The effect of temperature and other growth conditions on the fatty acid composition of Escherichia coli.

abstract：:The microbial community plays an important role in soil nutrient cycles and energy transformations in alpine grassland. In this study, we investigated the composition of the soil microbial community collected from alpine cold swamp meadow (ASM), alpine cold meadow (AM), and alpine cold desert steppe (ADS) within the B...

journal_title：Canadian journal of microbiology

authors： Shao K,Gao G

更新日期：2018-03-01 00:00:00

abstract：:Several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. This pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochro...

journal_title：Canadian journal of microbiology

authors： Jacobs NJ,O'Hara J,Gray CT

更新日期：1983-09-01 00:00:00

abstract：:Members of the genus Bacillus and related spore-forming genera are ubiquitous. However, Bacillus-like species isolated from marine sediments have attracted less interest than their terrestrial relatives. Here, we investigated the diversity of Bacillus-like bacterial communities in the sediments of the Bamenwan mangrov...

journal_title：Canadian journal of microbiology

authors： Liu M,Cui Y,Chen Y,Lin X,Huang H,Bao S

更新日期：2017-03-01 00:00:00

abstract：:The PII protein is apparently involved in the control of NifA activity in Herbaspirillum seropedicae. To evaluate the probable role of PII in signal transduction, uridylylation assays were conducted with purified H. seropedicae PII and Escherichia coli GlnD, or a cell-free extract of H. seropedicae as sources of uridy...

journal_title：Canadian journal of microbiology

authors： Benelli EM,Buck M,de Souza EM,Yates MG,Pedrosa FO

更新日期：2001-04-01 00:00:00

abstract：:A nystatin-resistant mutant of Aspergillus niger is described that has quantitative changes in phospholipid composition compared with the wild type. The proportion of linoleic acid in total phospholipids was elevated in the mutant from 38 to 56% and the proportion of oleic acid was lowered from 28 to 12%. Amino acid t...

journal_title：Canadian journal of microbiology

authors： Mazumder C,Basu J,Kundu M,Chakrabarti P

更新日期：1990-06-01 00:00:00

abstract：:Lysis of mid-log phase cells of the Gram-positive bacterium, Corynebacterium sepedonicum, by a combination of lysozyme treatment and incubation with alkaline sodium dodecyl sulfate at 56 degrees C led to the recovery of a single plasmid. The plasmid was purified in CsCl density gradients, and its molecular weight esti...

journal_title：Canadian journal of microbiology

authors： Clark MC,Lawrence CH

更新日期：1986-08-01 00:00:00

abstract：:The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation pat...

journal_title：Canadian journal of microbiology

authors： Kudo H,Jakober KD,Phillippe RC,Cheng KJ,Barr DJ,Costerton JW

更新日期：1990-07-01 00:00:00

abstract：:A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...

journal_title：Canadian journal of microbiology

authors： King EJ,Brown MF

更新日期：1983-06-01 00:00:00

abstract：:Using a gene fusion approach, hybrid proteins were created by linking alkaline phosphatase (PhoA) or a cellulase reporter (delta CenA) to four large N-terminal portions of the Caulobacter crescentus surface (S)-layer protein (RsaA; 1026 amino acids). Three of the sites (amino acids 189, 220, 315) were selected on the ...

journal_title：Canadian journal of microbiology

authors： Bingle WH,Smit J

更新日期：1994-09-01 00:00:00

abstract：:Yersinia pestis is a bacterium that is transmitted between fleas, which have a body temperature of 26 °C, and mammalian hosts, which have a body temperature of 37 °C. To adapt to the temperature shift, phenotype variations, including virulence, occur. In this study, an antigen microarray including 218 proteins of Y. p...

journal_title：Canadian journal of microbiology

authors： Li B,Tan Y,Guo J,Cui B,Wang Z,Wang H,Zhou L,Guo Z,Zhu Z,Du Z,Yang R

更新日期：2011-04-01 00:00:00

abstract：:The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intra...

journal_title：Canadian journal of microbiology

authors： Diena BB,Ashton FE,Ryan A,Wallace R

更新日期：1978-02-01 00:00:00

abstract：:To determine the seroprevalence of Norwalk virus and whether Norwalk virus contributed to an observed increase in illness in tap water drinkers participating in a prospective epidemiological study, sera collected during the study were examined for changes in Norwalk virus antibody titer, using a specific enzyme immuno...

journal_title：Canadian journal of microbiology

authors： Payment P,Franco E,Fout GS

更新日期：1994-10-01 00:00:00

abstract：:The lytic effect of lysozyme on Streptococcus faecalis ATCC 9790 was studied by spectrophotometry and electron microscopy and it was found to be highly dependent on the ionic strength of the suspending media and on the ratio lysozyme to bacterial cell mass. When 7.2 X 10(8) bacteria/mL are exposed to 0.4 mg/mL of lyso...

journal_title：Canadian journal of microbiology

authors： Carvalho ME,Gonçalves MH,Silva MT

更新日期：1984-07-01 00:00:00

abstract：:The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cyto...

journal_title：Canadian journal of microbiology

authors： Pereira Md,Byrne BA,Nguyen TB,Lewis DJ,Atwill ER

更新日期：2013-06-01 00:00:00

abstract：:The 16S-23S rDNA spacer of the type strain (ATCC 35879) of Xylella fastidiosa was amplified by PCR, cloned, and sequenced. The spacer sequence (455 bp) contains two tRNA (tRNA(ala) and tRNA(ile)) genes. Identical tRNA genes were also found in the 16S-23S spacer sequences of all the 51 strains of X. fastidiosa retrieve...

