Abstract：
:Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea plants for their potential use as biofertilizers. After obtaining a collection of 864 bacterial isolates, we performed a screen using the Salkowski reaction for the presence of auxin compounds (such as IAA) in bacterial Luria-Bertani supernatant (BLBS). Our results demonstrate that the Salkowski reaction has a greater specificity for detecting IAA than other tested auxins. Ten bacterial isolates displaying a wide range of auxin accumulation were selected, producing IAA levels of 5 to 90 μmol/L (according to the Salkowski reaction). Bacterial isolates were identified on the basis of 16S rDNA partial sequences: 9 isolates belonged to Enterobacter, and 1 isolate was classified as Serratia. The effect of BLBS on root morphology was evaluated in Arabidopsis thaliana. IAA production by rhizobacteria was confirmed by means of a DR5::GFP construct that is responsive to IAA, and also by HPLC-GC/MS. Finally, we observed that IAA secreted by rhizobacteria (i) modified the root architecture of A. thaliana, (ii) caused an increase in chickpea root biomass, and (iii) activated the green fluorescent protein (GFP) reporter gene driven by the DR5 promoter. These findings provide evidence that these novel bacterial isolates may be considered as putative plant-growth-promoting rhizobacteria modifying root architecture and increasing root biomass.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Fierro-Coronado RA,Quiroz-Figueroa FR,García-Pérez LM,Ramírez-Chávez E,Molina-Torres J,Maldonado-Mendoza IEdoi
10.1139/cjm-2014-0399subject
Has Abstractpub_date
2014-10-01 00:00:00pages
639-48issue
10eissn
0008-4166issn
1480-3275journal_volume
60pub_type
杂志文章abstract：:We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library se...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-064
更新日期：2006-11-01 00:00:00
abstract：:The study investigated the presence of Borrelia-specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG pos...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-105
更新日期：2007-12-01 00:00:00
abstract：:Fetal bovine serum (FBS) is a necessary constituent of the culture media employed to foster the growth of Trypanosoma cruzi epimastigote forms. In different laboratories, the serum is used at final concentrations of 5 or 10%. We have normally supplemented the complex medium with 10% FBS. Under this condition we have d...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-132
更新日期：1995-10-01 00:00:00
abstract：:Lyme disease is a tick-borne disease that is emerging in Canada. The disease is caused by spirochetes of the Lyme borreliosis group, which is expanding as new species are discovered. In Canada, Lyme disease risk has so far been assessed primarily by detection of Borrelia burgdorferi sensu stricto. Of Ixodes scapularis...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0376
更新日期：2019-02-01 00:00:00
abstract：:A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-059
更新日期：1997-05-01 00:00:00
abstract：:Effects of Trichoderma harzianum and T. polysporum on chlamydospore production by two isolates of Phytophthora cinamomi were determined over a 21-day period in nonsterile, sterilized, and sterilized amended soil. Trichoderma was either coinoculated with P. cinnamomi or added to the cultures on day 3 of the incubation ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-042
更新日期：1977-03-01 00:00:00
abstract：:During exponential growth, strain AW405 of Escherichia coli K-12 did not regulate the fatty acid composition of its lipids in response either to temperature or to the addition of NaCl, KCl, or MgCl2 to the medium. Growth was severely restricted at temperatures below 21 degrees C. Differential scanning calorimetry (DSC...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-128
更新日期：1981-08-01 00:00:00
abstract：:An endo-beta-1,4-xylanase gene was cloned from Neocallimastix patriciarum 27 in the bacteriophage vector lambda gtWES lambda B and was subcloned into the plasmid vectors pUC18 and pUC19 in which xylanase activity was expressed in both orientations. The xylanase was located in the periplasmic space of the host, Escheri...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-020
更新日期：1993-01-01 00:00:00
abstract：:The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation pat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-090
更新日期：1990-07-01 00:00:00
abstract：:The possibility of using the volatile-producing fungus Muscodor albus for biofumigation against building molds was investigated. Several species of Aspergillus and Penicillium as well as fungi belonging to nine other genera were inhibited or killed in vitro by volatiles produced by potato dextrose agar or rye grain cu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W06-124
更新日期：2007-03-01 00:00:00
abstract：:Through genomic analysis of mucosa-associated Escherichia coli strains, we found a close genetic association among isolates from pediatric inflammatory bowel disease (IBD) patients. A specific E. coli pathovar, adherent-invasive E. coli (AIEC), was found in Crohn's disease (CD) adult patients - this pathovar has enhan...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-007
更新日期：2012-04-01 00:00:00
abstract：:Members of the genus Bacillus and related spore-forming genera are ubiquitous. However, Bacillus-like species isolated from marine sediments have attracted less interest than their terrestrial relatives. Here, we investigated the diversity of Bacillus-like bacterial communities in the sediments of the Bamenwan mangrov...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0449
更新日期：2017-03-01 00:00:00
abstract：:The ability to rapidly identify and quantify a microbial strain in a complex environmental sample has widespread applications in ecology, epidemiology, and industry. In this study, we describe a rapid method to obtain functionally specific genetic markers that can be used in conjunction with standard or real-time poly...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-140
