Abstract：
:Thirty Haemophilus strains and six Actinobacillus strains, all of porcine origin, were examined for their biochemical reactivity on API 20E and API ZYM test strips using dense cell suspensions (supplemented with NAD as appropriate) as strip inocula. When combined with a test for V-factor dependency, the use of both strips allowed adequate differentiation of closely related organisms. Numerical taxonomic analysis of the data demonstrated that the majority of the haemophili and actinobacilli studied could be placed in one of four major clusters; these clusters contained, respectively, the H. pleuropneumoniae--A. pleuropneumoniae strains, the H. parasuis strains, strains belonging to Haemophilus taxon "minor group," and strains belonging to an unusual group of mannitol-positive, urease-negative haemophili. A representative of Haemophilus species taxon C and an unusual Actinobacillus isolate appeared to be comparatively unrelated to organisms in the four major clusters. Although it may, on occasion, be difficult to place an unusual isolate in any one particular group, owing to the uncertain taxonomy of some of these organisms, it is concluded that API test strips can serve as useful tools for the characterization and differentiation of porcine haemophili and actinobacilli.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
O'Reilly T,Rosendal S,Niven DFdoi
10.1139/m84-195subject
Has Abstractpub_date
1984-10-01 00:00:00pages
1229-38issue
10eissn
0008-4166issn
1480-3275journal_volume
30pub_type
杂志文章abstract：:The presence and taxonomy of endophytic bacteria of the entire aerial parts of crocus (Crocus albiflorus), a wildflower native in the Alps, were investigated. A combination of plating of plant macerates, isolation and sequence identification of isolates, and direct 16S rDNA PCR amplification followed by whole-communit...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-109
更新日期：2006-02-01 00:00:00
abstract：:Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obta...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-107
更新日期：1988-05-01 00:00:00
abstract：:The metal-binding properties of Pseudomonas aeruginosa PAO1 biofilms were investigated using four metals (Cu, Fe, Au, and La). All but one of the metals (i.e., Cu) were bound by the biofilms in amounts that were significantly greater than those bound by planktonically grown cells of the same strain. Lanthanum precipit...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:The goal of this study was to assess the presence and surfactant potential of naturally occurring microbes from a tropical soil with petrochemical contamination. Microorganisms in a soil sample from a Brazilian landfarm were isolated and grown on petroleum as the sole carbon source. Of 60 isolates screened for petrole...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-052
更新日期：2007-08-01 00:00:00
abstract：:Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-015
更新日期：1989-01-01 00:00:00
abstract：:The PII protein is apparently involved in the control of NifA activity in Herbaspirillum seropedicae. To evaluate the probable role of PII in signal transduction, uridylylation assays were conducted with purified H. seropedicae PII and Escherichia coli GlnD, or a cell-free extract of H. seropedicae as sources of uridy...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-018
更新日期：2001-04-01 00:00:00
abstract：:Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-148
更新日期：1976-07-01 00:00:00
abstract：:Antibiotic-resistant and antimetabolite-resistant mutants of the nitrogen-fixing symbiotic bacterium Frankia were isolated to provide strains with genetic backgrounds amenable to genetic analysis. The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV light on four Frankia strains were investigated. U...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-013
更新日期：2004-04-01 00:00:00
abstract：:The 40-kDa porin protein of Haemophilus influenzae type b was reconstituted into proteoliposomes. The relative rates of diffusion of small uncharged sugars across the channels formed by this protein were determined by measuring the rates of osmotic swelling of the liposomes. From these rates, a pore diameter of 1.8 nm...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-027
更新日期：1988-02-01 00:00:00
abstract：:The application of crossed immunoelectrophoresis to the analysis of a reference cytoplasm and cell envelope preparation from Pasteurella multocida serotype 1 revealed antigenic complexity not previously found. At least 55 cytoplasmic and 19 cell envelope antigens were clearly distinguished. Variation of anticytoplasm ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-118
更新日期：1980-06-01 00:00:00
abstract：:Melanin biosynthesis in Verticillium dahliae Kleb, was studied with mutants deficient for normal black melanin or for production of microsclerotia. Seven genetically different mutants had apparent blocks in melanin biosynthesis. Four mutants (brm-1 to -4) produced brown microsclerotia and extruded pigments into media;...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-115
更新日期：1976-06-01 00:00:00
abstract：:Aspergillus caespitosus NRRL 5769 growing in broth containing small amounts of sitosterol produced substance(s) with greater inhibitory activity against Candida albicans ATCC 10231 than in broth without sitosterol. Subsequent isolation, purification, and structural elucidation yielded 5,6-dihydro-5(S)-acetoxy-6(S)-1,2...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-259
