Abstract:
:The metal-binding properties of Pseudomonas aeruginosa PAO1 biofilms were investigated using four metals (Cu, Fe, Au, and La). All but one of the metals (i.e., Cu) were bound by the biofilms in amounts that were significantly greater than those bound by planktonically grown cells of the same strain. Lanthanum precipitation appeared to be limited to the base of the biofilms and was not promoted by a shift in lipopolysaccharide production by the cells.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Langley S,Beveridge TJsubject
Has Abstractpub_date
1999-07-01 00:00:00pages
616-22issue
7eissn
0008-4166issn
1480-3275journal_volume
45pub_type
杂志文章abstract::Characterization and enumeration of the adherent epimural community of the rumen wall of young, conventionally reared lambs were carried out from 2 to 21 days after birth. Three hundred strains were isolated by anaerobic procedures from three sites: dorsal, ventral, and caudal sacs, and from the sloughed epithelial ce...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-114
更新日期:1989-07-01 00:00:00
abstract::A comparative study of the pathogenicity of Candida albicans morphological mutant strains was made to relate chlamydospore production, germ tube formation, and proteolytic activity to candidiasis in mice. It was observed that the mycelium strains were more lethal than the yeast-like strains and that neither chlamydosp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-093
更新日期:1975-05-01 00:00:00
abstract::Campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. No adhesins specific for C. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. Scanning electron microscopy revealed C. fetus attachment to the serosal surface...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w07-115
更新日期:2008-01-01 00:00:00
abstract::Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and th...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-236
更新日期:1978-12-01 00:00:00
abstract::Biological nitrogen fixation (BNF) can be improved by optimizing the interaction between the rhizobial inoculant and pea (Pisum sativum L.), leading to increased productivity and reduced nitrogen (N) fertilizer use. Eight Rhizobium leguminosarum bv. viciae strains were used to inoculate the super-nodulating pea mutant...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2017-0281
更新日期:2017-11-01 00:00:00
abstract::Staphylococcus aureus was grown in a rich peptone medium which became alkaline with continued incubation. Cells were grown at 37 degrees C and in the same medium containing 1 M NaCl at 46 degrees C, a temperature at which this organism can grow only when protected by NaCl. Cells of these cultures are hereafter called ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-049
更新日期:1983-03-01 00:00:00
abstract::In this study, 16S rRNA gene primers were designed to complement the suite of already available PCR primers for the detection of different methanogens involved in biogas production through anaerobic digestion by SYBR Green real-time PCR. Primers designed for use in TaqMan real-time PCR for the organisms Methanosaeta, ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-157
更新日期:2009-05-01 00:00:00
abstract::Aluminum (Al) toxicity is a major problem affecting soil fertility, microbial diversity, and nutrient uptake of plants. Rhizobia response and legume interaction under Al conditions are still unknown; it is important to understand how to develop and improve legume cultivation under Al stress. In this study, rhizobia re...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0019
更新日期:2018-08-01 00:00:00
abstract::Investigations of microbial diversity in Antarctic are important to begin to understand ecosystem functioning and decomposition processes. This study documents fungi at 9 historic sites on the Antarctic Peninsula collected from wooden structures, other organic materials, and soils during a joint National Science Found...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W08-120
更新日期:2009-01-01 00:00:00
abstract::Glutaraldehyde, the biological fixative of choice in the cytochemical localization of the phosphatases, was investigated for its effects on Pseudomonas aeruginosa alkaline phosphatase. Comparative studies on the inactivation of alkaline phosphatase by glutaraldehyde showed significant differences when the purified pro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-078
更新日期:1981-05-01 00:00:00
abstract::Bacterial culture based on the use of plate media is an effective method for investigating bacterial populations in the environment. To improve the culturability of bacteria from freshwater lakes, we developed a new medium, FW70, which contains sodium pyruvate, casamino acids, and lake water and is solidified using ge...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-019
更新日期:2010-04-01 00:00:00
abstract::Schizophyllum commune Fr. was shown, by light, scanning, and transmission electron microscopy, to be a destructive mycoparasite on several phytopathogenic and nematode-trapping fungi. The hyphae of S. commune coiled around host hyphae and fruiting structures and penetrated them by means of either unspecialized hyphae ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-131
更新日期:1978-07-01 00:00:00
abstract::In Neurospora crassa, heat shock results in the induction of 9 to 11 heat shock proteins (HSP), of which HSP80 is the most abundant and the first to be synthesized. The induction of HSP80 was investigated during normal growth (2% sucrose) and under sucrose starvation. Transfer of mycelium to a medium supplemented with...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-031