journal_title：Canadian journal of microbiology

authors： Chen J,Banks D,Jarret RL,Jones JB

更新日期：2000-12-01 00:00:00

abstract：:The acetylene-reduction assay was used for in situ and laboratory assessment of biological nitrogen fixation in the acidic, waterlogged, muskeg ecosystem of the southern James Bay area, in the region of Moosonee, Ontario. In situ assays and subsequent laboratory experimentation revealed that nitrogenase activity was p...

journal_title：Canadian journal of microbiology

authors： Blasco JA,Jordan DC

更新日期：1976-07-01 00:00:00

abstract：:Arsenic hyperaccumulator fern Pteris vittata L. produces large amounts of root exudates that are hypothesized to solubilize arsenic and maintain a unique rhizosphere microbial community. Total heterotrophic counts on rich or defined media supplemented with up to 400 mmol/L of arsenate showed a diverse arsenate-resista...

journal_title：Canadian journal of microbiology

authors： Huang A,Teplitski M,Rathinasabapathi B,Ma L

更新日期：2010-03-01 00:00:00

abstract：:Ten bacterial strains were isolated by enrichment culture, using as carbon sources either aliphatics or an aromatic-polar mixture. Oxygen uptake rate was used as a criterion to determine culture transfer timing at each enrichment stage. Biodegradation of aliphatics (10,000 mg L(-1)) and an aromatic-polar mixture (5000...

journal_title：Canadian journal of microbiology

authors： Díaz-Ramírez IJ,Ramírez-Saad H,Gutiérrez-Rojas M,Favela-Torres E

更新日期：2003-12-01 00:00:00

abstract：:The study investigated the presence of Borrelia-specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG pos...

journal_title：Canadian journal of microbiology

authors： Lencáková D,Stefancíková A,Ivanová R,Petko B

更新日期：2007-12-01 00:00:00

abstract：:A soluble cytochrome P450 (P450EP1A) induced by 2-ethoxyphenol was purified to apparent homogeneity from Corynebacterium sp. strain EP1. The P450EP1A showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of about 45 kDa. The CO-reduced difference spectra of P450EP1A ...

journal_title：Canadian journal of microbiology

authors： Kawahara N,Ikatsu H,Kawata H,Miyoshi S,Tomochika K,Sinoda S

更新日期：1999-10-01 00:00:00

abstract：:Effects of temperature and anaerobic conditions on the replication of two bacteriophages, nt-1 and nt-6, of the estuarine bacterium Vibrio natriegens were studied. Reduction in temperature resulted in longer latent periods and reduced burst sizes for both phages. Replication under anaerobic conditions resulted in long...

journal_title：Canadian journal of microbiology

authors： Zachary A

更新日期：1978-03-01 00:00:00

abstract：:Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipi...

journal_title：Canadian journal of microbiology

authors： Van Horn KG,Smibert RM

更新日期：1982-03-01 00:00:00

abstract：:Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...

journal_title：Canadian journal of microbiology

authors： Golubev WI,Sampaio JP,Alves L,Golubev NW

更新日期：2004-12-01 00:00:00

abstract：:A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicat...

journal_title：Canadian journal of microbiology

authors： Lawler GC,Weber DJ

更新日期：1977-08-01 00:00:00

abstract：:Streptomyces echinatus A8331 cultured on a maltose minimal salts medium normally produces a single antibiotic, echinomycin (quinomycin A), containing two quinoxaline-2-carbonyl chromophores. Echinomycin is powerfully active against experimental tumours and can be assayed by its activity against Gram-positive bacteria....

journal_title：Canadian journal of microbiology

authors： Gauvreau D,Waring MJ

更新日期：1984-04-01 00:00:00

abstract：:Two strains of Bacillus sphaericus. SSII-1 and 1593, were bioassayed for toxic activity against second-instar larvae of the mosquito Culex pipiens quinquefasciatus. It was found that strain 1593 developed a level of toxicity 3000 times that of strain SSII-1. Although the toxic activity of B. sphaericus SSII-1 was rela...

journal_title：Canadian journal of microbiology

authors： Myers P,Yousten AA,Davidson EW

更新日期：1979-11-01 00:00:00

abstract：:Vaccination is the most promising strategy to reduce the incidence of pneumococcal infection. Although there are vaccines available, all of them are based on polysaccharide antigens (conjugated or not). In addition to their high cost, those vaccines do not cover all serotypes. To overcome these hindrances, we evaluate...

journal_title：Canadian journal of microbiology

authors： Araújo HF,Campos PC,Camargo DR,Pereira FN,Samuel ML,Oliveira MA,Fortes-Dias CL,Leclercq SY

更新日期：2012-09-01 00:00:00

abstract：:This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration-elution methods: flatbed membranes, FiltaMax foam, Envirochek HV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining ...

journal_title：Canadian journal of microbiology

authors： Ferguson C,Kaucner C,Krogh M,Deere D,Warnecke M

更新日期：2004-09-01 00:00:00

abstract：:Fluorescein isothiocyanate-conjugated antibody to purified enterotoxin of Clostridium perfringens was used to study the intracellular formation of enterotoxin by this organism. Enterotoxin was detected at 4 h of growth at the end of the cell containing forespore. With the development of the spore, enterotoxin accumula...

journal_title：Canadian journal of microbiology

authors： Niilo L

更新日期：1977-07-01 00:00:00

abstract：:The nucleotide sequence of the murE gene encoding the diaminopimelic acid adding enzyme of Escherichia coli is reported. The coding region consisted of 1413 base pairs and was separated from the ftsI (penicillin-binding protein 3) gene by 61 base pairs. The deduced primary structure of MurE comprised 471 amino acid re...

journal_title：Canadian journal of microbiology

authors： Tao JS,Ishiguro EE

更新日期：1989-11-01 00:00:00