Abstract：
:Fungal chitinases play essential roles in chitin degradation, cell wall remodeling, chitin recycling, nutrition acquisition, autolysis, and virulence. In this study, 18 genes of the glycoside hydrolase 18 (GH18) family were identified in the Isaria cicadae genome. Seventeen of the genes belonged to chitinases and one was an endo-β-N-acetylglucosaminidase (ENGase). According to phylogenetic analysis, the 17 chitinases were designated as subgroups A (7 chitinases), B (7), and C (3). The exon-intron organizations of these genes were analyzed. The conserved regions DxxDxDxE and S/AxGG and the domains CBM1, CBM18, and CBM50 were detected in I. cicadae chitinases and ENGase. The results of analysis of expression patterns showed that genes ICchiA1, ICchiA6, ICchiB1, and ICchiB4 had high transcript levels in the different growth conditions or developmental stages. Subgroup A chitinase genes had higher transcript levels than the genes of all other chitinases. Subgroup B chitinase genes (except ICchiB7) presented higher transcript levels in chitin medium compared with other conditions. ICchiC2 and ICchiC3 were mainly transcribed in autolysis medium and in blastospores, respectively. Moreover, ICchiB1 presented higher transcript levels than genes of other chitinases. This work provides an overview of the GH18 chitinases and ENGase in I. cicadae and provides a context for the chitinolytic potential, functions, and biological controls of these enzymes of entomopathogenic fungi.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Peng Y,Wang L,Gao Y,Ye L,Xu H,Li S,Jiang J,Li G,Dang Xdoi
10.1139/cjm-2019-0129subject
Has Abstractpub_date
2020-04-01 00:00:00pages
274-287issue
4eissn
0008-4166issn
1480-3275journal_volume
66pub_type
杂志文章abstract：:Grapes inoculated with poliovirus 1 and coxsackievirus B5 were washed with water, 0.5% polyehtylene glycol, or phosphate-buffered saline with 1% serum. These washes were equally efficient at removing virus but much of the virus in the water was noninfectious until treated with 0.5% polyethylene glycol. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-195
更新日期：1977-09-01 00:00:00
abstract：:When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests incre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W08-035
更新日期：2008-06-01 00:00:00
abstract：:Fecal coliforms (FC) counts were compared with Escherichia coli counts in differently contaminated freshwater samples (n = 166). FC were enumerated by plate count on triphenyl 2,3,5-tetrazolium chloride Tergitol medium. Escherichia coli were enumerated by the most probable number microplate method based on the detecti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-033
更新日期：2007-06-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-204
更新日期：1975-09-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-052
更新日期：2007-08-01 00:00:00
abstract：:When Caryophanon latum was exposed to egg white lysozyme in isotonic sucrose and observed by phase-contrast microscopy, protoplasts emerged along the length of the trichomes, apparently at sites corresponding to cross septa. Electron microscopy of sections revealed that this enzyme initially attacked the core of the s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-024
更新日期：1975-02-01 00:00:00
abstract：:Raw milk samples from bulk tanks in Ontario were analyzed for the presence of Listeria species. The overall incidence of Listeria species in raw milk was 12.4%. Listeria innocua was most frequently isolated and was found in 9.7% (43/445) of the raw milk samples, while L. monocytogenes and L. welshimeri were each found...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-020
更新日期：1988-02-01 00:00:00
abstract：:Escherichia coli B and E. aurescens, Shigella alkalescens, and Proteus vulgaris et P. morganii tryptophanases (TPases) were studied for the spectral forms of the enzyme. The pH effect on the absorption spectrum and on the enzyme specific activity revealed that the coli group TPases are identical with but differ from P...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-06-01 00:00:00
abstract：:Cultures of Streptomyces peucetius often were observed to divert spontaneously from the production of anthracycline antibiotics to the excretion of acidic metabolites, whether grown in a defined medium or a nutrient-rich medium. The predominant excreted acidic metabolites were determined by high-performance liquid chr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-198
更新日期：1987-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0630
更新日期：2015-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0708
更新日期：2014-01-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-069
更新日期：2003-09-01 00:00:00
abstract：:Frankia are gram-positive, filamentous bacteria capable of fixing atmospheric dinitrogen in symbiosis with a wide variety of woody plants and shrubs. Some isolates of Frankia harbor plasmids of 8.5 (pFQ11) and 22.4 kb (pFQ12) that have no known function but are transmitted through many generations in culture. We have ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-050
更新日期：2001-07-01 00:00:00
abstract：:Mice immunized subcutaneously with 10(6) viable yeast cells of Histoplasma capsulatum exhibited host protection to a challenge infection and also exhibited concomitant manifestations of cellular immunity to a culture filtrate antigen (yeast extract dialysate (YED)-histoplasmin). Protective immunity was determined by q...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-016
更新日期：1983-01-01 00:00:00
