Abstract:
:A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylinder of agar during sterilization, a minimum of head space, and use of a reducing agent to absorb oxygen introduced during the inoculation procedure. For several species of Clostridium, Bacteroides fragilis, Fusobacterium necrophorum, Veillonella alcalescens, and Pectinatus cerevisiiphilus, colony counts of cultures in the Lee tubes were comparable with those obtained in pour plates incubated in a BBL GasPak system and in anaerobic roll tubes.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Ogg JE,Lee SY,Ogg BJdoi
10.1139/m79-151subject
Has Abstractpub_date
1979-09-01 00:00:00pages
987-90issue
9eissn
0008-4166issn
1480-3275journal_volume
25pub_type
杂志文章abstract::Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC st...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-063
更新日期:2012-07-01 00:00:00
abstract::The ultraviolet germicidal irradiation (UVGI) dose necessary to inactivate fungal spores on an agar surface and the efficacy of UVGI were determined for cultures of Stachybotrys chartarum (ATCC 208877). This study employed a UVGI testing unit consisting of four chambers with a 9-W, Phillips, low pressure, mercury UVGI...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-061
更新日期:2005-09-01 00:00:00
abstract::Fluorescent antibody was prepared against a temperate-soil isolate of Beijerinckia obtained from a rhizosphere of rice growing in Camargue (France). The antibody did not cross-react with any of 6 species of Azotobacter, 4 species of Beijerinckia, or 44 unidentified soil bacteria isolated from a spectrum of rhizosphere...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-023
更新日期:1977-02-01 00:00:00
abstract::Brachiaria, predominantly an African genus, contains species, such as B. brizantha, an apomictic C4 grass, that are commercially important forage grasses in tropical America, where they now cover about 55 million hectares. From B. brizantha accession CIAT 6780, we isolated an endophytic fungus that may be economically...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-127
更新日期:2001-01-01 00:00:00
abstract::Uptake of amino acids by Bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. A high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. All amino acids except proline we...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-180
更新日期:1979-10-01 00:00:00
abstract::A tryptophan auxotroph of aerobically grown Rodospirillum rubrum was isolated after mutagenesis and replica plating. The mutant grows on tryptophan or indole, accumulates indole glycerol phosphate, lacks the capacity to convert indole glycerol phosphate to indole glycerol phosphate to indole, and finally is defective ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-055
更新日期:1978-03-01 00:00:00
abstract::Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the results were interpreted ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W08-098
更新日期:2008-12-01 00:00:00
abstract::The purpose of this study was to examine expression and regulation of 6 multidrug efflux systems, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY, MexJK, and MexVW, in 13 non-cystic fibrosis (CF) clinical isolates of Pseudomonas aeruginosa. These isolates displayed a high level of resistance to many clinically imp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0239
更新日期:2014-07-01 00:00:00
abstract::Haemophilus paragallinarum secretes metalloproteases into different culture media lacking serum. Secreted proteins, concentrated by precipitation with 70% ammonium sulphate ((NH(4))(2)SO(4)) or methanol, displayed proteolytic activity at >100 kDa molecular mass in 10% polyacrylamide gels co-polymerized with porcine ge...
journal_title:Canadian journal of microbiology
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doi:10.1139/w05-067
更新日期:2005-10-01 00:00:00
abstract::The results of sucrose-density-gradient centrifugation of a cell-free particulate enzyme system from Acetobacter xylinum which was incubated with uridine diphosphoglucose indicate that there is a polymeric intermediate in the biosynthesis of cellulose. This intermediate has the properties of an oligomer of glucose, is...
