Isolation of antibiotic-resistant and antimetabolite-resistant mutants of Frankia strains EuI1c and Cc1.17.

Abstract:

:Antibiotic-resistant and antimetabolite-resistant mutants of the nitrogen-fixing symbiotic bacterium Frankia were isolated to provide strains with genetic backgrounds amenable to genetic analysis. The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV light on four Frankia strains were investigated. UV irradiation or EMS treatment of strain EuI1c cells resulted in the formation of two different colony types: rough and smooth. The smooth colonies were conditional sporulation mutants. In the case of EMS-induced cells of strain Cc1.17, resistance to lincomycin, ampicillin, and 5-fluorouracil occurred at a frequency of 1 x 10(-5), 1 x 10(-5), and 4 x 10(-5), respectively. The lincomycin-resistant mutants produced a yellow-tan pigment that was released into the growth medium. Resistance to tetracycline and lincomycin with EMS-induced cells of strain EuI1c occurred at a frequency of 3.2 x 10(-3) and 4.7 x 10(-4), respectively. These strains will be useful for the development of genetic methods for Frankia.

journal_name

Can J Microbiol

authors

Myers AK,Tisa LS

doi

10.1139/w04-013

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

261-7

issue

4

eissn

0008-4166

issn

1480-3275

pii

w04-013

journal_volume

50

pub_type

杂志文章
  • B subunit of Escherichia coli heat-labile enterotoxin as adjuvant of humoral immune response in recombinant BCG vaccination.

    abstract::The B subunit of Escherichia coli heat-labile enterotoxin (LTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral adjuvant that induces a strong immune response against co-administered or coupled antigens. In this paper, the effect of LTB on the humoral immune response to rec...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-056

    authors: da Silva Ramos Rocha A,Conceição FR,Grassmann AA,Lagranha VL,Dellagostin OA

    更新日期:2008-08-01 00:00:00

  • Inhibition of uridine 5'-diphosphate-N-acetylmuramyl-L-alanine synthetase by beta-chloro-L-alanine in Escherichia coli.

    abstract::The synthesis of the nucleotide precursors for peptidoglycan is regulated by the relA gene in Escherichia coli. Thus, nucleotide precursors labeled with [3H]diaminopimelic acid accumulated in a relA strain but not in an isogenic relA+ strain during amino acid deprivation. Furthermore, nucleotide precursor synthesis wa...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m82-098

    authors: Ishiguro EE

    更新日期:1982-06-01 00:00:00

  • Comparison of thermostable macromolecular antigens from leprosy-associated bacteria.

    abstract::Three types of bacteria are associated with leprosy: Mycobacterium leprae, leprosy-derived corynebacteria (LDC), and armadillo-derived mycobacteria (ADM). The immunological relationships between these three types of bacteria and Mycobacterium bovis BCG, used as a reference, were determined by cross-immunoelectrophores...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m94-083

    authors: Baelden MC,Bouckaert AE,Grégoire D,van de Poele M,Coene M

    更新日期:1994-06-01 00:00:00

  • [Influence of anaerobiosis on the sterol composition of Mucor hiemalis].

    abstract::Strict anaerobic cultures of Mucor hiemalis lead to major modifications of the quantitative and qualitative composition of sterols. Sterols of which the lateral chain contains an ethyl or ethylidene group in C24 are for the first time brought to evidence in a Mucorales. Isolation in the same conditions of E-ethylidene...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-095

    authors: Herber R,Villoutreix J,Granger P,Chapelle S

    更新日期:1983-05-01 00:00:00

  • Characterization of the mitogenic activity elicited by Neisseria gonorrhoeae ribosomal fractions.

    abstract::Ribosomal preparations from Neisseria gonorrhoeae types 1 and 4 were examined for their in vitro stimulation of mouse splenocytes to determine the ribosomal moiety or contaminant responsible for the immunoproliferative activity. In immunodiffusion tests with homologous rabbit antiserum, crude 70S ribosomes formed four...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-094

    authors: Brodeur BR

    更新日期:1978-05-01 00:00:00

  • Characteristics of the rhizobia associated with Dalea spp. in the Ordway, Kellogg-Weaver Dunes, and Hayden prairies.

    abstract::Habitat fragmentation affects the biodiversity and function of aboveground organisms in natural ecosystems but has not been studied for effects on belowground species. In this paper, we consider the diversity of the rhizobia associated with the indigenous legume Dalea purpurea in 3 residual prairie areas in Minnesota ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-107

    authors: Tlusty B,van Berkum P,Graham PH

    更新日期:2005-01-01 00:00:00

  • Cold shock proteins and cold acclimation proteins in the psychrotrophic bacterium Pseudomonas putida Q5 and its transconjugant.

    abstract::The production of cold shock proteins (csps) and cold acclimation proteins (caps) was characterized in the psychrotrophic bacterium Pseudomonas putida Q5 and its transconjugant P. putida Q5T which contains the toluene-degradative TOL (pWWO) plasmid, using two-dimensional gel electrophoresis and computing scanning lase...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-100

    authors: Gumley AW,Inniss WE

    更新日期:1996-08-01 00:00:00

  • Cytosolic and membrane-bound chitinases of two mucoraceous fungi: a comparative study.

