Abstract:
:The effect of the transition metal compound trans-[Rh(4-ethylpyridine)4Cl2]Cl x 2H2O on the syntheses of DNA, RNA, and protein has been investigated for an auxotrophic bacterial strain, Escherichia coli JS-1, incapable of thymidine, uridine, and histidine syntheses. At low concentration (7.4 x 10(-6) M), this rhodium complex interferes with normal cell division and induces the formation of filaments comparable to those observed in the presence of the cis-(NH3)2PtClx antitumour agents. Once the suppressed growth rate of the filamenting cells has been taken into account, the rhodium compound is found not to alter macromolecular synthesis. Again this is consistent with similar observations made for the platinum compounds.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Seeman SL,Jack TRdoi
10.1139/m80-111subject
Has Abstractpub_date
1980-05-01 00:00:00pages
636-9issue
5eissn
0008-4166issn
1480-3275journal_volume
26pub_type
杂志文章abstract::The microbial community plays an important role in soil nutrient cycles and energy transformations in alpine grassland. In this study, we investigated the composition of the soil microbial community collected from alpine cold swamp meadow (ASM), alpine cold meadow (AM), and alpine cold desert steppe (ADS) within the B...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2017-0585
更新日期:2018-03-01 00:00:00
abstract::Rhizobia are used exclusively in agricultural systems for enhancing the ability of legumes to fix atmospheric nitrogen. Knowledge about the indigenous population is necessary for the selection and application of inoculant strains. In this study, we have assessed the genetic diversity of Bradyrhizobium strains isolated...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-139
更新日期:2001-02-01 00:00:00
abstract::Ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. All test isolates produced siderophores as indicated by a positive reaction in the FeCl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. Further, the test isolates were compar...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-012
更新日期:2006-06-01 00:00:00
abstract::Passive protective activities of three different classes of monoclonal antibodies in mice against challenge with strain ATCC 31432 (capsular type I) of Staphylococcus epidermidis were examined. Monoclonal IgM antibody passively protected mice against challenge with the homologous strain, whereas monoclonal IgG1 and Ig...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-065
更新日期:1991-05-01 00:00:00
abstract::The effects of selected culture conditions on production of Shiga-like toxin-II variant by an edema disease strain of Escherichia coli (412) and E. coli TB1 (pCG6) containing the cloned genes for Shiga-like toxin-II variant were examined. Incubation time, culture media, incubation temperature, starting pH of the cultu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-100
更新日期:1989-06-01 00:00:00
abstract::Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/m95-091
更新日期:1995-08-01 00:00:00
abstract::Dithiothreitol reacted, at pH 9.0, with the isolated cell walls of Methanospirillum hungatii, to release about 23% of the cell wall dry weight as a high molecular weight fraction (> 0.5 million daltons). Untreated walls consisted of 70% amino acids, 11% lipid, and 6.6% carbohydrate. Sugars were identified as rhamnose,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-017
更新日期:1980-02-01 00:00:00
abstract::In our study of opportunistic pathogens, we have some indication that Staphylococcus aureus can increase resistance in mice against Pseudomonas aeruginosa. Intraperitoneal injections of sublethal doses of S. aureus had a protective effect in mice against lethal doses of P. aeruginosa, more so if living and coagulase-p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1976-07-01 00:00:00
abstract::Cellobiose transport by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes was measured using randomly tritiated cellobiose. When assayed at the same concentration (1 mM), total cellobiose uptake was one-fourth to one-third that of total glucose uptake. The abilities of F. succinogenes to transpo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-021
更新日期:1991-02-01 00:00:00
abstract::This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-001
更新日期:1983-01-01 00:00:00
abstract::In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L. reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0007
更新日期:2015-06-01 00:00:00
abstract::Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-227
更新日期:1976-10-01 00:00:00
abstract::We report some characteristics of a ruminal bacterium (strain NPOH1) that metabolizes 3-nitropropanol, the toxic principle of various milk vetchs that are distributed worldwide. The gram-positive bacterium was nonmotile and did not produce spores. Growth of strain NPOH1 occurred under anaerobic conditions and was supp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-088
更新日期:1997-07-01 00:00:00
abstract::Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-021
更新日期:1989-01-01 00:00:00
