Proteolytic activity of Oidiodendron kalrai.

Abstract:

:The physiochemical characteristics of the intracellular proteolytic enzymes of Oidiodendron kalari, a neuropathogenic fungus, were studied. The organism in the yeast phase was grown in a semisynthetic medium containing 1% tryptone, at 37 degrees C for 48 hr, on a gyrotory shaker. The crude extract was prepared by breaking the cells in a French pressure cell and the proteolytci activity was tested against biological substrates. The cell-free extract hydrolyzed casein, hemoglobin, lactalbumin, gelatin, elastin, collagen and purified rabbit renal basement membrane to various degrees. Optimal proteolytic activity was observed at pH 6 and at 32 degrees C. Calcium and EDTA did not affect the enzymatic activity; however, activity was partially inhibited by sulfhydryl-blocking agents and by heat-inactivated horse, calf, and human serum. The extract was totally inactivated by exposure to a temperature of 70 degrees C for 60 min. Storage at -76 degrees C or -15 degrees C for 6 months or at 4 degrees C for 4 weeks did not affect protease activity.

journal_name

Can J Microbiol

authors

Cino PM,Tewari RP

doi

10.1139/m75-204

subject

Has Abstract

pub_date

1975-09-01 00:00:00

pages

1362-8

issue

9

eissn

0008-4166

issn

1480-3275

journal_volume

21

pub_type

杂志文章
  • The presence of Listeria spp. in raw milk in Ontario.

    abstract::Raw milk samples from bulk tanks in Ontario were analyzed for the presence of Listeria species. The overall incidence of Listeria species in raw milk was 12.4%. Listeria innocua was most frequently isolated and was found in 9.7% (43/445) of the raw milk samples, while L. monocytogenes and L. welshimeri were each found...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-020

    authors: Farber JM,Sanders GW,Malcolm SA

    更新日期:1988-02-01 00:00:00

  • Isolation and PCR amplification of a species-specific oxidoreductase-coding gene region in Listeria grayi.

    abstract::Listeria grayi is a nonpathogenic Gram-positive bacterium that demonstrates considerable similarities to other members in the genus Listeria, including the foodborne human pathogen Listeria monocytogenes and the animal pathogen Listeria ivanovii. A rapid diagnostic test to identify and diagnose listeriosis would be va...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-108

    authors: Liu D,Lawrence ML,Ainsworth AJ,Austin FW

    更新日期:2005-01-01 00:00:00

  • Amino acid uptake systems in Bacteroides ruminicola.

    abstract::Uptake of amino acids by Bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. A high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. All amino acids except proline we...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-180

    authors: Stevenson RM

    更新日期:1979-10-01 00:00:00

  • Carotenoid profiles of yeasts belonging to the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus.

    abstract::Eighteen yeast species of the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus, each one represented by its type strain, were investigated with the objective of evaluating their carotenoid composition. The pigments were extracted from yeast cells, quantified by high pressure liquid chromatography ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W07-068

    authors: Buzzini P,Innocenti M,Turchetti B,Libkind D,van Broock M,Mulinacci N

    更新日期:2007-08-01 00:00:00

  • New evidence for an intermediate polymer of glucose in cellulose biosynthesis by Acetobacter xylinum.

    abstract::The results of sucrose-density-gradient centrifugation of a cell-free particulate enzyme system from Acetobacter xylinum which was incubated with uridine diphosphoglucose indicate that there is a polymeric intermediate in the biosynthesis of cellulose. This intermediate has the properties of an oligomer of glucose, is...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-018

    authors: Kjosbakken J,Colvin JR

    更新日期:1975-02-01 00:00:00

  • Purification and properties of mercuric reductase from Yersinia enterocolitica 138A14.

    abstract::A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m93-027

    authors: Blaghen M,Vidon DJ,el Kebbaj MS

    更新日期:1993-02-01 00:00:00

  • Molecular identification of a polyM-specific alginate lyase from Pseudomonas sp. strain KS-408 for degradation of glycosidic linkages between two mannuronates or mannuronate and guluronate in alginate.