journal_title:Canadian journal of microbiology
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doi:10.1139/m75-018
更新日期:1975-02-01 00:00:00
abstract::From electron-microscopical observations, a decreased metabolic activity in 3-day-old Candida albicans chlamydospores was suggested, and progressive deterioration in chlamydospores aged 2-8 months was shown. Oxygen utilization by chlamydospore-pseudomycelium (CSP) preparations was less than that by yeast, while 3-day-...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-111
更新日期:1979-06-01 00:00:00
abstract::This study, the first of arbovirus activity in Prince Edward Island, has shown antibodies to the snowshoe hare strain of the California encephalitis group virus to be present in sera of wild and domestic animals; 15.35% of snowshoe hare, 20% of equine. and 2.5% of bovine sera were positive for hemagglutination inhibit...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-188
更新日期:1981-11-01 00:00:00
abstract::Staphylococcus aureus isolated from the cervix of an infertile woman was found to cause complete immobilization of human spermatozoa in vitro. Only the cell culture and cell-free supernatant showed immobilization activity, indicating that the sperm immobilization factor might be released extracellularly by the organis...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-032
更新日期:2009-07-01 00:00:00
abstract::A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-106
更新日期:1983-06-01 00:00:00
abstract::Clinical isolates of Staphylococcus aureus were found to exhibit strain-specific heterogeneity to the growth-enhancing effects of human urokinase (UK), a proteinase with plasminogen activator activity. Nine out of fourteen (64%) methicillin-sensitive strains of S. aureus were responsive to UK in "in vitro" cultures. I...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-131
更新日期:1996-10-01 00:00:00
abstract::Investigations of microbial diversity in Antarctic are important to begin to understand ecosystem functioning and decomposition processes. This study documents fungi at 9 historic sites on the Antarctic Peninsula collected from wooden structures, other organic materials, and soils during a joint National Science Found...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W08-120
更新日期:2009-01-01 00:00:00
abstract::The effect of mechanical wounding or foliar diseases caused by Sclerotinia homoeocarpa or Rhizoctonia solani on the epiphytic yeast communities on creeping bentgrass and tall fescue were determined by leaf washing and dilution plating. Total yeast communities on healthy bentgrass and tall fescue leaves ranged from 7.9...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-073
更新日期:2004-10-01 00:00:00
abstract::DNA-degrading activity from anaerobic samples of bovine ruminal fluid, primary anaerobic digestor wastewater, freshwater sediments, and marine sediments was observed in the presence of 5 mM EDTA. Nuclease activity experiments involved exposing salmon chromosomal DNA to the environmental samples in 50 mM pH 7.2 buffer,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obta...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-107
更新日期:1988-05-01 00:00:00
abstract::No clear data are available on how flavonoids from different chemical groups affect root colonization by arbuscular mycorrhizal fungi (AMF) and whether flavonoids affecting the presymbiotic growth of AMF also affect root colonization by AMF. In the present work, we compared the effect of flavones (chrysin and luteolin...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-036
更新日期:2007-06-01 00:00:00
abstract::An alginate lyase gene of a newly isolated Pseudomonas sp. strain KS-408 was cloned by using PCR with the specific primers designed from homologous nucleotide sequences. A partial protein sequence of KS-408 alginate lyase was homology-modeled on the basis of the crystal structure of A1-III alginate lyase from Sphingom...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-106
更新日期:2011-12-01 00:00:00
abstract::Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and th...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-236
更新日期:1978-12-01 00:00:00
abstract::BaeR, a response regulator protein, takes part in multidrug efflux, bacterial virulence activity, and other biological functions. Recently, BaeR was shown to induce inflammatory responses by activating the mitogen-activated protein kinases (MAPKs). In this study, we investigated additional pathways used by BaeR to ind...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00
abstract::The initial cause of the cessation of patulin biosynthesis in submerged cultures of Penicillium urticae was sought. The extrinsic limitations to patulin biosynthesis in fermentor cultures were successfully removed and kinetic profiles of the metabolites of the pathway and of three pathway enzymes were determined over ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-032
更新日期:1981-02-01 00:00:00
abstract::Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-153
更新日期:1978-08-01 00:00:00
abstract::A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-059
更新日期:1997-05-01 00:00:00
abstract::Studies of the molecular biology of human papillomavirus type 16 have been limited by the lack of a tissue culture system that is fully permissive for virus replication; as a result, high-titre stocks of infectious virus are not readily available. Therefore, studies of viral gene expression have relied on analysis of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-016
更新日期:1993-01-01 00:00:00
abstract::Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-164
更新日期:1980-08-01 00:00:00
abstract::A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-162
更新日期:1997-12-01 00:00:00
abstract::Chloroquine increases the inhibition of cultured lymphocytes by high concentrations of phytohemagglutinin (PHA) or concanavalin A (Con A). The inhibition is also increased by complement. Thus chloroquine and complement have similar effects. Time-course studies show that chloroquine increases the rate of onset of the c...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-231
更新日期:1975-10-01 00:00:00