Abstract：
:Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolomics in combination with multivariate data analysis methods to comprehensively analyze the metabolic changes in nicotine-treated Escherichia coli. Our results showed that nicotine caused the changes of energy-related metabolism that we believe are due to enhanced glycolysis and mixed acid fermentation as well as inhibited tricarboxylic acid cycle activity. Furthermore, nicotine resulted in the alteration of choline metabolism with a decreased synthesis of betaine but an increased production of dimethylamine. Moreover, nicotine caused a decrease in amino acid concentration and an alteration of nucleotide synthesis. We hypothesize that these changes caused the decrease in bacterial cell density observed in the experiment. These findings provide a comprehensive insight into the metabolic response of E. coli to nicotine stress. Our study highlights the value of metabolomics in elucidating the metabolic mechanisms of nicotine action.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Ding L,Chen J,Zou J,Zhang L,Ye Ydoi
10.1139/cjm-2014-0206subject
Has Abstractpub_date
2014-08-01 00:00:00pages
547-56issue
8eissn
0008-4166issn
1480-3275journal_volume
60pub_type
杂志文章abstract：:Environmental factors influencing the occurrence and community structure of soil yeasts in forests are not well studied. There are few studies dedicated to Southern Hemisphere soil yeasts populations and even fewer focused on temperate forests influenced by volcanic activity. The present work aimed to study the ecolog...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0897
更新日期：2014-06-01 00:00:00
abstract：:Evidence has shown that individuals exposed to indoor toxic molds for extended periods of time have elevated risk of developing numerous respiratory illnesses. It is not clear at the cellular level what impact mold exposure has on the immune system. Herein, we show that 2 fungal volatiles (E)-2-octenal and oct-1-en-3-...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0708
更新日期：2014-01-01 00:00:00
abstract：:The marine sponge Mycale sp., a potential source of natural bioactive products, is widely distributed along the coast of Fujian, China. The cultivable bacterial community associated with Mycale sp., the antibacterial activities, and the PKS (polyketide synthase) and NRPS (nonribosomal peptide synthetase) gene diversit...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0785
更新日期：2014-04-01 00:00:00
abstract：:The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-120
更新日期：1979-07-01 00:00:00
abstract：:The effects of seven thiophosphorus pesticides and their degradation products on estuarine ammonium oxidizers were examined. Using an axenic, closed-culture, high cell density assay, the pesticides caused little inhibition of ammonium oxidation. However, the degradation product of methyl parathion, p-aminophenol, sign...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-216
更新日期：1980-11-01 00:00:00
abstract：:Our freshwater caulobacter collection contains about 40 strains that are morphologically similar to Caulobacter crescentus. All elaborate a crystalline protein surface (S) layer made up of protein monomers 100-193 kDa in size. We conducted a comparative study of S-layer secretion in 6 strains representing 3 size group...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-046
更新日期：2004-09-01 00:00:00
abstract：:In the past decade, the large tailings pond (Mildred Lake Settling Basin) on the Syncrude Canada Ltd. lease near Fort McMurray, Alta., has gone methanogenic. Currently, about 60%-80% of the flux of gas across the surface of the tailings pond is methane. As well as adding to greenhouse gas emissions, the production of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:Schizophyllum commune Fr. was shown, by light, scanning, and transmission electron microscopy, to be a destructive mycoparasite on several phytopathogenic and nematode-trapping fungi. The hyphae of S. commune coiled around host hyphae and fruiting structures and penetrated them by means of either unspecialized hyphae ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-131
更新日期：1978-07-01 00:00:00
abstract：:A stable enrichment culture converting acetic acid to methane was successfully obtained from a pear waste digester, using a synthetic substrate solution with acetic acid as the main carbon source. This enrichment culture converted up to 10 mmol of acetic acid per litre per day at 35 degrees C and did not use hydrogen ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-194
更新日期：1976-09-01 00:00:00
abstract：:Unicellular green alga represents not only a convenient model for its biochemical and physiological studies but also a sensitive system to test the effects of various environmental factors. Algae cells of two strains, SA-3 strain (exsymbiotic from Paramecium bursaria) and Chlorella vulgaris c-27, were asynchronously c...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-143
更新日期：2002-02-01 00:00:00
abstract：:An endo-beta-1,4-xylanase gene was cloned from Neocallimastix patriciarum 27 in the bacteriophage vector lambda gtWES lambda B and was subcloned into the plasmid vectors pUC18 and pUC19 in which xylanase activity was expressed in both orientations. The xylanase was located in the periplasmic space of the host, Escheri...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-020
更新日期：1993-01-01 00:00:00
abstract：:The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation pat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-090
更新日期：1990-07-01 00:00:00
