A modified tube method for the cultivation and enumeration of anaerobic bacteria.

Abstract:

:A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylinder of agar during sterilization, a minimum of head space, and use of a reducing agent to absorb oxygen introduced during the inoculation procedure. For several species of Clostridium, Bacteroides fragilis, Fusobacterium necrophorum, Veillonella alcalescens, and Pectinatus cerevisiiphilus, colony counts of cultures in the Lee tubes were comparable with those obtained in pour plates incubated in a BBL GasPak system and in anaerobic roll tubes.

journal_name

Can J Microbiol

authors

Ogg JE,Lee SY,Ogg BJ

doi

10.1139/m79-151

subject

Has Abstract

pub_date

1979-09-01 00:00:00

pages

987-90

issue

9

eissn

0008-4166

issn

1480-3275

journal_volume

25

pub_type

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