Abstract:
:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent microscopic technique. The number of cells thus obtained is proposed as a direct cound of viable bacterial cells (DVC). With open ocean samples, DVC was higher than 'viable' plate counts by up to three orders of magnitude and lower than the direct counts by about one order.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Kogure K,Simidu U,Taga Ndoi
10.1139/m79-063subject
Has Abstractpub_date
1979-03-01 00:00:00pages
415-20issue
3eissn
0008-4166issn
1480-3275journal_volume
25pub_type
杂志文章abstract::Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2020-0239
更新日期:2021-01-01 00:00:00
abstract::The study investigated the presence of Borrelia-specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG pos...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-105
更新日期:2007-12-01 00:00:00
abstract::Environmentally compatible control measures are needed for suppression of Phytophthora capsici on pepper. Twenty-three isolates of Trichoderma were screened for suppression of a mixture of 4 genetically distinct isolates of this pathogen on bell pepper (Capsicum anuum) in greenhouse pot assays. Of these 23 isolates, G...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-071
更新日期:2010-10-01 00:00:00
abstract::In this study, 16S rRNA gene primers were designed to complement the suite of already available PCR primers for the detection of different methanogens involved in biogas production through anaerobic digestion by SYBR Green real-time PCR. Primers designed for use in TaqMan real-time PCR for the organisms Methanosaeta, ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-157
更新日期:2009-05-01 00:00:00
abstract::The metabolism of cyclohexanecarboxylic acid by a bacterium, designated PRL W19, follows a pathway involving beta-oxidation of coenzyme A intermediates analogous to the classical oxidation of fatty acids. The organism appears to have the property for the constitutive metabolism of caproic acid, and cell extracts conta...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-141
更新日期:1978-07-01 00:00:00
abstract::Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, ho...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-059
更新日期:2009-09-01 00:00:00
abstract::A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-059
更新日期:1997-05-01 00:00:00
abstract::The effects of the Clostridium difficile toxin were examined in HeLa and mouse adrenal tumor (MAT) cells. Cytotoxicity was evaluated by vital dye exclusion and 51Cr release. In both HeLa and MAT cells, C. difficile toxin caused rounding of virtually 100% of cells. This rounding was distinguishable from rounding produc...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-009
更新日期:1982-01-01 00:00:00
abstract::The correlation between the occurrence of indicator organisms and salmonellae in the same sample homogenate of various classes of dried foods was studied. In the first phase of the work involving 5837 samples, 1943 contained fecal coliforms and 100 contained Salmonella. Fecal coliforms were not detected in 53 of the S...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-141
更新日期:1976-07-01 00:00:00
abstract::The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on beta-glucuronidase activity, using the fluorogenic substrat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-157
更新日期:1991-12-01 00:00:00
abstract::Ten strains of Caldariomyces fumago and related fungi were found to produce extracellular chloroperoxidase when grown on a glucose - malt extract medium. High enzyme levels and pigment production were observed for C. fumago ATCC 16373 and C. fumago CMI 89362. Removal of malt extract from the medium and the replacement...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-199
更新日期:1981-12-01 00:00:00
abstract::Three strains, Rhodotorula rubra, Rhodotorula glutinis, and Candida zeylanoides, isolated from fish, were tested for the expression of putative tissue colonization factors. All strains were able to bind collagen type I, fibronectin, and laminin to various degrees after growing on various solid and broth media, while t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-171
更新日期:1993-12-01 00:00:00
abstract::Conidia of Torula caligans (Batista & Upadhyay) M. B. Ellis comb.nov. and T. terrestris Misra were examined by transmission- and scanning-electron microscopy. Torula caligans produced four-celled conidia in which the central cells were distinctly larger than the basal and apical cells. Conidia of T. terrestris were 4-...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-279
更新日期:1975-12-01 00:00:00
abstract::Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal comple...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-085
更新日期:2005-07-01 00:00:00
abstract::A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-106
更新日期:1983-06-01 00:00:00
abstract::Many studies on quorum quenching focus on the discovery and characterization of novel acyl-homoserine lactonases (AHL-lactonases) because these enzymes could be used in the control of diseases caused by Gram-negative bacteria. The effects of quorum quenching are also remarkable in the performance of bacterial consorti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2016-0323
更新日期:2017-01-01 00:00:00
abstract::Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-227
更新日期:1976-10-01 00:00:00
abstract::We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-008
更新日期:1992-01-01 00:00:00
abstract::The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-120
更新日期:1979-07-01 00:00:00
abstract::Aspergillus caespitosus NRRL 5769 growing in broth containing small amounts of sitosterol produced substance(s) with greater inhibitory activity against Candida albicans ATCC 10231 than in broth without sitosterol. Subsequent isolation, purification, and structural elucidation yielded 5,6-dihydro-5(S)-acetoxy-6(S)-1,2...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-259
更新日期:1975-11-01 00:00:00
abstract::In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-186
更新日期:1988-09-01 00:00:00
abstract::Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Neisseria gonorrhoeae strains can be grouped or differentiated (auxotyped) by their requirements for none, or any one or more of proline, uracil, hypoxanthine, and citrulline or ornithine. Most strains were readily assessed because they responded with growth or no growth on each defined auxanographic medium. Other str...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-204
更新日期:1983-10-01 00:00:00
abstract::The peptidoglycan of Escherichia coli K-12 strain AB264 was isolated by treating whole cells with sodium dodecyl sulfate and was purified by deoxyribonuclease, ribonuclease, and trypsin treatment. Like the peptidoglycan of Bacillus subtilis, this peptidoglycan proved able to bind substantial amounts of metallic ions f...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-031
更新日期:1984-02-01 00:00:00
abstract::A mesophilic anaerobe, a member of the Bacteroidaceae family (NRC2248), isolated from a cellulose-enrichment culture, digested untreated Whatman cellulose powder and HCl-treated cotton battings while producing hydrogen, carbon dioxide, cellobiose, glucose, and acetic acid as the sole volatile acid. This organism also ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-222
更新日期:1979-12-01 00:00:00
abstract::Aphanomyces astaci secondary cyst walls and walls of germinating spores were prepared by alkaline hydrolysis, autolysis, sonication, and enzymic degradation and were examined by shadow-casting and negative-staining techniques. The cyst wall consists of randomly oriented fibrils, about 3 nm in diameter. The fibrils are...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-210
更新日期:1978-11-01 00:00:00
abstract::The phototrophic purple sulfur bacterium Thiopedia rosea forms multicellular, gas-vacuolate, regular, flat aggregates (platelets, sheets) held together by slime. Platelets found in eutrophic water consisted of slime (85% of the total wet volume) and 16 cells, while the gas-filled vacuole occupied 44% of the volume of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-113
更新日期:1986-07-01 00:00:00
abstract::The accumulation of radioactive Cs by fungi was studied by analysis of fruit bodies (n (total) = 205, n greater than or equal to 5 for 22 species) collected in 1988 in a Norwegian mountain area with high deposition of radiocaesium from the Chernobyl accident. To account for site variation, the radiocaesium content of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-119
更新日期:1990-10-01 00:00:00
abstract::The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-033
更新日期:1992-03-01 00:00:00
abstract::Yeast predation was studied with respect to the range of its distribution among ascomycetous yeasts, the range of yeast species that can be affected, and nutritional aspects of the phenomenon. The yeasts identified as predators belong to the Saccharomycopsis clade as defined on the basis of rDNA sequence relatedness. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-021
更新日期:2000-06-01 00:00:00