Production of Brucella melitensis Omp16 protein fused to the human interleukin 2 in Lactococcus lactis MG1363 toward developing a Lactococcus-based vaccine against brucellosis.

abstract：:An obligate methyltroph Methylomonas methylovora oxidized methylamine, formaldehyde, and formate. Enzymes oxidizing these substrates were detected in a cell-free system. Phenazine methosulfate-linked methylamine dehydrogenase was purified 21-fold. The enzyme had optimum activity at pH 7.5 and was stable at 60 degrees ...

journal_title：Canadian journal of microbiology

authors： Mehta RJ

更新日期：1977-04-01 00:00:00

abstract：:Considering the medical, biotechnological, and economical importance of actinobacteria, there is a continuous need to improve the tools for genetic engineering of a broad range of these microorganisms. Intergeneric conjugation has proven to be a valuable yet imperfect tool for this purpose. The natural resistance of m...

journal_title：Canadian journal of microbiology

authors： Allard N,Garneau D,Poulin-Laprade D,Burrus V,Brzezinski R,Roy S

更新日期：2015-08-01 00:00:00

abstract：:The catabolism of glucose by Streptomyces C5, a producer of anthracycline antibiotics, was investigated to determine the pathways that supply precursors for anthracycline biosynthesis. Carbons for the biosynthesis of epsilon-rhodomycinone, an anthracycline aglycone, from radiolabelled glucose were derived primarily fr...

journal_title：Canadian journal of microbiology

authors： Dekleva ML,Strohl WR

更新日期：1988-11-01 00:00:00

abstract：:In the frame of a survey of potentially endophytic N2-fixing Burkholderia associated with maize in Mexico, its country of origin, the soil of an indigenous maize field near Oaxaca was studied. Under laboratory conditions, plant seedlings of two ancient maize varieties were used as a trap to select endophyte candidates...

journal_title：Canadian journal of microbiology

authors： Estrada P,Mavingui P,Cournoyer B,Fontaine F,Balandreau J,Caballero-Mellado J

更新日期：2002-04-01 00:00:00

abstract：:The biochemical reactions, carbohydrate content, and 16S-rRNA sequences of Tatlockia (Legionella) maceachernii and Tatlockia micdadei strains were studied. Except for catalase activity, Tatlockia strains were relatively inert in the biochemical tests commonly used in clinical laboratories. Phenotypically, the two Tatl...

journal_title：Canadian journal of microbiology

authors： Fox KF,Brown A

更新日期：1993-05-01 00:00:00

abstract：:The deoxyribonucleic acid (DNA) of 36 isolates of Caryophanon latum and 3 isolates designated Caryophanon tenue, obtained from geographically diverse regions, has been examined for guanine plus cytosine (mol % G+C) base composition, genome size, and for relative reassociation levels. The isolates were found to be homo...

journal_title：Canadian journal of microbiology

authors： Adcock KA,Seidler RJ,Trentini WC

更新日期：1976-09-01 00:00:00

abstract：:Nonmotile and motility-altered mutants of Myxococcus xanthus have been obtained by the use of chemical mutagens, ultraviolet irradiation, and a procedure for selective spontaneous mutants. As judged by their behaviour on a variety of growth media, in both plate and slide culture, the mutants were divided into four gro...

journal_title：Canadian journal of microbiology

authors： MacRae TH,McCurdy HD

更新日期：1976-09-01 00:00:00

abstract：:Corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. Corynebacterium glutamicum his genes are located and transcribed in two unlinked loci, hisEG and hisDCB-orf1-orf2-hisHA-impA-hisFI. The latter his operon starts the transcription at the C residue localized...

journal_title：Canadian journal of microbiology

authors： Jung S,Chun JY,Yim SH,Lee SS,Cheon CI,Song E,Lee MS

更新日期：2010-02-01 00:00:00

abstract：:The effects of pressurized CO2 on the survival of Escherichia coli and the mechanism of cell inactivation were studied. Bacterial cultures were inoculated in nutrient broth and incubated at 30 degrees C for 18 h. Exposure of the cells to CO2 under pressures ranging from 2.5 to 25 MPa and at temperatures between 8 and ...

