Abstract:
:The aim of the present study was to explore a new approach based on the hemagglutination (HA) assay to understand the colonization ability of Shigella spp. To study colonization ability, an animal model of 4-day-old suckling mouse, was exploited. We characterized the HA activity of 48 Shigella strains, with erythrocytes collected from rabbit, guinea pig, chicken, and sheep. Only rabbit and guinea pig erythrocytes showed positive HA reactions in most of the cases. On the basis of HA pattern, 4 strains from each serogroup were selected for in vivo colonization studies. Our results showed a positive correlation between HA activity and colonization ability of the strains belonging to different serogroups (groups A, B, C, and D) of Shigella. In all 4 serogroups, high HA titer was associated with greater intestinal colonization.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Mitra S,Saha DR,Pal A,Niyogi SK,Mitra U,Koley Hdoi
10.1139/w2012-095subject
Has Abstractpub_date
2012-10-01 00:00:00pages
1159-66issue
10eissn
0008-4166issn
1480-3275journal_volume
58pub_type
杂志文章abstract::We continued our study of high-molecular-mass proteases (HMMPs) using several strains of the genus Trichoderma, and other filamentous fungi (Botrytis cinerea, Aspergillus niger, Fusarium culmorum, and Penicillium purpurogenum). We found that five Trichoderma strains secreted HMMPs into the media after induction with b...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0670
更新日期:2019-09-01 00:00:00
abstract::Immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the Paramyxoviridae family: the two external membrane glycoproteins H (90 000) and Fo (F1, 49 000; F2, 20 000; disulfide linked), the internal membrane protein M (34 000), t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-004
更新日期:1986-01-01 00:00:00
abstract::Bacillus subtilis fmbR-1 was obtained from wild-type B. subtilis fmbR by replacement of the native promoter of the surfactin operon with the inducible promoter Pspac. The recombinant B. subtilis fmbR-1 produced more antibacterial substances than the wild-type strain. The overproducing phenotype was related to the enha...
journal_title:Canadian journal of microbiology
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doi:10.1139/w09-044
更新日期:2009-08-01 00:00:00
abstract::Listeria monocytogenes and Listeria innocua differ markedly in virulence but are indistinguishable by classical taxonomic criteria. Both species are actively motile and produce abundant flagellin at 22 degrees C. We have found, however, noticeable differences between L. monocytogenes and L. innocua in motility and fla...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-076
更新日期:1995-07-01 00:00:00
abstract::Brachiaria, predominantly an African genus, contains species, such as B. brizantha, an apomictic C4 grass, that are commercially important forage grasses in tropical America, where they now cover about 55 million hectares. From B. brizantha accession CIAT 6780, we isolated an endophytic fungus that may be economically...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-127
更新日期:2001-01-01 00:00:00
abstract::Mycoplasma pneumoniae is an important causative agent of atypical pneumonia. This study was to determine the ability of a DNA expression vector, which encodes the carboxy terminal region of the M. pneumoniae P1 protein (P1C), to induce humoral and cellular immune responses and to protect against M. pneumoniae infecti...
journal_title:Canadian journal of microbiology
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doi:10.1139/w2012-041
更新日期:2012-05-01 00:00:00
abstract::The aim of this study was to determine the effect of the rhizosphere of maize on the diversity of denitrifying bacteria. Community structure comparison was performed by constructing a collection of isolates recovered from bulk and maize planted soil. A total of 3240 nitrate-reducing isolates were obtained and 188 of t...