    abstract::Chitinases isolated from membrane and cytosolic fractions of two mucoraceous fungi, Choanephora cucurbitarum and Phascolomyces articulosus, were investigated. The membrane-bound chitinase was isolated by Bio-Gel P-100 and DEAE Bio-Gel A chromatographic techniques. On SDS-PAGE the chitinase from both fungi migrated as ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-056

    authors: Balasubramanian R,Manocha MS

    更新日期:1992-04-01 00:00:00

  • Accelerated detection of lymphocytic choriomeningitis virus in diagnostic specimens.

    abstract::Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for st...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-245

    authors: Lewis VJ,Thacker WL,Mitchell SH

    更新日期:1976-11-01 00:00:00

  • Characterization of a plasmid in isolates of Corynebacterium sepedonicum.

    abstract::Lysis of mid-log phase cells of the Gram-positive bacterium, Corynebacterium sepedonicum, by a combination of lysozyme treatment and incubation with alkaline sodium dodecyl sulfate at 56 degrees C led to the recovery of a single plasmid. The plasmid was purified in CsCl density gradients, and its molecular weight esti...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-116

    authors: Clark MC,Lawrence CH

    更新日期:1986-08-01 00:00:00

  • A technique for preserving aerial fungal structures for scanning electron microscopy.

    abstract::A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-106

    authors: King EJ,Brown MF

    更新日期:1983-06-01 00:00:00

  • Kodamaea kakaduensis and Candida tolerans, two new ascomycetous yeast species from Australian Hibiscus flowers.

    abstract::Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w98-225

    authors: Lachance MA,Bowles JM,Starmer WT,Barker JS

    更新日期:1999-02-01 00:00:00

  • Suitability of membrane-filter techniques to study the ultrastructure of Fusarium solani in soil.

    abstract::The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-239

    authors: Van Eck WH

    更新日期:1976-11-01 00:00:00

  • Prevalence of Giardia and Cryptosporidium and characterization of Cryptosporidium spp. isolated from wildlife, human, and agricultural sources in the North Saskatchewan River Basin in Alberta, Canada.

    abstract::The environmental distribution of Giardia spp. and Cryptosporidium spp. is dependent upon human, agricultural, and wildlife sources. The significance of each source with regard to the presence of parasites in the environment is unknown. This 2-year study examined parasite prevalence in human sewage influent, wildlife,...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w02-047

    authors: Heitman TL,Frederick LM,Viste JR,Guselle NJ,Morgan UM,Thompson RC,Olson ME

    更新日期:2002-06-01 00:00:00

  • Menadione reductase from Clostridium tyrobutyricum.

    abstract::Menadione reductase (EC 1.6.99.2) has been purified 67-fold from Clostridium tyrobutyricum extracts. The molecular weight was found to be 60 000 and the prosthetic group was identified as FMN on the basis of the enzymatic analysis data. The binding of FMN to the menadione dehydrogenase apoenzyme was relatively weak wi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-053

    authors: Petitdemange H,Marczak R,Raval G,Gay R

    更新日期:1980-03-01 00:00:00

  • Cryptococcus festucosus sp. nov. a new hymenomycetous yeast in the Holtermannia clade.

    abstract::Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-099

    authors: Golubev WI,Sampaio JP,Alves L,Golubev NW

    更新日期:2004-12-01 00:00:00

  • [Increase of resistance to Pseudomonas aeruginosa infection in mice caused by Staphylococcus aureus].

    abstract::In our study of opportunistic pathogens, we have some indication that Staphylococcus aureus can increase resistance in mice against Pseudomonas aeruginosa. Intraperitoneal injections of sublethal doses of S. aureus had a protective effect in mice against lethal doses of P. aeruginosa, more so if living and coagulase-p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: deRepentigny J,Mathieu LG,Coallier-Ascah J

    更新日期:1976-07-01 00:00:00

  • Purification and characterization of a psychrophilic catalase from Antarctic Bacillus.

    abstract::Catalase from Bacillus sp. N2a (BNC) isolated from Antarctic seawater was purified to homogeneity. BNC has a molecular mass of about 230 kDa and is composed of four identical subunits of 56 kDa. The catalase showed optimal activity at 25 degrees C and at a pH range of 6-11. The enzyme could be inhibited by azide, hydr...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-066

    authors: Wang W,Sun M,Liu W,Zhang B

    更新日期:2008-10-01 00:00:00

  • Trichosporon guehoae sp.nov., an anamorphic basidiomycetous yeast.

    abstract::A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w99-055

    authors: Middelhoven WJ,Scorzetti G,Fell JW

    更新日期:1999-08-01 00:00:00

  • DNA base composition, nature of intracellular DNA, morphology, and classification of bacteriophages infecting Micrococcus luteus.

    abstract::Ten bacteriophages infecting Micrococcus luteus have been characterized. All phages contain double-stranded DNA, of 64.3--73.5 mol% guanine plus cytosine (GC). The DNA of phage N7 has the highest GC content reported for any bacterial virus. No unusual bases have been found. The intracellular replicating DNAs of six ph...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-158

    authors: Compton SW,Mayo JA,Ehrlich M,Ackermann HW,Tremblay L,Cords CE,Scaletti JV