更新日期：2006-05-01 00:00:00
abstract：:Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-063
更新日期：1982-04-01 00:00:00
abstract：:Antibiotic-resistant and antimetabolite-resistant mutants of the nitrogen-fixing symbiotic bacterium Frankia were isolated to provide strains with genetic backgrounds amenable to genetic analysis. The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV light on four Frankia strains were investigated. U...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-013
更新日期：2004-04-01 00:00:00
abstract：:To screen, isolate, and characterize bacterial populations producing microcin J25, we report here two rapid, reliable, and sensitive methods, using polymerase chain reaction and colony blot hybridization with a digoxigenin-labelled probe. A sample of 26 Escherichia coli strains isolated from poultry intestinal content...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:Of 24 bacterial species examined for lisinopril refractive peptidyl dipeptidase activity, only 8 contained activity. Activity in Pseudomonas maltophilia was more than fourfold higher than that of any other species. Pseudomonas maltophilia may be unique among bacteria in possessing high peptidyl dipeptidase activity th...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-010
更新日期：1990-01-01 00:00:00
abstract：:Antibiotic susceptibilities of 382 strains of Campylobacter jejuni isolated in Alberta, Canada, in 1980 and 1981 were determined. Although none were resistant to erythromycin or gentamicin, 5.4 and 22% of strains were resistant to ampicillin in 1980 and 1981, respectively. Tetracycline resistance was noted in 6.8% of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-006
更新日期：1986-01-01 00:00:00
abstract：:In the present study, the effect of temperature and water activity on fungal growth and ochratoxin production on coffee-based medium was assessed. Optimal growth of three Aspergillus strains was observed in the same ecological conditions, namely 30 degrees C and 0.99 water activity. Maximal daily growth is 11.2, 6.92,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W06-143
更新日期：2007-07-01 00:00:00
abstract：:Haemophilus paragallinarum secretes metalloproteases into different culture media lacking serum. Secreted proteins, concentrated by precipitation with 70% ammonium sulphate ((NH(4))(2)SO(4)) or methanol, displayed proteolytic activity at >100 kDa molecular mass in 10% polyacrylamide gels co-polymerized with porcine ge...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-067
更新日期：2005-10-01 00:00:00
abstract：:In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-188
更新日期：1979-10-01 00:00:00
abstract：:The antibacterial mechanism of a Cinnamomum cassia essential oil from Vietnam and of its main component (trans-cinnamaldehyde, 90% (m/m) of C. cassia essential oil) against a Listeria innocua strain was investigated to estimate their potential for food preservation. In the presence of C. cassia essential oil or trans-...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0481
更新日期：2015-04-01 00:00:00
abstract：:A field site near Humboldt, Saskatchewan, was annually treated with hog or cattle manure and cropped to canola, spring wheat, barley, and canola from 1997 to 2000. During each growing season, soil was analyzed for microbial populations in terms of activity and community structure, and crops were assessed for root rot ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-069
更新日期：2003-09-01 00:00:00
abstract：:The search for new antimicrobial compounds involves finding novel sources of chemotherapeutic compounds or manipulating and combining structures from existing molecules. Small antimicrobial peptides (AMPs) are components of innate immune defenses characterized in greatest detail in insect-derived AMPs. We have generat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-001
更新日期：2009-05-01 00:00:00
abstract：:The presence of any Listeria species in food may be an indicator of poor hygiene in food processing facilities. The biochemical identification of Listeria species is laborious and time consuming. Therefore, the development of novel identification methods that are rapid and simple to perform would be an asset. In this ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-054
更新日期：2010-08-01 00:00:00
abstract：:We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-126
更新日期：1992-08-01 00:00:00
abstract：:The mouse model of intraperitoneal sepsis with Proteus rettgeri was used to evaluate the anti-endotoxic effect of polymyxin B sulfate. An unexpected reversal of the usual protective effect of polymyxin in experimental enterobacterial sepsis was observed in which the lethality of the infection was enhanced. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-039
更新日期：1980-02-01 00:00:00
abstract：:Actinomycetes DNAs were digested with restriction enzymes to study the presence of methylated bases. Analysis showed that the enterobacterial Dam and Dcm systems are absent. Methylation at the internal cytosine in CCGG sequences, typical of eukaryotes, was also absent. We also tested 18 restriction endonucleases recog...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-031
更新日期：1996-02-01 00:00:00
abstract：:A species of Arthrobacter was recovered during culture of the causal organism of pitch canker of southern pines. Fusarium moniliforme var. subglutinans (FMS). Arthrobacter is a relatively common soil bacterium and is lytic to several fungal pathogens in the soil. Soil samples from two seed orchards with pitch canker a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-004
更新日期：1981-01-01 00:00:00
abstract：:The immune benefits associated with the optimal intake of dietary fatty acids are widely known. The objective of the present investigation was to elucidate the role of omega-3 polyunsaturated fatty acids (n-3 PUFA) food source on acute pneumonia induced by Klebsiella pneumoniae. Three different n-3 PUFA preparations (...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0521
更新日期：2013-07-01 00:00:00
