Abstract:
:Rabbit antisera prepared to meningococcal serogroups Y and W135 strains were compared with horse antisera using the antiserum agar method (ASA) for the serogroup identification of Neisseria meningitidis. Thirty-seven group Y strains formed immunoprecipitates with the Y rabbit serum only, whereas the same Y strains formed immunoprecipitates with both the Y and W135 horse antisera. Forty-seven W135 strains formed specific immunoprecipitates with both the rabbit and horse W135 antisera by ASA. None of the 166 meningococcal isolates, representative of other meningococcal serogroups, formed immunoprecipitates with the groups Y and W135 rabbit or horse antisera. Use of specific Y and W135 rabbit antisera in ASA provides an improved technique for the serogroup differentiation of groups Y and W135 meningococci.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Ashton FE,Ryan A,Diena BBdoi
10.1139/m80-109subject
Has Abstractpub_date
1980-05-01 00:00:00pages
630-2issue
5eissn
0008-4166issn
1480-3275journal_volume
26pub_type
杂志文章abstract::We continued our study of high-molecular-mass proteases (HMMPs) using several strains of the genus Trichoderma, and other filamentous fungi (Botrytis cinerea, Aspergillus niger, Fusarium culmorum, and Penicillium purpurogenum). We found that five Trichoderma strains secreted HMMPs into the media after induction with b...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0670
更新日期:2019-09-01 00:00:00
abstract::Pseudomonas maltophilia has been identified in the rhizosphere of several cultured plants: cabbage, rape, mustard, corn, beet. In comparison with nonrhizospheric soil, all the rhizospheric samples analysed contain more of this Pseudomonas and the most important stimulation is supplied by cruciferous plants. The possib...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::Products obtained from the action of arylamine synthetase on [G-14C]chorismic acid were fractionated by gel filtration and ion exchange column chromatography to yield a partially purified radioactive component with an arylamine function. From its ultraviolet absorption spectrum and thin-layer chromatographic behaviour...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-032
更新日期:1976-02-01 00:00:00
abstract::Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-173
更新日期:1987-11-01 00:00:00
abstract::The purpose of this study was to examine expression and regulation of 6 multidrug efflux systems, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY, MexJK, and MexVW, in 13 non-cystic fibrosis (CF) clinical isolates of Pseudomonas aeruginosa. These isolates displayed a high level of resistance to many clinically imp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0239
更新日期:2014-07-01 00:00:00
abstract::A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-151
更新日期:1977-08-01 00:00:00
abstract::Ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. All test isolates produced siderophores as indicated by a positive reaction in the FeCl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. Further, the test isolates were compar...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-012
更新日期:2006-06-01 00:00:00
abstract::Several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. This pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-177
更新日期:1983-09-01 00:00:00
abstract::Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base specific and phosphate chain length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-046
更新日期:1979-03-01 00:00:00
abstract::Effects of Trichoderma harzianum and T. polysporum on chlamydospore production by two isolates of Phytophthora cinamomi were determined over a 21-day period in nonsterile, sterilized, and sterilized amended soil. Trichoderma was either coinoculated with P. cinnamomi or added to the cultures on day 3 of the incubation ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-042
更新日期:1977-03-01 00:00:00
abstract::In this work, we disrupted one of three putative phosphatidylinositol phospholipase C genes of Aspergillus nidulans and studied its effect on carbon source sensing linked to vegetative mitotic nuclear division. We showed that glucose does not affect nuclear division rates during early vegetative conidial germination (...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-034
更新日期:2011-07-01 00:00:00
abstract::Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Mutations in the DNA polymerase locus of phage, bacteria, and eukaryotic may change the mutation rates at other loci of the genome. We used resistance to phosphonoacetate to select mutants of herpes simplex virus with mutated DNA polymerase and then determined the reversion frequency of viral thymidine kinase mutation...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-084
更新日期:1984-05-01 00:00:00
abstract::Histological examination of oesophagus and stomach specimens from 28 guinea pigs (Cavia porcellus) revealed microbes associated with the epithelial surface of these organs. Layers of Gram-positive cocci were associated with oesophageal tissue in all the animals. Yeasts (Torulop-sid pintolopesii) were associated with s...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-182
更新日期:1978-09-01 00:00:00
abstract::Dermatophytes are a group of fungi that are capable of invading keratinized tissues of humans and other animals. Antifungal susceptibility analysis and genetic studies by random amplification of polymorphic DNA (RAPD), have been used to detect polymorphism as well as determining the possible resistance of dermatophyte...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-024
