Abstract：
:To determine the subcellular location of the Streptococcus mutans P1 protein C-terminal anchor, cell envelope fractionation experiments were conducted in combination with Western immunoblotting, using monoclonal antibody MAb 6-8C specific for an epitope that maps near the C terminus of P1 protein and also a polyclonal antibody preparation directed against the P1 C-terminal 144 amino acids (P1COOH). P1 protein was detected in cell walls but not the membrane purified from S. mutans cells by the monoclonal antibody. In contrast, P1 protein was not detected in the same cell wall preparation using the anti-P1COOH polyclonal antibody. However, proteins released from the cell walls by treatment with mutanolysin contained antigen that was recognized by the anti-P1COOH antibody, suggesting that the epitopes recognized by the antibody were masked by peptidoglycan in the cell wall preparations. When cell walls were treated with boiling trichloroacetic acid to solubilize cell-wall-associated carbohydrate, P1 antigen could not be detected in either the solubilized carbohydrate, or in the remaining peptidoglycan, regardless of whether polyclonal or monoclonal antibody was used. However, when the peptidoglycan was treated with mutanolysin, P1 antigen could be detected in the mutanolysin solubilized fraction by MAb 6-8C. Collectively, these data suggest that the C-terminal 144 amino acids of the P1 protein are embedded within the cell wall, and associated exclusively with the peptidoglycan. Furthermore, the ability of the anti-P1COOH antibody to recognize P1 antigen only after mutanolysin treatment of cell walls suggests these C-terminal 144 amino acids are tightly intercalated within the peptidoglycan strands.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Homonylo-McGavin MK,Lee SF,Bowden GHsubject
Has Abstractpub_date
1999-06-01 00:00:00pages
536-9issue
6eissn
0008-4166issn
1480-3275journal_volume
45pub_type
杂志文章abstract：:We examined isolates from 4 commercial bioinsecticides based on different strains of Bacillus thuringiensis subspecies (kurstaki, israelensis, aizawai, and tenebrionis) for the presence of genes encoding proteins with known enterotoxigenicity (nhe, hbl, cytk, ces) and various other putative virulence genes (piplc, sph...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-106
更新日期：2007-12-01 00:00:00
abstract：:The purpose of this study was to determine the prevalence of antibiotic-resistant bacteria and endotoxin in soil after land application of biosolids. Soil was collected over a 15 month period following land application of biosolids, and antibiotic resistance was ascertained using clinically relevant antibiotic concent...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-021
更新日期：2007-05-01 00:00:00
abstract：:Natural enrichments of magnetotactic bacteria were used to study the sites where heavy metals accumulate in uncultured bacteria. Most bacteria obtained by magnetic concentration from these enrichments contained, in addition to the magnetosomes, large phosphorus-rich granules in the cytoplasm. Metal (Zn, Mn, Sr, Cd, Al...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：:Dermatophytes are a group of fungi that are capable of invading keratinized tissues of humans and other animals. Antifungal susceptibility analysis and genetic studies by random amplification of polymorphic DNA (RAPD), have been used to detect polymorphism as well as determining the possible resistance of dermatophyte...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-024
更新日期：2005-06-01 00:00:00
abstract：:In our study of opportunistic pathogens, we have some indication that Staphylococcus aureus can increase resistance in mice against Pseudomonas aeruginosa. Intraperitoneal injections of sublethal doses of S. aureus had a protective effect in mice against lethal doses of P. aeruginosa, more so if living and coagulase-p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1976-07-01 00:00:00
abstract：:Transcriptional profiles of 2 unrelated clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were analyzed following 10% (v/v) ethanol challenge (15 min), which arrested growth but did not reduce viability. Ethanol-induced stress (EIS) resulted in differential gene expression of 1091 genes, 600 common ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2017-0221
更新日期：2017-09-01 00:00:00
