Abstract:
:A high molecular component of a saline extract derived from Listeria monocytogenes contained amino acids, carbohydrates, and phosphorus. The same fraction was capable of promoting both the in vitro mitogenic and adjuvant activities and the in vivo immunosuppressive activity displayed by the crude extract. The material was mitogenic to B but not to T lymphocytes in vitro. Responses to sheep and horse erythrocytes as well as to lipopolysaccharide were suppressed. Immunosuppression was dose dependent and was present at 1, 2, or 3 days but absent 7 days after injection. Both primary and secondary responses to sheep erythrocytes were impaired.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Otokunefor TV,Galsworthy SBdoi
10.1139/m82-204subject
Has Abstractpub_date
1982-12-01 00:00:00pages
1373-81issue
12eissn
0008-4166issn
1480-3275journal_volume
28pub_type
杂志文章abstract::A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-162
更新日期:1997-12-01 00:00:00
abstract::A rolling-circle amplification (RCA) method with padlock probes targeted on EF-1α regions was developed for rapid detection of apple bull's-eye rot pathogens, including Neofabraea malicorticis, N. perennans, N. kienholzii, and N. vagabunda (synonym: N. alba). Four padlock probes (PLP-Nm, PLP-Np, PLP-Nk, and PLP-Nv) we...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2017-0448
更新日期:2018-01-01 00:00:00
abstract::Bacillus stearothermophilus NCA 2184 lost viability and subsequently released cytoplasmic components when suspended in 0.1 M tris(hydroxymethyl)aminomethane (Tris) buffer (pH 7.2) and incubated at 60 degrees C. Cell lysis was prevented by the addition of 10 mM CaCl2 to the Tris-buffer suspension. Cells which were incu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-073
更新日期:1976-04-01 00:00:00
abstract::Nodulation of Pisum sativum cv. Afghanistan by Rhizobium leguminosarum strain Tom can be blocked by the nonnodulating R. leguminosarum strain PF2. This system was used to study the dynamics of rhizobial binding to seedling root surfaces. Appreciable numbers of 35S-labeled rhizobia bound to the root surface within 2h a...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-099
更新日期:1980-04-01 00:00:00
abstract::Plant secondary metabolites, such as saponins, have a considerable impact in agriculture because of their allelopathic effects. They also affect the growth of soil microorganisms, especially fungi. We investigated the influence of saponins on rhizosphere bacteria in vitro and in soil conditions. The effects of gypsoph...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-052
更新日期:2003-06-01 00:00:00
abstract::A proline dehydrogenase which catalyzes the nicotinamide adenine dinucleotide (NAD) dependent oxidation of proline and the NADH-dependent reduction of delta 1-pyrroline 5-carboxylic acid (PCA) was purified from extracts of Clostridium sporogenes. Following purification, only one protein band was found on analytical po...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-147
更新日期:1981-09-01 00:00:00
abstract::We examined isolates from 4 commercial bioinsecticides based on different strains of Bacillus thuringiensis subspecies (kurstaki, israelensis, aizawai, and tenebrionis) for the presence of genes encoding proteins with known enterotoxigenicity (nhe, hbl, cytk, ces) and various other putative virulence genes (piplc, sph...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-106
更新日期:2007-12-01 00:00:00
abstract::The open reading frame pp0053, which has a high homology with the sequence of mitochondrial sulfide dehydrogenase (HMT2) conferring cadmium tolerance in fission yeast, was amplified from Pseudomonas putida KT2440 and expressed in Escherichia coli JM109(DE3). The isolated and purified PP0053-His showed absorption spect...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-022
更新日期:2006-08-01 00:00:00
abstract::The fatty acid pattern in Mycobacterium vaccae strain JOB5 was examined after growth on n-alkanes (C14-C18), 1-alkenes (C14-C18), 2- or 3-methyl octadecane, and 8-heptadecene. It was evident that monoterminal oxidation of n-alkanes was followed by beta-oxidation and that both parent fatty acid and products of beta-oxi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-012
更新日期:1975-01-01 00:00:00
abstract::The effects of water activity (aw, 0.994-0.90 identical to 0.4-14.0 (-)MPa water potential), temperature (4-45 degrees C), and pH (3.6, 5.5, 7.0), and their interactions on growth of isolates of Fusarium moniliforme and Fusarium proliferatum were determined in vitro on a maize extract agar medium. Growth of two isolat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-149
更新日期:1995-12-01 00:00:00
abstract::In the past decade, the large tailings pond (Mildred Lake Settling Basin) on the Syncrude Canada Ltd. lease near Fort McMurray, Alta., has gone methanogenic. Currently, about 60%-80% of the flux of gas across the surface of the tailings pond is methane. As well as adding to greenhouse gas emissions, the production of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::The initial cause of the cessation of patulin biosynthesis in submerged cultures of Penicillium urticae was sought. The extrinsic limitations to patulin biosynthesis in fermentor cultures were successfully removed and kinetic profiles of the metabolites of the pathway and of three pathway enzymes were determined over ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-032
更新日期:1981-02-01 00:00:00
abstract::Our purpose was to study bacterial antagonism between a limited number of strictly anaerobic strains and Clostridium perfringens in the intestinal tract of gnotobiotic rodents. Gnotobiotic mice harboring a Bacteroides thetaiotaomicron, a Fusobacterium necrogenes, and a Clostridium sp. strain were protected against pat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-039
更新日期:1987-03-01 00:00:00
