Abstract:
:We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library sequence analysis indicated similar patterns of midgut microbial community structure and diversity: specific bacterial types existed in both populations, and a more diverse microbial community was observed in caterpillars obtained from the field. The laboratory population harbored a rather simple gut microflora consisting mostly of phylotypes belonging to Enterococcus (84%). For the field population, phylotypes belonging to Enterococcus (28%) and Lactococcus (11%), as well as Flavobacterium (10%), Acinetobacter (19%), and Stenotrophomonas (10%) were dominant members. These results provided the first comprehensive description of the microbial diversity of the midgut of the important pest cotton bollworm and suggested that the environment and food supply might influence the diversity of the gut bacterial community.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Xiang H,Wei GF,Jia S,Huang J,Miao XX,Zhou Z,Zhao LP,Huang YPdoi
10.1139/w06-064subject
Has Abstractpub_date
2006-11-01 00:00:00pages
1085-92issue
11eissn
0008-4166issn
1480-3275pii
w06-064journal_volume
52pub_type
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