abstract：:Growth of Neisseria perflava, Neisseria cinerea, and Neisseria sicca strain Kirkland in media supplemented with sucrose (0.5 to 5.0% w/v) resulted in the formation of giant cells. Response to sucrose was specific in that a variety of other carbohydrates did not mediate giant cell formation. Giant cells appeared only u...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-065
更新日期：1976-03-01 00:00:00
abstract：:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent micr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-063
更新日期：1979-03-01 00:00:00
abstract：:The biochemical reactions, carbohydrate content, and 16S-rRNA sequences of Tatlockia (Legionella) maceachernii and Tatlockia micdadei strains were studied. Except for catalase activity, Tatlockia strains were relatively inert in the biochemical tests commonly used in clinical laboratories. Phenotypically, the two Tatl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-069
更新日期：1993-05-01 00:00:00
abstract：:Farnesol, a quorum sensing (QS) signal, is produced by Candida albicans during high density growth and has been found to inhibit morphogenesis. This QS auto-inducing signal was discovered to increase amino acid incorporation by C. albicans when concentrations of farnesol increased to 10 microg/mL in yeast nitrogen bro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-050
更新日期：2005-08-01 00:00:00
abstract：:This paper reports two different enterotoxigenic classes of the genus Aeromonas isolated from fishes. Strains of A. sobria were isolated from healthy fishes only wheras strains of A. hydrophila were isolated from both healthy and moribund fishes. Aeromonas sobria and hydrophilia strains produced a cytotoxic factor and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-174
更新日期：1977-09-01 00:00:00
abstract：:Menadione reductase (EC 1.6.99.2) has been purified 67-fold from Clostridium tyrobutyricum extracts. The molecular weight was found to be 60 000 and the prosthetic group was identified as FMN on the basis of the enzymatic analysis data. The binding of FMN to the menadione dehydrogenase apoenzyme was relatively weak wi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-053
更新日期：1980-03-01 00:00:00
abstract：:Canadian oil sands tailings are predominately sodic residues contaminated by hydrocarbons such as naphthenic acids. These conditions are harsh for plant development. In this study, we evaluated the effect of inoculating roots of Alnus viridis ssp. crispa and Alnus incana ssp. rugosa with ectomycorrhizal fungi in the p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0703
更新日期：2016-07-01 00:00:00
abstract：:Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/m95-091
更新日期：1995-08-01 00:00:00
abstract：:The immune benefits associated with the optimal intake of dietary fatty acids are widely known. The objective of the present investigation was to elucidate the role of omega-3 polyunsaturated fatty acids (n-3 PUFA) food source on acute pneumonia induced by Klebsiella pneumoniae. Three different n-3 PUFA preparations (...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0521
更新日期：2013-07-01 00:00:00
abstract：:The induction of antituberculous immunity by crude ribosomal fractions isolated from Mycobacterium tuberculosis strain H37Ra, M. bovis strain BCG, and M. smegmatis was studied in CF-1 mice. Levels of antituberculous immunity similar to that induced by live BCG were induced by the BCG and H37Ra ribosomal fractions wher...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-032
更新日期：1983-02-01 00:00:00
abstract：:The physiological characteristics and fatty acid composition of 40 strains of Yarrowia lipolytica isolated from irradiated poultry meat, commercial chilled foods, the superficial water of lagoons of an Italian river delta, and commercial light butter were compared. Discriminant analysis of the results permitted the 40...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-008
更新日期：1994-01-01 00:00:00
abstract：:Seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (IA and IB), two on day 4 (IVA and IVB), one on day 8 (VIIIA), and two on day 9 (IXA and IXB). The lambs were reared in these isolators until ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-064
更新日期：1989-03-01 00:00:00
abstract：:Fetal bovine serum (FBS) is a necessary constituent of the culture media employed to foster the growth of Trypanosoma cruzi epimastigote forms. In different laboratories, the serum is used at final concentrations of 5 or 10%. We have normally supplemented the complex medium with 10% FBS. Under this condition we have d...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-132
更新日期：1995-10-01 00:00:00
abstract：:Brachiaria, predominantly an African genus, contains species, such as B. brizantha, an apomictic C4 grass, that are commercially important forage grasses in tropical America, where they now cover about 55 million hectares. From B. brizantha accession CIAT 6780, we isolated an endophytic fungus that may be economically...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-127
更新日期：2001-01-01 00:00:00
abstract：:The course of intranasal infection of Sendai virus in CBA and DBA mice was investigated in animals decomplemented with purified cobra venom factor. The mice were decomplemented either immediately before inoculation or at 4 days postinfection. Depletion of complement after the infection had been established had no appa...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-072
更新日期：1982-05-01 00:00:00
abstract：:Intestinal infections are a major cause of mortality in domestic rabbits. Although a few pathogenic organisms have been associated with this type of infection, it is often impossible to demonstrate the presence of any etiologic agents. Recently viral particles were observed in the intestine of diseased rabbits submitt...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-201
更新日期：1980-10-01 00:00:00
