Abstract：
:HI plasmids are distinguished by their thermosensitive mode of conjugation (transfer efficiency is optimal at 22-30 degrees C) and their capacity to encode multiple antibiotic resistance. These traits have implicated HI plasmids as potential vectors in the dissemination of antibiotic resistance among pathogenic and indigenous bacterial species in water and soil environments. We compared the transfer efficiency of HI plasmids with that of plasmids from 13 other incompatibility groups at 37, 24, and 14 degrees C in intragenic conjugations between laboratory strains of Escherichia coli K-12 under in vitro conditions. Only the HI plasmids and a representative plasmid from incompatibility groups M, N, P alpha, T, and W were observed to be transmissible at 14 degrees C. These plasmids, along with HI plasmids and the related HII representative, were tested for their host range and transfer proficiency to Enterobacteria species and some other Gram-negative organisms of environmental significance at 24 and 14 degrees C. Notable differences in the host range of HI plasmids compared with plasmid representatives from the other enterobacterial groups were not evident at 24 degrees C. At 14 degrees C, R478 (HI2) displayed the broadest host range and transfer proficiency among the test plasmids. The ability of several plasmid groups, including HI, to transfer at 14-24 degrees C to Vibrio cholerae non 01, Salmonella typhi, and the fish pathogens Aeromonas salmonicida, Vibrio anguillarum, and Yersinia ruckeri needs to be corroborated by in situ studies.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Maher D,Taylor DEdoi
10.1139/m93-084subject
Has Abstractpub_date
1993-06-01 00:00:00pages
581-7issue
6eissn
0008-4166issn
1480-3275journal_volume
39pub_type
杂志文章abstract：:Antibiotic-resistant and antimetabolite-resistant mutants of the nitrogen-fixing symbiotic bacterium Frankia were isolated to provide strains with genetic backgrounds amenable to genetic analysis. The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV light on four Frankia strains were investigated. U...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-013
更新日期：2004-04-01 00:00:00
abstract：:Arsenic hyperaccumulator fern Pteris vittata L. produces large amounts of root exudates that are hypothesized to solubilize arsenic and maintain a unique rhizosphere microbial community. Total heterotrophic counts on rich or defined media supplemented with up to 400 mmol/L of arsenate showed a diverse arsenate-resista...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-005
更新日期：2010-03-01 00:00:00
abstract：:The effects of inhibition of protein synthesis on the cell size distributions of rel+ and relA- derivatives of Escherichia coli K-12 were determined. Amino acid deprivation resulted in a reduction in the cell sizes of rel+ strains but not of relA- strains. Treatment with chloramphenicol (CAM) did not alter the size di...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-127
更新日期：1978-06-01 00:00:00
abstract：:To develop a library-dependent method of tracking fecal sources of contamination of beaches on the Atlantic coast of southwestern France, a library of 6368 Escherichia coli isolates was constructed from samples of feces, from 40 known human or animal sources collected in the vicinity of Arcachon Bay in 2010, and in Fr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0244
更新日期：2020-12-01 00:00:00
abstract：:Sixty-four species of filamentous fungi from five flare pits in northern and western Canada were tested for their ability to degrade crude oil using gas chromatographic analysis of residual hydrocarbons following incubation. Nine isolates were tested further using radiorespirometry to determine the extent of mineraliz...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Glutaraldehyde, the biological fixative of choice in the cytochemical localization of the phosphatases, was investigated for its effects on Pseudomonas aeruginosa alkaline phosphatase. Comparative studies on the inactivation of alkaline phosphatase by glutaraldehyde showed significant differences when the purified pro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-078
更新日期：1981-05-01 00:00:00
abstract：:The effects of postirradiation incubation with various chemicals structurally related to pantolactone on the survival of ultraviolet-irradiated Escherichia coli lon and ruv mutants were examined. Certain cyclic compounds with a butyrolactone, tetrahydrofuran, furan, tetrahydropyran, or 2-pyron ring showed substantial ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-254
更新日期：1980-12-01 00:00:00
abstract：:Repetitive element-polymerase chain reaction (REP-PCR) DNA fingerprinting and library-based microbial source tracking (MST) methods were utilized to investigate the potential sources of Escherichia coli pollution in recreational waters of southeastern Lake Huron. In addition to traditional sources such as humans, agri...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-123
更新日期：2009-03-01 00:00:00
abstract：:Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we scre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-062
更新日期：2006-10-01 00:00:00
abstract：:A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-151
更新日期：1977-08-01 00:00:00
abstract：:The rumen microbiota plays a major role in the metabolism and absorption of indigestible food sources. Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries) are important ruminant species for animal husbandry in the Tarim Basin. However, the microbiota and rum...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0596
更新日期：2017-05-01 00:00:00
abstract：:This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-001
更新日期：1983-01-01 00:00:00
