Abstract:
:The purpose of this study was to determine the prevalence of antibiotic-resistant bacteria and endotoxin in soil after land application of biosolids. Soil was collected over a 15 month period following land application of biosolids, and antibiotic resistance was ascertained using clinically relevant antibiotic concentrations. Ampicillin, cephalothin, ciprofloxacin, and tetracycline resistance were all monitored separately for any changes throughout the 15 month period. Endotoxin soil concentrations were monitored using commercially available endotoxin analysis reagents. Overall, land application of biosolids did not increase the percentage of antibiotic-resistant culturable bacteria above background soil levels. Likewise, land application of biosolids did not significantly increase the concentration of endotoxin in soil. This study determined and established a baseline understanding of the overall effect that land application of biosolids had on the land-applied field with respect to antibiotic-resistant bacterial and endotoxin soil densities.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Brooks JP,Maxwell SL,Rensing C,Gerba CP,Pepper ILdoi
10.1139/W07-021subject
Has Abstractpub_date
2007-05-01 00:00:00pages
616-22issue
5eissn
0008-4166issn
1480-3275pii
w07-021journal_volume
53pub_type
杂志文章abstract::Staphylococcus aureus was grown in a rich peptone medium which became alkaline with continued incubation. Cells were grown at 37 degrees C and in the same medium containing 1 M NaCl at 46 degrees C, a temperature at which this organism can grow only when protected by NaCl. Cells of these cultures are hereafter called ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-049
更新日期:1983-03-01 00:00:00
abstract::The B subunit of Escherichia coli heat-labile enterotoxin (LTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral adjuvant that induces a strong immune response against co-administered or coupled antigens. In this paper, the effect of LTB on the humoral immune response to rec...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-056
更新日期:2008-08-01 00:00:00
abstract::No clear data are available on how flavonoids from different chemical groups affect root colonization by arbuscular mycorrhizal fungi (AMF) and whether flavonoids affecting the presymbiotic growth of AMF also affect root colonization by AMF. In the present work, we compared the effect of flavones (chrysin and luteolin...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-036
更新日期:2007-06-01 00:00:00
abstract::Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-021
更新日期:1989-01-01 00:00:00
abstract::The in situ degradation of the two nitramine explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), was evaluated using a mixture of RDX and HMX, incubated anaerobically at 10 degrees C with marine sediment from a previous military dumping site of unexplod...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-112
更新日期:2004-02-01 00:00:00
abstract::Three distinct bacterial populations have been defined in the bovine rumen: the rumen fluid population; the population associated with food particles; and the population adherent to the rumen epithelium. Alkaline phosphatase activity has been reported in cells of the first two populations and here we report that assay...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-140
更新日期:1979-08-01 00:00:00
abstract::A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w99-055
更新日期:1999-08-01 00:00:00
abstract::A defined medium (CDM) is described which supported growth and sporulation of type E strains of Clostridium botulinum, but not sporulation of other serotypes of C. botulinum or C. sporogenes. As compared to growth in complex medium, spore outgrowth was delayed and both the growth rate and the cell yield was reduced. H...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-076
更新日期:1979-04-01 00:00:00
abstract::The aim of the present study was to examine soil samples from various vegetation zones in terms of physicochemical properties, microbial communities, and isolation and identification (by polymerase chain reaction and transmission electron microscopy) of bacteria producing poly-β-hydroxybutyrates (PHBs). Soil samples w...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-037
更新日期:2012-05-01 00:00:00
abstract::The aim of this study was to unravel, by focusing on cell surface properties, the underlying virulence factors contributing to the difference in the pathogenicity observed in two Acinetobacter baumannii strains isolated from the same patient. The two strains were phenotypically different: (i) a mucoid strain (AB-M),...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-131
更新日期:2012-03-01 00:00:00
abstract::To determine the seroprevalence of Norwalk virus and whether Norwalk virus contributed to an observed increase in illness in tap water drinkers participating in a prospective epidemiological study, sera collected during the study were examined for changes in Norwalk virus antibody titer, using a specific enzyme immuno...
