Abstract:
:The application of crossed immunoelectrophoresis to the analysis of a reference cytoplasm and cell envelope preparation from Pasteurella multocida serotype 1 revealed antigenic complexity not previously found. At least 55 cytoplasmic and 19 cell envelope antigens were clearly distinguished. Variation of anticytoplasm immunoglobulin concentration was a major determining factor in resolving the maximum array of cytoplasmic antigens. The use of intermediate gel modification of crossed immunoelectrophoresis permitted the recognition of antibodies in the preimmune rabbit serum against a number of cytoplasmic antigens and a single cell envelope antigen. This technique also demonstrated that reference cytoplasm obtained by 105,000 x g centrifugation of sonically disrupted pasteurellae and repeatedly washed reference cell envelope preparation contained antigens of either origin in amounts sufficient to elicit antibody responses in the host. Antisera to whole cells in the intermediate gel indicated that formalin killed P. multocida were capable of eliciting immune responses to both reference systems.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Bhasin JL,Lapointe-Shaw Ldoi
10.1139/m80-118subject
Has Abstractpub_date
1980-06-01 00:00:00pages
676-89issue
6eissn
0008-4166issn
1480-3275journal_volume
26pub_type
杂志文章abstract::The presence of any Listeria species in food may be an indicator of poor hygiene in food processing facilities. The biochemical identification of Listeria species is laborious and time consuming. Therefore, the development of novel identification methods that are rapid and simple to perform would be an asset. In this ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-054
更新日期:2010-08-01 00:00:00
abstract::Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-164
更新日期:1980-08-01 00:00:00
abstract::Sera from mice which have been vaccinated with BCG and challenged with old tuberculin contain gamma interferon. These same sera also express antibacterial activity. Using Staphylococcus aureus we demonstrated that its growth was inhibited at dilutions of sera as high as 1:320. A 4% concentration of sera reduced the gr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-084
更新日期:1986-05-01 00:00:00
abstract::Nonlinear survivor curves were obtained when spores of Bacillus cereus were heated in physiological saline solution. Curvilinear survivor curves did not appear to be caused by experimental artifacts but by the heterogeneity of spore population with regard to heat resistance. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-217
更新日期:1975-10-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-099
更新日期:1980-04-01 00:00:00
abstract::Ten bacteriophages infecting Micrococcus luteus have been characterized. All phages contain double-stranded DNA, of 64.3--73.5 mol% guanine plus cytosine (GC). The DNA of phage N7 has the highest GC content reported for any bacterial virus. No unusual bases have been found. The intracellular replicating DNAs of six ph...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1979-09-01 00:00:00
abstract::The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-239
更新日期:1976-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-136
更新日期:1988-06-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-236
更新日期:1978-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-090
更新日期:1990-07-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:1999-05-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Verticillium fungicola, isolated from Agaricus bisporus (commercial mushroom), produced significant extracellular hydrophobin when grown for 7 days in a static liquid culture of synthetic minimal medium. The hydrophobin was purified by precipitation with ammonium sulphate (80% saturation), Sephadex G-100 gel filtratio...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-098
更新日期:2002-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-158
更新日期:1977-08-01 00:00:00
abstract::A proline dehydrogenase which catalyzes the nicotinamide adenine dinucleotide (NAD) dependent oxidation of proline and the NADH-dependent reduction of delta 1-pyrroline 5-carboxylic acid (PCA) was purified from extracts of Clostridium sporogenes. Following purification, only one protein band was found on analytical po...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-147
更新日期:1981-09-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-212
更新日期:1983-10-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::Mucor alternans(van Tieghem) was found to be a diphasic species in that it grew exclusively in the yeast-like budding phase under anaerobic conditions in the complex medium yeast extract-peptone-glucose broth and in tissue-culture medium 199. In the latter medium this growth form occurred also at 37C, at an initial pH...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-010
更新日期:1975-01-01 00:00:00
abstract::Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base specific and phosphate chain length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-046
更新日期:1979-03-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-09-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::The course of intranasal infection of Sendai virus in CBA and DBA mice was investigated in animals decomplemented with purified cobra venom factor. The mice were decomplemented either immediately before inoculation or at 4 days postinfection. Depletion of complement after the infection had been established had no appa...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-072
更新日期:1982-05-01 00:00:00
abstract::Farnesol, a quorum sensing (QS) signal, is produced by Candida albicans during high density growth and has been found to inhibit morphogenesis. This QS auto-inducing signal was discovered to increase amino acid incorporation by C. albicans when concentrations of farnesol increased to 10 microg/mL in yeast nitrogen bro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2005-08-01 00:00:00
abstract::Spiroplasmas were cultured from nonsurface-sterilized flowers of magnolia tree (Magnolia grandiflora L.) and tulip tree (Liriodendron tulipfera L.) in Alameda, Solano, and Yolo counties in California. Spiroplasmas were also isolated from honey bees (Apis mellifera L.) collected in the vicinity of Davis and Berkeley, C...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-038
更新日期:1981-02-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-210
更新日期:1978-11-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:2013-05-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1987-09-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-218
更新日期:1977-10-01 00:00:00