abstract：:Candida albicans biofilm formation is considered an important matter because it can lead to strong resistance to conventional antifungal agents. Hyphae formed by C. albicans can also act as an important virulence factor related to its biofilm. The objective of this study was to determine the effect of zerumbone, a mon...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0155
更新日期：2019-10-01 00:00:00
abstract：:Examination of immune complexes between cells of Streptococcus mutans and homologous antiserum by the techniques of thin-sectioning and freeze-etching revealed that the cells were embedded within and extensive matrix 80-90 nm thick with defined boundaries. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-129
更新日期：1976-06-01 00:00:00
abstract：:The purpose of this study was to examine expression and regulation of 6 multidrug efflux systems, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY, MexJK, and MexVW, in 13 non-cystic fibrosis (CF) clinical isolates of Pseudomonas aeruginosa. These isolates displayed a high level of resistance to many clinically imp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0239
更新日期：2014-07-01 00:00:00
abstract：:During growth on minimal salts - glucose media supplemented with high concentrations of Na2SO3 (10-3 and 10-2 M), Salmonella heidelberg exhibited cytological and growth responses which indicated increased cellular toxicity with increasing sulfite concentrations. The large quantities of sulfide evolved during growth at...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-217
更新日期：1979-12-01 00:00:00
abstract：:Pseudomonas aeruginosa ATCC 9027 grew on 0.5% (v/v) hexadecane as a sole carbon source in a chemically defined medium which required the addition of Fe3+ and Ca2+. There was a variable and extended lag period before an active growth rate was attained. Visible light microscopic evidence revealed that the bacteria did n...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-049
更新日期：1986-03-01 00:00:00
abstract：:In the frame of a survey of potentially endophytic N2-fixing Burkholderia associated with maize in Mexico, its country of origin, the soil of an indigenous maize field near Oaxaca was studied. Under laboratory conditions, plant seedlings of two ancient maize varieties were used as a trap to select endophyte candidates...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-023
更新日期：2002-04-01 00:00:00
abstract：:The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-239
更新日期：1976-11-01 00:00:00
abstract：:Phytopathogenic spiroplasmas can multiply in vascular plants and insects. A deeper understanding of this dual-host life could be furthered through the identification by random mutagenesis of spiroplasma genes required. The ability of the EZ::TN™
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-026
更新日期：2011-06-01 00:00:00
abstract：:alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-095
更新日期：1986-06-01 00:00:00
abstract：:The possibility of using the volatile-producing fungus Muscodor albus for biofumigation against building molds was investigated. Several species of Aspergillus and Penicillium as well as fungi belonging to nine other genera were inhibited or killed in vitro by volatiles produced by potato dextrose agar or rye grain cu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W06-124
更新日期：2007-03-01 00:00:00
abstract：:Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/m95-091
更新日期：1995-08-01 00:00:00
abstract：:Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obta...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-107
更新日期：1988-05-01 00:00:00
abstract：:The fine structure of conidia and immature resting spores of the entomogenous fungus Entomophthora apiculata is described. The two cell types contain comparable organelles and quantities of lipid, and possess a similar cell wall structure. The large quantity of stored lipid and numerous mitochondria in each cell type ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-067
更新日期：1977-04-01 00:00:00
abstract：:Porcine epidemic diarrhea virus (PEDV) causes severe infectious diseases in all ages of swine and leads to serious economic losses. Serologic tests are widely accepted and used to detect anti-PEDV antibodies that could indicate PEDV infection or vaccination. In this study, PEDV recombinant S1 protein (rS1) was express...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0240
更新日期：2019-05-01 00:00:00
abstract：:Antibiotic susceptibilities of 382 strains of Campylobacter jejuni isolated in Alberta, Canada, in 1980 and 1981 were determined. Although none were resistant to erythromycin or gentamicin, 5.4 and 22% of strains were resistant to ampicillin in 1980 and 1981, respectively. Tetracycline resistance was noted in 6.8% of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-006
更新日期：1986-01-01 00:00:00
abstract：:Several probes for measuring dissolved carbon dioxide (CO2) concentration were installed in a 68-litre fermentor and their effectiveness compared. Submerged silastic rubber tubing gave reproducible results over a wide range of operating conditions and was generally superior to all other probes evaluated. The silastic ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-007
更新日期：1976-01-01 00:00:00
abstract：:The goal of this study was to evaluate the antibiofilm and antimicrobial activities of Bacoside A, a formulation of phytochemicals from Bacopa monnieri, against Staphylococcus aureus and Pseudomonas aeruginosa, which are known to form biofilms as one of their virulence traits. The antimicrobial effects of Bacoside A w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0365
更新日期：2017-02-01 00:00:00
abstract：:Of 24 bacterial species examined for lisinopril refractive peptidyl dipeptidase activity, only 8 contained activity. Activity in Pseudomonas maltophilia was more than fourfold higher than that of any other species. Pseudomonas maltophilia may be unique among bacteria in possessing high peptidyl dipeptidase activity th...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-010
更新日期：1990-01-01 00:00:00