    abstract::An alginate lyase gene of a newly isolated Pseudomonas sp. strain KS-408 was cloned by using PCR with the specific primers designed from homologous nucleotide sequences. A partial protein sequence of KS-408 alginate lyase was homology-modeled on the basis of the crystal structure of A1-III alginate lyase from Sphingom...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w11-106

    authors: Kam N,Park YJ,Lee EY,Kim HS

    更新日期:2011-12-01 00:00:00

  • Intrinsic limitations on the continued production of the antibiotic patulin by Penicillium urticae.

    abstract::The initial cause of the cessation of patulin biosynthesis in submerged cultures of Penicillium urticae was sought. The extrinsic limitations to patulin biosynthesis in fermentor cultures were successfully removed and kinetic profiles of the metabolites of the pathway and of three pathway enzymes were determined over ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m81-032

    authors: Neway J,Gaucher GM

    更新日期:1981-02-01 00:00:00

  • Assessing and analysing contamination of a dairy products processing plant by Staphylococcus aureus using antibiotic resistance and PFGE.

    abstract::A dairy product processing plant was studied for 2.5 years to examine contamination with Staphylococcus aureus and try to correlate the source of contamination. Cultures were submitted to an antibiotic susceptibility test (AST) and characterised by Pulsed-field Gel Electrophoresis (PFGE) analysis. Results showed that ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w00-111

    authors: Tondo EC,Guimarães MC,Henriques JA,Ayub MA

    更新日期:2000-12-01 00:00:00

  • Immunovirological studies on human respiratory syncytial virus structural proteins.

    abstract::Immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the Paramyxoviridae family: the two external membrane glycoproteins H (90 000) and Fo (F1, 49 000; F2, 20 000; disulfide linked), the internal membrane protein M (34 000), t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-004

    authors: Trudel M,Nadon F,Séguin C,Ghoubril S,Payment P,Trépanier P

    更新日期:1986-01-01 00:00:00

  • Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution.

    abstract::Antibiotic susceptibility tests and restriction enzyme analysis (REA) of genomic DNA were performed to characterize the relationship between sources of isolates of yellow-pigmented enterococci. Antibiotic susceptibility tests were conducted with 10 therapeutic antibiotics and 54 isolates grouped by source (wild and ot...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-44-11-1066

    authors: Bahirathan M,Puente L,Seyfried P

    更新日期:1998-11-01 00:00:00

  • Microbial degradation of nitrobenzene and mono-nitrophenol by bacteria enriched from municipal activated sludge.

    abstract::Using a mixture of three mono nitrophenols as sole carbon, nitrogen and energy sources, mixed cultures were enriched from municipal activated sludge to degrade both nitrophenols and nitrobenzene. Bacterial growth and degradation rate could be increased by supplementing the medium with 0.1% YE. Microorganisms were isol...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Zhao JS,Ward OP

    更新日期:1999-05-01 00:00:00

  • Complete genome sequence of Paracoccus denitrificans ATCC 19367 and its denitrification characteristics.

    abstract::Studies show that Paracoccus denitrificans can denitrify nitrogen sources under aerobic conditions. However, the lack of data on its genome sequence has restricted molecular studies and practical applications. In this study, the complete genome of P. denitrificans ATCC 19367 was sequenced and its nitrogen metabolism p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2019-0037

    authors: Si YY,Xu KH,Yu XY,Wang MF,Chen XH

    更新日期:2019-07-01 00:00:00

  • Molecular and morphological markers for rapid distinction between 2 Colletotrichum species.

    abstract::Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, ho...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w09-059

    authors: Pileggi SA,Vieira de Oliveira SF,Andrade CW,Vicente VA,Dalzoto Pdo R,Kniphoff da Cruz G,Gabardo J,Massola NS,Tozze HJ,Pileggi M,Kava-Cordeiro V,Galli-Terasawa LV,Pimentel IC,Stringari D,Glienke C

    更新日期:2009-09-01 00:00:00

  • Bacillus subtilis SL-13 biochar formulation promotes pepper plant growth and soil improvement.

