Purification and partial characterization of proline dehydrogenase from Clostridium sporogenes.

abstract：:Biological nitrogen fixation (BNF) can be improved by optimizing the interaction between the rhizobial inoculant and pea (Pisum sativum L.), leading to increased productivity and reduced nitrogen (N) fertilizer use. Eight Rhizobium leguminosarum bv. viciae strains were used to inoculate the super-nodulating pea mutant...

journal_title：Canadian journal of microbiology

authors： Yang C,Bueckert R,Schoenau J,Diederichsen A,Zakeri H,Warkentin T

更新日期：2017-11-01 00:00:00

abstract：:The search for new antimicrobial compounds involves finding novel sources of chemotherapeutic compounds or manipulating and combining structures from existing molecules. Small antimicrobial peptides (AMPs) are components of innate immune defenses characterized in greatest detail in insect-derived AMPs. We have generat...

journal_title：Canadian journal of microbiology

authors： Plunkett RM,Murray SI,Lowenberger CA

更新日期：2009-05-01 00:00:00

abstract：:Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility w...

journal_title：Canadian journal of microbiology

authors： Laddaga RA,MacLeod RA

更新日期：1982-04-01 00:00:00

abstract：:The catalytic subunit of many Escherichia coli redox enzymes bares a twin-arginine translocation (Tat)-dependent signal peptide in its precursor, which directs the redox enzyme complex to this Sec-independent pathway. NarG of the E. coli nitrate reductase NarGHI complex possesses a vestige twin-arginine motif at its N...

journal_title：Canadian journal of microbiology

authors： Li H,Turner RJ

更新日期：2009-02-01 00:00:00

abstract：:The tuberculin activity of protoplasmic extracts isolated from living BCG was purified successively by gel filtration on Sephadex G-100 and G-75, and by electrophoresis on 7.5% and on gradient (6-18%) polyacrylamide gels. The tuberculin-active fractions, as determined in BCG-sensitized guinea pigs, were used as the st...

journal_title：Canadian journal of microbiology

authors： Laguerre M,Turcotte R

更新日期：1975-12-01 00:00:00

abstract：:Thirty Haemophilus strains and six Actinobacillus strains, all of porcine origin, were examined for their biochemical reactivity on API 20E and API ZYM test strips using dense cell suspensions (supplemented with NAD as appropriate) as strip inocula. When combined with a test for V-factor dependency, the use of both st...

journal_title：Canadian journal of microbiology

authors： O'Reilly T,Rosendal S,Niven DF

更新日期：1984-10-01 00:00:00

abstract：:5-Methoxymethyl-2'-deoxyuridine (MMUdR), a drug with potent antiviral activity in vitro against Herpes simplex virus, was investigated for its immunosuppressive effects. Doses as high as 2000mg/kg given daily for 9 days were not immunosupporessive as judged by the fact that treated animals produced normal immune respo...

journal_title：Canadian journal of microbiology

authors： Rouse BT,Babiuk LA,Gupta VS

更新日期：1977-08-01 00:00:00

abstract：:Considering the medical, biotechnological, and economical importance of actinobacteria, there is a continuous need to improve the tools for genetic engineering of a broad range of these microorganisms. Intergeneric conjugation has proven to be a valuable yet imperfect tool for this purpose. The natural resistance of m...

journal_title：Canadian journal of microbiology

authors： Allard N,Garneau D,Poulin-Laprade D,Burrus V,Brzezinski R,Roy S

更新日期：2015-08-01 00:00:00

abstract：:Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDREI) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenoty...

journal_title：Canadian journal of microbiology

authors： Parveen S,Farrah SR,Gonzalez-Bonilla C,Zamudio AV,Tamplin ML

更新日期：2003-01-01 00:00:00

abstract：:The gene-encoding HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus JCM2501 was amplified and expressed in Escherichia coli and the enzymatic features were examined. The enzyme was detected mainly in the membrane fraction. It catalyzed the sulfide-dependent menaquinone (MK) reduction showing special enz...

journal_title：Canadian journal of microbiology

authors： Shibata H,Suzuki K,Kobayashi S

更新日期：2007-09-01 00:00:00

abstract：:In this work, we assayed the ability of newly prepared indolizine derivates (epimers) 6C and 6A to inhibit the growth of Mycobacterium smegmatis and used them for resistance induction. 6A inhibited the growth of M. smegmatis at a concentration of 100 μg/mL. No inhibitory effect was observed in the presence of 6C. By i...

