Abstract：
:Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a sensitive, rapid, and inexpensive screening test to detect FP in agricultural commodities is necessary to protect human health. FP-hemiglutarate conjugated to modified bovine serum albumin was synthesized, characterized, and used as an antigen for raising polyclonal antibodies by immunizing rabbits. Indirect and competitive ELISA and immunoblotting analyses were performed to determine antibody specificity towards the mycotoxin. The determination of 10 micrograms of free FP/mL was achieved using antibodies purified by means of affinity chromatography on a FP-lysine-Sepharose column. This unsatisfactory detection limit is due to high background values; thus, this method is not competitive with traditional UV-HPLC methods.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Monti SM,Fogliano V,Randazzo G,Peluso G,Logrieco A,Ritieni Asubject
Has Abstractpub_date
1999-01-01 00:00:00pages
45-50issue
1eissn
0008-4166issn
1480-3275journal_volume
45pub_type
杂志文章abstract：:Corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. Corynebacterium glutamicum his genes are located and transcribed in two unlinked loci, hisEG and hisDCB-orf1-orf2-hisHA-impA-hisFI. The latter his operon starts the transcription at the C residue localized...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-115
更新日期：2010-02-01 00:00:00
abstract：:The effect of the transition metal compound trans-[Rh(4-ethylpyridine)4Cl2]Cl x 2H2O on the syntheses of DNA, RNA, and protein has been investigated for an auxotrophic bacterial strain, Escherichia coli JS-1, incapable of thymidine, uridine, and histidine syntheses. At low concentration (7.4 x 10(-6) M), this rhodium ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-111
更新日期：1980-05-01 00:00:00
abstract：:An alginate lyase gene of a newly isolated Pseudomonas sp. strain KS-408 was cloned by using PCR with the specific primers designed from homologous nucleotide sequences. A partial protein sequence of KS-408 alginate lyase was homology-modeled on the basis of the crystal structure of A1-III alginate lyase from Sphingom...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-106
更新日期：2011-12-01 00:00:00
abstract：:Farnesol, a quorum sensing (QS) signal, is produced by Candida albicans during high density growth and has been found to inhibit morphogenesis. This QS auto-inducing signal was discovered to increase amino acid incorporation by C. albicans when concentrations of farnesol increased to 10 microg/mL in yeast nitrogen bro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-050
更新日期：2005-08-01 00:00:00
abstract：:Andosols comprise one of the most important soil groups for agricultural activities in Japan because they cover about 46.5% of arable upland fields. In this soil group, available phosphorus (P) is accumulated by application of excessive fertilizer, but little is known about the influence of increasing P availability o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0612
更新日期：2013-06-01 00:00:00
abstract：:The effects of various hydrocarbon substrates, and a chemical surfactant capable of enhancing crude-oil biodegradation, on the community structure of a mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% were identified at the genus level or better by phospholipid fatty acid...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:Legionella pneumophila grows in amoebae and has achieved the ability to grow at various temperatures, although the mechanisms controlling this ability remain poorly understood. The Icm/Dot type IVB secretion system is composed of more than 25 proteins and is known to be essential for intracellular growth. The role of...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-011
更新日期：2012-04-01 00:00:00
abstract：:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent micr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-063
更新日期：1979-03-01 00:00:00
abstract：:To screen, isolate, and characterize bacterial populations producing microcin J25, we report here two rapid, reliable, and sensitive methods, using polymerase chain reaction and colony blot hybridization with a digoxigenin-labelled probe. A sample of 26 Escherichia coli strains isolated from poultry intestinal content...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:The study of diaminopimelic acid (DAP) incorporation and turnover during growth recovery in chloramphenicol-treated (CMP-treated) Bacillus megaterium cells showed that two kinds of turnover occurred. A low acid-soluble turnover appeared as soon as growth resumed in bacteria labeled before the CMP treatment and at the ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-051
更新日期：1980-03-01 00:00:00
abstract：:Boron (B) is toxic to living cells at levels above a certain threshold. We isolated several B-tolerant bacterial strains from soil samples and studied them for possible mechanisms of B tolerance. 16S rRNA gene sequencing and comparative phylogenetic analysis demonstrated that the isolates belong to the following 6 gen...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-106
更新日期：2010-01-01 00:00:00
abstract：:No clear data are available on how flavonoids from different chemical groups affect root colonization by arbuscular mycorrhizal fungi (AMF) and whether flavonoids affecting the presymbiotic growth of AMF also affect root colonization by AMF. In the present work, we compared the effect of flavones (chrysin and luteolin...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-036
更新日期：2007-06-01 00:00:00
abstract：:An Escherichia coli K-12 biofilm was grown at a dilution rate of 0.028 h(-1) for 48 h in a glucose-limited chemostat coupled to a modified Robbins' device to determine its susceptibility to infection by bacteriophage T4. Bacteriophage T4 at a multiplicity of infection (MOI) of 10 caused a log reduction in biofilm dens...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and th...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-236
