrimp of the species Penaeus monodon were collected from the coastal region (Haroa) of the deltaic Sundarbans of West Bengal, India during the premonsoon period in 1996. Klebsiella pneumoniae was isolated from the alimentary canal and gills of the shrimp as the sole isolate. All 10 isolated strains were resist...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:An alginate lyase gene of a newly isolated Pseudomonas sp. strain KS-408 was cloned by using PCR with the specific primers designed from homologous nucleotide sequences. A partial protein sequence of KS-408 alginate lyase was homology-modeled on the basis of the crystal structure of A1-III alginate lyase from Sphingom...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-106
更新日期：2011-12-01 00:00:00
abstract：:Of 33 streptococcal cultures belonging to serological group L, all bound human immunoglobulin (Ig) G, fibrinogen, and fibronectin; 32 bound bovine IgG; 31 bound alpha 2-macroglobulin; 5 bound albumin; and none bound either haptoglobin or IgA. The binding sites for IgG could be isolated from the L streptococci by tryps...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-001
更新日期：1988-01-01 00:00:00
abstract：:The adherence of Streptococcus pneumoniae to epithelial (A549) lung cells was studied and the bacterial binding distribution was found to be nonrandom (non-Gaussian). Analysis of the dependency of bacterial binding on the cell cycle of A549 cells revealed that approximately 1.8 times more bacteria bind to G2 cells tha...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:Urease activity was measured using whole cells of both long (swarming) and short (nonswarming) populations of Proteus mirabilis from casein hydrolysate agar (CHA) and broth (CHB) cultures, and from brain heart infusion broth (BHIB) cultures. Urease is a constitutive enzyme for both long and short cells, but its activi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-051
更新日期：1987-04-01 00:00:00
abstract：:Seven streptomycete strains were tested for biotransformation of salicylate. The products were identified by nuclear magnetic resonance spectroscopy and three types of conversion were found. Streptomyces cinnamonensis and Streptomyces spectabilis formed gentisate and salicylamide concurrently. Streptomyces rimosus tra...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-111
更新日期：1996-08-01 00:00:00
abstract：:Cell surface hydrophobicity influences adhesion and virulence of the opportunistic fungal pathogen Candida albicans. Previous studies have shown that cell surface hydrophobicity is due to specific proteins that are exposed on hydrophobic cells but are masked by long fibrils on hydrophilic cells. This observation sugge...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-043
更新日期：1994-04-01 00:00:00
abstract：:Despite the interactions known to occur between various lower respiratory tract pathogens and alveolar epithelial cells (AECs), few reports examine factors influencing the interplay between Mycobacterium tuberculosis bacilli and AECs during infection. Importantly, in vitro studies have demonstrated that the M. tubercu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0528
更新日期：2015-12-01 00:00:00
abstract：:The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0770
更新日期：2016-07-01 00:00:00
abstract：:Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteri...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0343
更新日期：2020-10-01 00:00:00
abstract：:beta-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-146
更新日期：2009-04-01 00:00:00
