Abstract：
:The poly(3-hydroxybutyrate) (PHB) depolymerase structural gene of Comamonas sp. (phaZCsp) was cloned in Escherichia coli and identified by halo formation on PHB-containing solid medium. The nucleotide sequence of a 1719 base pair MboI fragment was determined and contained one large open reading frame (ORF1, 1542 base pairs). This open reading frame encoded the precursor of the PHB depolymerase (514 amino acids; Mr, 53,095), and the deduced amino acid sequence was in agreement with the N-terminal amino acid sequence of the purified PHB depolymerase from amino acid 26 onwards. Analysis of the deduced amino acid sequence revealed a domain structure of the protein: a signal peptide that was 25 amino acids long was followed by a catalytic domain of about 300 amino acids, a fibronectin type III (Fn3) modul sequence, and a putative PHB-specific substrate-binding site. By comparison of the primary structure with that of other polyhydroxyalkanoate (PHA) depolymerases, the catalytic domain apparently contained a catalytic triad of serine, histidine, and aspartate. In addition, a conserved region resembling the oxyanion hole of lipases was present. The catalytic domain was linked to a C-terminal putative substrate-binding site by a sequence about 90 amino acids long resembling the Fn3 modul of fibronectin and other eukaryotic extracellular matrix proteins. A threonine-rich region, which was found in four of five PHA depolymerases of Pseudomonas lemoignei, was not present in the Comamonas sp. depolymerase. The similarities with and differences from other PHA depolymerases are discussed.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Jendrossek D,Backhaus M,Andermann Mdoi
10.1139/m95-183subject
Has Abstractpub_date
1995-01-01 00:00:00pages
160-9eissn
0008-4166issn
1480-3275journal_volume
41 Suppl 1pub_type
杂志文章abstract：:The microbial community plays an important role in soil nutrient cycles and energy transformations in alpine grassland. In this study, we investigated the composition of the soil microbial community collected from alpine cold swamp meadow (ASM), alpine cold meadow (AM), and alpine cold desert steppe (ADS) within the B...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2017-0585
更新日期：2018-03-01 00:00:00
abstract：:Fungal cultures were grown by a new microslide method and by the traditional slide-culture method. Sporulation results were similar by both methods. The microslide method has the advantages of increased safety to the user and ease of storage. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-204
更新日期：1978-10-01 00:00:00
abstract：:Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The induction of nitrogenase (C2H2) activity in asymbiotically cultured Rhizobium sp. 32H1 was found to be associated with morphological changes in the cells which were more pronounced than those seen in bacteroids. Polyphosphate granules were found in both bacteroids and cultured cells, but poly-beta-hydroxybutyrate ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-055
更新日期：1979-03-01 00:00:00
abstract：:Campylobacter spp. are a substantial cause of gastroenteritis worldwide. Human infection can result from ingestion of contaminated food or water from a variety of sources, including the consumption of fresh produce that is contaminated with the pathogen via the use of contaminated irrigation water. Using molecular met...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0710
更新日期：2016-05-01 00:00:00
abstract：:The rapid onset of resistance reduces the efficacy of most conventional antimicrobial drugs and is a general cause of concern for human well-being. Thus, there is great demand for a continuous supply of novel antibiotics to combat this problem. Bacteria-derived antimicrobial peptides (AMPs) have long been used as food...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/cjm-2014-0613
更新日期：2015-02-01 00:00:00
abstract：:The effect of atmospheric composition and storage temperature on growth and survival of uninjured and sublethally heat-injured Escherichia coli O157:H7, inoculated onto brain heart infusion agar containing 0.3% beef extract (BEM), was determined. BEM plates were packaged in barrier bags in air, 100% CO2, 100% N2, 20% ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w98-224
更新日期：1999-03-01 00:00:00
abstract：:Cultured alveolar and peritoneal macrophages obtained from normal and immunized rabbits were used to induce wall-defective microbial variants of Pseudomonas pseudomallei. Variants were iduced only by normal alveolar macrophages. The variants reverted to typical Pseudomonas forms either spontaneously or upon transfer i...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-303
更新日期：1975-12-01 00:00:00
abstract：:Schizophyllum commune Fr. was shown, by light, scanning, and transmission electron microscopy, to be a destructive mycoparasite on several phytopathogenic and nematode-trapping fungi. The hyphae of S. commune coiled around host hyphae and fruiting structures and penetrated them by means of either unspecialized hyphae ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-131
更新日期：1978-07-01 00:00:00
abstract：:To determine the seroprevalence of Norwalk virus and whether Norwalk virus contributed to an observed increase in illness in tap water drinkers participating in a prospective epidemiological study, sera collected during the study were examined for changes in Norwalk virus antibody titer, using a specific enzyme immuno...
