Abstract:
:A tryptophan auxotroph of aerobically grown Rodospirillum rubrum was isolated after mutagenesis and replica plating. The mutant grows on tryptophan or indole, accumulates indole glycerol phosphate, lacks the capacity to convert indole glycerol phosphate to indole glycerol phosphate to indole, and finally is defective in photosynthetic growth. The strain is, therefore, analogous to a Trp A mutant which is defective in the alpha-subunit structural gene of tryptophan synthetase.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Beuchemin-Newhouse N,Cedergen RJdoi
10.1139/m78-055subject
Has Abstractpub_date
1978-03-01 00:00:00pages
330-2issue
3eissn
0008-4166issn
1480-3275journal_volume
24pub_type
杂志文章abstract::In this study, 16S rRNA gene primers were designed to complement the suite of already available PCR primers for the detection of different methanogens involved in biogas production through anaerobic digestion by SYBR Green real-time PCR. Primers designed for use in TaqMan real-time PCR for the organisms Methanosaeta, ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-157
更新日期:2009-05-01 00:00:00
abstract::This paper reports two different enterotoxigenic classes of the genus Aeromonas isolated from fishes. Strains of A. sobria were isolated from healthy fishes only wheras strains of A. hydrophila were isolated from both healthy and moribund fishes. Aeromonas sobria and hydrophilia strains produced a cytotoxic factor and...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-174
更新日期:1977-09-01 00:00:00
abstract::To screen, isolate, and characterize bacterial populations producing microcin J25, we report here two rapid, reliable, and sensitive methods, using polymerase chain reaction and colony blot hybridization with a digoxigenin-labelled probe. A sample of 26 Escherichia coli strains isolated from poultry intestinal content...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::The accumulation of radioactive Cs by fungi was studied by analysis of fruit bodies (n (total) = 205, n greater than or equal to 5 for 22 species) collected in 1988 in a Norwegian mountain area with high deposition of radiocaesium from the Chernobyl accident. To account for site variation, the radiocaesium content of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-119
更新日期:1990-10-01 00:00:00
abstract::Cellulomonas fimi genomic DNA was digested with HpaI, MunI, HindIII, and NsiI, producing fragments ranging in size from 20 to 1400 kbp that were resolved by pulsed field gel electrophoresis. Genetic and physical linkages were determined by Southern blotting and were used to construct a genome map. Cellulomonas fimi ha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks. We used pulsed-fi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-058
更新日期:1993-04-01 00:00:00
abstract::Fifty-four strains of Neisseria gonorrhoeae were examined for their ability to be agglutinated by various lectins. Wheat germ agglutinin, ricin, soybean lectin, and peanut agglutinin agglutinated all strains tested. Dolichos biflorus, Sophora japonica, Maclura pomifera, Ulex Europaeus, Lens culinaris, Canavalia ensifo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-078
更新日期:1980-04-01 00:00:00
abstract::The catabolism of glucose by Streptomyces C5, a producer of anthracycline antibiotics, was investigated to determine the pathways that supply precursors for anthracycline biosynthesis. Carbons for the biosynthesis of epsilon-rhodomycinone, an anthracycline aglycone, from radiolabelled glucose were derived primarily fr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-217
更新日期:1988-11-01 00:00:00
abstract::The purpose of this study was to determine the prevalence of antibiotic-resistant bacteria and endotoxin in soil after land application of biosolids. Soil was collected over a 15 month period following land application of biosolids, and antibiotic resistance was ascertained using clinically relevant antibiotic concent...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-021
更新日期:2007-05-01 00:00:00
abstract::The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cyto...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0606
更新日期:2013-06-01 00:00:00
abstract::A Tn551 insertional pleiotropic mutant defective in the production of several exoproteins was isolated from Staphylococcus aureus 196E and characterized. The pleiotropism of the mutant was due to a single insertion of the transposon as evidenced by Southern blot hybridization and by the transfer of its phenotype by tr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-107
更新日期:1994-08-01 00:00:00
abstract::Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-227
更新日期:1976-10-01 00:00:00
abstract::Studies show that Paracoccus denitrificans can denitrify nitrogen sources under aerobic conditions. However, the lack of data on its genome sequence has restricted molecular studies and practical applications. In this study, the complete genome of P. denitrificans ATCC 19367 was sequenced and its nitrogen metabolism p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0037
更新日期:2019-07-01 00:00:00
abstract::In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-186
更新日期:1988-09-01 00:00:00
abstract::We present preliminary data on the role of antibiotics in intraabdominal sepsis using a new, clinically relevant animal model. Peritoneal cavity infection was induced by ligation and perforation of the cecum in adult rats. Surviving rats were randomized to receive either saline or cefoxitin at the time of cecal excisi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-059
