Abstract:
:The transmission of multi-drug resistant pathogens and antimicrobial-resistant genes is an arising problem with multiple factors involved (humans, domestic animals, wildlife). The aim of this study was to investigate the presence of Escherichia coli isolates with different antimicrobial resistance genes from backyard poultry and demonstrate the in vitro transduction phenomenon of these genes between phages from migratory wild-birds and poultry E. coli isolates. We collected 197 E. coli isolates from chicken, turkeys, and ducks in backyard production units (northern region of the State of Mexico). Isolates were resistant to ampicillin (80.7%), tetracycline (64.4%), carbenicillin (56.3%), and nalidixic acid and trimethoprim-sulfamethoxazole (both, 26.9%). Moreover, blaTEM (56.3%), tetB (20.8%), tetA (19.2%), sulI (7.6%), sulII (10.1%), qnrA (9.6%) and qnrB (5.5%) genes were found. In vitro transduction using phages from migratory wild birds sampled in the wetland Chimaliapan (State of Mexico) was worked out. It was possible to transduce qnrA, tetB, blaTEM and sulII genes to E. coli isolates from poultry. This is the first report that describes the transduction of antimicrobial resistance genes from phages of migratory wild birds to poultry and suggests the possible transmission in backyard production units.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Talavera-Gonzalez JM,Talavera-Rojas M,Soriano-Vargas E,Vazquez-Navarrete J,Salgado-Miranda Cdoi
10.1139/cjm-2020-0280subject
Has Abstractpub_date
2021-01-04 00:00:00eissn
0008-4166issn
1480-3275pub_type
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