Abstract：
:Habitat fragmentation affects the biodiversity and function of aboveground organisms in natural ecosystems but has not been studied for effects on belowground species. In this paper, we consider the diversity of the rhizobia associated with the indigenous legume Dalea purpurea in 3 residual prairie areas in Minnesota and Iowa. Using Dalea purpurea as a trap host, 218 rhizobia were recovered from these soils then characterized using BOXA1R PCR. Three major and 13 minor groups were distinguished based on a similarity of greater than 75% in fingerprint patterns. Each major group consisted almost exclusively of rhizobia from a single prairie, with the diversity of Dalea rhizobia recovered from the Hayden Prairie less than that obtained with rhizobia from the other prairies. Based on 16S rRNA gene sequence analysis, isolates from the Hayden, Ordway, and Kellogg-Weaver Dunes prairies were most similar to Rhizobium etli and Rhizobium leguminosarum, Rhizobium gallicum, and Mesorhizobium amorphae and Mesorhizobium huakuii, respectively. This variation in the dominant microsymbiont species across the 3 prairies studied was unexpected but could have been influenced by the limited number of samples that we were allowed to take, by unanticipated cross-nodulation between native legumes, and by variation in the range of legume species present in each residual prairie area. While some of the rhizobia from Dalea nodulated Phaseolus vulgaris, Macroptilium atropurpureum, Leucaena leucocephala, and Onobrychis viciifolia in addition to the Dalea species tested, others nodulated Astragalus canadensis or Amorpha canescens.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Tlusty B,van Berkum P,Graham PHdoi
10.1139/w04-107subject
Has Abstractpub_date
2005-01-01 00:00:00pages
15-23issue
1eissn
0008-4166issn
1480-3275pii
w04-107journal_volume
51pub_type
杂志文章abstract：:The poly(3-hydroxybutyrate) (PHB) depolymerase structural gene of Comamonas sp. (phaZCsp) was cloned in Escherichia coli and identified by halo formation on PHB-containing solid medium. The nucleotide sequence of a 1719 base pair MboI fragment was determined and contained one large open reading frame (ORF1, 1542 base ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-183
更新日期：1995-01-01 00:00:00
abstract：:Listeria grayi is a nonpathogenic Gram-positive bacterium that demonstrates considerable similarities to other members in the genus Listeria, including the foodborne human pathogen Listeria monocytogenes and the animal pathogen Listeria ivanovii. A rapid diagnostic test to identify and diagnose listeriosis would be va...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-108
更新日期：2005-01-01 00:00:00
abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-102
更新日期：2007-01-01 00:00:00
abstract：:The catalytic subunit of many Escherichia coli redox enzymes bares a twin-arginine translocation (Tat)-dependent signal peptide in its precursor, which directs the redox enzyme complex to this Sec-independent pathway. NarG of the E. coli nitrate reductase NarGHI complex possesses a vestige twin-arginine motif at its N...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-111
更新日期：2009-02-01 00:00:00
abstract：:In a previous study it was shown that the number of wood-inhabiting bacteria was drastically reduced after colonization of beech (Fagus sylvatica) wood blocks by the white-rot fungus Hypholoma fasciculare, or sulfur tuft (Folman et al. 2008). Here we report on the mechanisms of this fungal-induced antibacterial activi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-023
更新日期：2010-05-01 00:00:00
abstract：:Vero cells were more suitable then BHK-21 cells for the detection of rubella-specific IgG antibodies because the nonspecific fluorescence was minimal. However, BHK-21 cells were found more sensitive than Vero cells for the detection of rubella-specific IgM antibodies. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-041
更新日期：1975-03-01 00:00:00
abstract：:Canadian oil sands tailings are predominately sodic residues contaminated by hydrocarbons such as naphthenic acids. These conditions are harsh for plant development. In this study, we evaluated the effect of inoculating roots of Alnus viridis ssp. crispa and Alnus incana ssp. rugosa with ectomycorrhizal fungi in the p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0703
更新日期：2016-07-01 00:00:00
abstract：:The aim of the present study was to explore a new approach based on the hemagglutination (HA) assay to understand the colonization ability of Shigella spp. To study colonization ability, an animal model of 4-day-old suckling mouse, was exploited. We characterized the HA activity of 48 Shigella strains, with erythroc...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-095
更新日期：2012-10-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-084
更新日期：1993-06-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-109
更新日期：1980-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-170
更新日期：1985-10-01 00:00:00
abstract：:The comparative ability of different methods to assess virulence of Listeria species was investigated in ten Listeria strains. All strains were initially subjected to pulsed-field gel electrophoresis analysis to determine their relatedness. Virulence characteristics were subsequently tested for by (i) determining the ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-129
更新日期：2006-04-01 00:00:00
abstract：:Mycobacterium sp. strain KMS utilizes pyrene, a high-molecular weight polycyclic aromatic hydrocarbon (PAH), as a sole carbon source. Bioinformatic analysis of the genome of isolate KMS predicted 25 genes with the potential to encode 17 pyrene-induced proteins identified by proteomics; these genes were clustered on b...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-093
更新日期：2012-01-01 00:00:00
