Abstract:
:Shiga toxins produced by Escherichia coli O157:H7 cause a wide spectrum of enteric diseases, such as lethal hemorrhagic colitis and hemolytic uremic syndrome. In this study, the B subunit protein of Shiga toxin type 1 (Stx1) was produced in the E. coli system, was further purified by Ni-column Affinity Chromatography method, and was then used as an immunogen to immunize laying hens for yolk immunoglobulin (IgY) production. Titers of IgY increased gradually with boosting vaccination and, finally, reached a level of 105, remaining steady over 1 year. Then the protective efficacy of IgY against Stx1 was evaluated by in vitro and in vivo experiments. It was shown that the anti-Stx1 IgY could effectively block the binding of Stx1 to the Hela cells and could protect BALB/c mice from toxin challenges. The data indicates the facility of using egg yolk IgY as a therapeutic intervention in cases of Shiga toxin intoxication.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Wang Q,Hou XJ,Cai K,Li T,Liu YN,Tu W,Xiao L,Bao SZ,Shi J,Gao X,Liu H,Tian RM,Wang Hdoi
10.1139/W10-087subject
Has Abstractpub_date
2010-12-01 00:00:00pages
1003-10issue
12eissn
0008-4166issn
1480-3275pii
w10-087journal_volume
56pub_type
杂志文章abstract::Pseudomonas maltophilia has been identified in the rhizosphere of several cultured plants: cabbage, rape, mustard, corn, beet. In comparison with nonrhizospheric soil, all the rhizospheric samples analysed contain more of this Pseudomonas and the most important stimulation is supplied by cruciferous plants. The possib...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-218
更新日期:1977-10-01 00:00:00
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-043
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
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