更新日期:1988-02-01 00:00:00
abstract::The open reading frame pp0053, which has a high homology with the sequence of mitochondrial sulfide dehydrogenase (HMT2) conferring cadmium tolerance in fission yeast, was amplified from Pseudomonas putida KT2440 and expressed in Escherichia coli JM109(DE3). The isolated and purified PP0053-His showed absorption spect...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-022
更新日期:2006-08-01 00:00:00
abstract::A microcinematographic analysis of the behaviour and movements of cells and cell masses in mated cultures (NC4 X VI2) of Dictyostelium discoideum indicates that a chemotactic process directs cell aggregation during macrocyst development. Zygote giant cells form before aggregation begins and act as the aggregation cent...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-221
更新日期:1979-12-01 00:00:00
abstract::Boron (B) is toxic to living cells at levels above a certain threshold. We isolated several B-tolerant bacterial strains from soil samples and studied them for possible mechanisms of B tolerance. 16S rRNA gene sequencing and comparative phylogenetic analysis demonstrated that the isolates belong to the following 6 gen...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-106
更新日期:2010-01-01 00:00:00
abstract::Staphylococcus aureus isolated from the cervix of an infertile woman was found to cause complete immobilization of human spermatozoa in vitro. Only the cell culture and cell-free supernatant showed immobilization activity, indicating that the sperm immobilization factor might be released extracellularly by the organis...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-032
更新日期:2009-07-01 00:00:00
abstract::The presence of any Listeria species in food may be an indicator of poor hygiene in food processing facilities. The biochemical identification of Listeria species is laborious and time consuming. Therefore, the development of novel identification methods that are rapid and simple to perform would be an asset. In this ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-054
更新日期:2010-08-01 00:00:00
abstract::Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, ho...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-059
更新日期:2009-09-01 00:00:00
abstract::alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-095
更新日期:1986-06-01 00:00:00
abstract::Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea pla...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0399
更新日期:2014-10-01 00:00:00
abstract::Lysis of mid-log phase cells of the Gram-positive bacterium, Corynebacterium sepedonicum, by a combination of lysozyme treatment and incubation with alkaline sodium dodecyl sulfate at 56 degrees C led to the recovery of a single plasmid. The plasmid was purified in CsCl density gradients, and its molecular weight esti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-116
更新日期:1986-08-01 00:00:00
abstract::Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to detect proteins induced in Frankia sp. strain ACN14a-tsr by root exudates of its symbiotic host, Alnus glutinosa. The 5 most prominent proteins were purified from 2-D gels and characterized by N-terminal sequencing. All of these proteins had a high p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-046
更新日期:2001-06-01 00:00:00
abstract::The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intra...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-022
更新日期:1978-02-01 00:00:00
abstract::In Salmonella typhimurium LT2 the F factor of Escherichia coli K-12 replicates normally but is repressed; Flac+ cells give no visible lysis on solid media with male-specific phages, low frequency transfer of Flac+ (0.001-0.007 per donor cell), few f2 infective centers (0.002-0.006 per cell), and they propagate male-sp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-184
更新日期:1983-09-01 00:00:00
abstract::Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-015
更新日期:1989-01-01 00:00:00
abstract::Eighteen yeast species of the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus, each one represented by its type strain, were investigated with the objective of evaluating their carotenoid composition. The pigments were extracted from yeast cells, quantified by high pressure liquid chromatography ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-068
更新日期:2007-08-01 00:00:00
abstract::Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-227
更新日期:1976-10-01 00:00:00
abstract::Samples of seawater, sediment, and the following invertebrates were collected from the Mediterranean Sea in the neighborhood of Alexandria, Egypt: sea urchins (Echinus spp.), clams (Tapes spp.), and wedge shells (Donax trunculus). A total of 165 samples were collected from November 1979 to July 1980. Average counts (p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-187
更新日期:1982-11-01 00:00:00
abstract::Saccharomyces cerevisiae is a good model with which to study the effects of morphologic differentiation on the ecological behaviour of fungi. In this work, 33 morphologic mutants of a natural strain of S. cerevisiae, obtained with UV mutagenesis, were selected for their streak shape and cell shape on rich medium. Two ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W06-132
更新日期:2007-02-01 00:00:00