Abstract：
:Using a gene fusion approach, hybrid proteins were created by linking alkaline phosphatase (PhoA) or a cellulase reporter (delta CenA) to four large N-terminal portions of the Caulobacter crescentus surface (S)-layer protein (RsaA; 1026 amino acids). Three of the sites (amino acids 189, 220, 315) were selected on the basis of TnphoA experiments that suggested the first 250-350 amino acids of RsaA could mediate export of PhoA from the cytoplasm while the fourth lay only 21 amino acids from the C-terminus. Expression of all fusions except rsaA(315):delta cenA and rsaA(315):phoA was toxic to C. crescentus. None of the gene fusions were toxic when expressed by Escherichia coli DH5 alpha, where all the hybrid proteins accumulated as inclusion bodies. The toxicity of hybrid proteins encoding 189, 220, and 1005 RsaA-derived amino acids was related to the nature of the hybrid protein itself because truncated RsaA peptides lacking their reporter domains were nontoxic. Further study of RsaA(delta C21) showed that this and presumably other truncated RsaA derivatives were neither secreted nor prone to intracellular accumulation. Although C. crescentus tolerated the expression of rsaA(315):delta cenA and rsaA(315):phoA, the encoded hybrid proteins were not exported in significant quantities from the cytoplasm. These results extend and confirm earlier work that large portions of the S-layer protein N-terminus cannot mediate export of passenger proteins from the cytoplasm and that the entire native S-layer protein may be required to properly interact with the RsaA secretion machinery.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Bingle WH,Smit Jdoi
10.1139/m94-122subject
Has Abstractpub_date
1994-09-01 00:00:00pages
777-82issue
9eissn
0008-4166issn
1480-3275journal_volume
40pub_type
杂志文章abstract：:The release of 14CO2 from 14C-labelled glucose(G-1(-14)C, G-3,4(-14)C, G-6(-14)C) was followed in phased cultures of Candida utilis grown in a glucose- mineral salts medium under altered conditions of carbon:nitrogen limitation at doubling times of 2,4 and 6, h. Changes in oxygen uptake and CO2 evolution were observe...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-150
更新日期：1975-07-01 00:00:00
abstract：:Cultured alveolar and peritoneal macrophages obtained from normal and immunized rabbits were used to induce wall-defective microbial variants of Pseudomonas pseudomallei. Variants were iduced only by normal alveolar macrophages. The variants reverted to typical Pseudomonas forms either spontaneously or upon transfer i...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-303
更新日期：1975-12-01 00:00:00
abstract：:Studies of the molecular biology of human papillomavirus type 16 have been limited by the lack of a tissue culture system that is fully permissive for virus replication; as a result, high-titre stocks of infectious virus are not readily available. Therefore, studies of viral gene expression have relied on analysis of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-016
更新日期：1993-01-01 00:00:00
abstract：:The binding of mutagenic pyrolyzates to cell fractions from some gram-negative intestinal bacteria and to thermally treated bacterial cells was investigated. 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) were effectively bound by several of the bacterial ce...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-101
更新日期：1992-07-01 00:00:00
abstract：:Solid media were used to determine which extracellular hydrolytic enzymes are produced by Phytophthora parasitica; P. parasitica var. nicotianae, races 0, 1, and 3; and several other Phytophthora spp. Most isolates produced RNase, DNase, phosphatase, lipase, and cellulase uniformly. All race 3 isolates of P. parasitic...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-015
更新日期：1978-01-01 00:00:00
abstract：:The transmission of multi-drug resistant pathogens and antimicrobial-resistant genes is an arising problem with multiple factors involved (humans, domestic animals, wildlife). The aim of this study was to investigate the presence of Escherichia coli isolates with different antimicrobial resistance genes from backyard ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0280
更新日期：2021-01-04 00:00:00
abstract：:Growth rates and cell yields of Mycobacterium convolutum strain R22 grown on a wide range of both odd- and even-numbered carbon solid n-alkanes decreased as the substrate carbon number increased. Cellular lipid was 2.5 times higher following growth on the hydrocarbon substrates. The amount of polar lipid was found to ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-194