更新日期:2005-06-01 00:00:00
abstract::An obligate methyltroph Methylomonas methylovora oxidized methylamine, formaldehyde, and formate. Enzymes oxidizing these substrates were detected in a cell-free system. Phenazine methosulfate-linked methylamine dehydrogenase was purified 21-fold. The enzyme had optimum activity at pH 7.5 and was stable at 60 degrees ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-059
更新日期:1977-04-01 00:00:00
abstract::The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0770
更新日期:2016-07-01 00:00:00
abstract::It is important to understand the dynamics of tetracycline-resistant bacteria (TRB) and tetracycline resistance genes (TRGs) in bulk and rhizosphere soils for evaluating the spread of TRGs from pig manure to human. In this work, a greenhouse experiment was conducted to investigate the difference in abundance of TRB, t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2016-0767
更新日期:2017-07-01 00:00:00
abstract::Antibiotic-resistant and antimetabolite-resistant mutants of the nitrogen-fixing symbiotic bacterium Frankia were isolated to provide strains with genetic backgrounds amenable to genetic analysis. The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV light on four Frankia strains were investigated. U...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-013
更新日期:2004-04-01 00:00:00
abstract::DNA hybridization studies were conducted to determine the taxonomic status of the aquatic group of Metschnikowia species and their varieties. Among the DNAs of the four varieties of Metschnikowia bicuspidata, that of Metschnikowia bicuspidata var. australis showed 37 to 51% relative binding with the DNAs of Metschniko...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-170
更新日期:1985-10-01 00:00:00
abstract::Humicola lutea 103 is a copper-tolerant fungal strain able to grow in the presence of 300 μg·mL(-1) Cu(2+) under submerged cultivation. To prevent the consequences of copper overload, microorganisms have evolved molecular mechanisms that regulate its uptake, intracellular traffic, storage, and efflux. In spite of this...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-112
更新日期:2012-12-01 00:00:00
abstract::The presence of any Listeria species in food may be an indicator of poor hygiene in food processing facilities. The biochemical identification of Listeria species is laborious and time consuming. Therefore, the development of novel identification methods that are rapid and simple to perform would be an asset. In this ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-054
更新日期:2010-08-01 00:00:00
abstract::The effects of inhibition of protein synthesis on the cell size distributions of rel+ and relA- derivatives of Escherichia coli K-12 were determined. Amino acid deprivation resulted in a reduction in the cell sizes of rel+ strains but not of relA- strains. Treatment with chloramphenicol (CAM) did not alter the size di...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-127
更新日期:1978-06-01 00:00:00
abstract::Subtyping Neisseria meningitidis by methods that rely on monoclonal antibody (mAb) reactivity results in an unusually high number of strains that are not subtypeable. To subtype 48 strains isolated (1993-1994) in the province of Quebec that were not subtypeable by mAb-based techniques, we used DNA sequencing of the va...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-070
更新日期:1996-05-01 00:00:00
abstract::Auxotrophs in three different races of Pyricularia oryzae were obtained following ultraviolet mutagenesis, and tested for complementarity on minimal medium. Prototrophic growth resulted between combinations of different auxotrophs of single strains, but not between strains. The growth did not result form crossfeeding,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-079
更新日期:1976-04-01 00:00:00
abstract::The deliberate application of the methodology of contemporary molecular genetics to problems in bacterial systematics has led to a broad new understanding of the evolutionary history of both prokaryotes and eukaryotes. In this review, I discuss some of the major conclusions of this endeavour and try to predict future ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/m88-093
更新日期:1988-04-01 00:00:00
abstract::The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on beta-glucuronidase activity, using the fluorogenic substrat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-157
更新日期:1991-12-01 00:00:00
abstract::The IncHII plasmid pHH1508a (208 kilobases) encodes resistance to potassium tellurite, trimethoprim, and streptomycin. Conjugative pili encoded by pHH1508a were isolated, purified, and used for preparation of anti-H pilus antiserum. Immuno-gold labelling experiments using H pilus specific antiserum showed that antigen...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-043
更新日期:1989-02-01 00:00:00
abstract::During shadowing, a "cap" of metal develops on small particles. This cap increases apparent particle with (measured normal to the shadowing direction) by an extent which cannot be predetermined. The extent of this increase in particle size (here defined as the "cap," X) is estimated in the present method by using oppo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-114
更新日期:1985-07-01 00:00:00