abstract::Approximately 20% of Canada's oil supply now comes from the extraction of bitumen from the oil sands deposits in northeastern Alberta. The oil sands are strip-mined, and the bitumen is typically separated from sand and clays by an alkaline hot water extraction process. The rapidly expanding oil sands industry has mill...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-129
更新日期:2002-01-01 00:00:00
abstract::Nonlinear survivor curves were obtained when spores of Bacillus cereus were heated in physiological saline solution. Curvilinear survivor curves did not appear to be caused by experimental artifacts but by the heterogeneity of spore population with regard to heat resistance. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-217
更新日期:1975-10-01 00:00:00
abstract::The origin of the eukaryotic nucleus is difficult to reconstruct. While eukaryotic organelles (chloroplast, mitochondrion) are eubacterial endosymbionts, the source of nuclear genes has been obscured by multiple nucleotide substitutions. Using evolutionary parsimony, a newly developed rate-invariant treeing algorithm,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-017
更新日期:1989-01-01 00:00:00
abstract::The use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with approp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0261
更新日期:2020-01-01 00:00:00
abstract::Haemophilus paragallinarum secretes metalloproteases into different culture media lacking serum. Secreted proteins, concentrated by precipitation with 70% ammonium sulphate ((NH(4))(2)SO(4)) or methanol, displayed proteolytic activity at >100 kDa molecular mass in 10% polyacrylamide gels co-polymerized with porcine ge...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-067
更新日期:2005-10-01 00:00:00
abstract::Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-153
更新日期:1978-08-01 00:00:00
abstract::The effect of mechanical wounding or foliar diseases caused by Sclerotinia homoeocarpa or Rhizoctonia solani on the epiphytic yeast communities on creeping bentgrass and tall fescue were determined by leaf washing and dilution plating. Total yeast communities on healthy bentgrass and tall fescue leaves ranged from 7.9...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-073
更新日期:2004-10-01 00:00:00
abstract::Asexual sporulation in the fungus Aspergillus nidulans is controlled, in part, by a central regulatory pathway composed of the brlA, abaA, and wetA genes. The coding region of each of these genes was fused, in frame, to the threonine-inducible alcohol dehydrogenase promotor then stably incorporated into the A. nidulan...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-164
更新日期:1994-12-01 00:00:00
abstract::Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the results were interpreted ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W08-098
更新日期:2008-12-01 00:00:00
abstract::The present investigation was aimed to scrutinize the salt tolerance potential of plant-growth-promoting rhizobacteria (PGPR) isolated from rhizospheric soil of selected halophytes (Atriplex leucoclada, Haloxylon salicornicum, Lespedeza bicolor, Suaeda fruticosa, and Salicornica virginica) collected from high-saline f...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0668
更新日期:2015-04-01 00:00:00
abstract::Among nine eluents tested, 0.5% (w/v) isoelectric casein at pH 9.0 and 0.5% (w/v) non-fat dry milk (pH 9.0) were the most efficient in eluting poliovirus type 1 (Sabin) from Eustis fine sand. However, no significant difference was found between the overall (elution followed by concentration) virus recoveries by non-fa...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-130
更新日期:1979-08-01 00:00:00
abstract::Aspergillus caespitosus NRRL 5769 growing in broth containing small amounts of sitosterol produced substance(s) with greater inhibitory activity against Candida albicans ATCC 10231 than in broth without sitosterol. Subsequent isolation, purification, and structural elucidation yielded 5,6-dihydro-5(S)-acetoxy-6(S)-1,2...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-259
更新日期:1975-11-01 00:00:00
abstract::The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-033
更新日期:1992-03-01 00:00:00
abstract::A field site near Humboldt, Saskatchewan, was annually treated with hog or cattle manure and cropped to canola, spring wheat, barley, and canola from 1997 to 2000. During each growing season, soil was analyzed for microbial populations in terms of activity and community structure, and crops were assessed for root rot ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-069
更新日期:2003-09-01 00:00:00
abstract::A disseminated and fatal infection was established in C57BL mice, injected intraperitoneally with either Neisseria meningitidis B,2b or Haemophilus influenzae type b bacteria plus enhancement factors. The effects of mucin, hemoglobin, and iron dextran as enhancement of bacterial infectivity in mice were evaluated indi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-007
更新日期:1986-01-01 00:00:00
abstract::In Salmonella typhimurium LT2 the F factor of Escherichia coli K-12 replicates normally but is repressed; Flac+ cells give no visible lysis on solid media with male-specific phages, low frequency transfer of Flac+ (0.001-0.007 per donor cell), few f2 infective centers (0.002-0.006 per cell), and they propagate male-sp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-184
更新日期:1983-09-01 00:00:00