更新日期：1975-11-01 00:00:00
abstract：:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent micr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-063
更新日期：1979-03-01 00:00:00
abstract：:Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w98-225
更新日期：1999-02-01 00:00:00
abstract：:One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20-18, a rifa...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-029
更新日期：2001-05-01 00:00:00
abstract：:The mouse model of intraperitoneal sepsis with Proteus rettgeri was used to evaluate the anti-endotoxic effect of polymyxin B sulfate. An unexpected reversal of the usual protective effect of polymyxin in experimental enterobacterial sepsis was observed in which the lethality of the infection was enhanced. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-039
更新日期：1980-02-01 00:00:00
abstract：:Numbers of viable bacteria as indicated by colony-forming units (CFU), microbial adenosine triphosphate levels, dissolved organic carbon (DOC), particulate organic carbon and nitrogen (POC and PON), glucose concentration, heterotrophic potential (Vmax), and in situ heterotrophic activity (Vn) of near-surface microlaye...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-251
更新日期：1976-12-01 00:00:00
abstract：:Menadione reductase (EC 1.6.99.2) has been purified 67-fold from Clostridium tyrobutyricum extracts. The molecular weight was found to be 60 000 and the prosthetic group was identified as FMN on the basis of the enzymatic analysis data. The binding of FMN to the menadione dehydrogenase apoenzyme was relatively weak wi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-053
更新日期：1980-03-01 00:00:00
abstract：:Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDREI) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenoty...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-004
更新日期：2003-01-01 00:00:00
abstract：:Three types of bacteria are associated with leprosy: Mycobacterium leprae, leprosy-derived corynebacteria (LDC), and armadillo-derived mycobacteria (ADM). The immunological relationships between these three types of bacteria and Mycobacterium bovis BCG, used as a reference, were determined by cross-immunoelectrophores...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-083
更新日期：1994-06-01 00:00:00
abstract：:The fine structure of conidia and immature resting spores of the entomogenous fungus Entomophthora apiculata is described. The two cell types contain comparable organelles and quantities of lipid, and possess a similar cell wall structure. The large quantity of stored lipid and numerous mitochondria in each cell type ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-067
更新日期：1977-04-01 00:00:00
abstract：:The IncHII plasmid pHH1508a (208 kilobases) encodes resistance to potassium tellurite, trimethoprim, and streptomycin. Conjugative pili encoded by pHH1508a were isolated, purified, and used for preparation of anti-H pilus antiserum. Immuno-gold labelling experiments using H pilus specific antiserum showed that antigen...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-043
更新日期：1989-02-01 00:00:00
abstract：:The diversity of 16 strains of chickpea-infective rhizobia from various geographical origins was analysed using genotypic and phenotypic approaches. Multilocus enzyme electrophoresis was performed, and restriction fragment length polymorphisms of the amplified 16S + IGS (intergenic spacer) rRNA gene, assimilation of 1...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-057
更新日期：1994-05-01 00:00:00
abstract：:The effects of inhibition of protein synthesis on the cell size distributions of rel+ and relA- derivatives of Escherichia coli K-12 were determined. Amino acid deprivation resulted in a reduction in the cell sizes of rel+ strains but not of relA- strains. Treatment with chloramphenicol (CAM) did not alter the size di...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-127
更新日期：1978-06-01 00:00:00
abstract：:Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-153
更新日期：1978-08-01 00:00:00
abstract：:The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-239
更新日期：1976-11-01 00:00:00
abstract：:Strict anaerobic cultures of Mucor hiemalis lead to major modifications of the quantitative and qualitative composition of sterols. Sterols of which the lateral chain contains an ethyl or ethylidene group in C24 are for the first time brought to evidence in a Mucorales. Isolation in the same conditions of E-ethylidene...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-095
更新日期：1983-05-01 00:00:00
abstract：:Most prokaryotic chromosomes contain a number of toxin-antitoxin (TA) modules consisting of a pair of genes that encode 2 components, a stable toxin and its cognate labile antitoxin. TA systems are also known as addiction modules, since the cells become "addicted" to the short-lived antitoxin product (the unstable ant...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/w2012-025
更新日期：2012-05-01 00:00:00
abstract：:Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-063
更新日期：1982-04-01 00:00:00
abstract：:Cell-free extracts prepared from the extremely halophilic bacterium Halobacterium saccharovorum oxidize galactose and accumulate a product which reacts as if it were a lactone. The product does not act as a reducing sugar and contains all six of the carbon atoms initially present in galactose. The product was jugged t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-174
更新日期：1976-08-01 00:00:00
