Restriction analysis of actinomycetes chromosomal DNA.

abstract：:Phialocephala fortinii is a dark septate fungal endophyte that colonizes roots of many host species. Its effect on plant growth varies from being pathogenic to beneficial. The basic biology of this species has received little research, and thus the main objectives of this study were to determine cytological features o...

journal_title：Canadian journal of microbiology

authors： Saito K,Kuga-Uetake Y,Saito M,Peterson RL

更新日期：2006-07-01 00:00:00

abstract：:The Bacillus subtilis glutamyl-tRNA synthetase (GluRS), encoded by the gltX gene, aminoacylates its homologous tRNA(Glu) and tRNA(Gln) with glutamate. This gene was cloned with its sigma A promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and B. s...

journal_title：Canadian journal of microbiology

authors： Pelchat M,Lacoste L,Yang F,Lapointe J

更新日期：1998-04-01 00:00:00

abstract：:We examined isolates from 4 commercial bioinsecticides based on different strains of Bacillus thuringiensis subspecies (kurstaki, israelensis, aizawai, and tenebrionis) for the presence of genes encoding proteins with known enterotoxigenicity (nhe, hbl, cytk, ces) and various other putative virulence genes (piplc, sph...

journal_title：Canadian journal of microbiology

authors： Kyei-Poku G,Gauthier D,Pang A,van Frankenhuyzen K

更新日期：2007-12-01 00:00:00

abstract：:We partially purified and characterized the cytochrome aa3 from the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense. This cytochrome aa3 showed oxygen consumption activity with N, N, N', N'-tetramethyl-1,4-phenylenediamine and ascorbate as substrates, and also displayed bovine cytochrome c o...

journal_title：Canadian journal of microbiology

authors： Nunoura T,Sako Y,Wakagi T,Uchida A

更新日期：2005-08-01 00:00:00

abstract：:The physiochemical characteristics of the intracellular proteolytic enzymes of Oidiodendron kalari, a neuropathogenic fungus, were studied. The organism in the yeast phase was grown in a semisynthetic medium containing 1% tryptone, at 37 degrees C for 48 hr, on a gyrotory shaker. The crude extract was prepared by brea...

journal_title：Canadian journal of microbiology

authors： Cino PM,Tewari RP

更新日期：1975-09-01 00:00:00

abstract：:Aphanomyces astaci secondary cyst walls and walls of germinating spores were prepared by alkaline hydrolysis, autolysis, sonication, and enzymic degradation and were examined by shadow-casting and negative-staining techniques. The cyst wall consists of randomly oriented fibrils, about 3 nm in diameter. The fibrils are...

journal_title：Canadian journal of microbiology

authors： Nyhlén L,Unestam T

更新日期：1978-11-01 00:00:00

abstract：:Two molecular forms of alpha-galactosidase (EC 3.2.1.22) synthesized constitutively by Bacillus stearothermophilus, strain AT-7, have been purified. alpha-Galactosidase I (with the substrate p-nitrophenyl alpha-D-galactopyranoside (PNPG)) has a pH optimum of 6 and half-life at 65 degrees C of > 2 h at low protein conc...

journal_title：Canadian journal of microbiology

authors： Pederson DM,Goodman RE

更新日期：1980-08-01 00:00:00

abstract：:To expand current knowledge on the molecular aspects of alkaline phosphatase PhoX in shallow eutrophic freshwaters, we investigated the genetic diversity and abundance of the PhoX-encoding gene (phoX) in 4 ecological regions in Lake Taihu, China, following a gradient in total phosphorus concentrations ranging from hyp...

journal_title：Canadian journal of microbiology

authors： Dai J,Chen D,Gao G,Tang X,Wu S,Wu X,Zhou J

更新日期：2014-03-01 00:00:00

abstract：:Viruses were isolated from every sample of raw (100 L) and treated (1000 L) water collected at a water treatment plant drawing sewage-contaminated river water. Few plaque-forming isolates were formed but cytopathogenic viruses were isolated as frequently in drinking water as in raw water. In drinking water some sample...

journal_title：Canadian journal of microbiology

authors： Payment P

更新日期：1981-04-01 00:00:00

abstract：:Cultured alveolar and peritoneal macrophages obtained from normal and immunized rabbits were used to induce wall-defective microbial variants of Pseudomonas pseudomallei. Variants were iduced only by normal alveolar macrophages. The variants reverted to typical Pseudomonas forms either spontaneously or upon transfer i...

