Abstract：
:One of the important properties of probiotics is the ability to survive in the intestine. There have been few studies on the probiotic property of lactococci, since they are formally not considered to be natural inhabitants of the intestine. To evaluate lactococci as probiotic bacteria, we investigated their ability to survive during gastric transit by in vitro and in vivo tests. When exposed to an in vitro simulated gastrointestinal environment, such as low pH and bile, only Lactococcus lactis subsp. lactis bv. diacetylactis N7 showed a moderate survival rate among the four strains tested. The tested strains were orally administered to mice, and intestinal passage of the ingested strains was monitored by two methods: antibiotics and PCR. Viable cells of strain N7 were recovered from feces within 24-48 h after administration but not at 72 h. Lactococcus lactis subsp. cremoris ATCC 19257, which had a poor survival rate in vitro test, was also detected at 12 h but not at 24 h. These results indicate that lactococci can reach the mouse intestine alive, but not colonize it. If administered daily, viable strain N7 may exist continuously in the intestine. The effect of strain N7 on intestinal microbial balance and on animal health will be the subject of a further study.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Kimoto H,Nomura M,Kobayashi M,Mizumachi K,Okamoto Tdoi
10.1139/w03-092subject
Has Abstractpub_date
2003-11-01 00:00:00pages
707-11issue
11eissn
0008-4166issn
1480-3275pii
w03-092journal_volume
49pub_type
杂志文章abstract：:No clear data are available on how flavonoids from different chemical groups affect root colonization by arbuscular mycorrhizal fungi (AMF) and whether flavonoids affecting the presymbiotic growth of AMF also affect root colonization by AMF. In the present work, we compared the effect of flavones (chrysin and luteolin...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-036
更新日期：2007-06-01 00:00:00
abstract：:Cells of five Bacteroides species were examined following treatment with homologous antisera and staining with ruthenium red. They were enveloped by glycocalyces and these extensive fibrous exopolysaccharide matrices were fully retained as an integral "capsule" by some cells, while other cells showed "capsule" as well...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-124
更新日期：1984-06-01 00:00:00
abstract：:Verticillium fungicola, isolated from Agaricus bisporus (commercial mushroom), produced significant extracellular hydrophobin when grown for 7 days in a static liquid culture of synthetic minimal medium. The hydrophobin was purified by precipitation with ammonium sulphate (80% saturation), Sephadex G-100 gel filtratio...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-098
更新日期：2002-11-01 00:00:00
abstract：:Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-015
更新日期：1989-01-01 00:00:00
abstract：:A bacterial strain capable of utilizing 3,6-dimethylphenanthrene (3,6-DMP) as its sole source of carbon and energy was isolated from a creosote-contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS1. Utilization of 3,6-DMP was demonstrated by an increase in bacteria...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-018
更新日期：2003-02-01 00:00:00
abstract：:Microsclerotia of three melanin-deficient mutants of Verticillium dahliae formed malanin from (+)-scytalone, 1,8-dihydroxynaphthalene, catechol, and L-3,4-dihydroxyphenylalanine. The melanins formed from (+)-scytalone or 1,8-dihydroxynaphthalene resembled wild-type melanin chemically and ultrastructurally, whereas the...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-049
更新日期：1978-03-01 00:00:00
abstract：:Cultures of Sclerotium rolfsii and Trichoderma viride together in autoclaved soil were assayed at intervals during 8 days of incubation for proteolytic activity (PA) of T. viride. Significant proteolytic activity was detected only in soil containing T. viride (i.e., T. viride alone or S. rolfsii + T. viride); greatest...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-079
更新日期：1978-04-01 00:00:00
abstract：:Twenty-six strains of group JK corynebacteria had the same colonial morphology and biological reactions as the biotypes of the biovars of Corynebacterium genitalium and C. pseudogenitalium. Therefore, group JK corynebacteria can be assigned to the biovars of C. genitalium or C. pseudogenitalium. Although the strains d...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-164
更新日期：1984-08-01 00:00:00
abstract：:beta-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-146
更新日期：2009-04-01 00:00:00
abstract：:One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20-18, a rifa...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-029
更新日期：2001-05-01 00:00:00
abstract：:Marine shrimp of the species Penaeus monodon were collected from the coastal region (Haroa) of the deltaic Sundarbans of West Bengal, India during the premonsoon period in 1996. Klebsiella pneumoniae was isolated from the alimentary canal and gills of the shrimp as the sole isolate. All 10 isolated strains were resist...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:Acid (AcP) and alkaline phosphatase (AlP) were localized by physicobiochemical techniques. Greater than 53% of the phosphatases were detected, following sonication, in a low speed centrifugation pellet while osmotically shocked and spheroplasted Capnocytophaga species released only 9-28% and 11-43% of the cellular pho...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-212
更新日期：1983-10-01 00:00:00
abstract：:A simplified procedure is presented for releasing and concentrating indigenous microbial cells from soil for viewing by transmission electron microscopy as thin sections or replicas of frozen-etched preparations. This procedure is compared with two others reported earlier, and their relative merits are discussed as co...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-036
更新日期：1975-03-01 00:00:00
