Abstract:
:Acid (AcP) and alkaline phosphatase (AlP) were localized by physicobiochemical techniques. Greater than 53% of the phosphatases were detected, following sonication, in a low speed centrifugation pellet while osmotically shocked and spheroplasted Capnocytophaga species released only 9-28% and 11-43% of the cellular phosphatases, respectively. French pressure cell disruption was more effective in releasing the phosphatases. Cell fragments were separated into cell wall, cytoplasmic membrane, and soluble fractions as determined by marker enzyme, chemical composition, transmission electron microscopy, sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE) protein analysis, and gel diffusion. AcP and AlP was partitioned between the isolated cell wall (31-46%) and soluble material (33-61%), with greater than 60% of the phosphatases remaining with the cell wall following Triton X-100 treatment. The amount of phosphatase at the surface of intact C. ochracea was quantitated by specific 125I-protein labelling.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Poirier TP,Holt SCdoi
10.1139/m83-212subject
Has Abstractpub_date
1983-10-01 00:00:00pages
1369-81issue
10eissn
0008-4166issn
1480-3275journal_volume
29pub_type
杂志文章abstract::Klebsiella pneumoniae was found to effect the reductive dechlorination of methoxychlor (2,2-bis(p-methoxyphenyl)-1, 1, 1-trichloroethane) and its dihydroxy derivative (2, 2-bis (p-hydroxyphenyl)-1, 1, 1-trichloroethane) to dichloro derivatives 2, 2-bis (p-methoxyphenyl)-1, 1-dichloroethane and 2, 2-bis (p-hydroxypheny...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-023
更新日期:1982-02-01 00:00:00
abstract::Trichloroethylene (TCE) is a prevalent contaminant of groundwater that can be cometabolically degraded by indigenous microbes. Groundwater contaminated with TCE from a US Department of Energy site in Ohio was used to characterize the site-specific impact of phenol on the indigenous bacterial community for use as a pos...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-025
更新日期:2005-06-01 00:00:00
abstract::Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/m95-091
更新日期:1995-08-01 00:00:00
abstract::The fine structure of conidia and immature resting spores of the entomogenous fungus Entomophthora apiculata is described. The two cell types contain comparable organelles and quantities of lipid, and possess a similar cell wall structure. The large quantity of stored lipid and numerous mitochondria in each cell type ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-067
更新日期:1977-04-01 00:00:00
abstract::Monoclonal antibodies reacting with the B subunit of Vibrio cholerae O1 strain 569B cholera toxin (CT-B) were used to identify unique and common epitopes of V. cholerae non-O1 and Vibrio mimicus CT-B. Vibrio cholerae non-O1 strains produced CT-B showing three monoclonal antibody reaction patterns (epitypes), which cor...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-071
更新日期:1990-06-01 00:00:00
abstract::The ability to rapidly identify and quantify a microbial strain in a complex environmental sample has widespread applications in ecology, epidemiology, and industry. In this study, we describe a rapid method to obtain functionally specific genetic markers that can be used in conjunction with standard or real-time poly...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-140
更新日期:2006-05-01 00:00:00
abstract::A species of Arthrobacter was recovered during culture of the causal organism of pitch canker of southern pines. Fusarium moniliforme var. subglutinans (FMS). Arthrobacter is a relatively common soil bacterium and is lytic to several fungal pathogens in the soil. Soil samples from two seed orchards with pitch canker a...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-004
更新日期:1981-01-01 00:00:00
abstract::The diversity of 16 strains of chickpea-infective rhizobia from various geographical origins was analysed using genotypic and phenotypic approaches. Multilocus enzyme electrophoresis was performed, and restriction fragment length polymorphisms of the amplified 16S + IGS (intergenic spacer) rRNA gene, assimilation of 1...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-057
更新日期:1994-05-01 00:00:00
abstract::This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjec...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-083
更新日期:2007-10-01 00:00:00
abstract::Group B streptococcus type III is a major cause of neonatal death. The terminal sialic acid moiety of the group B streptococcus type specific capsule has been shown to be an important virulence factor. We demonstrate here that bacteria grown in human serum have increased cell surface sialic acid content compared with ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-016
更新日期:1994-02-01 00:00:00
abstract::Mucor alternans(van Tieghem) was found to be a diphasic species in that it grew exclusively in the yeast-like budding phase under anaerobic conditions in the complex medium yeast extract-peptone-glucose broth and in tissue-culture medium 199. In the latter medium this growth form occurred also at 37C, at an initial pH...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-010
更新日期:1975-01-01 00:00:00
abstract::Shiga toxins produced by Escherichia coli O157:H7 cause a wide spectrum of enteric diseases, such as lethal hemorrhagic colitis and hemolytic uremic syndrome. In this study, the B subunit protein of Shiga toxin type 1 (Stx1) was produced in the E. coli system, was further purified by Ni-column Affinity Chromatography ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W10-087
更新日期:2010-12-01 00:00:00
abstract::The use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with approp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0261
更新日期:2020-01-01 00:00:00