    abstract::The use of microbial fertilizers can help to avoid the harmful effects of traditional agricultural fertilizers and pesticides; however, there are many constraints on the practical application of such fertilizers. In this study, microbial biochar formulations (MBFs) were obtained by loading biochar, created from agricu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2018-0333

    authors: Tao S,Wu Z,Wei M,Liu X,He Y,Ye BC

    更新日期:2019-05-01 00:00:00

  • Attempts to inhibit ruminal methanogenesis by blocking pyruvate oxidative decarboxylation.

    abstract::The inhibition of pyruvate oxidative decarboxylation as a means of decreasing ruminal methanogenesis in vitro was studied. In the first experiment, the addition of adenosine and adenine (with and without ribose) to ruminal batch cultures did not decrease methanogenesis. In the second experiment, the addition of oxythi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-079

    authors: Ungerfeld EM,Rust SR,Burnett R

    更新日期:2003-10-01 00:00:00

  • Nutrition and phylogeny of predacious yeasts.

    abstract::Yeast predation was studied with respect to the range of its distribution among ascomycetous yeasts, the range of yeast species that can be affected, and nutritional aspects of the phenomenon. The yeasts identified as predators belong to the Saccharomycopsis clade as defined on the basis of rDNA sequence relatedness. ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w00-021

    authors: Lachance MA,Pupovac-Velikonja A,Natarajan S,Schlag-Edler B

    更新日期:2000-06-01 00:00:00

  • Spatial distribution of bacterial communities in sediment of a eutrophic lake revealed by denaturing gradient gel electrophoresis and multivariate analysis.

    abstract::Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the results were interpreted ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W08-098

    authors: Zeng J,Yang LY,Liang Y,Li JY,Xiao L,Jiang LJ,Zhao DY

    更新日期:2008-12-01 00:00:00

  • Aggregation during sexual development in Dictyostelium discoideum.

    abstract::A microcinematographic analysis of the behaviour and movements of cells and cell masses in mated cultures (NC4 X VI2) of Dictyostelium discoideum indicates that a chemotactic process directs cell aggregation during macrocyst development. Zygote giant cells form before aggregation begins and act as the aggregation cent...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-221

    authors: O'Day DH

    更新日期:1979-12-01 00:00:00

  • Comparison of methods for the concentration of Cryptosporidium oocysts and Giardia cysts from raw waters.

    abstract::This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration-elution methods: flatbed membranes, FiltaMax foam, Envirochek HV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-059

    authors: Ferguson C,Kaucner C,Krogh M,Deere D,Warnecke M

    更新日期:2004-09-01 00:00:00

  • Detection of Bacillus cereus virulence factors in commercial products of Bacillus thuringiensis and expression of diarrheal enterotoxins in a target insect.

    abstract::We examined isolates from 4 commercial bioinsecticides based on different strains of Bacillus thuringiensis subspecies (kurstaki, israelensis, aizawai, and tenebrionis) for the presence of genes encoding proteins with known enterotoxigenicity (nhe, hbl, cytk, ces) and various other putative virulence genes (piplc, sph...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W07-106

    authors: Kyei-Poku G,Gauthier D,Pang A,van Frankenhuyzen K

    更新日期:2007-12-01 00:00:00

  • Prophage-mediated production of a bacteriocinlike substance by SP beta lysogens of Bacillus subtilis.

    abstract::Cultures of Bacillus subtilis lysogenic for the temperature bacteriophage SP beta release "betacin", a bacteriocinlike substance that inhibits B. subtilis strains which do not carry this phage. Production of betacin is blocked by mutations in the bet gene on the prophage and a second phage gene, tol, is apparently inv...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-220

    authors: Hemphill HE,Gage I,Zahler SA,Korman RZ

    更新日期:1980-11-01 00:00:00

  • Improved antiserum agar method for the serogroup differentiation of Neisseria meningitidis Y and W135.

