Abstract：
:From electron-microscopical observations, a decreased metabolic activity in 3-day-old Candida albicans chlamydospores was suggested, and progressive deterioration in chlamydospores aged 2-8 months was shown. Oxygen utilization by chlamydospore-pseudomycelium (CSP) preparations was less than that by yeast, while 3-day-old CSP preparations used significantly less O2 than 24-h CSP preparations. Amino acid incorporation was greater in yeast than in CSP preparations. Leucine incorporation by 20-h yeasts was twice that of 5-day yeasts and 5 times that of 20-h and 5-day CSP. Amino acid decarboxylation was similar in yeasts and CSP and was determined by end-product analyses to be via amino acid oxidase. Light microscopy autoradiography of [14C]leucine incorporation demonstrated that the metabolic activity in CSP preparations was due to the young growing tips of the pseudomycelium and not to mature chlamydospores. Yeasts did not take up trypan blue could be stained if first autoclaved or treated with 10% acid or 10% base. Young chlamydospores grown in the presence of trypan blue developed unstained and became permeable to the dye at 2 1/2 days. These data suggest that chlamydospores of C. albicans do not function in the classical role attributed to spores; i.e., mature chlamydospores cannot germinate, but rather age, deteriorate, and die.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Miller SE,Finnerty WRdoi
10.1139/m79-111subject
Has Abstractpub_date
1979-06-01 00:00:00pages
765-72issue
6eissn
0008-4166issn
1480-3275journal_volume
25pub_type
杂志文章abstract：:In Japan, antimicrobial agent therapy for patients with diarrhea due to enterovirulent organisms including enterohemorrhagic Escherichia coli (EHEC) is common, and norfloxacin (NFLX), fosfomycin, and kanamycin are recommended for EHEC treatment by the Japanese Ministry of Health and Welfare. The aim of this study was ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:Vaccination is the most promising strategy to reduce the incidence of pneumococcal infection. Although there are vaccines available, all of them are based on polysaccharide antigens (conjugated or not). In addition to their high cost, those vaccines do not cover all serotypes. To overcome these hindrances, we evaluate...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-083
更新日期：2012-09-01 00:00:00
abstract：:Methanol extracts were obtained from L. interrogans serovars icterohaemorrhagiae and canicola and L. biflexa serovar patoc. Human sera from 167 normal individuals and 40 patients with different infectious diseases tested by complement fixation tests showed negative reactions. Sera from 100 patients with a suspicion of...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-075
更新日期：1991-06-01 00:00:00
abstract：:Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-099
更新日期：1991-08-01 00:00:00
abstract：:Virulent strains of the fish pathogen Aeromonas salmonicida, which have surface S-layers (S+), efficiently adhere to, enter, and survive within macrophages. Here we report that S+ bacteria were 10- to 20-fold more adherent to non-phagocytic fish cell lines than S-layer-negative (S-) mutants. When reconstituted with ex...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-034
更新日期：2000-07-01 00:00:00
abstract：:The synergistic interactions between Pseudomonas aeruginosa and virulent or attenuated murine cytomegalovirus (MCMV) were compared in vivo. Virulent MCMV challenge at a dose of 5 X 10(5) pfu/mouse intraperitoneally, followed by intranasal superinfection with 5 X 10(6) cfu/mouse of Pseudomonas aeruginosa after 48 h res...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-161
更新日期：1987-10-01 00:00:00
abstract：:The influence of plant growth promoting (PGP) activity of bacterial communities recovered from each of six weed species (barnyard grass (Echinochloa crusfalli (L.) Beauv.), corn spurrey (Spergula arvensis L.), goldenrod (Sonchus sp.), Italian ryegrass (Lolium multiflorum L.), lamb's-quarters (Chenopodium album L.), an...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-110
更新日期：2001-11-01 00:00:00
abstract：:The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on beta-glucuronidase activity, using the fluorogenic substrat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-157
更新日期：1991-12-01 00:00:00
abstract：:Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-038
更新日期：2004-08-01 00:00:00
abstract：:The oomycetes, a distinct phylogenetic lineage of fungus-like microorganisms, are heterokonts (stramenopiles) belonging to the supergroup Chromalveolata. Although the complete genomic sequences of a number of oomycetes have been reported, little information regarding the introns therein is available. Here, we investig...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-103
更新日期：2011-02-01 00:00:00
abstract：:A disseminated and fatal infection was established in C57BL mice, injected intraperitoneally with either Neisseria meningitidis B,2b or Haemophilus influenzae type b bacteria plus enhancement factors. The effects of mucin, hemoglobin, and iron dextran as enhancement of bacterial infectivity in mice were evaluated indi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-007
更新日期：1986-01-01 00:00:00
abstract：:The in situ degradation of the two nitramine explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), was evaluated using a mixture of RDX and HMX, incubated anaerobically at 10 degrees C with marine sediment from a previous military dumping site of unexplod...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-112
更新日期：2004-02-01 00:00:00
abstract：:The aim of the present study was to explore a new approach based on the hemagglutination (HA) assay to understand the colonization ability of Shigella spp. To study colonization ability, an animal model of 4-day-old suckling mouse, was exploited. We characterized the HA activity of 48 Shigella strains, with erythroc...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-095
更新日期：2012-10-01 00:00:00
