Abstract:
:We continued our study of high-molecular-mass proteases (HMMPs) using several strains of the genus Trichoderma, and other filamentous fungi (Botrytis cinerea, Aspergillus niger, Fusarium culmorum, and Penicillium purpurogenum). We found that five Trichoderma strains secreted HMMPs into the media after induction with bovine serum albumin. Botrytis cinerea and F. culmorum secreted proteases in the absence of inducer, while A. niger or P. purpurogenum did not secrete proteolytic activity (PA). The activity of HMMPs secreted by or intracellularly located in Trichoderma spp. represents the predominant part of cellular PA, according to zymogram patterns. This observation allowed the study of HMMPs' physiological role(s) independent from the secretion. In studying conidiation, we found that illumination significantly stimulated PA in Trichoderma strains. In the T. atroviride IMI 206040 strain, we demonstrated that this stimulation is dependent on the BLR1 and BLR2 receptors. No stimulation of PA was observed when mechanical injury was used as an elicitor of conidiation. Compounds used as inhibitors or activators of conidiation exerted no congruent effects on both PA and conidiation. These results do not favour a direct role of HMMPs in conidiation. Probably, HMMP activity may be involved in the process of the activation of metabolism during vegetative growth, differentiation, and aging-related processes.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Mat'at'a M,Galádová H,Varečka L,Šimkovič Mdoi
10.1139/cjm-2018-0670subject
Has Abstractpub_date
2019-09-01 00:00:00pages
653-667issue
9eissn
0008-4166issn
1480-3275journal_volume
65pub_type
杂志文章abstract::Of 33 streptococcal cultures belonging to serological group L, all bound human immunoglobulin (Ig) G, fibrinogen, and fibronectin; 32 bound bovine IgG; 31 bound alpha 2-macroglobulin; 5 bound albumin; and none bound either haptoglobin or IgA. The binding sites for IgG could be isolated from the L streptococci by tryps...
journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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更新日期:1980-05-01 00:00:00
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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doi:10.1139/m80-053
更新日期:1980-03-01 00:00:00
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-149
更新日期:1996-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-074
更新日期:2002-09-01 00:00:00
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
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doi:
更新日期:2001-07-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-004
更新日期:1981-01-01 00:00:00
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journal_title:Canadian journal of microbiology
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pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-190
更新日期:1981-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-05-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2016-08-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-062
更新日期:1981-04-01 00:00:00