Comparison of element levels in minimal and complex yeast media.

Abstract:

:The cellular functions are strongly influenced by the composition of the environment. In particular, phenotypes of microbial strains are modulated by concentrations of ions in the culture medium, and differences in element levels may be responsible for a phenotypic variability observed when microbial strains are grown on synthetic versus complex media. In this report, we analyzed the levels of nine elements (magnesium, potassium, sodium, calcium, iron, copper, manganese, zinc, and phosphorus) and sulphate ions in commercially available peptone and yeast extract and compared them with those in yeast nitrogen base routinely used for preparation of synthetic minimal media. We observed that whereas some elements are present at similar levels, the levels of others differ by a factor as high as 20. The observed differences should be taken into account when interpreting different phenotypes observed for microbial strains grown on synthetic versus complex media.

journal_name

Can J Microbiol

authors

Abelovska L,Bujdos M,Kubova J,Petrezselyova S,Nosek J,Tomaska L

doi

10.1139/W07-012

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

533-5

issue

4

eissn

0008-4166

issn

1480-3275

pii

w07-012

journal_volume

53

pub_type

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