abstract：:To facilitate the recognition of plant viruses with similar characteristics a dendrogram of characterized viruses was constructed. The sequence of criteria included: type of nucleic acid; single or double stranded; presence or absence of lipid envelope; helical or nonhelical symmetry; and divided or single genome. Non...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-197
更新日期：1977-09-01 00:00:00
abstract：:Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-227
更新日期：1976-10-01 00:00:00
abstract：:Caldibacillus debilis strains GB1 and Tf display distinct phenotypes. Caldibacillus debilis GB1 is capable of anaerobic growth and can synthesize ethanol while C. debilis Tf cannot. Comparison of the GB1 and Tf genome sequences revealed that the genomes were highly similar in gene content and showed a high level of sy...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0464
更新日期：2019-06-01 00:00:00
abstract：:This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjec...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-083
更新日期：2007-10-01 00:00:00
abstract：:A Gram-negative aerobic bacterium, designated as JN8, was isolated from activated sludge and soil in a pesticides factory in China. It was found that JN8 had a high capacity for degrading a broad range of type II pyrethroids and utilizing these pyrethroids as the sole carbon source for cell growth. The degradation rat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0104
更新日期：2014-08-01 00:00:00
abstract：:Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-164
更新日期：1980-08-01 00:00:00
abstract：:The application of crossed immunoelectrophoresis to the analysis of a reference cytoplasm and cell envelope preparation from Pasteurella multocida serotype 1 revealed antigenic complexity not previously found. At least 55 cytoplasmic and 19 cell envelope antigens were clearly distinguished. Variation of anticytoplasm ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-118
更新日期：1980-06-01 00:00:00
abstract：:Staphylococcus aureus isolated from the cervix of an infertile woman was found to cause complete immobilization of human spermatozoa in vitro. Only the cell culture and cell-free supernatant showed immobilization activity, indicating that the sperm immobilization factor might be released extracellularly by the organis...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-032
更新日期：2009-07-01 00:00:00
abstract：:Pestalotiopsis species were most dominant endophytic species isolated from four medicinal plants including Terminalia arjuna, Terminalia chebula, Azadirachta indica, and Holarrhena antidysenterica. Thirty Pestalotiopsis species isolated from different parts of the medicinal plants were selected for the study. The anti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-070
更新日期：2008-09-01 00:00:00
abstract：:The aim of this study was to determine the effect of the rhizosphere of maize on the diversity of denitrifying bacteria. Community structure comparison was performed by constructing a collection of isolates recovered from bulk and maize planted soil. A total of 3240 nitrate-reducing isolates were obtained and 188 of t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-037
更新日期：2004-07-01 00:00:00
abstract：:Menadione reductase (EC 1.6.99.2) has been purified 67-fold from Clostridium tyrobutyricum extracts. The molecular weight was found to be 60 000 and the prosthetic group was identified as FMN on the basis of the enzymatic analysis data. The binding of FMN to the menadione dehydrogenase apoenzyme was relatively weak wi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-053
更新日期：1980-03-01 00:00:00
abstract：:Amoebae serve as environmental hosts to a variety of mycobacteria, including Mycobacterium avium and Mycobacterium marinum. Mycobacterium shottsii and Mycobacterium pseudoshottsii are waterborne species isolated from the spleens and dermal lesions of striped bass (Morone saxatilis) from the Chesapeake Bay. The optimal...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0079
更新日期：2013-08-01 00:00:00
abstract：:Five media and procedures were compared for their recovery of fecal streptococci on membrane filters. Growth and numbers of a variety of pure cultures were compared, and recoveries from raw sewage and sewage effluent were determined on the five media. A total of 512 isolates were identified and the specificity of each...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-219
更新日期：1980-11-01 00:00:00
abstract：:The sensitivity of delta red-gam delta ren mutants of bacteriophage lambda to Rex exclusion by lambda rexA+ rexB+ lysogens is modulated by the prophage cI repressor allele. We show the following: (i) lambda spi156 delta nin5 forms plaques on a cI+-rexA+-rexB+ lysogen with 10(5)-fold higher efficiency than on cI[Ts]-re...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-021
更新日期：2003-03-01 00:00:00
abstract：:Probiotics are usually isolated from the gastrointestinal tract of humans and animals. The search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. To our knowledge, no reports regarding the potential probiotic effect of bac...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0811
更新日期：2016-06-01 00:00:00
abstract：:Dietary exposure to heavy metals may have detrimental effects on human and animal health, even at low concentrations. Specific probiotic bacteria may have properties that enable them to bind toxins from food and water. We assessed the interaction of probiotic bacteria with cadmium and lead in vitro as an initial scree...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-043
更新日期：2006-09-01 00:00:00
abstract：:Overexpression and purification are procedures used to allow functional and structural characterization of proteins. Many overexpressed proteins are partially or completely insoluble, and can not be easily purified. The NifA protein is an enhancer-binding protein involved in activating the expression of nif and some f...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-094
更新日期：2000-11-01 00:00:00
