A tentative direct microscopic method for counting living marine bacteria.

Abstract:

:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent microscopic technique. The number of cells thus obtained is proposed as a direct cound of viable bacterial cells (DVC). With open ocean samples, DVC was higher than 'viable' plate counts by up to three orders of magnitude and lower than the direct counts by about one order.

journal_name

Can J Microbiol

authors

Kogure K,Simidu U,Taga N

doi

10.1139/m79-063

subject

Has Abstract

pub_date

1979-03-01 00:00:00

pages

415-20

issue

3

eissn

0008-4166

issn

1480-3275

journal_volume

25

pub_type

杂志文章
  • Exposure of Agrobacterium tumefaciens to agroinfiltration medium demonstrates cellular remodelling and may promote enhanced adaptability for molecular pharming.

    abstract::Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2020-0239

    authors: Prudhomme N,Pastora R,Muselius B,McLean MD,Cossar D,Geddes-McAlister J

    更新日期:2021-01-01 00:00:00

  • Immune complexes in early Lyme disease.

    abstract::The study investigated the presence of Borrelia-specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG pos...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W07-105

    authors: Lencáková D,Stefancíková A,Ivanová R,Petko B

    更新日期:2007-12-01 00:00:00

  • Selection of genetically diverse Trichoderma spp. isolates for suppression of Phytophthora capsici on bell pepper.

    abstract::Environmentally compatible control measures are needed for suppression of Phytophthora capsici on pepper. Twenty-three isolates of Trichoderma were screened for suppression of a mixture of 4 genetically distinct isolates of this pathogen on bell pepper (Capsicum anuum) in greenhouse pot assays. Of these 23 isolates, G...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-071

    authors: Roberts DP,Maul JE,McKenna LF,Emche SE,Meyer SL,Collins RT,Bowers JH

    更新日期:2010-10-01 00:00:00

  • Design and testing of real-time PCR primers for the quantification of Methanoculleus, Methanosarcina, Methanothermobacter, and a group of uncultured methanogens.

    abstract::In this study, 16S rRNA gene primers were designed to complement the suite of already available PCR primers for the detection of different methanogens involved in biogas production through anaerobic digestion by SYBR Green real-time PCR. Primers designed for use in TaqMan real-time PCR for the organisms Methanosaeta, ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-157

    authors: Franke-Whittle IH,Goberna M,Insam H

    更新日期:2009-05-01 00:00:00

  • The microbial degradation of cyclohexanecarboxylic acid by a beta-oxidation pathway with simultaneous induction to the utilization of benzoate.

    abstract::The metabolism of cyclohexanecarboxylic acid by a bacterium, designated PRL W19, follows a pathway involving beta-oxidation of coenzyme A intermediates analogous to the classical oxidation of fatty acids. The organism appears to have the property for the constitutive metabolism of caproic acid, and cell extracts conta...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-141

    authors: Blakley ER

    更新日期:1978-07-01 00:00:00

  • Molecular and morphological markers for rapid distinction between 2 Colletotrichum species.

    abstract::Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, ho...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w09-059

    authors: Pileggi SA,Vieira de Oliveira SF,Andrade CW,Vicente VA,Dalzoto Pdo R,Kniphoff da Cruz G,Gabardo J,Massola NS,Tozze HJ,Pileggi M,Kava-Cordeiro V,Galli-Terasawa LV,Pimentel IC,Stringari D,Glienke C

    更新日期:2009-09-01 00:00:00

  • Polygalacturonase isolated from the culture of the psychrophilic fungus Sclerotinia borealis.

    abstract::A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m97-059

    authors: Takasawa T,Sagisaka K,Yagi K,Uchiyama K,Aoki A,Takaoka K,Yamamato K

    更新日期:1997-05-01 00:00:00

  • Morphologic and functional effects of Clostridium difficile enterotoxin in tissue culture.

    abstract::The effects of the Clostridium difficile toxin were examined in HeLa and mouse adrenal tumor (MAT) cells. Cytotoxicity was evaluated by vital dye exclusion and 51Cr release. In both HeLa and MAT cells, C. difficile toxin caused rounding of virtually 100% of cells. This rounding was distinguishable from rounding produc...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m82-009

    authors: Gurwith MJ,Langston C,Dunsmore B

    更新日期:1982-01-01 00:00:00

  • ICMSF methods studies. VII. Indicator tests as substitutes for direct testing of dried foods and feeds for Salmonella.