abstract：:The Bacillus subtilis glutamyl-tRNA synthetase (GluRS), encoded by the gltX gene, aminoacylates its homologous tRNA(Glu) and tRNA(Gln) with glutamate. This gene was cloned with its sigma A promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and B. s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:Viruses were isolated from every sample of raw (100 L) and treated (1000 L) water collected at a water treatment plant drawing sewage-contaminated river water. Few plaque-forming isolates were formed but cytopathogenic viruses were isolated as frequently in drinking water as in raw water. In drinking water some sample...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-063
更新日期：1981-04-01 00:00:00
abstract：:Human astrovirus (HAstV) was an important cause of viral gastroenteritis in infants in Wuhan city based on our previous study. The aim of the study was to investigate the nature of HAstV infection in Wuhan, People's Republic of China, especially in adults. Stool specimens were collected from 361 children and 301 adult...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-085
更新日期：2011-11-01 00:00:00
abstract：:Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-148
更新日期：1976-07-01 00:00:00
abstract：:The cellular functions are strongly influenced by the composition of the environment. In particular, phenotypes of microbial strains are modulated by concentrations of ions in the culture medium, and differences in element levels may be responsible for a phenotypic variability observed when microbial strains are grown...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-012
更新日期：2007-04-01 00:00:00
abstract：:In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-186
更新日期：1988-09-01 00:00:00
abstract：:Scanning and transmission electron microscopy was applied for a morphological study of three strains of Bifidobacterium grown on solid or liquid media. The pronounced pleomorphism of the cultures previously observed by light microscopy was confirmed. A possible sequence of the morphological events during transformatio...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-197
更新日期：1975-09-01 00:00:00
abstract：:A Gram-negative aerobic bacterium, designated as JN8, was isolated from activated sludge and soil in a pesticides factory in China. It was found that JN8 had a high capacity for degrading a broad range of type II pyrethroids and utilizing these pyrethroids as the sole carbon source for cell growth. The degradation rat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0104
更新日期：2014-08-01 00:00:00
abstract：:One of the important properties of probiotics is the ability to survive in the intestine. There have been few studies on the probiotic property of lactococci, since they are formally not considered to be natural inhabitants of the intestine. To evaluate lactococci as probiotic bacteria, we investigated their ability t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-092
更新日期：2003-11-01 00:00:00
abstract：:The microbial community plays an important role in soil nutrient cycles and energy transformations in alpine grassland. In this study, we investigated the composition of the soil microbial community collected from alpine cold swamp meadow (ASM), alpine cold meadow (AM), and alpine cold desert steppe (ADS) within the B...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2017-0585
更新日期：2018-03-01 00:00:00
abstract：:We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-126
更新日期：1992-08-01 00:00:00
abstract：:Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to detect proteins induced in Frankia sp. strain ACN14a-tsr by root exudates of its symbiotic host, Alnus glutinosa. The 5 most prominent proteins were purified from 2-D gels and characterized by N-terminal sequencing. All of these proteins had a high p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-046
更新日期：2001-06-01 00:00:00
abstract：:Candida albicans biofilm formation is considered an important matter because it can lead to strong resistance to conventional antifungal agents. Hyphae formed by C. albicans can also act as an important virulence factor related to its biofilm. The objective of this study was to determine the effect of zerumbone, a mon...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0155
更新日期：2019-10-01 00:00:00
abstract：:The influence of plant growth promoting (PGP) activity of bacterial communities recovered from each of six weed species (barnyard grass (Echinochloa crusfalli (L.) Beauv.), corn spurrey (Spergula arvensis L.), goldenrod (Sonchus sp.), Italian ryegrass (Lolium multiflorum L.), lamb's-quarters (Chenopodium album L.), an...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-110
更新日期：2001-11-01 00:00:00
abstract：:Methanol extracts were obtained from L. interrogans serovars icterohaemorrhagiae and canicola and L. biflexa serovar patoc. Human sera from 167 normal individuals and 40 patients with different infectious diseases tested by complement fixation tests showed negative reactions. Sera from 100 patients with a suspicion of...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-075
更新日期：1991-06-01 00:00:00
abstract：:The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0770
更新日期：2016-07-01 00:00:00
abstract：:Growth of Neisseria perflava, Neisseria cinerea, and Neisseria sicca strain Kirkland in media supplemented with sucrose (0.5 to 5.0% w/v) resulted in the formation of giant cells. Response to sucrose was specific in that a variety of other carbohydrates did not mediate giant cell formation. Giant cells appeared only u...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-065
更新日期：1976-03-01 00:00:00
abstract：:Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. A...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0763
更新日期：2015-03-01 00:00:00