    更新日期:1979-09-01 00:00:00

  • Transforming the sapstaining fungus Ophiostoma piceae with Agrobacterium tumefaciens.

    abstract::A transformation protocol mediated by Agrobacterium tumefaciens is described for the sapstaining fungus Ophiostoma piceae. We compared transformants obtained from Agrobacterium with those obtained from yeast-like cells made into spheroplasts and treated with CaCl2. For all putative transformants analyzed, Southern hyb...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-036

    authors: Tanguay P,Breuil C

    更新日期:2003-04-01 00:00:00

  • Isolation of lactic acid bacteria from swine milk and characterization of potential probiotic strains with antagonistic effects against swine-associated gastrointestinal pathogens.

    abstract::Probiotics are usually isolated from the gastrointestinal tract of humans and animals. The search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. To our knowledge, no reports regarding the potential probiotic effect of bac...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2015-0811

    authors: Quilodrán-Vega SR,Villena J,Valdebenito J,Salas MJ,Parra C,Ruiz A,Kitazawa H,García A

    更新日期:2016-06-01 00:00:00

  • Effect of some environmental parameters on fermentative hydrogen production by Enterobacter cloacae DM11.

    abstract::Fermentative hydrogen production was carried out by Enterobacter cloacae DM11, using glucose as the substrate. The effects of initial substrate concentration, initial medium pH, and temperature were investigated. Results showed that at an initial glucose concentration of 1.0% (m/v), the molar yield of hydrogen was 3.3...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w06-005

    authors: Nath K,Kumar A,Das D

    更新日期:2006-06-01 00:00:00

  • Lethal effect of polymyxin B sulfate in experimental Proteus rettgeri infection in mice.

    abstract::The mouse model of intraperitoneal sepsis with Proteus rettgeri was used to evaluate the anti-endotoxic effect of polymyxin B sulfate. An unexpected reversal of the usual protective effect of polymyxin in experimental enterobacterial sepsis was observed in which the lethality of the infection was enhanced. ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-039

    authors: Bannatyne RM,Cheung R

    更新日期:1980-02-01 00:00:00

  • Characterization of diacetin B, a bacteriocin from Lactococcus lactis subsp. lactis bv. diacetylactis UL720.

    abstract::Fourteen Lactococcus lactis strains showing inhibitory activity against Listeria innocua SICC 4202 were isolated from different French raw milks and raw milk cheeses and screened for bacteriocin production by the triple layer method under conditions that eliminate the effects of lactic acid and hydrogen peroxide. Thre...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m95-114

    authors: Ali D,Lacroix C,Thuault D,Bourgeois CM,Simard RE

    更新日期:1995-09-01 00:00:00

  • The influence of season and stage of development of plant on Endogone mycorrhiza of field-grown wheat.

    abstract::A quantitative and qualitative survey of the indigenous Endogone population in wheat field soil and vesicular-arbuscular mycorrhiza in wheat roots as influenced by season and by the stage of development of the wheat plant was made. The number of Endogone spores in wheat field soil remained relatively unchanged in win...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-151

    authors: Saif SR,Khan AG

    更新日期:1975-07-01 00:00:00

  • Effects of Gypsophila saponins on bacterial growth kinetics and on selection of subterranean clover rhizosphere bacteria.

    abstract::Plant secondary metabolites, such as saponins, have a considerable impact in agriculture because of their allelopathic effects. They also affect the growth of soil microorganisms, especially fungi. We investigated the influence of saponins on rhizosphere bacteria in vitro and in soil conditions. The effects of gypsoph...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-052

    authors: Fons F,Amellal N,Leyval C,Saint-Martin N,Henry M

    更新日期:2003-06-01 00:00:00

  • Impact of liquid hog manure applications on antibiotic resistance genes concentration in soil and drainage water in field crops.

    abstract::Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteri...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2019-0343

    authors: Larouche É,Généreux M,Tremblay MÈ,Rhouma M,Gasser MO,Quessy S,Côté C

    更新日期:2020-10-01 00:00:00

  • Evaluation of nitrogenous media components by Plackett-Burman statistical design for beta-d-fructofuranosidase production by Saccharomyces sp. strain GVT263.

    abstract::beta-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-146

    authors: Venkateshwar M,Chaitanya K,Altaf MD,Hameeda B,Ghopal Reddy M

    更新日期:2009-04-01 00:00:00

  • The metabolism of carbohydrates by extremely halophilic bacteria: identification of galactonic acid as a product of galactose metabolism.

    abstract::Cell-free extracts prepared from the extremely halophilic bacterium Halobacterium saccharovorum oxidize galactose and accumulate a product which reacts as if it were a lactone. The product does not act as a reducing sugar and contains all six of the carbon atoms initially present in galactose. The product was jugged t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-174

    authors: Hochstein LI,Dalton BP,Pollock G

    更新日期:1976-08-01 00:00:00