Abstract:
:We examined isolates from 4 commercial bioinsecticides based on different strains of Bacillus thuringiensis subspecies (kurstaki, israelensis, aizawai, and tenebrionis) for the presence of genes encoding proteins with known enterotoxigenicity (nhe, hbl, cytk, ces) and various other putative virulence genes (piplc, sph, bceT, entFM, entS, entT). The piplc and bceT sequences were present in all the isolates; sph was found in aizawai and israelensis; entFM only in israelensis; and entS in kurstaki, israelensis, and tenebrionis. Our results corroborate previous findings that isolates used in commercial products contain all nhe and hbl component genes but not the ces gene. We ascertained that the cytK gene present in the kurstaki-, israelensis-, and aizawai-based products belongs to the cytK-2 type and not the more toxigenic cytK-1 variant originally isolated from enterotoxic Bacillus cereus. We provide the first evidence that hemolytic (hblA) and nonhemolytic (nheA, nheB, nheC) enterotoxin genes are expressed during septicemia in a target insect. This opens the door for their possible participation in pathogenesis in target insects. If enterotoxins do not contribute to bacterial pathogenesis in target insects, their genes could be deleted from commercial production strains to pre-empt perceptions of public health risks.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Kyei-Poku G,Gauthier D,Pang A,van Frankenhuyzen Kdoi
10.1139/W07-106subject
Has Abstractpub_date
2007-12-01 00:00:00pages
1283-90issue
12eissn
0008-4166issn
1480-3275pii
w07-106journal_volume
53pub_type
杂志文章abstract::The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cyto...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0606
更新日期:2013-06-01 00:00:00
abstract::An intracellular invertase was induced in cultures of Clostridium pasteurianum utilizing sucrose as its carbon source for growth. This enzyme synthesis could be repressed by the addition of fructose of a sucrose-growing culture. In contrast, invertase activity was not affected by the addition of glucose to sucrose-gro...
journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-249
更新日期:1977-12-01 00:00:00
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abstract::Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obta...
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journal_title:Canadian journal of microbiology
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更新日期:1977-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-065
更新日期:1976-03-01 00:00:00
abstract::Through genomic analysis of mucosa-associated Escherichia coli strains, we found a close genetic association among isolates from pediatric inflammatory bowel disease (IBD) patients. A specific E. coli pathovar, adherent-invasive E. coli (AIEC), was found in Crohn's disease (CD) adult patients - this pathovar has enhan...
journal_title:Canadian journal of microbiology
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doi:10.1139/w2012-007
更新日期:2012-04-01 00:00:00
abstract::The release of 14CO2 from 14C-labelled glucose(G-1(-14)C, G-3,4(-14)C, G-6(-14)C) was followed in phased cultures of Candida utilis grown in a glucose- mineral salts medium under altered conditions of carbon:nitrogen limitation at doubling times of 2,4 and 6, h. Changes in oxygen uptake and CO2 evolution were observe...
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更新日期:2005-10-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-073
更新日期:1976-04-01 00:00:00
abstract::Trace gas uptake by microorganisms controls the oxidative capacity of the troposphere, but little is known about how this important function is affected by changes in soil microbial diversity. This article bridges that knowledge gap by examining the response of the microbial community-level physiological profiles (CLP...
journal_title:Canadian journal of microbiology
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abstract::Uptake of amino acids by Bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. A high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. All amino acids except proline we...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1979-10-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2013-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1975-11-01 00:00:00
abstract::Clinical isolates of Staphylococcus aureus were found to exhibit strain-specific heterogeneity to the growth-enhancing effects of human urokinase (UK), a proteinase with plasminogen activator activity. Nine out of fourteen (64%) methicillin-sensitive strains of S. aureus were responsive to UK in "in vitro" cultures. I...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1996-10-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. A...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
abstract::Enzymes induced by two Rhizobium trifolii bacteriophages caused depolymerization of exopolysaccharides from most R. trifolii and R. leguminosarum strains tested, but did not, in general, attack the exopolysaccharides of R. meliloti, the slow-growing rhizobia, or Agrobacterium. Ca2+ and (or) Mg2+ were required for enzy...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1975-10-01 00:00:00
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更新日期:2001-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2000-04-01 00:00:00
abstract::Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
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更新日期:1995-08-01 00:00:00