abstract::Separation of Vero cell membrane in a discontinuous sucrose gradient reveals five fractions. After infection a sixth fraction appears. It contains virioins but mostly modified membranes with subunits 5-6 nm in diameter, probably the hemagglutinin. None of the enzymes used was associated with this fraction. No modifica...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-103
更新日期:1975-05-01 00:00:00
abstract::The 16S-23S rDNA spacer of the type strain (ATCC 35879) of Xylella fastidiosa was amplified by PCR, cloned, and sequenced. The spacer sequence (455 bp) contains two tRNA (tRNA(ala) and tRNA(ile)) genes. Identical tRNA genes were also found in the 16S-23S spacer sequences of all the 51 strains of X. fastidiosa retrieve...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-114
更新日期:2000-12-01 00:00:00
abstract::High resolution two-dimensional PAGE was used to analyse protein variation among serotype 1 poliovirus isolates. Viruses isolated from patients with recent histories of vaccination with live attenuated poliovirus were compared with prototype serotype 1 poliovaccine. The nonvaccine Mahoney and Brunenders strains of ser...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-136
更新日期:1988-06-01 00:00:00
abstract::The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-120
更新日期:1979-07-01 00:00:00
abstract::A Tn551 insertional pleiotropic mutant defective in the production of several exoproteins was isolated from Staphylococcus aureus 196E and characterized. The pleiotropism of the mutant was due to a single insertion of the transposon as evidenced by Southern blot hybridization and by the transfer of its phenotype by tr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-107
更新日期:1994-08-01 00:00:00
abstract::Verticillium fungicola, isolated from Agaricus bisporus (commercial mushroom), produced significant extracellular hydrophobin when grown for 7 days in a static liquid culture of synthetic minimal medium. The hydrophobin was purified by precipitation with ammonium sulphate (80% saturation), Sephadex G-100 gel filtratio...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-098
更新日期:2002-11-01 00:00:00
abstract::In mycetomes of leafhoppers, Helochara communis, ultrastructural and histochemical studies revealed that spheroplast symbiotes (t-symbiotes) were converted to 'a-symbiotes' (so-called), with apparent loss of DNA--a phenomenon reminiscent of rhizobium-bacteroid conversion in certain legume nodules. Additional t-symbiot...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-028
更新日期:1975-02-01 00:00:00
abstract::Transcriptional profiles of 2 unrelated clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were analyzed following 10% (v/v) ethanol challenge (15 min), which arrested growth but did not reduce viability. Ethanol-induced stress (EIS) resulted in differential gene expression of 1091 genes, 600 common ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2017-0221
更新日期:2017-09-01 00:00:00
abstract::The effects of postirradiation incubation with various chemicals structurally related to pantolactone on the survival of ultraviolet-irradiated Escherichia coli lon and ruv mutants were examined. Certain cyclic compounds with a butyrolactone, tetrahydrofuran, furan, tetrahydropyran, or 2-pyron ring showed substantial ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-254
更新日期:1980-12-01 00:00:00
abstract::The PII protein is apparently involved in the control of NifA activity in Herbaspirillum seropedicae. To evaluate the probable role of PII in signal transduction, uridylylation assays were conducted with purified H. seropedicae PII and Escherichia coli GlnD, or a cell-free extract of H. seropedicae as sources of uridy...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-018
更新日期:2001-04-01 00:00:00
abstract::To determine the ability of environmental bacteria to metabolize the frequently prescribed fluoroquinolone drug ciprofloxacin, eight Mycobacterium spp. cultures were grown for 4 days in a medium containing sorbitol and yeast extract with 100 mg x L(-1) ciprofloxacin. After the cultures had been centrifuged and the sup...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-101
更新日期:2007-01-01 00:00:00
abstract::No clear data are available on how flavonoids from different chemical groups affect root colonization by arbuscular mycorrhizal fungi (AMF) and whether flavonoids affecting the presymbiotic growth of AMF also affect root colonization by AMF. In the present work, we compared the effect of flavones (chrysin and luteolin...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-036
更新日期:2007-06-01 00:00:00
abstract::A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-151
更新日期:1977-08-01 00:00:00
abstract::A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-059
更新日期:1997-05-01 00:00:00
abstract::Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the results were interpreted ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W08-098
更新日期:2008-12-01 00:00:00
abstract::Pseudomonas maltophilia has been identified in the rhizosphere of several cultured plants: cabbage, rape, mustard, corn, beet. In comparison with nonrhizospheric soil, all the rhizospheric samples analysed contain more of this Pseudomonas and the most important stimulation is supplied by cruciferous plants. The possib...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::Histological examination of oesophagus and stomach specimens from 28 guinea pigs (Cavia porcellus) revealed microbes associated with the epithelial surface of these organs. Layers of Gram-positive cocci were associated with oesophageal tissue in all the animals. Yeasts (Torulop-sid pintolopesii) were associated with s...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-182
更新日期:1978-09-01 00:00:00