    abstract::The correlation between the occurrence of indicator organisms and salmonellae in the same sample homogenate of various classes of dried foods was studied. In the first phase of the work involving 5837 samples, 1943 contained fecal coliforms and 100 contained Salmonella. Fecal coliforms were not detected in 53 of the S...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-141

    authors: Silliker JH,Gabis DA

    更新日期:1976-07-01 00:00:00

  • Evaluation of beta-glucuronidase assay for the detection of Escherichia coli from environmental waters.

    abstract::The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on beta-glucuronidase activity, using the fluorogenic substrat...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m91-157

    authors: Shadix LC,Rice EW

    更新日期:1991-12-01 00:00:00

  • A defined growth medium for the production of chloroperoxidase by Caldariomyces fumago.

    abstract::Ten strains of Caldariomyces fumago and related fungi were found to produce extracellular chloroperoxidase when grown on a glucose - malt extract medium. High enzyme levels and pigment production were observed for C. fumago ATCC 16373 and C. fumago CMI 89362. Removal of malt extract from the medium and the replacement...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m81-199

    authors: Pickard MA

    更新日期:1981-12-01 00:00:00

  • The expression of potential colonization factors of yeasts isolated from fish during different growth conditions.

    abstract::Three strains, Rhodotorula rubra, Rhodotorula glutinis, and Candida zeylanoides, isolated from fish, were tested for the expression of putative tissue colonization factors. All strains were able to bind collagen type I, fibronectin, and laminin to various degrees after growing on various solid and broth media, while t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m93-171

    authors: Vázquez-Juárez R,Ascencio F,Andlid T,Gustafsson L,Wadström T

    更新日期:1993-12-01 00:00:00

  • The fine structure of conidial development in the genus Torula. II. T. caligans (Batista and Upadhyay) M. B. Ellis and T. terrestris Mistra.

    abstract::Conidia of Torula caligans (Batista & Upadhyay) M. B. Ellis comb.nov. and T. terrestris Misra were examined by transmission- and scanning-electron microscopy. Torula caligans produced four-celled conidia in which the central cells were distinctly larger than the basal and apical cells. Conidia of T. terrestris were 4-...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-279

    authors: Ellis DH,Griffiths DA

    更新日期:1975-12-01 00:00:00

  • Antigenic and genetic characterization of serogroup C meningococci isolated from invasive meningococcal disease cases in Canada from 1999 to 2003.

    abstract::Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal comple...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-085

    authors: Law DK,Stoltz J,Henderson AM,Tsang RS

    更新日期:2005-07-01 00:00:00

  • A technique for preserving aerial fungal structures for scanning electron microscopy.

    abstract::A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-106

    authors: King EJ,Brown MF

    更新日期:1983-06-01 00:00:00

  • Evidence-based validation of quorum quenching from Lysinibacillus sphaericus and Geobacillus sp. in bioremediation of oil sludge.

    abstract::Many studies on quorum quenching focus on the discovery and characterization of novel acyl-homoserine lactonases (AHL-lactonases) because these enzymes could be used in the control of diseases caused by Gram-negative bacteria. The effects of quorum quenching are also remarkable in the performance of bacterial consorti...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2016-0323

    authors: Gómez-Garzón C,Dussán J

    更新日期:2017-01-01 00:00:00

  • Leakage of periplasmic enzymes from lipopolysaccharide-defective mutants of Salmonella typhimurium.

    abstract::Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-227

    authors: Chatterjee AK,Ross H,Sanderson KE

    更新日期:1976-10-01 00:00:00

  • Induction of C4-dicarboxylate transport genes by external stimuli in Rhizobium meliloti.

    abstract::We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-008

    authors: Batista S,Castro S,Aguilar OM,Martínez-Drets G

    更新日期:1992-01-01 00:00:00

  • Relationship between carbon source and susceptibility of Cephalosporium acremonium to selected amino acid analogues.

    abstract::The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-120

    authors: Mehta RJ,Nash CH

    更新日期:1979-07-01 00:00:00

  • Three antimicrobial metabolites from Aspergillus caespitosus.

    abstract::Aspergillus caespitosus NRRL 5769 growing in broth containing small amounts of sitosterol produced substance(s) with greater inhibitory activity against Candida albicans ATCC 10231 than in broth without sitosterol. Subsequent isolation, purification, and structural elucidation yielded 5,6-dihydro-5(S)-acetoxy-6(S)-1,2...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-259

    authors: Mizuba S,Lee K,Jiu J

    更新日期:1975-11-01 00:00:00

  • Discriminant analysis of microcalorimetric data of bacterial growth.

