The effects of fungal volatile organic compounds on bone marrow stromal cells.

Abstract:

:Evidence has shown that individuals exposed to indoor toxic molds for extended periods of time have elevated risk of developing numerous respiratory illnesses. It is not clear at the cellular level what impact mold exposure has on the immune system. Herein, we show that 2 fungal volatiles (E)-2-octenal and oct-1-en-3-ol have cytotoxic effects on murine bone marrow stromal cells. To further analyze alterations to the cell, we evaluated the impact these volatile organic compounds have on membrane composition and hence fluidity. Both (E)-2-octenal and oct-1-en-3-ol exposure caused a shift to unsaturated fatty acids and lower cholesterol levels in the membrane. This indicates that the volatile organic compounds under investigation increased membrane fluidity. These vast changes to the cell membrane are known to contribute to the breakdown of normal cell function and possibly lead to death. Since bone marrow stromal cells are vital for the appropriate development and activation of immune cells, this study provides the foundation for understanding the mechanism at a cellular level for how mold exposure can lead to immune-related disease conditions.

journal_name

Can J Microbiol

authors

Hokeness K,Kratch J,Nadolny C,Aicardi K,Reid CW

doi

10.1139/cjm-2013-0708

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

1-4

issue

1

eissn

0008-4166

issn

1480-3275

journal_volume

60

pub_type

杂志文章
  • Prevention of 5-fluorouracil-induced infection with indigenous Escherichia coli in tumor-bearing mice by nonspecific immunostimulation.

    abstract::We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-126

    authors: Nomoto K,Yokokura T,Nomoto K

    更新日期:1992-08-01 00:00:00

  • Uneven genotypic diversity of Escherichia coli in fecal sources limits the performance of a library-dependent method of microbial source tracking on the southwestern French Atlantic coast.

    abstract::To develop a library-dependent method of tracking fecal sources of contamination of beaches on the Atlantic coast of southwestern France, a library of 6368 Escherichia coli isolates was constructed from samples of feces, from 40 known human or animal sources collected in the vicinity of Arcachon Bay in 2010, and in Fr...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2020-0244

    authors: Garabetian F,Vitte I,Sabourin A,Moussard H,Jouanillou A,Mornet L,Lesne M,Lyautey E

    更新日期:2020-12-01 00:00:00

  • Diversity of antifungal actinomycetes in various vegetative soils of Korea.

    abstract::Diversity of actinomycetes and their antifungal activities against some plant pathogenic fungi were examined in various vegetative soils from 14 different sites in the western part of Korea. Actinomycete counts ranged from 1.17 x 10(6) to 4.20 x 10(6) cfu x g(-1) dried soil. A total of 1510 actinomycetes were isolated...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w02-025

    authors: Lee JY,Hwang BK

    更新日期:2002-05-01 00:00:00

  • Inhibition of uridine 5'-diphosphate-N-acetylmuramyl-L-alanine synthetase by beta-chloro-L-alanine in Escherichia coli.

    abstract::The synthesis of the nucleotide precursors for peptidoglycan is regulated by the relA gene in Escherichia coli. Thus, nucleotide precursors labeled with [3H]diaminopimelic acid accumulated in a relA strain but not in an isogenic relA+ strain during amino acid deprivation. Furthermore, nucleotide precursor synthesis wa...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m82-098

    authors: Ishiguro EE

    更新日期:1982-06-01 00:00:00

  • Genomic comparison of facultatively anaerobic and obligatory aerobic Caldibacillus debilis strains GB1 and Tf helps explain physiological differences.

    abstract::Caldibacillus debilis strains GB1 and Tf display distinct phenotypes. Caldibacillus debilis GB1 is capable of anaerobic growth and can synthesize ethanol while C. debilis Tf cannot. Comparison of the GB1 and Tf genome sequences revealed that the genomes were highly similar in gene content and showed a high level of sy...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2018-0464

    authors: Wushke S,Froese A,Fristensky B,Zhang XL,Spicer V,Krokhin OV,Levin DB,Sparling R

