Abstract：
:Mice immunized subcutaneously with 10(6) viable yeast cells of Histoplasma capsulatum exhibited host protection to a challenge infection and also exhibited concomitant manifestations of cellular immunity to a culture filtrate antigen (yeast extract dialysate (YED)-histoplasmin). Protective immunity was determined by quantitative culture of organisms from infected spleens on a solid medium containing a growth factor produced by the organism. As early as 4 days following immunization, host protection was observed in mice. Positive footpad swelling, indicative of delayed-type hypersensitivity, also was apparent at the early time period (4-7 days) after immunization. By days 14 and 21 following immunization, the mice exhibited maximum footpad responses and maximum splenic clearance of yeast cells. As an additional correlate of cellular immunity, YED-histoplasmin was coupled with chromic chloride to murine erythrocytes (M-RBC) and mixed with immune or nonimmune splenic lymphocytes to detect antigen-specific rosette forming cells (RFC's). Only splenic lymphocytes from mice immunized with H. capsulatum formed antigen-specific RFC's (greater than or equal to 0.2%). Pretreatment of splenic lymphocytes with antitheta (theta) serum plus complement greatly reduced the numbers of RFC's, illustrating their T cell nature. Host protection and cell-mediated immune responses decreased substantially by day 28 following immunization.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Burt WR,Smith RAdoi
10.1139/m83-016subject
Has Abstractpub_date
1983-01-01 00:00:00pages
102-7issue
1eissn
0008-4166issn
1480-3275journal_volume
29pub_type
杂志文章abstract：:Southern bean mosaic virus (SBMV) protein was cleaved with cyanogen bromide and a highly basic peptide, CB-1, was isolated by ion exclusion and ion-exchange chromatography. Twelve peptides were separated from a tryptic digest of CB-1 by ion-exchange chromatography and the composition of these peptides was similar to t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-241
更新日期：1980-12-01 00:00:00
abstract：:Shiga toxins produced by Escherichia coli O157:H7 cause a wide spectrum of enteric diseases, such as lethal hemorrhagic colitis and hemolytic uremic syndrome. In this study, the B subunit protein of Shiga toxin type 1 (Stx1) was produced in the E. coli system, was further purified by Ni-column Affinity Chromatography ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W10-087
更新日期：2010-12-01 00:00:00
abstract：:When Caryophanon latum was exposed to egg white lysozyme in isotonic sucrose and observed by phase-contrast microscopy, protoplasts emerged along the length of the trichomes, apparently at sites corresponding to cross septa. Electron microscopy of sections revealed that this enzyme initially attacked the core of the s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-024
更新日期：1975-02-01 00:00:00
abstract：:Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-173
更新日期：1987-11-01 00:00:00
abstract：:Pseudomonas aeruginosa establishes life-long chronic infections in the cystic fibrosis (CF) lung by utilizing various adaptation strategies. Some of these strategies include altering metabolic pathways to utilize readily available nutrients present in the host environment. The airway sputum contains various host-deriv...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0485
更新日期：2014-12-01 00:00:00
abstract：:A visual micromethod for measuring antifungal effects on germination and growth is described. The antifungal agent griseofulvin and the fungus Trichophyton mentagrophytes were used as materials to compare the micromethod with a standard assay based on dry mycelial weight. The micromethod was more sensitive than the we...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-093
更新日期：1978-05-01 00:00:00
abstract：:Five media and procedures were compared for their recovery of fecal streptococci on membrane filters. Growth and numbers of a variety of pure cultures were compared, and recoveries from raw sewage and sewage effluent were determined on the five media. A total of 512 isolates were identified and the specificity of each...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-219
更新日期：1980-11-01 00:00:00
abstract：:The nucleotide sequence of the murE gene encoding the diaminopimelic acid adding enzyme of Escherichia coli is reported. The coding region consisted of 1413 base pairs and was separated from the ftsI (penicillin-binding protein 3) gene by 61 base pairs. The deduced primary structure of MurE comprised 471 amino acid re...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-175
更新日期：1989-11-01 00:00:00
abstract：:Aluminum (Al) toxicity is a major problem affecting soil fertility, microbial diversity, and nutrient uptake of plants. Rhizobia response and legume interaction under Al conditions are still unknown; it is important to understand how to develop and improve legume cultivation under Al stress. In this study, rhizobia re...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0019
更新日期：2018-08-01 00:00:00
abstract：:Candida albicans biofilm formation is considered an important matter because it can lead to strong resistance to conventional antifungal agents. Hyphae formed by C. albicans can also act as an important virulence factor related to its biofilm. The objective of this study was to determine the effect of zerumbone, a mon...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0155
更新日期：2019-10-01 00:00:00
abstract：:To isolate thiamine excretors and (or) overproducers, 188 cultures belonging to nine yeast and three fungal genera were screened. Nine excreted thiamine as determined by both the presence of cross-feeding zones on a thiamine-free agar medium seeded with a thiamine-requiring yeast strain and by the direct detection of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-189
更新日期：1992-11-01 00:00:00
abstract：:In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-186
更新日期：1988-09-01 00:00:00
abstract：:The environmental distribution of Giardia spp. and Cryptosporidium spp. is dependent upon human, agricultural, and wildlife sources. The significance of each source with regard to the presence of parasites in the environment is unknown. This 2-year study examined parasite prevalence in human sewage influent, wildlife,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-047