abstract：:HI plasmids are distinguished by their thermosensitive mode of conjugation (transfer efficiency is optimal at 22-30 degrees C) and their capacity to encode multiple antibiotic resistance. These traits have implicated HI plasmids as potential vectors in the dissemination of antibiotic resistance among pathogenic and in...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-084
更新日期：1993-06-01 00:00:00
abstract：:Lysis of mid-log phase cells of the Gram-positive bacterium, Corynebacterium sepedonicum, by a combination of lysozyme treatment and incubation with alkaline sodium dodecyl sulfate at 56 degrees C led to the recovery of a single plasmid. The plasmid was purified in CsCl density gradients, and its molecular weight esti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-116
更新日期：1986-08-01 00:00:00
abstract：:Proteins were solubilized from cell wall fractions of Candida albicans and separated by polyacrylamide gel electrophoresis. Cell walls were isolated from 25 and 37 degrees C growing and stationary phase yeast cultures and from germ tubes. The 42 protein bands detected by dye binding were observed in all wall extracts,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-220
更新日期：1983-10-01 00:00:00
abstract：:Samples of seawater, sediment, and the following invertebrates were collected from the Mediterranean Sea in the neighborhood of Alexandria, Egypt: sea urchins (Echinus spp.), clams (Tapes spp.), and wedge shells (Donax trunculus). A total of 165 samples were collected from November 1979 to July 1980. Average counts (p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-187
更新日期：1982-11-01 00:00:00
abstract：:The rapid onset of resistance reduces the efficacy of most conventional antimicrobial drugs and is a general cause of concern for human well-being. Thus, there is great demand for a continuous supply of novel antibiotics to combat this problem. Bacteria-derived antimicrobial peptides (AMPs) have long been used as food...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/cjm-2014-0613
更新日期：2015-02-01 00:00:00
abstract：:In the divE mutant, which has a temperature-sensitive mutation in the tRNA1(Ser) gene, the synthesis of beta-galactosidase is dramatically decreased at the non-permissive temperature. In Escherichia coli, the UCA codon is only recognized by tRNA1(Ser). Several genes containing UCA codons are normally expressed at 42 d...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:Responses of Rhizoctonia solani to herbicides in soil cultures were assessed by measuring soil enzyme activity and other growth-related factors. Both beta-galactosidase (EC 3.2.1.23) and phosphatase (EC 3.1.3.1.3.1.3.2) activities were highly correlated with amounts of mycelium in soil. Both enzyme activities were red...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-089
更新日期：1977-05-01 00:00:00
abstract：:Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w98-225
更新日期：1999-02-01 00:00:00
abstract：:This study was undertaken to determine if direct cytotoxicity (DC) against herpes simplex virus infected cells, perhaps mediated by T cells, could be demonstrated in individuals subject to recurrent herpes labialis. The mononuclear cells from 7 out of 17 individuals with recurrent herpes expressed DC whereas no DC was...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-177
更新日期：1978-09-01 00:00:00
abstract：:In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-188
更新日期：1979-10-01 00:00:00
abstract：:To assess the effect of fire and salvage logging on the diversity of mycorrhizal-bacterial communities, bacteria associated with Cenococcum, Thelephora, Tomentella, Russulaceae, and E-strain ectomycorrhizae (ECM) of Abies lasiocarpa seedlings were characterized using two approaches. First, bacteria were isolated and c...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-056
更新日期：2002-07-01 00:00:00
abstract：:Bacterial diversity was assessed in water samples collected from several uranium mining wastes in Ger many and in the United States by using 16S rDNA and ribosomal intergenic spacer amplification retrievals. The results obtained using the 16S rDNA retrieval showed that the samples collected from the uranium mill taili...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-082
更新日期：2005-11-01 00:00:00
abstract：:Leptospira interrogans serotype pomona was isolated from the kidneys of a normal striped skunk (Mephitis mephitis hudsonicus) collected near Kindersley, Saskatchewan. Although there were no gross lesions, small foci of chronic interstitial nephritis were observed microscopically. This is the first reported isolation o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-237