    更新日期:2019-06-01 00:00:00

  • Characterization of 4 T1-like lytic bacteriophages that lyse Shiga-toxin Escherichia coli O157:H7.

    abstract::Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC st...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-063

    authors: Niu YD,Stanford K,Ackermann HW,McAllister TA

    更新日期:2012-07-01 00:00:00

  • Studies on experimental murine histoplasmosis: host protection and cellular immunity.

    abstract::Mice immunized subcutaneously with 10(6) viable yeast cells of Histoplasma capsulatum exhibited host protection to a challenge infection and also exhibited concomitant manifestations of cellular immunity to a culture filtrate antigen (yeast extract dialysate (YED)-histoplasmin). Protective immunity was determined by q...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-016

    authors: Burt WR,Smith RA

    更新日期:1983-01-01 00:00:00

  • Mapping of transfer and H pilus coding regions of the IncHII plasmid pHH1508a.

    abstract::The IncHII plasmid pHH1508a (208 kilobases) encodes resistance to potassium tellurite, trimethoprim, and streptomycin. Conjugative pili encoded by pHH1508a were isolated, purified, and used for preparation of anti-H pilus antiserum. Immuno-gold labelling experiments using H pilus specific antiserum showed that antigen...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-043

    authors: Yan W,Taylor DE

    更新日期:1989-02-01 00:00:00

  • Biochemical identification of a tryptophan auxotroph in Rhodospirillum rubrum.

    abstract::A tryptophan auxotroph of aerobically grown Rodospirillum rubrum was isolated after mutagenesis and replica plating. The mutant grows on tryptophan or indole, accumulates indole glycerol phosphate, lacks the capacity to convert indole glycerol phosphate to indole glycerol phosphate to indole, and finally is defective ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-055

    authors: Beuchemin-Newhouse N,Cedergen RJ

    更新日期:1978-03-01 00:00:00

  • Lethality of Candida strains as influenced by the host.

    abstract::A comparative study of the pathogenicity of Candida albicans morphological mutant strains was made to relate chlamydospore production, germ tube formation, and proteolytic activity to candidiasis in mice. It was observed that the mycelium strains were more lethal than the yeast-like strains and that neither chlamydosp...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-093

    authors: Saltarelli CG,Gentile KA,Mancuso SC

    更新日期:1975-05-01 00:00:00

  • Antioxidant, antihypertensive, and antibacterial properties of endophytic Pestalotiopsis species from medicinal plants.

    abstract::Pestalotiopsis species were most dominant endophytic species isolated from four medicinal plants including Terminalia arjuna, Terminalia chebula, Azadirachta indica, and Holarrhena antidysenterica. Thirty Pestalotiopsis species isolated from different parts of the medicinal plants were selected for the study. The anti...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-070

    authors: Tejesvi MV,Kini KR,Prakash HS,Subbiah V,Shetty HS

    更新日期:2008-09-01 00:00:00

  • Importance of the icmN gene in the growth of Legionella pneumophila in amoebic cells at low temperature.

    abstract::Legionella pneumophila grows in amoebae and has achieved the ability to grow at various temperatures, although the mechanisms controlling this ability remain poorly understood. The Icm/Dot type IVB secretion system is composed of more than 25 proteins and is known to be essential for intracellular growth. The role of...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-011

    authors: Qin T,Iida K,Piao Z,Shiota S,Ren H,Shao Z,Yoshida S

    更新日期:2012-04-01 00:00:00

  • Murine cytomegalovirus-Pseudomonas synergistic infections: comparison of virulent and attenuated virus.

    abstract::The synergistic interactions between Pseudomonas aeruginosa and virulent or attenuated murine cytomegalovirus (MCMV) were compared in vivo. Virulent MCMV challenge at a dose of 5 X 10(5) pfu/mouse intraperitoneally, followed by intranasal superinfection with 5 X 10(6) cfu/mouse of Pseudomonas aeruginosa after 48 h res...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m87-161

    authors: Kournikakis B,Babiuk LA

    更新日期:1987-10-01 00:00:00

  • BaeR protein acts as an activator of nuclear factor-kappa B and Janus kinase 2 to induce inflammation in murine cell lines.