abstract：:Pseudomonas maltophilia has been identified in the rhizosphere of several cultured plants: cabbage, rape, mustard, corn, beet. In comparison with nonrhizospheric soil, all the rhizospheric samples analysed contain more of this Pseudomonas and the most important stimulation is supplied by cruciferous plants. The possib...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:Caulobacters are biofilm-forming members of the natural flora of soil and aquatic environments, which exhibit several characteristics that make them attractive for development of high surface area microbial bioreactors or biosensors. Although caulobacters are well characterized genetically, little is known about their...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:Epiphytic tracer bacteris (a Pseudomonas sp. and an Arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. Of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. Seed from two of these pods also carried tracers. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-064
更新日期：1976-03-01 00:00:00
abstract：:Scanning and transmission electron microscopy was applied for a morphological study of three strains of Bifidobacterium grown on solid or liquid media. The pronounced pleomorphism of the cultures previously observed by light microscopy was confirmed. A possible sequence of the morphological events during transformatio...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-197
更新日期：1975-09-01 00:00:00
abstract：:Experiments were conducted to examine the dynamics of Cache Valley virus in Culiseta inornata, the probable chief vector of the virus. Of about 1500 laboratory reared C. inornata exposed to viraemic suckling mice, 72 took a blood meal. A relatively high precentage (93%) of the latter mosquitoes became infected. The vi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:Cellobiose transport by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes was measured using randomly tritiated cellobiose. When assayed at the same concentration (1 mM), total cellobiose uptake was one-fourth to one-third that of total glucose uptake. The abilities of F. succinogenes to transpo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-021
更新日期：1991-02-01 00:00:00
abstract：:A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurosp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-147
更新日期：1991-11-01 00:00:00
abstract：:A transformation protocol mediated by Agrobacterium tumefaciens is described for the sapstaining fungus Ophiostoma piceae. We compared transformants obtained from Agrobacterium with those obtained from yeast-like cells made into spheroplasts and treated with CaCl2. For all putative transformants analyzed, Southern hyb...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-036
更新日期：2003-04-01 00:00:00
abstract：:Fecal coliforms (FC) counts were compared with Escherichia coli counts in differently contaminated freshwater samples (n = 166). FC were enumerated by plate count on triphenyl 2,3,5-tetrazolium chloride Tergitol medium. Escherichia coli were enumerated by the most probable number microplate method based on the detecti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-033
更新日期：2007-06-01 00:00:00
abstract：:Corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. Corynebacterium glutamicum his genes are located and transcribed in two unlinked loci, hisEG and hisDCB-orf1-orf2-hisHA-impA-hisFI. The latter his operon starts the transcription at the C residue localized...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-115
更新日期：2010-02-01 00:00:00
abstract：:The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-131
更新日期：2005-03-01 00:00:00
abstract：:The prevalence of Coxiella burnetti infection (Q fever) was determined among Nova Scotia (N.S.) and Prince Edward Island (P.E.I.) blood donors by using the complement fixation and microimmunofluorescence (IF) test. The complement fixation and IF antibody tests measured antibody prevalence for the phase II or phase I a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-021
更新日期：1984-01-01 00:00:00
abstract：:Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease is spread through this geographical region, particularly in countries that border those that are almost free of Mycobacterium bovis. In this work, we have analyzed the spacer oligonucl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-083
更新日期：2005-11-01 00:00:00
abstract：:Pseudomonas aeruginosa ATCC 9027 grew on 0.5% (v/v) hexadecane as a sole carbon source in a chemically defined medium which required the addition of Fe3+ and Ca2+. There was a variable and extended lag period before an active growth rate was attained. Visible light microscopic evidence revealed that the bacteria did n...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-049
更新日期：1986-03-01 00:00:00
abstract：:Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal comple...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-085
更新日期：2005-07-01 00:00:00
abstract：:Listeria monocytogenes and Listeria innocua differ markedly in virulence but are indistinguishable by classical taxonomic criteria. Both species are actively motile and produce abundant flagellin at 22 degrees C. We have found, however, noticeable differences between L. monocytogenes and L. innocua in motility and fla...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-076
更新日期：1995-07-01 00:00:00
abstract：:Heat activation (70 degrees C for 20 min) resulted in alteration in structural proteins and enzymes found in Bacillus cereus spore coats. The three notable changes were increased glycosylation of coat proteins, alteration in polypeptide pattern on sodium dodecyl sulfate - polyacrylamide gels, and an increase in free S...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-062
更新日期：1981-04-01 00:00:00