更新日期：1977-12-01 00:00:00
abstract：:Antibiotic-resistant and antimetabolite-resistant mutants of the nitrogen-fixing symbiotic bacterium Frankia were isolated to provide strains with genetic backgrounds amenable to genetic analysis. The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV light on four Frankia strains were investigated. U...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-013
更新日期：2004-04-01 00:00:00
abstract：:The effects of various hydrocarbon substrates, and a chemical surfactant capable of enhancing crude-oil biodegradation, on the community structure of a mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% were identified at the genus level or better by phospholipid fatty acid...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:The goal of this study was to evaluate the antibiofilm and antimicrobial activities of Bacoside A, a formulation of phytochemicals from Bacopa monnieri, against Staphylococcus aureus and Pseudomonas aeruginosa, which are known to form biofilms as one of their virulence traits. The antimicrobial effects of Bacoside A w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0365
更新日期：2017-02-01 00:00:00
abstract：:The catalytic subunit of many Escherichia coli redox enzymes bares a twin-arginine translocation (Tat)-dependent signal peptide in its precursor, which directs the redox enzyme complex to this Sec-independent pathway. NarG of the E. coli nitrate reductase NarGHI complex possesses a vestige twin-arginine motif at its N...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-111
更新日期：2009-02-01 00:00:00
abstract：:The anaerobic degradative pathway for metabolism of 2,4,6-trinitrotoluene (TNT) by a consortium of Desulfovibrio spp. isolated from a creek sediment was studied. This consortium has the metabolic capability to degrade TNT to fatty acids. The growth of the consortium and the metabolism of TNT were greatly enhanced in t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-155
更新日期：1996-12-01 00:00:00
abstract：:Highly distinctive aspects of the exponentail-phase Rhizobium japonicum cell were disclosed by means of thin sections, freeze etching, fluorescent antibodies, and ruthenium red staining. Polarity was expressed in the form of reserve polymer distribution near one end of the cell and as cytoplasmic localization near the...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-191
更新日期：1977-09-01 00:00:00
abstract：:A commercially available single-bottle blood culture system was evaluated at Ben Taub General Hospital, a Harris County District Hospital. Blood cultures from 1010 patients were examined with the Lederle Diagnostics one-bottle blood culture medium-SPS, Columbia broth (E-Vac, Pfizer), and an in-house-prepared brain hea...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-155
更新日期：1977-08-01 00:00:00
abstract：:Actinomycetes DNAs were digested with restriction enzymes to study the presence of methylated bases. Analysis showed that the enterobacterial Dam and Dcm systems are absent. Methylation at the internal cytosine in CCGG sequences, typical of eukaryotes, was also absent. We also tested 18 restriction endonucleases recog...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-031
更新日期：1996-02-01 00:00:00
abstract：:In mycetomes of leafhoppers, Helochara communis, ultrastructural and histochemical studies revealed that spheroplast symbiotes (t-symbiotes) were converted to 'a-symbiotes' (so-called), with apparent loss of DNA--a phenomenon reminiscent of rhizobium-bacteroid conversion in certain legume nodules. Additional t-symbiot...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-028
更新日期：1975-02-01 00:00:00
abstract：:In this work, we disrupted one of three putative phosphatidylinositol phospholipase C genes of Aspergillus nidulans and studied its effect on carbon source sensing linked to vegetative mitotic nuclear division. We showed that glucose does not affect nuclear division rates during early vegetative conidial germination (...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-034
更新日期：2011-07-01 00:00:00
abstract：:The in situ degradation of the two nitramine explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), was evaluated using a mixture of RDX and HMX, incubated anaerobically at 10 degrees C with marine sediment from a previous military dumping site of unexplod...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-112
更新日期：2004-02-01 00:00:00