    abstract::BaeR, a response regulator protein, takes part in multidrug efflux, bacterial virulence activity, and other biological functions. Recently, BaeR was shown to induce inflammatory responses by activating the mitogen-activated protein kinases (MAPKs). In this study, we investigated additional pathways used by BaeR to ind...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2016-0057

    authors: Lee SJ,Birhanu BT,Awji EG,Kim MH,Park JY,Suh JW,Park SC

    更新日期:2016-09-01 00:00:00

  • Restriction analysis of actinomycetes chromosomal DNA.

    abstract::Actinomycetes DNAs were digested with restriction enzymes to study the presence of methylated bases. Analysis showed that the enterobacterial Dam and Dcm systems are absent. Methylation at the internal cytosine in CCGG sequences, typical of eukaryotes, was also absent. We also tested 18 restriction endonucleases recog...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-031

    authors: Novella IS,Marín I,Sánchez J

    更新日期:1996-02-01 00:00:00

  • Biochemical characterization of three mycobacterial ribosomal fractions.

    abstract::The induction of antituberculous immunity by crude ribosomal fractions isolated from Mycobacterium tuberculosis strain H37Ra, M. bovis strain BCG, and M. smegmatis was studied in CF-1 mice. Levels of antituberculous immunity similar to that induced by live BCG were induced by the BCG and H37Ra ribosomal fractions wher...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-032

    authors: Portelance V,Beaudet R

    更新日期:1983-02-01 00:00:00

  • Subcellular localization of the Streptococcus mutans P1 protein C terminus.

    abstract::To determine the subcellular location of the Streptococcus mutans P1 protein C-terminal anchor, cell envelope fractionation experiments were conducted in combination with Western immunoblotting, using monoclonal antibody MAb 6-8C specific for an epitope that maps near the C terminus of P1 protein and also a polyclonal...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Homonylo-McGavin MK,Lee SF,Bowden GH

    更新日期:1999-06-01 00:00:00

  • Enzyme activities associated with oxidative stress in Metarhizium anisopliae during germination, mycelial growth, and conidiation and in response to near-UV irradiation.

    abstract::Metarhizium anisopliae isolates have a wide insect host range, but an impediment to their commercial use as a biocontrol agent of above-ground insects is the high susceptibility of spores to the near-UV present in solar irradiation. To understand stress responses in M. anisopliae, we initiated studies of enzymes that ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-097

    authors: Miller CD,Rangel D,Braga GU,Flint S,Kwon SI,Messias CL,Roberts DW,Anderson AJ

    更新日期:2004-01-01 00:00:00

  • The nucleotide sequence of herpes simplex virus type 2 (333) glycoprotein gB2 and analysis of predicted antigenic sites.

    abstract::The gene coding for glycoprotein B2 (gB2) of herpes simplex virus type 2 (HSV-2) strain 333 was mapped and its nucleotide sequence determined. Open reading frame analysis deduced a polypeptide consisting of 902 amino acids and having close homology to gB1 of HSV type 1. Several predicted features of gB2 are consistent...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m87-154

    authors: Zwaagstra JC,Leung WC

    更新日期:1987-10-01 00:00:00

  • Overexpression of the dctA gene in Rhizobium meliloti: effect on transport of C4 dicarboxylates and symbiotic nitrogen fixation.

    abstract::Symbiotic nitrogen fixation may be limited by the transport of C4 dicarboxylates into bacteroids in the nodule for use as a carbon and energy source. In an attempt to increase dicarboxylate transport, a plasmid was constructed in which the Rhizobium meliloti structural transport gene dctA was fused to a tryptophan ope...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-091

    authors: Rastogi V,Labes M,Finan T,Watson R

    更新日期:1992-06-01 00:00:00

  • Incidence of Norwalk virus infections during a prospective epidemiological study of drinking water-related gastrointestinal illness.

    abstract::To determine the seroprevalence of Norwalk virus and whether Norwalk virus contributed to an observed increase in illness in tap water drinkers participating in a prospective epidemiological study, sera collected during the study were examined for changes in Norwalk virus antibody titer, using a specific enzyme immuno...

