Abstract:
:Ribosomal preparations from Neisseria gonorrhoeae types 1 and 4 were examined for their in vitro stimulation of mouse splenocytes to determine the ribosomal moiety or contaminant responsible for the immunoproliferative activity. In immunodiffusion tests with homologous rabbit antiserum, crude 70S ribosomes formed four precipitin bands while the purified 30S and 50S subunits showed one major line. The same antiserum reacted with lysed N. gonorrhoeae and Neisseria meningitidis A cells but no precipitation occurred with Escherichia coli cells purified N. gonorrhoeae lipopolysaccharide (LPS). No membrane or LPS contaminant was detected in the purified 30S and 50S preparations. All the ribosomal preparations from virulent and non-virulent N. gonorrhoeae consistently stimulated the murine splenocytes. The mitogenic activity of the 30S and 50S ribosomal preparation was destroyed by treatment with trypsin but only slightly decreased by ribonuclease. It is suggested that the lymphoproliferative response elicited by gonococcal ribosomes is triggered by the protein moiety of the 30S or 50S subunits.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Brodeur BRdoi
10.1139/m78-094subject
Has Abstractpub_date
1978-05-01 00:00:00pages
579-85issue
5eissn
0008-4166issn
1480-3275journal_volume
24pub_type
杂志文章abstract::A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-059
更新日期:1997-05-01 00:00:00
abstract::To determine the ability of environmental bacteria to metabolize the frequently prescribed fluoroquinolone drug ciprofloxacin, eight Mycobacterium spp. cultures were grown for 4 days in a medium containing sorbitol and yeast extract with 100 mg x L(-1) ciprofloxacin. After the cultures had been centrifuged and the sup...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-101
更新日期:2007-01-01 00:00:00
abstract::Considering the medical, biotechnological, and economical importance of actinobacteria, there is a continuous need to improve the tools for genetic engineering of a broad range of these microorganisms. Intergeneric conjugation has proven to be a valuable yet imperfect tool for this purpose. The natural resistance of m...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0041
更新日期:2015-08-01 00:00:00
abstract::The addition of various concentrations of monensin (1,5, and 10 micrograms/mL) to the culture medium inhibits the fungal growth and perturbs exopolysaccharides secretion, provoking a decrease of production in Botrytis cinerea and an increase in Sclerotium rolfsii. The ionophore induces also modifications in both polym...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-124
更新日期:1996-09-01 00:00:00
abstract::Helicobacter pylori exists in 2 distinct morphological states, helicoid and coccoid. Both have been observed in in vitro culture and in gastric biopsies. We visualized H. pylori during AGS cell infections using immunofluorescence microscopy. Anti-H. pylori mouse serum as well as human serum from H. pylori-positive pat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-042
更新日期:2008-07-01 00:00:00
abstract::Plant-microbial interactions have been well studied because of the ecological importance of such relationships in aquatic systems. However, general knowledge regarding the composition of these biofilm communities is still evolving, partly as a result of several confounding factors that are attributable to plant host p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-053
更新日期:2012-06-01 00:00:00
abstract::Fluorescent antibody was prepared against a temperate-soil isolate of Beijerinckia obtained from a rhizosphere of rice growing in Camargue (France). The antibody did not cross-react with any of 6 species of Azotobacter, 4 species of Beijerinckia, or 44 unidentified soil bacteria isolated from a spectrum of rhizosphere...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-023
更新日期:1977-02-01 00:00:00
abstract::The repair of DNA damage caused by ultraviolet radiation (UVR) is well understood in both lower and higher organisms. Genetic studies carried out at optimum temperature for growth, 37 °C in Escherichia coli, have revealed the major pathways of DNA repair. We show that E. coli cells grown at 20 °C are more sensitive to...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0874
更新日期:2014-05-01 00:00:00
abstract::In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-188
更新日期:1979-10-01 00:00:00
abstract::The phyllosphere, which supports a large number of microorganisms, represents the interface between the aboveground parts of plants and air. In this study, four nifH clone libraries were constructed from the phyllosphere of Pyrus serotina (L), Vitis vinifera (P), Prunus armeniaca (X), and Prunus avium (Y). Clones rela...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0588
更新日期:2019-09-01 00:00:00
abstract::Seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (IA and IB), two on day 4 (IVA and IVB), one on day 8 (VIIIA), and two on day 9 (IXA and IXB). The lambs were reared in these isolators until ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-064
更新日期:1989-03-01 00:00:00
abstract::Legionella pneumophila grows in amoebae and has achieved the ability to grow at various temperatures, although the mechanisms controlling this ability remain poorly understood. The Icm/Dot type IVB secretion system is composed of more than 25 proteins and is known to be essential for intracellular growth. The role of...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-011
更新日期:2012-04-01 00:00:00
abstract::Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteri...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0343
更新日期:2020-10-01 00:00:00
abstract::The in situ degradation of the two nitramine explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), was evaluated using a mixture of RDX and HMX, incubated anaerobically at 10 degrees C with marine sediment from a previous military dumping site of unexplod...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-112