abstract::Mice immunized subcutaneously with 10(6) viable yeast cells of Histoplasma capsulatum exhibited host protection to a challenge infection and also exhibited concomitant manifestations of cellular immunity to a culture filtrate antigen (yeast extract dialysate (YED)-histoplasmin). Protective immunity was determined by q...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-016
更新日期:1983-01-01 00:00:00
abstract::The anaerobic degradative pathway for metabolism of 2,4,6-trinitrotoluene (TNT) by a consortium of Desulfovibrio spp. isolated from a creek sediment was studied. This consortium has the metabolic capability to degrade TNT to fatty acids. The growth of the consortium and the metabolism of TNT were greatly enhanced in t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-155
更新日期:1996-12-01 00:00:00
abstract::In British Columbia, Canada, drinking water is tested for total coliforms and Escherichia coli, but there is currently no routine follow-up testing to investigate fecal contamination sources in samples that test positive for indicator bacteria. Reliable microbial source tracking (MST) tools to rapidly test water sampl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0618
更新日期:2013-03-01 00:00:00
abstract::A comparative study of the pathogenicity of Candida albicans morphological mutant strains was made to relate chlamydospore production, germ tube formation, and proteolytic activity to candidiasis in mice. It was observed that the mycelium strains were more lethal than the yeast-like strains and that neither chlamydosp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-093
更新日期:1975-05-01 00:00:00
abstract::Approximately 20% of Canada's oil supply now comes from the extraction of bitumen from the oil sands deposits in northeastern Alberta. The oil sands are strip-mined, and the bitumen is typically separated from sand and clays by an alkaline hot water extraction process. The rapidly expanding oil sands industry has mill...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-129
更新日期:2002-01-01 00:00:00
abstract::Citrate synthase (citrate-oxaloacetate lyase (CoA acetylating), EC 4.1.3.7) has been purified to electrophoretic homogeneity from a marine Pseudomonas. The enzyme was made up of identical subunits, with a molecular wieght of about 53 000, as determined by sodium dodecyl sulphate - polyacrylamide gel electrophoresis. T...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-039
更新日期:1978-03-01 00:00:00
abstract::The induction of nitrogenase (C2H2) activity in asymbiotically cultured Rhizobium sp. 32H1 was found to be associated with morphological changes in the cells which were more pronounced than those seen in bacteroids. Polyphosphate granules were found in both bacteroids and cultured cells, but poly-beta-hydroxybutyrate ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-055
更新日期:1979-03-01 00:00:00
abstract::In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L. reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0007
更新日期:2015-06-01 00:00:00
abstract::Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obta...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-107
更新日期:1988-05-01 00:00:00
abstract::A large pool of antibiotic resistant and auxotrophic mutants was isolated from the Staphylococcus aureus phage group 2 strains UT0002-19 and UT0017 by (1) antibiotic gradient plates, (2) trimethoprim selection, and (3) nitrosoguanidine mutagenesis, which sometimes was coupled by enrichment with either penicillin or me...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-161
更新日期:1980-08-01 00:00:00
abstract::The structures of lipopolysaccharides (LPSs) of lic1 and lic1/lic2 mutants from Haemophilus influenzae type b strain Eagan (RM153) were investigated using methylation analysis, electrospray ionization - mass spectrometry, and nuclear magnetic resonance spectroscopy on O-deacylated, O- and N-deacylated core oligosaccha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-009
更新日期:2008-04-01 00:00:00
abstract::This study, the first of arbovirus activity in Prince Edward Island, has shown antibodies to the snowshoe hare strain of the California encephalitis group virus to be present in sera of wild and domestic animals; 15.35% of snowshoe hare, 20% of equine. and 2.5% of bovine sera were positive for hemagglutination inhibit...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-188
更新日期:1981-11-01 00:00:00
abstract::Fine hair-like appendages on the cell walls of the another smut Ustilago violacea are described. These hairs are termed fimbriae because of their close similarity to the fimbriae (pili) found on certain Gram-negative bacteria. Cells of U. violacea may carry more than 200 fimbriae varying in length from about 0.5 mum t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-076
更新日期:1975-04-01 00:00:00
abstract::The present investigation was aimed to scrutinize the salt tolerance potential of plant-growth-promoting rhizobacteria (PGPR) isolated from rhizospheric soil of selected halophytes (Atriplex leucoclada, Haloxylon salicornicum, Lespedeza bicolor, Suaeda fruticosa, and Salicornica virginica) collected from high-saline f...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0668
更新日期:2015-04-01 00:00:00
abstract::Microsclerotia of three melanin-deficient mutants of Verticillium dahliae formed malanin from (+)-scytalone, 1,8-dihydroxynaphthalene, catechol, and L-3,4-dihydroxyphenylalanine. The melanins formed from (+)-scytalone or 1,8-dihydroxynaphthalene resembled wild-type melanin chemically and ultrastructurally, whereas the...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-049
更新日期:1978-03-01 00:00:00
abstract::The N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum strain Marburg has been purified with reasonable yield and much higher specific activity than previously reported. For the first time it has been shown that both N5,N10-methylenetetrahydromethanopterin dehydrogenase and...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-077
更新日期:1989-04-01 00:00:00
abstract::This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjec...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-083
更新日期:2007-10-01 00:00:00