Abstract:
:The Bacillus subtilis glutamyl-tRNA synthetase (GluRS), encoded by the gltX gene, aminoacylates its homologous tRNA(Glu) and tRNA(Gln) with glutamate. This gene was cloned with its sigma A promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and B. subtilis. Transformation of B. subtilis with this recombinant plasmid (pMP411) led to a 30-fold increase of glutamyl-tRNA synthetase specific activity in crude extracts. Transformation of E. coli with this plasmid gave no recombinants, but transformation with plasmids bearing an altered gltX was successful. These results indicate that the presence of B. subtilis glutamyl-tRNA synthetase is lethal for E. coli, probably because this enzyme glutamylates tRNA1(Gln) in vivo as it does in vitro.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Pelchat M,Lacoste L,Yang F,Lapointe Jsubject
Has Abstractpub_date
1998-04-01 00:00:00pages
378-81issue
4eissn
0008-4166issn
1480-3275journal_volume
44pub_type
杂志文章abstract::The use of microbial fertilizers can help to avoid the harmful effects of traditional agricultural fertilizers and pesticides; however, there are many constraints on the practical application of such fertilizers. In this study, microbial biochar formulations (MBFs) were obtained by loading biochar, created from agricu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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