Abstract：
:Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. Although many studies have examined the association between in vitro biofilm formation and isolates from a specific infection type, few have made comparisons from isolates from a broad range of isolates grouped by clinical source. In our study 50 NTHi from different clinical sources, otitis media, conjunctivitis, lower respiratory tract infections in both cystic fibrosis and non-cystic fibrosis patients, and nasopharyngeal carriage, plus 10 nasopharyngeal isolates of the commensal Haemophilus haemolyticus were tested for the ability to form biofilm by using a static microtitre plate crystal violet assay. A high degree of variation in biofilm forming ability was observed across all isolates, with no statistically significant differences observed between the groups, with the exception of the isolates from conjunctivitis. These isolates had uniformly lower biofilm forming ability compared with isolates from the other groups (p < 0.005).
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Obaid NA,Jacobson GA,Tristram Sdoi
10.1139/cjm-2014-0763subject
Has Abstractpub_date
2015-03-01 00:00:00pages
243-5issue
3eissn
0008-4166issn
1480-3275journal_volume
61pub_type
杂志文章abstract：:Through genomic analysis of mucosa-associated Escherichia coli strains, we found a close genetic association among isolates from pediatric inflammatory bowel disease (IBD) patients. A specific E. coli pathovar, adherent-invasive E. coli (AIEC), was found in Crohn's disease (CD) adult patients - this pathovar has enhan...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-007
更新日期：2012-04-01 00:00:00
abstract：:Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-051
更新日期：1982-03-01 00:00:00
abstract：:A quantitative and qualitative survey of the indigenous Endogone population in wheat field soil and vesicular-arbuscular mycorrhiza in wheat roots as influenced by season and by the stage of development of the wheat plant was made. The number of Endogone spores in wheat field soil remained relatively unchanged in win...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-151
更新日期：1975-07-01 00:00:00
abstract：:Sera from mice which have been vaccinated with BCG and challenged with old tuberculin contain gamma interferon. These same sera also express antibacterial activity. Using Staphylococcus aureus we demonstrated that its growth was inhibited at dilutions of sera as high as 1:320. A 4% concentration of sera reduced the gr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-084
更新日期：1986-05-01 00:00:00
abstract：:Phialocephala fortinii is a dark septate fungal endophyte that colonizes roots of many host species. Its effect on plant growth varies from being pathogenic to beneficial. The basic biology of this species has received little research, and thus the main objectives of this study were to determine cytological features o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-018
更新日期：2006-07-01 00:00:00
abstract：:The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0770
更新日期：2016-07-01 00:00:00
abstract：:Enterococci, formerly confounded with faecal streptococci, are recognized since the beginning of the century as being faecal in origin and are generally searched for in waste waters and food products; their detection may in fact indicate the presence of enteropathogenic organisms. Although nearly ubiquitous, their pre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for st...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-245
更新日期：1976-11-01 00:00:00
abstract：:Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The initial cause of the cessation of patulin biosynthesis in submerged cultures of Penicillium urticae was sought. The extrinsic limitations to patulin biosynthesis in fermentor cultures were successfully removed and kinetic profiles of the metabolites of the pathway and of three pathway enzymes were determined over ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-032
更新日期：1981-02-01 00:00:00
abstract：:Campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. No adhesins specific for C. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. Scanning electron microscopy revealed C. fetus attachment to the serosal surface...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w07-115
更新日期：2008-01-01 00:00:00
abstract：:There are inherent weaknesses associated with currently used bacterial fecal pollution indicator systems. Fecal pollution indicator data would be more meaningful if supplemented with information relating to the occurrence of pathogens in recreational water. Through surveys of four bathing beaches on Lake Ontario, it w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-159
更新日期：1979-09-01 00:00:00
abstract：:Cell surface hydrophobicity influences adhesion and virulence of the opportunistic fungal pathogen Candida albicans. Previous studies have shown that cell surface hydrophobicity is due to specific proteins that are exposed on hydrophobic cells but are masked by long fibrils on hydrophilic cells. This observation sugge...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-043
更新日期：1994-04-01 00:00:00