更新日期:1988-03-01 00:00:00
abstract::Incubation of 4'-chloro-4-biphenylol with a mushroom tyrosinase preparation gave the catechol, 4'-chloro-3,4-biphenyldiol as the sole in vitro metabolite. This compound was identical with the major rat urinary metabolite of 4'-chloro-4-biphenylol and thus confirms the structure assigned to the metabolite. This result ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-014
更新日期:1976-01-01 00:00:00
abstract::Enzymes induced by two Rhizobium trifolii bacteriophages caused depolymerization of exopolysaccharides from most R. trifolii and R. leguminosarum strains tested, but did not, in general, attack the exopolysaccharides of R. meliloti, the slow-growing rhizobia, or Agrobacterium. Ca2+ and (or) Mg2+ were required for enzy...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-239
更新日期:1975-10-01 00:00:00
abstract::The 40-kDa porin protein of Haemophilus influenzae type b was reconstituted into proteoliposomes. The relative rates of diffusion of small uncharged sugars across the channels formed by this protein were determined by measuring the rates of osmotic swelling of the liposomes. From these rates, a pore diameter of 1.8 nm...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-027
更新日期:1988-02-01 00:00:00
abstract::The 16S-23S rDNA spacer of the type strain (ATCC 35879) of Xylella fastidiosa was amplified by PCR, cloned, and sequenced. The spacer sequence (455 bp) contains two tRNA (tRNA(ala) and tRNA(ile)) genes. Identical tRNA genes were also found in the 16S-23S spacer sequences of all the 51 strains of X. fastidiosa retrieve...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-114
更新日期:2000-12-01 00:00:00
abstract::Sera from mice which have been vaccinated with BCG and challenged with old tuberculin contain gamma interferon. These same sera also express antibacterial activity. Using Staphylococcus aureus we demonstrated that its growth was inhibited at dilutions of sera as high as 1:320. A 4% concentration of sera reduced the gr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-084
更新日期:1986-05-01 00:00:00
abstract::Concentric bodies were found in vegetative and pycnidial cells of Podoxythium tricothecium and in ascostromal paraphyses of Rhytidhysterium rufulum, two representatives of the Loculoascomycetes. They averages 185 nm in diameter in P. trichothecium and 255 nm in R. rufulum and consisted of three zones. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-218
更新日期:1977-10-01 00:00:00
abstract::Mycoplasma pneumoniae is an important causative agent of atypical pneumonia. This study was to determine the ability of a DNA expression vector, which encodes the carboxy terminal region of the M. pneumoniae P1 protein (P1C), to induce humoral and cellular immune responses and to protect against M. pneumoniae infecti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-041
更新日期:2012-05-01 00:00:00
abstract::Frankia are gram-positive, filamentous bacteria capable of fixing atmospheric dinitrogen in symbiosis with a wide variety of woody plants and shrubs. Some isolates of Frankia harbor plasmids of 8.5 (pFQ11) and 22.4 kb (pFQ12) that have no known function but are transmitted through many generations in culture. We have ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-050
更新日期:2001-07-01 00:00:00
abstract::An Escherichia coli K-12 biofilm was grown at a dilution rate of 0.028 h(-1) for 48 h in a glucose-limited chemostat coupled to a modified Robbins' device to determine its susceptibility to infection by bacteriophage T4. Bacteriophage T4 at a multiplicity of infection (MOI) of 10 caused a log reduction in biofilm dens...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
abstract::Auxotrophs in three different races of Pyricularia oryzae were obtained following ultraviolet mutagenesis, and tested for complementarity on minimal medium. Prototrophic growth resulted between combinations of different auxotrophs of single strains, but not between strains. The growth did not result form crossfeeding,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-079
更新日期:1976-04-01 00:00:00
abstract::A real-time polymerase chain reaction assay that uses degenerate primers and a dual-labelled probe was developed to detect the bexA gene of Haemophilus influenzae, including those belonging to non-b serotypes as well as clonal division II strains. This assay is sensitive and specific, detecting 20 copies of the gene, ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0040
更新日期:2013-05-01 00:00:00
abstract::Environmental factors influencing the occurrence and community structure of soil yeasts in forests are not well studied. There are few studies dedicated to Southern Hemisphere soil yeasts populations and even fewer focused on temperate forests influenced by volcanic activity. The present work aimed to study the ecolog...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0897
更新日期:2014-06-01 00:00:00
abstract::The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-033
更新日期:1992-03-01 00:00:00
abstract::Nodulation of Pisum sativum cv. Afghanistan by Rhizobium leguminosarum strain Tom can be blocked by the nonnodulating R. leguminosarum strain PF2. This system was used to study the dynamics of rhizobial binding to seedling root surfaces. Appreciable numbers of 35S-labeled rhizobia bound to the root surface within 2h a...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-099
更新日期:1980-04-01 00:00:00
abstract::A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w99-055
更新日期:1999-08-01 00:00:00