更新日期：1978-10-01 00:00:00
abstract：:Alkaline phosphatase (APase) was present in the bovine rumen in both cell-free and cell-associated states and levels of the enzyme varied with dietary regime. Reaction product deposition showed that the enzyme was associated with the mixed bacterial population. No enzyme was observed to be associated with protozoa. Tr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-111
更新日期：1976-05-01 00:00:00
abstract：:Soil bacteria that utilize inositol hexaphosphate (IHP) were isolated from a range of soils using defined selection media. An analysis of 200 randomly selected isolates indicated that less than 0.5% of the culturable population of soil bacteria were capable of using IHP as a sole source of C and P. From a further 238 ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-073
更新日期：1997-06-01 00:00:00
abstract：:Absorption of 14C-labelled poliovirus-2 to sedimentable solids of primary sludge samples collected from a secondary treatment facility during a 6-month period averaged 94%; for anaerobically digested sludge, 99%. The extent of virus adsorption was influenced by the amount of solids. Maximal adsorption occurred at or a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-254
更新日期：1983-12-01 00:00:00
abstract：:In a previous study it was shown that the number of wood-inhabiting bacteria was drastically reduced after colonization of beech (Fagus sylvatica) wood blocks by the white-rot fungus Hypholoma fasciculare, or sulfur tuft (Folman et al. 2008). Here we report on the mechanisms of this fungal-induced antibacterial activi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-023
更新日期：2010-05-01 00:00:00
abstract：:The use of microorganisms for bioremediation of contaminated soils may be enhanced with an understanding of the pathways involved in their degradation of hazardous compounds. Ralstonia sp. strain RJGII.123 was isolated from soil located at a former coal gasification plant, based on its ability to mineralize carbazole,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w99-131
更新日期：2000-03-01 00:00:00
abstract：:The sensitivity of delta red-gam delta ren mutants of bacteriophage lambda to Rex exclusion by lambda rexA+ rexB+ lysogens is modulated by the prophage cI repressor allele. We show the following: (i) lambda spi156 delta nin5 forms plaques on a cI+-rexA+-rexB+ lysogen with 10(5)-fold higher efficiency than on cI[Ts]-re...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-021
更新日期：2003-03-01 00:00:00
abstract：:Attempts at improving chemical fixation for electron-microscopic observation of the capsule of Klebsiella pneumoniae were made. The capsule was preserved by using alcian blue - lanthanum and tris-(1-aziridinyl) phosphine oxide (TAPO) - aldehyde - osmium procedures. Despite the different retention of the overall capsul...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-102
更新日期：1977-06-01 00:00:00
abstract：:Pestalotiopsis species were most dominant endophytic species isolated from four medicinal plants including Terminalia arjuna, Terminalia chebula, Azadirachta indica, and Holarrhena antidysenterica. Thirty Pestalotiopsis species isolated from different parts of the medicinal plants were selected for the study. The anti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-070
更新日期：2008-09-01 00:00:00
abstract：:The bacterial bioluminescence assay is one of the novel means for toxicity detection. The bioluminescence response of 2 marine bioluminescent bacteria was tested upon their long-term exposure to 9 different reverse osmosis (RO) rejects with varying chemical composition sampled from various dye industries. Bioluminesce...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0378
更新日期：2014-10-01 00:00:00
abstract：:Highly distinctive aspects of the exponentail-phase Rhizobium japonicum cell were disclosed by means of thin sections, freeze etching, fluorescent antibodies, and ruthenium red staining. Polarity was expressed in the form of reserve polymer distribution near one end of the cell and as cytoplasmic localization near the...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-191
更新日期：1977-09-01 00:00:00
abstract：:The catalytic subunit of many Escherichia coli redox enzymes bares a twin-arginine translocation (Tat)-dependent signal peptide in its precursor, which directs the redox enzyme complex to this Sec-independent pathway. NarG of the E. coli nitrate reductase NarGHI complex possesses a vestige twin-arginine motif at its N...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-111