    abstract::In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-186

    authors: Bermúdez J,López D,Sanahuja A,Viñas M,Lorén JG

    更新日期:1988-09-01 00:00:00

  • Polyclonal antibodies against fusaproliferin.

    abstract::Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Monti SM,Fogliano V,Randazzo G,Peluso G,Logrieco A,Ritieni A

    更新日期:1999-01-01 00:00:00

  • Growth responses of Neisseria gonorrhoeae auxotypes to required amino acids and bases in liquid medium.

    abstract::Neisseria gonorrhoeae strains can be grouped or differentiated (auxotyped) by their requirements for none, or any one or more of proline, uracil, hypoxanthine, and citrulline or ornithine. Most strains were readily assessed because they responded with growth or no growth on each defined auxanographic medium. Other str...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-204

    authors: Hendry AT

    更新日期:1983-10-01 00:00:00

  • Metal binding by the peptidoglycan sacculus of Escherichia coli K-12.

    abstract::The peptidoglycan of Escherichia coli K-12 strain AB264 was isolated by treating whole cells with sodium dodecyl sulfate and was purified by deoxyribonuclease, ribonuclease, and trypsin treatment. Like the peptidoglycan of Bacillus subtilis, this peptidoglycan proved able to bind substantial amounts of metallic ions f...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m84-031

    authors: Hoyle BD,Beveridge TJ

    更新日期:1984-02-01 00:00:00

  • Cellulose degradation by a new isolate from sewage sludge, a member of the Bacteroidaceae family.

    abstract::A mesophilic anaerobe, a member of the Bacteroidaceae family (NRC2248), isolated from a cellulose-enrichment culture, digested untreated Whatman cellulose powder and HCl-treated cotton battings while producing hydrogen, carbon dioxide, cellobiose, glucose, and acetic acid as the sole volatile acid. This organism also ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-222

    authors: Saddler JN,Khan AW

    更新日期:1979-12-01 00:00:00

  • Cyst and germ tube wall structure in Aphanomyces astaci, Oomycetes.

    abstract::Aphanomyces astaci secondary cyst walls and walls of germinating spores were prepared by alkaline hydrolysis, autolysis, sonication, and enzymic degradation and were examined by shadow-casting and negative-staining techniques. The cyst wall consists of randomly oriented fibrils, about 3 nm in diameter. The fibrils are...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-210

    authors: Nyhlén L,Unestam T

    更新日期:1978-11-01 00:00:00

  • Structure, partial elemental composition, and size of Thiopedia rosea cells and platelets.

    abstract::The phototrophic purple sulfur bacterium Thiopedia rosea forms multicellular, gas-vacuolate, regular, flat aggregates (platelets, sheets) held together by slime. Platelets found in eutrophic water consisted of slime (85% of the total wet volume) and 16 cells, while the gas-filled vacuole occupied 44% of the volume of ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-113

    authors: Scherrer R,Shull VE

    更新日期:1986-07-01 00:00:00

  • Accumulation of radiocaesium in fungi.

    abstract::The accumulation of radioactive Cs by fungi was studied by analysis of fruit bodies (n (total) = 205, n greater than or equal to 5 for 22 species) collected in 1988 in a Norwegian mountain area with high deposition of radiocaesium from the Chernobyl accident. To account for site variation, the radiocaesium content of ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m90-119

    authors: Bakken LR,Olsen RA

    更新日期:1990-10-01 00:00:00

  • Nucleotide sequence analysis of the gene encoding the Caulobacter crescentus paracrystalline surface layer protein.

    abstract::The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-033

    authors: Gilchrist A,Fisher JA,Smit J

    更新日期:1992-03-01 00:00:00

  • Nutrition and phylogeny of predacious yeasts.

    abstract::Yeast predation was studied with respect to the range of its distribution among ascomycetous yeasts, the range of yeast species that can be affected, and nutritional aspects of the phenomenon. The yeasts identified as predators belong to the Saccharomycopsis clade as defined on the basis of rDNA sequence relatedness. ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w00-021

    authors: Lachance MA,Pupovac-Velikonja A,Natarajan S,Schlag-Edler B

    更新日期:2000-06-01 00:00:00