journal_title：Canadian journal of microbiology

authors： Kishimoto RA,Eveland WC

更新日期：1975-12-01 00:00:00

abstract：:The purpose of this study was to determine the prevalence of antibiotic-resistant bacteria and endotoxin in soil after land application of biosolids. Soil was collected over a 15 month period following land application of biosolids, and antibiotic resistance was ascertained using clinically relevant antibiotic concent...

journal_title：Canadian journal of microbiology

authors： Brooks JP,Maxwell SL,Rensing C,Gerba CP,Pepper IL

更新日期：2007-05-01 00:00:00

abstract：:Fetal bovine serum (FBS) is a necessary constituent of the culture media employed to foster the growth of Trypanosoma cruzi epimastigote forms. In different laboratories, the serum is used at final concentrations of 5 or 10%. We have normally supplemented the complex medium with 10% FBS. Under this condition we have d...

journal_title：Canadian journal of microbiology

authors： Racagni G,de Lema MG,Hernández G,Machado-Domenech EE

更新日期：1995-10-01 00:00:00

abstract：:For the first time, an Escherichia coli strain producing four microcins (Mcc), B17, D93, J25, and L, and showing immunity to Mcc V was isolated and characterized. Each of the gene clusters encoding the production of Mcc B17, D93, and L was cloned separately. The gene cluster for Mcc L was cloned within a 13.5-kb HindI...

journal_title：Canadian journal of microbiology

authors： Sablé S,Duarte M,Bravo D,Lanneluc I,Pons AM,Cottenceau G,Moreno F

更新日期：2003-05-01 00:00:00

abstract：:During growth on minimal salts - glucose media supplemented with high concentrations of Na2SO3 (10-3 and 10-2 M), Salmonella heidelberg exhibited cytological and growth responses which indicated increased cellular toxicity with increasing sulfite concentrations. The large quantities of sulfide evolved during growth at...

journal_title：Canadian journal of microbiology

authors： McCready RG,Krouse HR

更新日期：1979-12-01 00:00:00

abstract：:Growth of Neisseria perflava, Neisseria cinerea, and Neisseria sicca strain Kirkland in media supplemented with sucrose (0.5 to 5.0% w/v) resulted in the formation of giant cells. Response to sucrose was specific in that a variety of other carbohydrates did not mediate giant cell formation. Giant cells appeared only u...

journal_title：Canadian journal of microbiology

authors： Johnson KG,McDonald IJ

更新日期：1976-03-01 00:00:00

abstract：:A moderately halophilic Acinetobacter sp., capable of producing dextrinogenic amylase, was isolated from sea-sands. Maximum enzyme production was obtained when the bacterium was cultivated aerobically in media containing 1 to 2M NaCl or 1M KCl. Two kinds of amylase, amylases I and II were purified from the culture fil...

journal_title：Canadian journal of microbiology

authors： Onishi H,Hidaka O

更新日期：1978-09-01 00:00:00

abstract：:A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate deter...

journal_title：Canadian journal of microbiology

authors： Rainio A,Vahvaselkä M,Suomalainen T,Laakso S

更新日期：2001-08-01 00:00:00

abstract：:A mesophilic anaerobe, a member of the Bacteroidaceae family (NRC2248), isolated from a cellulose-enrichment culture, digested untreated Whatman cellulose powder and HCl-treated cotton battings while producing hydrogen, carbon dioxide, cellobiose, glucose, and acetic acid as the sole volatile acid. This organism also ...

journal_title：Canadian journal of microbiology

authors： Saddler JN,Khan AW

更新日期：1979-12-01 00:00:00

abstract：:A microplaque reduction assay for human and mouse interferons is described, using plastic disposable multicompartmental plates with flat-bottomed wells of 6 mm in diameter. The procedure is rapid and reproducible and lends itself to a considerable degree of automation. Its potential application in other areas of virol...

journal_title：Canadian journal of microbiology

authors： Campbell JB,Grunberger T,Kochman MA,White SL

更新日期：1975-08-01 00:00:00

abstract：:Fungal chitinases play essential roles in chitin degradation, cell wall remodeling, chitin recycling, nutrition acquisition, autolysis, and virulence. In this study, 18 genes of the glycoside hydrolase 18 (GH18) family were identified in the Isaria cicadae genome. Seventeen of the genes belonged to chitinases and one ...