更新日期：2009-02-01 00:00:00
abstract：:Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDREI) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenoty...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-004
更新日期：2003-01-01 00:00:00
abstract：:We examined factors related to the potent antagonistic effect of Escherichia coli and Bacteroides ovatus on Staphylococcus aureus in anaerobic continuous flow cultures. In the presence of sugars fermentable by E. coli alone or both E. coli and S. aureus, motile E. coli strains exerted a potent antagonistic effect and ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-106
更新日期：1988-05-01 00:00:00
abstract：:Andosols comprise one of the most important soil groups for agricultural activities in Japan because they cover about 46.5% of arable upland fields. In this soil group, available phosphorus (P) is accumulated by application of excessive fertilizer, but little is known about the influence of increasing P availability o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0612
更新日期：2013-06-01 00:00:00
abstract：:Ten strains of Caldariomyces fumago and related fungi were found to produce extracellular chloroperoxidase when grown on a glucose - malt extract medium. High enzyme levels and pigment production were observed for C. fumago ATCC 16373 and C. fumago CMI 89362. Removal of malt extract from the medium and the replacement...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-199
更新日期：1981-12-01 00:00:00
abstract：:The environmental distribution of Giardia spp. and Cryptosporidium spp. is dependent upon human, agricultural, and wildlife sources. The significance of each source with regard to the presence of parasites in the environment is unknown. This 2-year study examined parasite prevalence in human sewage influent, wildlife,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-047
更新日期：2002-06-01 00:00:00
abstract：:The immune benefits associated with the optimal intake of dietary fatty acids are widely known. The objective of the present investigation was to elucidate the role of omega-3 polyunsaturated fatty acids (n-3 PUFA) food source on acute pneumonia induced by Klebsiella pneumoniae. Three different n-3 PUFA preparations (...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0521
更新日期：2013-07-01 00:00:00
abstract：:Twenty-six strains of group JK corynebacteria had the same colonial morphology and biological reactions as the biotypes of the biovars of Corynebacterium genitalium and C. pseudogenitalium. Therefore, group JK corynebacteria can be assigned to the biovars of C. genitalium or C. pseudogenitalium. Although the strains d...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-164
更新日期：1984-08-01 00:00:00
abstract：:A baseline serotype distribution was established by age and region for 2058 invasive Streptococcus pneumoniae isolates collected during the implementation period of the 13-valent pneumococcal conjugate vaccine (PCV13) program in many parts of Canada in 2010. Serotypes 19A, 7F, and 3 were the most prevalent in all ag...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-073
更新日期：2012-08-01 00:00:00
abstract：:alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-095
更新日期：1986-06-01 00:00:00
abstract：:When Caryophanon latum was exposed to egg white lysozyme in isotonic sucrose and observed by phase-contrast microscopy, protoplasts emerged along the length of the trichomes, apparently at sites corresponding to cross septa. Electron microscopy of sections revealed that this enzyme initially attacked the core of the s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-024
更新日期：1975-02-01 00:00:00
abstract：:Dietary exposure to heavy metals may have detrimental effects on human and animal health, even at low concentrations. Specific probiotic bacteria may have properties that enable them to bind toxins from food and water. We assessed the interaction of probiotic bacteria with cadmium and lead in vitro as an initial scree...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-043
更新日期：2006-09-01 00:00:00
abstract：:In mycetomes of leafhoppers, Helochara communis, ultrastructural and histochemical studies revealed that spheroplast symbiotes (t-symbiotes) were converted to 'a-symbiotes' (so-called), with apparent loss of DNA--a phenomenon reminiscent of rhizobium-bacteroid conversion in certain legume nodules. Additional t-symbiot...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-028
更新日期：1975-02-01 00:00:00