更新日期：2002-06-01 00:00:00
abstract：:Fifty-two killer, factor-resistant, nuclear mutants were isolated from sensitive strains of yeast and assorted into three functional groups. All but one mutant owed their resistance to an alteration in the cell wall binding site for killer. In several mutant strains, an alteration at the site of killer binding was ass...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-041
更新日期：1978-03-01 00:00:00
abstract：:Neisseria gonorrhoeae strains can be grouped or differentiated (auxotyped) by their requirements for none, or any one or more of proline, uracil, hypoxanthine, and citrulline or ornithine. Most strains were readily assessed because they responded with growth or no growth on each defined auxanographic medium. Other str...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-204
更新日期：1983-10-01 00:00:00
abstract：:The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-033
更新日期：1992-03-01 00:00:00
abstract：:Symbiotic nitrogen fixation may be limited by the transport of C4 dicarboxylates into bacteroids in the nodule for use as a carbon and energy source. In an attempt to increase dicarboxylate transport, a plasmid was constructed in which the Rhizobium meliloti structural transport gene dctA was fused to a tryptophan ope...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-091
更新日期：1992-06-01 00:00:00
abstract：:Morphological differentiation has commanded attention for its putative impact on the pathogenesis of invasive fungal infections. We evaluated in vitro and in vivo the dimorphism from mycelial to yeast-phase of Sporothrix schenckii, Blastomyces dermatitidis and Paracoccidioides brasiliensis isolates, two strains for ea...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-026
更新日期：2004-06-01 00:00:00
abstract：:Strict anaerobic cultures of Mucor hiemalis lead to major modifications of the quantitative and qualitative composition of sterols. Sterols of which the lateral chain contains an ethyl or ethylidene group in C24 are for the first time brought to evidence in a Mucorales. Isolation in the same conditions of E-ethylidene...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-095
更新日期：1983-05-01 00:00:00
abstract：:The aim of the present investigation was to isolate a probiotic strain from 23 samples of yurts cheese and 21 samples of kumiss (collected from scattered households in Xinjiang and Inner Mongolia), and from eN-Lac Capsules, a health-promoting product. The isolates were subjected to biochemical characterization analysi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0383
更新日期：2014-11-01 00:00:00
abstract：:The purpose of this study was to examine expression and regulation of 6 multidrug efflux systems, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY, MexJK, and MexVW, in 13 non-cystic fibrosis (CF) clinical isolates of Pseudomonas aeruginosa. These isolates displayed a high level of resistance to many clinically imp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0239
更新日期：2014-07-01 00:00:00
abstract：:A dairy product processing plant was studied for 2.5 years to examine contamination with Staphylococcus aureus and try to correlate the source of contamination. Cultures were submitted to an antibiotic susceptibility test (AST) and characterised by Pulsed-field Gel Electrophoresis (PFGE) analysis. Results showed that ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-111
更新日期：2000-12-01 00:00:00
abstract：:The production of cold shock proteins (csps) and cold acclimation proteins (caps) was characterized in the psychrotrophic bacterium Pseudomonas putida Q5 and its transconjugant P. putida Q5T which contains the toluene-degradative TOL (pWWO) plasmid, using two-dimensional gel electrophoresis and computing scanning lase...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-100
更新日期：1996-08-01 00:00:00
abstract：:The effects of various hydrocarbon substrates, and a chemical surfactant capable of enhancing crude-oil biodegradation, on the community structure of a mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% were identified at the genus level or better by phospholipid fatty acid...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:Fungal cultures were grown by a new microslide method and by the traditional slide-culture method. Sporulation results were similar by both methods. The microslide method has the advantages of increased safety to the user and ease of storage. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-204
更新日期：1978-10-01 00:00:00
abstract：:The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation pat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-090
更新日期：1990-07-01 00:00:00
abstract：:Catalase from Bacillus sp. N2a (BNC) isolated from Antarctic seawater was purified to homogeneity. BNC has a molecular mass of about 230 kDa and is composed of four identical subunits of 56 kDa. The catalase showed optimal activity at 25 degrees C and at a pH range of 6-11. The enzyme could be inhibited by azide, hydr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-066
更新日期：2008-10-01 00:00:00
abstract：:A total of 230 strains of metal-tolerant bacteria from water and sediment samples collected in Chesapeake Bay were isolated on a medium containing cobalt, lead, mercury, or molybdenum. In addition, a set of 71 cultures were simultaneously isolated on glucose tryptone yeast extract agar medium without metals. Twenty-th...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-212
更新日期：1977-10-01 00:00:00
abstract：:Ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. All test isolates produced siderophores as indicated by a positive reaction in the FeCl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. Further, the test isolates were compar...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-012
更新日期：2006-06-01 00:00:00
abstract：:Yersinia pestis is a bacterium that is transmitted between fleas, which have a body temperature of 26 °C, and mammalian hosts, which have a body temperature of 37 °C. To adapt to the temperature shift, phenotype variations, including virulence, occur. In this study, an antigen microarray including 218 proteins of Y. p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-007
更新日期：2011-04-01 00:00:00