更新日期：1978-12-01 00:00:00
abstract：:Examination of immune complexes between cells of Streptococcus mutans and homologous antiserum by the techniques of thin-sectioning and freeze-etching revealed that the cells were embedded within and extensive matrix 80-90 nm thick with defined boundaries. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-129
更新日期：1976-06-01 00:00:00
abstract：:A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylind...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-151
更新日期：1979-09-01 00:00:00
abstract：:Chloroquine increases the inhibition of cultured lymphocytes by high concentrations of phytohemagglutinin (PHA) or concanavalin A (Con A). The inhibition is also increased by complement. Thus chloroquine and complement have similar effects. Time-course studies show that chloroquine increases the rate of onset of the c...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-231
更新日期：1975-10-01 00:00:00
abstract：:The 16S-23S rDNA spacer of the type strain (ATCC 35879) of Xylella fastidiosa was amplified by PCR, cloned, and sequenced. The spacer sequence (455 bp) contains two tRNA (tRNA(ala) and tRNA(ile)) genes. Identical tRNA genes were also found in the 16S-23S spacer sequences of all the 51 strains of X. fastidiosa retrieve...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-114
更新日期：2000-12-01 00:00:00
abstract：:Caulobacters are biofilm-forming members of the natural flora of soil and aquatic environments, which exhibit several characteristics that make them attractive for development of high surface area microbial bioreactors or biosensors. Although caulobacters are well characterized genetically, little is known about their...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:Several probes for measuring dissolved carbon dioxide (CO2) concentration were installed in a 68-litre fermentor and their effectiveness compared. Submerged silastic rubber tubing gave reproducible results over a wide range of operating conditions and was generally superior to all other probes evaluated. The silastic ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-007
更新日期：1976-01-01 00:00:00
abstract：:DNA hybridization studies were conducted to determine the taxonomic status of the aquatic group of Metschnikowia species and their varieties. Among the DNAs of the four varieties of Metschnikowia bicuspidata, that of Metschnikowia bicuspidata var. australis showed 37 to 51% relative binding with the DNAs of Metschniko...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-170
更新日期：1985-10-01 00:00:00
abstract：:The effects of selected culture conditions on production of Shiga-like toxin-II variant by an edema disease strain of Escherichia coli (412) and E. coli TB1 (pCG6) containing the cloned genes for Shiga-like toxin-II variant were examined. Incubation time, culture media, incubation temperature, starting pH of the cultu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-100
更新日期：1989-06-01 00:00:00
abstract：:Immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the Paramyxoviridae family: the two external membrane glycoproteins H (90 000) and Fo (F1, 49 000; F2, 20 000; disulfide linked), the internal membrane protein M (34 000), t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-004
更新日期：1986-01-01 00:00:00
abstract：:A quantitative and qualitative survey of the indigenous Endogone population in wheat field soil and vesicular-arbuscular mycorrhiza in wheat roots as influenced by season and by the stage of development of the wheat plant was made. The number of Endogone spores in wheat field soil remained relatively unchanged in win...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-151
更新日期：1975-07-01 00:00:00
abstract：:Dark spots disease (DSD) is a relatively new coral disease that has become one of the most prevalent afflictions in the Caribbean Sea. To partially characterize bacterial communities associated with DSD, carbon utilization patterns of bacterial strains isolated from the surface mucopolysaccharide layers of healthy and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-002
更新日期：2007-04-01 00:00:00
abstract：:We present preliminary data on the role of antibiotics in intraabdominal sepsis using a new, clinically relevant animal model. Peritoneal cavity infection was induced by ligation and perforation of the cecum in adult rats. Surviving rats were randomized to receive either saline or cefoxitin at the time of cecal excisi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-059
更新日期：1988-03-01 00:00:00
abstract：:The prevalence of Coxiella burnetti infection (Q fever) was determined among Nova Scotia (N.S.) and Prince Edward Island (P.E.I.) blood donors by using the complement fixation and microimmunofluorescence (IF) test. The complement fixation and IF antibody tests measured antibody prevalence for the phase II or phase I a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-021
更新日期：1984-01-01 00:00:00
abstract：:Staphylococcus aureus was grown in a rich peptone medium which became alkaline with continued incubation. Cells were grown at 37 degrees C and in the same medium containing 1 M NaCl at 46 degrees C, a temperature at which this organism can grow only when protected by NaCl. Cells of these cultures are hereafter called ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-049
更新日期：1983-03-01 00:00:00
abstract：:The bacterial flora of an experimental plant that processes liquid swine manure by an aerated compartmented (multistage) lagoon system has been studied. The total flora is characterized by a larger number of oligotrophic bacteria than eutrophic ones. Each compartment displays a specific flora, different from the flora...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-154
更新日期：1997-11-01 00:00:00
abstract：:Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-148
更新日期：1976-07-01 00:00:00
