Evaluation of beta-glucuronidase assay for the detection of Escherichia coli from environmental waters.

Abstract:

:The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on beta-glucuronidase activity, using the fluorogenic substrate 4-methylumbelliferyl beta-D-glucuronide (MUG). This study was conducted to determine whether beta-glucuronidase negative E. coli were present in significant numbers in environmental waters. Two hundred and forty E. coli cultures were isolated from 12 water samples collected from different environmental sources. beta-glucuronidase activity was determined using lauryl tryptose broth with MUG, EC broth with MUG, and the Autoanalysis Colilert (AC) procedure. The isolates were also evaluated by the standard EC broth gas fermentation method for fecal coliforms. The results confirm that assaying for the enzyme beta-glucuronidase utilizing the MUG substrate is an accurate method for the detection of E. coli in environmental waters.

journal_name

Can J Microbiol

authors

Shadix LC,Rice EW

doi

10.1139/m91-157

subject

Has Abstract

pub_date

1991-12-01 00:00:00

pages

908-11

issue

12

eissn

0008-4166

issn

1480-3275

journal_volume

37

pub_type

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