journal_title：Canadian journal of microbiology

authors： Olejníková P,Kurucová M,Švorc L,Marchalín Š

更新日期：2013-02-01 00:00:00

abstract：:The respiration of Candida lipolytica on n-tetradecane is decreased in the presence of bentonite. This inhibition is less pronounced by the introduction of hydrophobic chains on the bentonite. Oxygen demand of resting cells varies with the length of hydrophobic chains. In fermentor, addition of adsorbant does not stim...

journal_title：Canadian journal of microbiology

authors： Pareilleux A,Maignan C

更新日期：1976-08-01 00:00:00

abstract：:Fifty-two killer, factor-resistant, nuclear mutants were isolated from sensitive strains of yeast and assorted into three functional groups. All but one mutant owed their resistance to an alteration in the cell wall binding site for killer. In several mutant strains, an alteration at the site of killer binding was ass...

journal_title：Canadian journal of microbiology

authors： Al-Aidroos K,Bussey H

更新日期：1978-03-01 00:00:00

abstract：:Fungal chitinases play essential roles in chitin degradation, cell wall remodeling, chitin recycling, nutrition acquisition, autolysis, and virulence. In this study, 18 genes of the glycoside hydrolase 18 (GH18) family were identified in the Isaria cicadae genome. Seventeen of the genes belonged to chitinases and one ...

journal_title：Canadian journal of microbiology

authors： Peng Y,Wang L,Gao Y,Ye L,Xu H,Li S,Jiang J,Li G,Dang X

更新日期：2020-04-01 00:00:00

abstract：:The effects of the Clostridium difficile toxin were examined in HeLa and mouse adrenal tumor (MAT) cells. Cytotoxicity was evaluated by vital dye exclusion and 51Cr release. In both HeLa and MAT cells, C. difficile toxin caused rounding of virtually 100% of cells. This rounding was distinguishable from rounding produc...

journal_title：Canadian journal of microbiology

authors： Gurwith MJ,Langston C,Dunsmore B

更新日期：1982-01-01 00:00:00

abstract：:Solid media were used to determine which extracellular hydrolytic enzymes are produced by Phytophthora parasitica; P. parasitica var. nicotianae, races 0, 1, and 3; and several other Phytophthora spp. Most isolates produced RNase, DNase, phosphatase, lipase, and cellulase uniformly. All race 3 isolates of P. parasitic...

journal_title：Canadian journal of microbiology

authors： McIntyre JL,Hankin L

更新日期：1978-01-01 00:00:00

abstract：:Polymerase chain reaction amplification was used to compare different regions of the cytochrome c3 gene from nine strains of Desulfovibrio vulgaris, to examine homology within the species. Six 30-base polymerase chain reaction primers and three probes were synthesized on the basis of the published nucleic acid sequenc...

journal_title：Canadian journal of microbiology

authors： Kwoh DY,Vedvick TS,McCue AF,Gevertz D

更新日期：1993-04-01 00:00:00

abstract：:The effect of the transition metal compound trans-[Rh(4-ethylpyridine)4Cl2]Cl x 2H2O on the syntheses of DNA, RNA, and protein has been investigated for an auxotrophic bacterial strain, Escherichia coli JS-1, incapable of thymidine, uridine, and histidine syntheses. At low concentration (7.4 x 10(-6) M), this rhodium ...

journal_title：Canadian journal of microbiology

authors： Seeman SL,Jack TR

更新日期：1980-05-01 00:00:00

abstract：:The presence and taxonomy of endophytic bacteria of the entire aerial parts of crocus (Crocus albiflorus), a wildflower native in the Alps, were investigated. A combination of plating of plant macerates, isolation and sequence identification of isolates, and direct 16S rDNA PCR amplification followed by whole-communit...

journal_title：Canadian journal of microbiology

authors： Reiter B,Sessitsch A

更新日期：2006-02-01 00:00:00

abstract：:Neisseria polysaccharea (LNP 462, NCTC 11858), proposed as a prototype strain constituting a new taxon in the genus Neisseria, produces copious amounts of polysaccharide when grown on agar containing 1-5% sucrose. Plate-grown cells produced an exocellular polysaccharide which was composed of D-glucose, had [alpha]D +2...

journal_title：Canadian journal of microbiology

authors： Riou JY,Guibourdenche M,Perry MB,MacLean LL,Griffith DW

更新日期：1986-12-01 00:00:00

abstract：:Rabbit antisera prepared to meningococcal serogroups Y and W135 strains were compared with horse antisera using the antiserum agar method (ASA) for the serogroup identification of Neisseria meningitidis. Thirty-seven group Y strains formed immunoprecipitates with the Y rabbit serum only, whereas the same Y strains for...

