Mechanism of boron tolerance in soil bacteria.

Abstract:

:Boron (B) is toxic to living cells at levels above a certain threshold. We isolated several B-tolerant bacterial strains from soil samples and studied them for possible mechanisms of B tolerance. 16S rRNA gene sequencing and comparative phylogenetic analysis demonstrated that the isolates belong to the following 6 genera: Arthrobacter, Rhodococcus, Lysinibacillus, Algoriphagus, Gracilibacillus, and Bacillus. These isolates exhibited B-tolerance levels of 80, 100, 150, 300, 450, and 450 mmol/L, respectively, whilst maintaining a significantly lower intracellular B concentration than in the medium. Statistical analysis showed a negative correlation between the protoplasmic B concentration and the degree of tolerance to a high external B concentration. The kinetic assays suggest that the high B efflux and (or) exclusion are the tolerance mechanisms against a high external B concentration in the isolated bacteria.

journal_name

Can J Microbiol

authors

Ahmed I,Fujiwara T

doi

10.1139/w09-106

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

22-6

issue

1

eissn

0008-4166

issn

1480-3275

pii

w09-106

journal_volume

56

pub_type

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