    abstract::Rabbit antisera prepared to meningococcal serogroups Y and W135 strains were compared with horse antisera using the antiserum agar method (ASA) for the serogroup identification of Neisseria meningitidis. Thirty-seven group Y strains formed immunoprecipitates with the Y rabbit serum only, whereas the same Y strains for...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-109

    authors: Ashton FE,Ryan A,Diena BB

    更新日期:1980-05-01 00:00:00

  • Evidence for conserved tRNA genes in the 16S-23S rDNA spacer sequence and two rrn operons of Xylella fastidiosa.

    abstract::The 16S-23S rDNA spacer of the type strain (ATCC 35879) of Xylella fastidiosa was amplified by PCR, cloned, and sequenced. The spacer sequence (455 bp) contains two tRNA (tRNA(ala) and tRNA(ile)) genes. Identical tRNA genes were also found in the 16S-23S spacer sequences of all the 51 strains of X. fastidiosa retrieve...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w00-114

    authors: Chen J,Banks D,Jarret RL,Jones JB

    更新日期:2000-12-01 00:00:00

  • Cyst and germ tube wall structure in Aphanomyces astaci, Oomycetes.

    abstract::Aphanomyces astaci secondary cyst walls and walls of germinating spores were prepared by alkaline hydrolysis, autolysis, sonication, and enzymic degradation and were examined by shadow-casting and negative-staining techniques. The cyst wall consists of randomly oriented fibrils, about 3 nm in diameter. The fibrils are...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-210

    authors: Nyhlén L,Unestam T

    更新日期:1978-11-01 00:00:00

  • Microbial surfactant activities from a petrochemical landfarm in a humid tropical region of Brazil.

    abstract::The goal of this study was to assess the presence and surfactant potential of naturally occurring microbes from a tropical soil with petrochemical contamination. Microorganisms in a soil sample from a Brazilian landfarm were isolated and grown on petroleum as the sole carbon source. Of 60 isolates screened for petrole...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W07-052

    authors: Maciel BM,Dias JC,Dos Santos AC,Filho RC,Fontana R,Loguercio LL,Rezende RP

    更新日期:2007-08-01 00:00:00

  • [Presence of Pseudomonas maltophilia in the rhizosphere of several cultivated plants].

    abstract::Pseudomonas maltophilia has been identified in the rhizosphere of several cultured plants: cabbage, rape, mustard, corn, beet. In comparison with nonrhizospheric soil, all the rhizospheric samples analysed contain more of this Pseudomonas and the most important stimulation is supplied by cruciferous plants. The possib...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Debette J,Blondeau R

    更新日期:1980-04-01 00:00:00

  • Immunological studies on staphylococcal enterotoxin D: production of murine monoclonal antibodies and immunopurification.

    abstract::Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m91-099

    authors: Shinagawa K,Omoe K,Matsusaka N,Sugii S

    更新日期:1991-08-01 00:00:00

  • Aerobic purification of N5,N10-methylenetetrahydromethanopterin dehydrogenase, separated from N5,N10-methylenetetrahydromethanopterin cyclohydrolase, from Methanobacterium thermoautotrophicum strain Marburg.

    abstract::The N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum strain Marburg has been purified with reasonable yield and much higher specific activity than previously reported. For the first time it has been shown that both N5,N10-methylenetetrahydromethanopterin dehydrogenase and...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-077

    authors: Mukhopadhyay B,Daniels L

    更新日期:1989-04-01 00:00:00

  • Aminoglycoside resistance mechanisms in Pseudomonas aeruginosa isolates from non-cystic fibrosis patients in Thailand.

    abstract::This study aimed to examine aminoglycosides (AMGs) resistance mechanisms, including the AMG-modifying enzyme genes, mexXY, rplY, nuoG, and galU, in the Pseudomonas aeruginosa non-cystic fibrosis (CF) isolates in Thailand. One hundred P. aeruginosa isolates from non-CF patients were examined for susceptibility to AMGs ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2012-0465

    authors: Poonsuk K,Tribuddharat C,Chuanchuen R

    更新日期:2013-01-01 00:00:00