abstract：:The comparative ability of different methods to assess virulence of Listeria species was investigated in ten Listeria strains. All strains were initially subjected to pulsed-field gel electrophoresis analysis to determine their relatedness. Virulence characteristics were subsequently tested for by (i) determining the ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-129
更新日期：2006-04-01 00:00:00
abstract：:When Caryophanon latum was exposed to egg white lysozyme in isotonic sucrose and observed by phase-contrast microscopy, protoplasts emerged along the length of the trichomes, apparently at sites corresponding to cross septa. Electron microscopy of sections revealed that this enzyme initially attacked the core of the s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-024
更新日期：1975-02-01 00:00:00
abstract：:The initial cause of the cessation of patulin biosynthesis in submerged cultures of Penicillium urticae was sought. The extrinsic limitations to patulin biosynthesis in fermentor cultures were successfully removed and kinetic profiles of the metabolites of the pathway and of three pathway enzymes were determined over ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-032
更新日期：1981-02-01 00:00:00
abstract：:There is an increasing movement against use of antibiotic growth promoters in animal feed. Prebiotic supplementation is a potential alternative to enhance the host's natural defense through modulation of gut microbiota. In the present study, the effect of mannan oligosaccharide (MOS) and virginiamycin (VIRG) on cecal ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0899
更新日期：2014-05-01 00:00:00
abstract：:The monitoring of antimicrobial susceptibilities in Neisseria gonorrhoeae isolates and characterization of N. gonorrhoeae multiantigen sequence types (NG-MAST, ST) provide important surveillance data as resistance rates continue to rise. A total of 2970 N. gonorrhoeae isolates were collected by Canadian provincial pub...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0357
更新日期：2013-10-01 00:00:00
abstract：:In Salmonella typhimurium LT2 the F factor of Escherichia coli K-12 replicates normally but is repressed; Flac+ cells give no visible lysis on solid media with male-specific phages, low frequency transfer of Flac+ (0.001-0.007 per donor cell), few f2 infective centers (0.002-0.006 per cell), and they propagate male-sp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-184
更新日期：1983-09-01 00:00:00
abstract：:The role the environment has on populations of Pythium and Mesocriconema spp. was investigated at 5 golf course locations in east central Alabama. Every 4 to 5 weeks soil samples were collected from 3 golf greens on each of the 5 golf courses. Environmental data, including air and soil temperature, pH and relative hum...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-005
更新日期：2005-04-01 00:00:00
abstract：:Despite the interactions known to occur between various lower respiratory tract pathogens and alveolar epithelial cells (AECs), few reports examine factors influencing the interplay between Mycobacterium tuberculosis bacilli and AECs during infection. Importantly, in vitro studies have demonstrated that the M. tubercu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0528
更新日期：2015-12-01 00:00:00
abstract：:BaeR, a response regulator protein, takes part in multidrug efflux, bacterial virulence activity, and other biological functions. Recently, BaeR was shown to induce inflammatory responses by activating the mitogen-activated protein kinases (MAPKs). In this study, we investigated additional pathways used by BaeR to ind...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0057
更新日期：2016-09-01 00:00:00
abstract：:A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurosp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-147
更新日期：1991-11-01 00:00:00
abstract：:The introduction of the 7-valent pneumococcal vaccine (PCV7) in Canada was very effective in reducing invasive pneumococcal disease (IPD) in children; however, increases of non-PCV7 serotypes have subsequently offset some of these reductions. A 13-valent pneumococcal vaccine (PCV13) targeting additional serotypes was ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0614
更新日期：2013-12-01 00:00:00
abstract：:Plant secondary metabolites, such as saponins, have a considerable impact in agriculture because of their allelopathic effects. They also affect the growth of soil microorganisms, especially fungi. We investigated the influence of saponins on rhizosphere bacteria in vitro and in soil conditions. The effects of gypsoph...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-052
更新日期：2003-06-01 00:00:00
abstract：:Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. A...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0763
更新日期：2015-03-01 00:00:00
abstract：:Canadian oil sands tailings are predominately sodic residues contaminated by hydrocarbons such as naphthenic acids. These conditions are harsh for plant development. In this study, we evaluated the effect of inoculating roots of Alnus viridis ssp. crispa and Alnus incana ssp. rugosa with ectomycorrhizal fungi in the p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0703
更新日期：2016-07-01 00:00:00
abstract：:Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-021
更新日期：1989-01-01 00:00:00
abstract：:Iron and sulfur oxidation by Thiobacillus ferrooxidans as well as growth on ferrous iron were inhibited by a variety of low molecular weight organic compounds. The influences of chemical structure of the organic inhibitors, pH, temperature, physical treatment of cells, and added inhibitory or stimulatory inorganic ion...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-105
更新日期：1976-05-01 00:00:00
abstract：:Pathogenic and nonpathogenic isolates of Venturia inaequalis were grown in liquid culture. Hyphae were treated with two types of fimbrial antiserum (AU- and AV-1) and examined by immunofluorescent microscopy, in order to establish the distribution of fimbrial epitopes in whole cell mounts. The AV-1 antiserum was speci...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-061
更新日期：2000-09-01 00:00:00