journal_title：Canadian journal of microbiology

authors： Peng Y,Wang L,Gao Y,Ye L,Xu H,Li S,Jiang J,Li G,Dang X

更新日期：2020-04-01 00:00:00

abstract：:Roots of field-grown tropical maize, Panicum maximum Jacq. and Digitaria decumbens Stent., and of sorghum and wheat grown in monoxenic culture with the diazotroph Spirillum lipoferum (syn. Azospirillum spp.) were examined for tetrazolium-reducing bacteria following incubation of roots in a malate-phosphate buffer-2,3,...

journal_title：Canadian journal of microbiology

authors： Patriquin DG,Döbereiner J

更新日期：1978-06-01 00:00:00

abstract：:In Escherichia coli, the N-terminal domain of the essential protein FtsK (FtsKN) is proposed to modulate septum formation through the formation of dynamic and essential protein interactions with both the Z-ring and late-stage division machinery. Using genomic mutagenesis, complementation analysis, and in vitro pull-do...

journal_title：Canadian journal of microbiology

authors： Berezuk AM,Roach EJ,Seidel L,Lo RY,Khursigara CM

更新日期：2020-04-01 00:00:00

abstract：:Bacterial strains and consortia of bacteria have been isolated for their ability to degrade, under anaerobic conditions, homocyclic monoaromatic compounds, such as phenolic compounds, methylbenzenes, and aminobenzenes. As opposed to aerobic conditions where these compounds are degraded via dihydroxyl intermediates int...

journal_title：Canadian journal of microbiology

authors： Villemur R

更新日期：1995-10-01 00:00:00

abstract：:Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obta...

journal_title：Canadian journal of microbiology

authors： Armon R,Arella M,Payment P

更新日期：1988-05-01 00:00:00

abstract：:Klebsiella pneumoniae was found to effect the reductive dechlorination of methoxychlor (2,2-bis(p-methoxyphenyl)-1, 1, 1-trichloroethane) and its dihydroxy derivative (2, 2-bis (p-hydroxyphenyl)-1, 1, 1-trichloroethane) to dichloro derivatives 2, 2-bis (p-methoxyphenyl)-1, 1-dichloroethane and 2, 2-bis (p-hydroxypheny...

journal_title：Canadian journal of microbiology

authors： Baarschers WH,Bharath AI,Elvish J,Davies M

更新日期：1982-02-01 00:00:00

abstract：:The antibacterial mechanism of a Cinnamomum cassia essential oil from Vietnam and of its main component (trans-cinnamaldehyde, 90% (m/m) of C. cassia essential oil) against a Listeria innocua strain was investigated to estimate their potential for food preservation. In the presence of C. cassia essential oil or trans-...

journal_title：Canadian journal of microbiology

authors： Trinh NT,Dumas E,Thanh ML,Degraeve P,Ben Amara C,Gharsallaoui A,Oulahal N

更新日期：2015-04-01 00:00:00

abstract：:An obligate methyltroph Methylomonas methylovora oxidized methylamine, formaldehyde, and formate. Enzymes oxidizing these substrates were detected in a cell-free system. Phenazine methosulfate-linked methylamine dehydrogenase was purified 21-fold. The enzyme had optimum activity at pH 7.5 and was stable at 60 degrees ...

journal_title：Canadian journal of microbiology

authors： Mehta RJ

更新日期：1977-04-01 00:00:00

abstract：:Giardia duodenalis is a common intestinal parasite in most parts of the world. In Canada it is associated with both endemic and epidemic infections that are often transmitted by the waterborne route. Although G. duodenalis strains have been isolated from several animals, the role of other mammals in human infection is...

journal_title：Canadian journal of microbiology

authors： Wenman WM,Meuser RU,Wallis PM

更新日期：1986-12-01 00:00:00

abstract：:The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on beta-glucuronidase activity, using the fluorogenic substrat...

journal_title：Canadian journal of microbiology

authors： Shadix LC,Rice EW

更新日期：1991-12-01 00:00:00

abstract：:Bacterial biofilms are commonly formed on medical devices and food processing surfaces. The antimicrobials used have limited efficacy against the biofilms; therefore, new strategies to prevent and remove these structures are needed. Here, the effectiveness of brief oxidative treatments, based on the combination of sod...

journal_title：Canadian journal of microbiology

authors： Olmedo GM,Grillo-Puertas M,Cerioni L,Rapisarda VA,Volentini SI

更新日期：2015-05-01 00:00:00