    journal_title:Canadian journal of microbiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1139/m94-128

    authors: Payment P,Franco E,Fout GS

    更新日期:1994-10-01 00:00:00

  • Inactivation of human adenovirus by sequential disinfection with an alternative ultraviolet technology and monochloramine.

    abstract::In an effort to reduce human exposure to adenoviruses through drinking water, we determined the effectiveness of sequential disinfection with an alternative ultraviolet (UV) technology (medium-pressure (MP) UV) and monochloramine. The results of this study showed that MP UV was much more effective than traditional UV ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-047

    authors: Shin GA,Lee JK

    更新日期:2010-07-01 00:00:00

  • Immunological studies on staphylococcal enterotoxin D: production of murine monoclonal antibodies and immunopurification.

    abstract::Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m91-099

    authors: Shinagawa K,Omoe K,Matsusaka N,Sugii S

    更新日期:1991-08-01 00:00:00

  • Bindings of plasma proteins to streptococci of serological group L with special reference to their immunoglobulin G Fc-receptor activity.

    abstract::Of 33 streptococcal cultures belonging to serological group L, all bound human immunoglobulin (Ig) G, fibrinogen, and fibronectin; 32 bound bovine IgG; 31 bound alpha 2-macroglobulin; 5 bound albumin; and none bound either haptoglobin or IgA. The binding sites for IgG could be isolated from the L streptococci by tryps...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-001

    authors: Lämmler C,Schaufuss P,Frede C,Blobel H

    更新日期:1988-01-01 00:00:00

  • Source tracking of Escherichia coli by 16S-23S intergenic spacer region denaturing gradient gel electrophoresis (DGGE) of the rrnB ribosomal operon.

    abstract::This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjec...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W07-083

    authors: D'Elia TV,Cooper CR,Johnston CG

    更新日期:2007-10-01 00:00:00

  • Cryptococcus festucosus sp. nov. a new hymenomycetous yeast in the Holtermannia clade.

    abstract::Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-099

    authors: Golubev WI,Sampaio JP,Alves L,Golubev NW

    更新日期:2004-12-01 00:00:00

  • Dynamic metabolomic responses of Escherichia coli to nicotine stress.

    abstract::Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolo...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2014-0206

    authors: Ding L,Chen J,Zou J,Zhang L,Ye Y

    更新日期:2014-08-01 00:00:00

  • Deletion of the Candida albicans G-protein-coupled receptor, encoded by orf19.1944 and its allele orf19.9499, produces mutants defective in filamentous growth.

    abstract::Filamentous growth of Candida albicans occurs in response to a variety of environmental signals. The C. albicans gene orf19.1944 and its allele orf19.9499 are identical and are predicted to encode an 823-residue, 7-transmembrane-domain protein that has all the expected features of a G-protein-coupled receptor. The pro...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-095

    authors: Sciascia QL,Sullivan PA,Farley PC

    更新日期:2004-12-01 00:00:00

  • Do methanotrophs drive phosphorus mineralization in soil ecosystem?

    abstract::Experiments were carried out to elucidate linkage between methane consumption and mineralization of P from different phosphorous sources. The treatments were no CH4 no P amendment absolute control, with CH4 no P amendment control, with CH4 + inorganic P as Ca3(PO4)2 and with CH4 + organic P (sodium phytate). P sources...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2020-0254

    authors: Mohanty SR,Kumar A,Parmar R,Dubey G,Patra AK,Kollah B

    更新日期:2020-12-10 00:00:00

  • Cell-free, protein-synthesizing system of photosynthetic and heterotrophic Rhodospirillum rubrum.

    abstract::An active in vitro protein-synthesizing system has been developed from Rhodospirillum rubrum grown under either photosynthetic or heterotrophic conditions. A protease activity has been found in both of these systems, and this activity can be readily inactivated by treating the cells with KCl and phenylmethyl sulfonylf...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-042

    authors: Chow CT

    更新日期:1976-02-01 00:00:00