更新日期:2004-02-01 00:00:00
abstract::Cellulomonas fimi genomic DNA was digested with HpaI, MunI, HindIII, and NsiI, producing fragments ranging in size from 20 to 1400 kbp that were resolved by pulsed field gel electrophoresis. Genetic and physical linkages were determined by Southern blotting and were used to construct a genome map. Cellulomonas fimi ha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::The deliberate application of the methodology of contemporary molecular genetics to problems in bacterial systematics has led to a broad new understanding of the evolutionary history of both prokaryotes and eukaryotes. In this review, I discuss some of the major conclusions of this endeavour and try to predict future ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/m88-093
更新日期:1988-04-01 00:00:00
abstract::Enterococci, formerly confounded with faecal streptococci, are recognized since the beginning of the century as being faecal in origin and are generally searched for in waste waters and food products; their detection may in fact indicate the presence of enteropathogenic organisms. Although nearly ubiquitous, their pre...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::Incubation of 4'-chloro-4-biphenylol with a mushroom tyrosinase preparation gave the catechol, 4'-chloro-3,4-biphenyldiol as the sole in vitro metabolite. This compound was identical with the major rat urinary metabolite of 4'-chloro-4-biphenylol and thus confirms the structure assigned to the metabolite. This result ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-014
更新日期:1976-01-01 00:00:00
abstract::Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-227
更新日期:1976-10-01 00:00:00
abstract::Repetitive element-polymerase chain reaction (REP-PCR) DNA fingerprinting and library-based microbial source tracking (MST) methods were utilized to investigate the potential sources of Escherichia coli pollution in recreational waters of southeastern Lake Huron. In addition to traditional sources such as humans, agri...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-123
更新日期:2009-03-01 00:00:00
abstract::Twenty-six strains of group JK corynebacteria had the same colonial morphology and biological reactions as the biotypes of the biovars of Corynebacterium genitalium and C. pseudogenitalium. Therefore, group JK corynebacteria can be assigned to the biovars of C. genitalium or C. pseudogenitalium. Although the strains d...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-164
更新日期:1984-08-01 00:00:00
abstract::A quantitative and qualitative survey of the indigenous Endogone population in wheat field soil and vesicular-arbuscular mycorrhiza in wheat roots as influenced by season and by the stage of development of the wheat plant was made. The number of Endogone spores in wheat field soil remained relatively unchanged in win...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-151
更新日期:1975-07-01 00:00:00
abstract::The ruminal microbiota plays major roles in feed digestion. The composition and fermentation of the bacterial communities in 3 important ruminant species have been studied previously. Here, we extended this research to the effect of concentrate-to-forage ratios on ruminal bacterial communities in Tarim red deer (Cervu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0046
更新日期:2018-07-01 00:00:00
abstract::Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-148
更新日期:1976-07-01 00:00:00
abstract::A high molecular component of a saline extract derived from Listeria monocytogenes contained amino acids, carbohydrates, and phosphorus. The same fraction was capable of promoting both the in vitro mitogenic and adjuvant activities and the in vivo immunosuppressive activity displayed by the crude extract. The material...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-204
更新日期:1982-12-01 00:00:00
abstract::A Gram-negative aerobic bacterium, designated as JN8, was isolated from activated sludge and soil in a pesticides factory in China. It was found that JN8 had a high capacity for degrading a broad range of type II pyrethroids and utilizing these pyrethroids as the sole carbon source for cell growth. The degradation rat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0104
更新日期:2014-08-01 00:00:00
abstract::Nodulation of soybeans by indigenous and inoculum strains of Bradyrhizobium japonicum was studied in field experiments in Wisconsin from 1983 to 86. Aqueous suspensions of bacteria were applied to seeds at the time of planting at levels of 7 × 10(7)-10(10) bacteria per 2.5-cm row. The predominant indigenous serogroup ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-136
更新日期:1990-11-01 00:00:00
abstract::Pseudomonas aeruginosa establishes life-long chronic infections in the cystic fibrosis (CF) lung by utilizing various adaptation strategies. Some of these strategies include altering metabolic pathways to utilize readily available nutrients present in the host environment. The airway sputum contains various host-deriv...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0485
更新日期:2014-12-01 00:00:00
abstract::Our purpose was to study bacterial antagonism between a limited number of strictly anaerobic strains and Clostridium perfringens in the intestinal tract of gnotobiotic rodents. Gnotobiotic mice harboring a Bacteroides thetaiotaomicron, a Fusobacterium necrogenes, and a Clostridium sp. strain were protected against pat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-039
更新日期:1987-03-01 00:00:00
abstract::The survival of and interactions between a Pseudomonas putida strain labelled with a red fluorescent protein gene (WT-rfp) and its green fluorescent protein gene-labelled rpoS(-) mutant (KO-gfp) were examined. The generation times of the planktonic WT-rfp and KO-gfp in trypticase soy broth were not significantly diffe...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-081
更新日期:2009-10-01 00:00:00