journal_title：Canadian journal of microbiology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1139/m94-128
更新日期：1994-10-01 00:00:00
abstract：:Ninety-five fecal samples from Atlantic cod (Gadus morhua L.), caught along the northern Norwegian coast, were examined bacteriologically for occurrence of C. perfringens. Isolates were examined by polymerase chain reaction (PCR) for genes encoding the four lethal toxins (alpha, beta, epsilon, and iota) for classifica...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-015
更新日期：2002-04-01 00:00:00
abstract：:A comparative study of the pathogenicity of Candida albicans morphological mutant strains was made to relate chlamydospore production, germ tube formation, and proteolytic activity to candidiasis in mice. It was observed that the mycelium strains were more lethal than the yeast-like strains and that neither chlamydosp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-093
更新日期：1975-05-01 00:00:00
abstract：:The study of the relationship between MVL 51 virus, of the Gourlay group L 1, and the Mycoplasma cells of A. laidlawii during the first 35 min of infection leads to the conclusion that a "carrier state" has been established, free virus as well as infected and noninfected cells being present at the same time in the cul...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:Two potassium (K)-bearing minerals, Nanjing feldspar and Suzhou illite, were used to investigate K mobilization by the wild-type strain NBT of Bacillus edaphicus, also labeled MPs+, selected for high activity in mobilizing potassium from minerals, and by four of its UV + LiCl mutants, MPs++, MPs+1, MPs+2, and MPs-. In...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-117
更新日期：2006-01-01 00:00:00
abstract：:Ten strains of Caldariomyces fumago and related fungi were found to produce extracellular chloroperoxidase when grown on a glucose - malt extract medium. High enzyme levels and pigment production were observed for C. fumago ATCC 16373 and C. fumago CMI 89362. Removal of malt extract from the medium and the replacement...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-199
更新日期：1981-12-01 00:00:00
abstract：:The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-120
更新日期：1979-07-01 00:00:00
abstract：:Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for st...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-245
更新日期：1976-11-01 00:00:00
abstract：:Trichloroethylene (TCE) is a prevalent contaminant of groundwater that can be cometabolically degraded by indigenous microbes. Groundwater contaminated with TCE from a US Department of Energy site in Ohio was used to characterize the site-specific impact of phenol on the indigenous bacterial community for use as a pos...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-025
更新日期：2005-06-01 00:00:00
abstract：:Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-070
更新日期：1996-05-01 00:00:00
abstract：:During growth on minimal salts - glucose media supplemented with high concentrations of Na2SO3 (10-3 and 10-2 M), Salmonella heidelberg exhibited cytological and growth responses which indicated increased cellular toxicity with increasing sulfite concentrations. The large quantities of sulfide evolved during growth at...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-217
更新日期：1979-12-01 00:00:00
abstract：:Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/m95-091
更新日期：1995-08-01 00:00:00
abstract：:The catalytic subunit of many Escherichia coli redox enzymes bares a twin-arginine translocation (Tat)-dependent signal peptide in its precursor, which directs the redox enzyme complex to this Sec-independent pathway. NarG of the E. coli nitrate reductase NarGHI complex possesses a vestige twin-arginine motif at its N...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-111
更新日期：2009-02-01 00:00:00
abstract：:To expand current knowledge on the molecular aspects of alkaline phosphatase PhoX in shallow eutrophic freshwaters, we investigated the genetic diversity and abundance of the PhoX-encoding gene (phoX) in 4 ecological regions in Lake Taihu, China, following a gradient in total phosphorus concentrations ranging from hyp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0755
更新日期：2014-03-01 00:00:00
abstract：:Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDREI) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenoty...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-004
更新日期：2003-01-01 00:00:00
abstract：:Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obta...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-107
更新日期：1988-05-01 00:00:00
abstract：:Three composts (Ball, dairy, and greenhouse) were tested for the ability to suppress the development of Fusarium root and stem rot (caused by Fusarium oxysporum f. sp. radicis-cucumerinum) on greenhouse cucumber. Dairy and greenhouse composts significantly reduced disease severity (P = 0.05), while Ball compost had no...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-076
更新日期：2010-11-01 00:00:00
abstract：:Environmentally compatible control measures are needed for suppression of Phytophthora capsici on pepper. Twenty-three isolates of Trichoderma were screened for suppression of a mixture of 4 genetically distinct isolates of this pathogen on bell pepper (Capsicum anuum) in greenhouse pot assays. Of these 23 isolates, G...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-071
更新日期：2010-10-01 00:00:00
abstract：:The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-239
更新日期：1976-11-01 00:00:00
abstract：:Total community DNA from 29 noncontaminated soils and soils impacted by petroleum hydrocarbons and chloro-organics from Antarctica and Brazil were screened for the presence of nine catabolic genes, encoding alkane monooxygenase or aromatic dioxygenases, from known bacterial biodegradation pathways. Specific primers an...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-008
更新日期：2004-05-01 00:00:00
abstract：:Seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (IA and IB), two on day 4 (IVA and IVB), one on day 8 (VIIIA), and two on day 9 (IXA and IXB). The lambs were reared in these isolators until ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-064
更新日期：1989-03-01 00:00:00