journal_title：Canadian journal of microbiology

authors： Oulé MK,Tano K,Bernier AM,Arul J

更新日期：2006-12-01 00:00:00

abstract：:Numbers of viable bacteria as indicated by colony-forming units (CFU), microbial adenosine triphosphate levels, dissolved organic carbon (DOC), particulate organic carbon and nitrogen (POC and PON), glucose concentration, heterotrophic potential (Vmax), and in situ heterotrophic activity (Vn) of near-surface microlaye...

journal_title：Canadian journal of microbiology

authors： Dietz AS,Albright LJ,Tuominen T

更新日期：1976-12-01 00:00:00

abstract：:A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurosp...

journal_title：Canadian journal of microbiology

authors： Chakraborty BN,Patterson NA,Kapoor M

更新日期：1991-11-01 00:00:00

abstract：:Seventeen of 22 group A meningococcal strains were separated into four serotypes by a microbactericidal assay using group A antisera. In addition, 6 of 19 of these strains were killed by a group B antiserum and similar cidal patterns were obtained with a group C antiserum. There was little correlation between type and...

journal_title：Canadian journal of microbiology

authors： Quan AL,Sippel JE

更新日期：1979-08-01 00:00:00

abstract：:Several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. This pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochro...

journal_title：Canadian journal of microbiology

authors： Jacobs NJ,O'Hara J,Gray CT

更新日期：1983-09-01 00:00:00

abstract：:Purification and chemical characterization of an immunosuppressive exopolysaccharide from Capnocytophaga ochracea strain 25 are described. This polysaccharide was extracted from spent culture medium by cold ethanol precipitation. Purification was accomplished by trichloroacetic acid and pronase treatments in combinati...

journal_title：Canadian journal of microbiology

authors： Dyer JK,Bolton RW

更新日期：1985-01-01 00:00:00

abstract：:Four modes of heating endotoxin in plasma and two different times of heating endotoxin in pyrogen-free water were compared. There were no significant differences in standard curves after heating endotoxin in plasma at 100 degrees C for 1 and for 10 min. However, the standard curve after heating for 10 min at 75 degree...

journal_title：Canadian journal of microbiology

authors： Piotrowicz BI,McCartney AC

更新日期：1986-10-01 00:00:00

abstract：:Bacterial diversity was assessed in water samples collected from several uranium mining wastes in Ger many and in the United States by using 16S rDNA and ribosomal intergenic spacer amplification retrievals. The results obtained using the 16S rDNA retrieval showed that the samples collected from the uranium mill taili...

journal_title：Canadian journal of microbiology

authors： Radeva G,Selenska-Pobell S

更新日期：2005-11-01 00:00:00

abstract：:During shadowing, a "cap" of metal develops on small particles. This cap increases apparent particle with (measured normal to the shadowing direction) by an extent which cannot be predetermined. The extent of this increase in particle size (here defined as the "cap," X) is estimated in the present method by using oppo...

journal_title：Canadian journal of microbiology

authors： Willison JH

更新日期：1985-07-01 00:00:00

abstract：:The morphology of Clostridium pasteurianum cells grown on 10(-2) M SO32- showed significant alteration in cell shape and the absence of the electron translucent reserve polysaccharide (amylopectin) when compared to sulfate-grown cells. At the lower sulfite concentrations (10(-3) and 10(-4)M SO32-) the cells showed the...

journal_title：Canadian journal of microbiology

authors： McCready RG,Costerton JW,Laishley EJ

更新日期：1976-02-01 00:00:00

abstract：:Administered orally, Quillaja saponin markedly potentiated the humoral immune responses of mice fed inactivated rabies vaccine, and significantly increased their resistance to subsequent intracerebral challenge with live rabies virus. Although mean serum neutralizing antibody titres were generally 8- to 16-fold higher...

journal_title：Canadian journal of microbiology

authors： Maharaj I,Froh KJ,Campbell JB

更新日期：1986-05-01 00:00:00

abstract：:Raw milk samples from bulk tanks in Ontario were analyzed for the presence of Listeria species. The overall incidence of Listeria species in raw milk was 12.4%. Listeria innocua was most frequently isolated and was found in 9.7% (43/445) of the raw milk samples, while L. monocytogenes and L. welshimeri were each found...

journal_title：Canadian journal of microbiology

authors： Farber JM,Sanders GW,Malcolm SA

更新日期：1988-02-01 00:00:00

abstract：:Eighteen yeast species of the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus, each one represented by its type strain, were investigated with the objective of evaluating their carotenoid composition. The pigments were extracted from yeast cells, quantified by high pressure liquid chromatography ...