abstract：:The microbial community plays an important role in soil nutrient cycles and energy transformations in alpine grassland. In this study, we investigated the composition of the soil microbial community collected from alpine cold swamp meadow (ASM), alpine cold meadow (AM), and alpine cold desert steppe (ADS) within the B...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2017-0585
更新日期：2018-03-01 00:00:00
abstract：:Products obtained from the action of arylamine synthetase on [G-14C]chorismic acid were fractionated by gel filtration and ion exchange column chromatography to yield a partially purified radioactive component with an arylamine function. From its ultraviolet absorption spectrum and thin-layer chromatographic behaviour...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-032
更新日期：1976-02-01 00:00:00
abstract：:Various soil fungi were tested for their capacity to degrade the insecticide [14C]photodieldrin. Of nine species investigated, Trichoderma viride was the only one which degraded the insecticide to an appreciable extent into water-soluble, non-insecticidal compounds within 4-5 weeks. These products amounted to 32-41% o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-198
更新日期：1976-09-01 00:00:00
abstract：:One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20-18, a rifa...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-029
更新日期：2001-05-01 00:00:00
abstract：:Spiroplasmas were cultured from nonsurface-sterilized flowers of magnolia tree (Magnolia grandiflora L.) and tulip tree (Liriodendron tulipfera L.) in Alameda, Solano, and Yolo counties in California. Spiroplasmas were also isolated from honey bees (Apis mellifera L.) collected in the vicinity of Davis and Berkeley, C...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-038
更新日期：1981-02-01 00:00:00
abstract：:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent micr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-063
更新日期：1979-03-01 00:00:00
abstract：:Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-209
更新日期：1983-10-01 00:00:00
abstract：:Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-099
更新日期：1991-08-01 00:00:00
abstract：:Shiga toxins produced by Escherichia coli O157:H7 cause a wide spectrum of enteric diseases, such as lethal hemorrhagic colitis and hemolytic uremic syndrome. In this study, the B subunit protein of Shiga toxin type 1 (Stx1) was produced in the E. coli system, was further purified by Ni-column Affinity Chromatography ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W10-087
更新日期：2010-12-01 00:00:00
abstract：:Nonmotile and motility-altered mutants of Myxococcus xanthus have been obtained by the use of chemical mutagens, ultraviolet irradiation, and a procedure for selective spontaneous mutants. As judged by their behaviour on a variety of growth media, in both plate and slide culture, the mutants were divided into four gro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-190
更新日期：1976-09-01 00:00:00
abstract：:Fermentation properties under the control of multiple genes are difficult to alter with traditional methods in Saccharomyces cerevisiae. Here, a novel genome engineering approach is developed to improve ethanol production in very high gravity fermentation with 300 g/L glucose as the carbon source. This strategy involv...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-032
更新日期：2010-06-01 00:00:00
abstract：:Nonlinear survivor curves were obtained when spores of Bacillus cereus were heated in physiological saline solution. Curvilinear survivor curves did not appear to be caused by experimental artifacts but by the heterogeneity of spore population with regard to heat resistance. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-217
更新日期：1975-10-01 00:00:00
abstract：:Influenza undergoes extensive antigenic variation in nature. These new antigenic variants invariably supplant the preceding antigenic type that then disappears. In apparent contrast to this, two H3N2 strains that were forwarded to the Laboratory Centre for Disease Control, Ottawa, in 1984 from a Canadian public health...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-139
更新日期：1987-09-01 00:00:00
abstract：:A visual micromethod for measuring antifungal effects on germination and growth is described. The antifungal agent griseofulvin and the fungus Trichophyton mentagrophytes were used as materials to compare the micromethod with a standard assay based on dry mycelial weight. The micromethod was more sensitive than the we...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-093
更新日期：1978-05-01 00:00:00
abstract：:A test is described for selective inhibition of Mycobacterium tuberculosis and M. bovis isolates in fluid medium. The method employs rho-nitro-alpha-acetyl-amino-beta-hydroxy-propiophenone (NAP) as an inhibitory agent for differentiation of mammalian tuberculosis strains from other Mycobacteria. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-124
更新日期：1978-06-01 00:00:00
abstract：:Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal comple...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-085
更新日期：2005-07-01 00:00:00
abstract：:Group B streptococcus type III is a major cause of neonatal death. The terminal sialic acid moiety of the group B streptococcus type specific capsule has been shown to be an important virulence factor. We demonstrate here that bacteria grown in human serum have increased cell surface sialic acid content compared with ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-016
更新日期：1994-02-01 00:00:00