journal_title:Canadian journal of microbiology
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doi:10.1139/w04-037
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abstract::The regulation of de novo pyrimidine biosynthesis in Pseudomonas putida ATCC 17536 by pyrimidines was explored. The pathway enzyme activities were higher in glucose-grown cells than in succinate-grown cells, indicating catabolite repression by succinate. In P. putida cells grown on succinate as a carbon source, only a...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-110
更新日期:2002-12-01 00:00:00
abstract::The attachment of Bradyrhizobium sp. (Lupinus) strain MSDJ718 to excised roots of white lupin was examined. Maximal attachment occurred at early to middle log phases of bacterial growth. This binding was pH dependent, with an optimal value reached at 6.6. Irrespective of the culture age, the attachment was strongly af...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-035
更新日期:1996-03-01 00:00:00
abstract::The deliberate application of the methodology of contemporary molecular genetics to problems in bacterial systematics has led to a broad new understanding of the evolutionary history of both prokaryotes and eukaryotes. In this review, I discuss some of the major conclusions of this endeavour and try to predict future ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/m88-093
更新日期:1988-04-01 00:00:00
abstract::An Escherichia coli K-12 biofilm was grown at a dilution rate of 0.028 h(-1) for 48 h in a glucose-limited chemostat coupled to a modified Robbins' device to determine its susceptibility to infection by bacteriophage T4. Bacteriophage T4 at a multiplicity of infection (MOI) of 10 caused a log reduction in biofilm dens...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
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abstract::The oomycetes, a distinct phylogenetic lineage of fungus-like microorganisms, are heterokonts (stramenopiles) belonging to the supergroup Chromalveolata. Although the complete genomic sequences of a number of oomycetes have been reported, little information regarding the introns therein is available. Here, we investig...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-103
更新日期:2011-02-01 00:00:00
abstract::Vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. In this study, we demonstrate that V. cholerae O395 infection of intestinal epithelial cells results in the activation of Akt. Inhibition of Akt significantly decreases IL-1alpha, IL-6, and TNF-alpha production in V. cholerae i...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-093
更新日期:2009-11-01 00:00:00
abstract::In mycetomes of leafhoppers, Helochara communis, ultrastructural and histochemical studies revealed that spheroplast symbiotes (t-symbiotes) were converted to 'a-symbiotes' (so-called), with apparent loss of DNA--a phenomenon reminiscent of rhizobium-bacteroid conversion in certain legume nodules. Additional t-symbiot...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-028
更新日期:1975-02-01 00:00:00
abstract::The release of 14CO2 from 14C-labelled glucose(G-1(-14)C, G-3,4(-14)C, G-6(-14)C) was followed in phased cultures of Candida utilis grown in a glucose- mineral salts medium under altered conditions of carbon:nitrogen limitation at doubling times of 2,4 and 6, h. Changes in oxygen uptake and CO2 evolution were observe...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-150
更新日期:1975-07-01 00:00:00
abstract::The presence of any Listeria species in food may be an indicator of poor hygiene in food processing facilities. The biochemical identification of Listeria species is laborious and time consuming. Therefore, the development of novel identification methods that are rapid and simple to perform would be an asset. In this ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-054
更新日期:2010-08-01 00:00:00
abstract::Four modes of heating endotoxin in plasma and two different times of heating endotoxin in pyrogen-free water were compared. There were no significant differences in standard curves after heating endotoxin in plasma at 100 degrees C for 1 and for 10 min. However, the standard curve after heating for 10 min at 75 degree...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-140
更新日期:1986-10-01 00:00:00
abstract::Nodulation of Pisum sativum cv. Afghanistan by Rhizobium leguminosarum strain Tom can be blocked by the nonnodulating R. leguminosarum strain PF2. This system was used to study the dynamics of rhizobial binding to seedling root surfaces. Appreciable numbers of 35S-labeled rhizobia bound to the root surface within 2h a...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-099
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abstract::Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC st...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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abstract::Effects of temperature and anaerobic conditions on the replication of two bacteriophages, nt-1 and nt-6, of the estuarine bacterium Vibrio natriegens were studied. Reduction in temperature resulted in longer latent periods and reduced burst sizes for both phages. Replication under anaerobic conditions resulted in long...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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abstract::Auxotrophs in three different races of Pyricularia oryzae were obtained following ultraviolet mutagenesis, and tested for complementarity on minimal medium. Prototrophic growth resulted between combinations of different auxotrophs of single strains, but not between strains. The growth did not result form crossfeeding,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-079
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-197
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abstract::Caulobacters are biofilm-forming members of the natural flora of soil and aquatic environments, which exhibit several characteristics that make them attractive for development of high surface area microbial bioreactors or biosensors. Although caulobacters are well characterized genetically, little is known about their...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurosp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-147
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abstract::Bottles of medium were inoculated with both strains of Chromatiacea and Chlorobiaceae (mixed cultures) and immersed at differnt depths in the marine coastal waters of the Marseilles basin (France). After development, the in vivo absorption characteristics of the cells show the effect of the daylight penetration on the...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::A Gram-negative aerobic bacterium, designated as JN8, was isolated from activated sludge and soil in a pesticides factory in China. It was found that JN8 had a high capacity for degrading a broad range of type II pyrethroids and utilizing these pyrethroids as the sole carbon source for cell growth. The degradation rat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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abstract::Fluorescent Pseudomonas spp. isolated from fruiting bodies (FB) of Cantharellus cibarius were characterized physiologically and genetically and were compared with fluorescent Pseudomonas from forest soil and with sequences from the GenBank database. Pseudomonas spp. from FB differed physiologically from isolates from ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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abstract::The comparative ability of different methods to assess virulence of Listeria species was investigated in ten Listeria strains. All strains were initially subjected to pulsed-field gel electrophoresis analysis to determine their relatedness. Virulence characteristics were subsequently tested for by (i) determining the ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
abstract::This paper reports two different enterotoxigenic classes of the genus Aeromonas isolated from fishes. Strains of A. sobria were isolated from healthy fishes only wheras strains of A. hydrophila were isolated from both healthy and moribund fishes. Aeromonas sobria and hydrophilia strains produced a cytotoxic factor and...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-174
更新日期:1977-09-01 00:00:00