journal_title：Canadian journal of microbiology

authors： Buzzini P,Innocenti M,Turchetti B,Libkind D,van Broock M,Mulinacci N

更新日期：2007-08-01 00:00:00

abstract：:Nodulation of soybeans by indigenous and inoculum strains of Bradyrhizobium japonicum was studied in field experiments in Wisconsin from 1983 to 86. Aqueous suspensions of bacteria were applied to seeds at the time of planting at levels of 7 × 10(7)-10(10) bacteria per 2.5-cm row. The predominant indigenous serogroup ...

journal_title：Canadian journal of microbiology

authors： McLoughlin TJ,Alt SG,Merlo PA

更新日期：1990-11-01 00:00:00

abstract：:In this work, we assayed the ability of newly prepared indolizine derivates (epimers) 6C and 6A to inhibit the growth of Mycobacterium smegmatis and used them for resistance induction. 6A inhibited the growth of M. smegmatis at a concentration of 100 μg/mL. No inhibitory effect was observed in the presence of 6C. By i...

journal_title：Canadian journal of microbiology

authors： Olejníková P,Kurucová M,Švorc L,Marchalín Š

更新日期：2013-02-01 00:00:00

abstract：:The sensitivity of delta red-gam delta ren mutants of bacteriophage lambda to Rex exclusion by lambda rexA+ rexB+ lysogens is modulated by the prophage cI repressor allele. We show the following: (i) lambda spi156 delta nin5 forms plaques on a cI+-rexA+-rexB+ lysogen with 10(5)-fold higher efficiency than on cI[Ts]-re...

journal_title：Canadian journal of microbiology

authors： Slavcev RA,Bull HJ,Hayes S

更新日期：2003-03-01 00:00:00

abstract：:Overexpression and purification are procedures used to allow functional and structural characterization of proteins. Many overexpressed proteins are partially or completely insoluble, and can not be easily purified. The NifA protein is an enhancer-binding protein involved in activating the expression of nif and some f...

journal_title：Canadian journal of microbiology

authors： Monteiro RA,Souza EM,Yates MG,Pedrosa FO,Chubatsu LS

更新日期：2000-11-01 00:00:00

abstract：:Seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (IA and IB), two on day 4 (IVA and IVB), one on day 8 (VIIIA), and two on day 9 (IXA and IXB). The lambs were reared in these isolators until ...

journal_title：Canadian journal of microbiology

authors： Fonty G,Gouet P,Nebout JM

更新日期：1989-03-01 00:00:00

abstract：:Experiments were carried out to elucidate linkage between methane consumption and mineralization of P from different phosphorous sources. The treatments were no CH4 no P amendment absolute control, with CH4 no P amendment control, with CH4 + inorganic P as Ca3(PO4)2 and with CH4 + organic P (sodium phytate). P sources...

journal_title：Canadian journal of microbiology

authors： Mohanty SR,Kumar A,Parmar R,Dubey G,Patra AK,Kollah B

更新日期：2020-12-10 00:00:00

abstract：:From electron-microscopical observations, a decreased metabolic activity in 3-day-old Candida albicans chlamydospores was suggested, and progressive deterioration in chlamydospores aged 2-8 months was shown. Oxygen utilization by chlamydospore-pseudomycelium (CSP) preparations was less than that by yeast, while 3-day-...

journal_title：Canadian journal of microbiology

authors： Miller SE,Finnerty WR

更新日期：1979-06-01 00:00:00

abstract：:Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed t...

journal_title：Canadian journal of microbiology

authors： Stoltz DB,Vinson SB,MacKinnon EA

更新日期：1976-07-01 00:00:00

abstract：:Dermatophytes are a group of fungi that are capable of invading keratinized tissues of humans and other animals. Antifungal susceptibility analysis and genetic studies by random amplification of polymorphic DNA (RAPD), have been used to detect polymorphism as well as determining the possible resistance of dermatophyte...

journal_title：Canadian journal of microbiology

authors： Brilhante RS,Cordeiro RA,Medrano DJ,Monteiro AJ,Sidrim JJ,Rocha MF

更新日期：2005-06-01 00:00:00