Abstract:
:A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate detergent. For the whey permeate medium used, the optimum LA:detergent ratio was 1:15 (w/w). As a result, the cultures tolerated at least 1000 microg x mL(-1) LA, which was converted to CLA with 57%-87% efficiency. The cis-9, trans-11 and trans-9, cis-11 isomers constituted 85%-90% of the CLA produced. The feasibility of the method was demonstrated also in de Man Rogosa-Sharpe (MRS) broth.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Rainio A,Vahvaselkä M,Suomalainen T,Laakso Ssubject
Has Abstractpub_date
2001-08-01 00:00:00pages
735-40issue
8eissn
0008-4166issn
1480-3275journal_volume
47pub_type
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