journal_title：Canadian journal of microbiology

authors： Ashton FE,Ryan A,Diena BB

更新日期：1980-05-01 00:00:00

abstract：:A strain of Pseudomonas putida grew rapidly on cyclohexanecarboxylic acid as a sole source of carbon. A CoA-mediated beta-oxidation pathway was induced for the metabolism of the compound. The organism could not utilize 3-cyclohexenecarboxylic acid as a sole source of carbon for growth, but cells grown on gluconate in ...

journal_title：Canadian journal of microbiology

authors： Blakley ER,Papish B

更新日期：1982-12-01 00:00:00

abstract：:In our study of opportunistic pathogens, we have some indication that Staphylococcus aureus can increase resistance in mice against Pseudomonas aeruginosa. Intraperitoneal injections of sublethal doses of S. aureus had a protective effect in mice against lethal doses of P. aeruginosa, more so if living and coagulase-p...

journal_title：Canadian journal of microbiology

authors： deRepentigny J,Mathieu LG,Coallier-Ascah J

更新日期：1976-07-01 00:00:00

abstract：:The purpose of this study was to examine expression and regulation of 6 multidrug efflux systems, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY, MexJK, and MexVW, in 13 non-cystic fibrosis (CF) clinical isolates of Pseudomonas aeruginosa. These isolates displayed a high level of resistance to many clinically imp...

journal_title：Canadian journal of microbiology

authors： Poonsuk K,Tribuddharat C,Chuanchuen R

更新日期：2014-07-01 00:00:00

abstract：:The open reading frame pp0053, which has a high homology with the sequence of mitochondrial sulfide dehydrogenase (HMT2) conferring cadmium tolerance in fission yeast, was amplified from Pseudomonas putida KT2440 and expressed in Escherichia coli JM109(DE3). The isolated and purified PP0053-His showed absorption spect...

journal_title：Canadian journal of microbiology

authors： Shibata H,Kobayashi S

更新日期：2006-08-01 00:00:00

abstract：:Cells of five Bacteroides species were examined following treatment with homologous antisera and staining with ruthenium red. They were enveloped by glycocalyces and these extensive fibrous exopolysaccharide matrices were fully retained as an integral "capsule" by some cells, while other cells showed "capsule" as well...

journal_title：Canadian journal of microbiology

authors： Lambe DW Jr,Mayberry-Carson KJ,Ferguson KP

更新日期：1984-06-01 00:00:00

abstract：:Amoebae serve as environmental hosts to a variety of mycobacteria, including Mycobacterium avium and Mycobacterium marinum. Mycobacterium shottsii and Mycobacterium pseudoshottsii are waterborne species isolated from the spleens and dermal lesions of striped bass (Morone saxatilis) from the Chesapeake Bay. The optimal...

journal_title：Canadian journal of microbiology

authors： Gupta T,Fine-Coulson K,Karls R,Gauthier D,Quinn F

更新日期：2013-08-01 00:00:00

abstract：:Characterization and enumeration of the adherent epimural community of the rumen wall of young, conventionally reared lambs were carried out from 2 to 21 days after birth. Three hundred strains were isolated by anaerobic procedures from three sites: dorsal, ventral, and caudal sacs, and from the sloughed epithelial ce...

journal_title：Canadian journal of microbiology

authors： Rieu F,Fonty G,Gouet P

更新日期：1989-07-01 00:00:00

abstract：:Urease activity was measured using whole cells of both long (swarming) and short (nonswarming) populations of Proteus mirabilis from casein hydrolysate agar (CHA) and broth (CHB) cultures, and from brain heart infusion broth (BHIB) cultures. Urease is a constitutive enzyme for both long and short cells, but its activi...

journal_title：Canadian journal of microbiology

authors： Jin T,Murray RG

更新日期：1987-04-01 00:00:00

abstract：:Soil bacteria that utilize inositol hexaphosphate (IHP) were isolated from a range of soils using defined selection media. An analysis of 200 randomly selected isolates indicated that less than 0.5% of the culturable population of soil bacteria were capable of using IHP as a sole source of C and P. From a further 238 ...

journal_title：Canadian journal of microbiology

authors： Richardson AE,Hadobas PA

更新日期：1997-06-01 00:00:00