journal_title:Canadian journal of microbiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1139/m94-128
更新日期:1994-10-01 00:00:00
abstract::Asexual sporulation in the fungus Aspergillus nidulans is controlled, in part, by a central regulatory pathway composed of the brlA, abaA, and wetA genes. The coding region of each of these genes was fused, in frame, to the threonine-inducible alcohol dehydrogenase promotor then stably incorporated into the A. nidulan...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-164
更新日期:1994-12-01 00:00:00
abstract::To determine the subcellular location of the Streptococcus mutans P1 protein C-terminal anchor, cell envelope fractionation experiments were conducted in combination with Western immunoblotting, using monoclonal antibody MAb 6-8C specific for an epitope that maps near the C terminus of P1 protein and also a polyclonal...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-027
更新日期:1993-02-01 00:00:00
abstract::The effects of water activity (aw, 0.994-0.90 identical to 0.4-14.0 (-)MPa water potential), temperature (4-45 degrees C), and pH (3.6, 5.5, 7.0), and their interactions on growth of isolates of Fusarium moniliforme and Fusarium proliferatum were determined in vitro on a maize extract agar medium. Growth of two isolat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-149
更新日期:1995-12-01 00:00:00
abstract::Large numbers of minute, flat, long, straight, but often broken structures were observed in aqueous extracts of soil. We have applied the purely descriptive term 'tubules' to these structure, because they apparently became flattened during preparation for electron-microscopic viewing. The tubules appeared to be compos...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-105
更新日期:1979-06-01 00:00:00
abstract::Seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (IA and IB), two on day 4 (IVA and IVB), one on day 8 (VIIIA), and two on day 9 (IXA and IXB). The lambs were reared in these isolators until ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-064
更新日期:1989-03-01 00:00:00
abstract::The activities of isocitrate lyase, esterase, and lipase by the psychrotrophic Acinetobacter sp. strain HH1-1 were monitored during incubation at 25 degreesC, 5 degreesC, and after a 25 degreesC to 5 degreesC down shift in growth temperature. During growth at 25 degreesC, isocitrate lyase activity was detected in cell...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::Neisseria gonorrhoeae exist in transparent (Tr) and opaque (Op) colony forms. Op forms are recovered from patients early in the menstrual cycle; Tr colonies predominate late in the cycle. The mechanism for this colonial variation was examined by determining the influence of gonodal hormones on growth inhibition of Op ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-044
更新日期:1982-03-01 00:00:00
abstract::In British Columbia, Canada, drinking water is tested for total coliforms and Escherichia coli, but there is currently no routine follow-up testing to investigate fecal contamination sources in samples that test positive for indicator bacteria. Reliable microbial source tracking (MST) tools to rapidly test water sampl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0618
更新日期:2013-03-01 00:00:00
abstract::Three types of bacteria are associated with leprosy: Mycobacterium leprae, leprosy-derived corynebacteria (LDC), and armadillo-derived mycobacteria (ADM). The immunological relationships between these three types of bacteria and Mycobacterium bovis BCG, used as a reference, were determined by cross-immunoelectrophores...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-083
更新日期:1994-06-01 00:00:00
abstract::Enterococci, formerly confounded with faecal streptococci, are recognized since the beginning of the century as being faecal in origin and are generally searched for in waste waters and food products; their detection may in fact indicate the presence of enteropathogenic organisms. Although nearly ubiquitous, their pre...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::Glutaraldehyde, the biological fixative of choice in the cytochemical localization of the phosphatases, was investigated for its effects on Pseudomonas aeruginosa alkaline phosphatase. Comparative studies on the inactivation of alkaline phosphatase by glutaraldehyde showed significant differences when the purified pro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-078
更新日期:1981-05-01 00:00:00
abstract::This study, the first of arbovirus activity in Prince Edward Island, has shown antibodies to the snowshoe hare strain of the California encephalitis group virus to be present in sera of wild and domestic animals; 15.35% of snowshoe hare, 20% of equine. and 2.5% of bovine sera were positive for hemagglutination inhibit...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-188
更新日期:1981-11-01 00:00:00
abstract::A total of 230 strains of metal-tolerant bacteria from water and sediment samples collected in Chesapeake Bay were isolated on a medium containing cobalt, lead, mercury, or molybdenum. In addition, a set of 71 cultures were simultaneously isolated on glucose tryptone yeast extract agar medium without metals. Twenty-th...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-212
更新日期:1977-10-01 00:00:00
abstract::Fifty-two killer, factor-resistant, nuclear mutants were isolated from sensitive strains of yeast and assorted into three functional groups. All but one mutant owed their resistance to an alteration in the cell wall binding site for killer. In several mutant strains, an alteration at the site of killer binding was ass...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-041
更新日期:1978-03-01 00:00:00
abstract::Dermatophytes are a group of fungi that are capable of invading keratinized tissues of humans and other animals. Antifungal susceptibility analysis and genetic studies by random amplification of polymorphic DNA (RAPD), have been used to detect polymorphism as well as determining the possible resistance of dermatophyte...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-024
更新日期:2005-06-01 00:00:00
abstract::The effects of various hydrocarbon substrates, and a chemical surfactant capable of enhancing crude-oil biodegradation, on the community structure of a mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% were identified at the genus level or better by phospholipid fatty acid...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-008
更新日期:1992-01-01 00:00:00
abstract::Ninety-five fecal samples from Atlantic cod (Gadus morhua L.), caught along the northern Norwegian coast, were examined bacteriologically for occurrence of C. perfringens. Isolates were examined by polymerase chain reaction (PCR) for genes encoding the four lethal toxins (alpha, beta, epsilon, and iota) for classifica...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-015
更新日期:2002-04-01 00:00:00